(Circular) (Six-base) MAPSORT of: pFN21ASDA0457.seq Check: 37 from: 1 to: 7225 pFN21ASDA0457 7225 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2119 Size: 7225 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5035 278 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4568 1937 Size: 2631 4594 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 5352 5725 1563 Size: 3789 373 3063 Fragments arranged by size: 3789 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2803 3077 3086 3383 5288 6672 6940 1632 Size: 1171 274 9 297 1905 1384 268 1917 Fragments arranged by size: 1917 1905 1384 1171 297 274 268 9 AflII C'TTAA_G Cuts at: 820 1017 3079 4046 820 Size: 197 2062 967 3999 Fragments arranged by size: 3999 2062 967 197 AflIII A'CryG_T Cuts at: 1203 3369 6124 7101 1203 Size: 2166 2755 977 1327 Fragments arranged by size: 2755 2166 1327 977 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 5954 5954 Size: 7225 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2420 2478 2722 3605 4501 1184 Size: 1236 58 244 883 896 3908 Fragments arranged by size: 3908 1236 896 883 244 58 AlwNI CAG_nnn'CTG Cuts at: 2177 4968 6540 2177 Size: 2791 1572 2862 Fragments arranged by size: 2862 2791 1572 ApaLI G'TGCA_C Cuts at: 5282 6438 5282 Size: 1156 6069 ApoI r'AATT_y Cuts at: 1493 3728 4534 4760 4912 7021 1493 Size: 2235 806 226 152 2109 1697 Fragments arranged by size: 2235 2109 1697 806 226 152 AseI AT'TA_AT Cuts at: 160 1032 5779 160 Size: 872 4747 1606 Fragments arranged by size: 4747 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 2290 4550 1870 Size: 73 347 2260 4545 Fragments arranged by size: 4545 2260 347 73 BamHI G'GATC_C Cuts at: 2938 4555 4922 2938 Size: 1617 367 5241 Fragments arranged by size: 5241 1617 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2396 4546 6001 618 Size: 325 217 627 258 351 2150 1455 1842 Fragments arranged by size: 2150 1842 1455 627 351 325 258 217 BanII G_rGCy'C Cuts at: 1391 2315 2747 4401 4544 1391 Size: 924 432 1654 143 4072 Fragments arranged by size: 4072 1654 924 432 143 BbeI G_GCGC'C Cuts at: 2049 2049 Size: 7225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3171 4256 4311 928 Size: 971 1272 1085 55 3842 Fragments arranged by size: 3842 1272 1085 971 55 BbvCI CC'TCA_GC Cuts at: 3607 3896 3607 Size: 289 6936 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5416 5450 1903 Size: 34 3479 34 3678 Fragments arranged by size: 3678 3479 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6327 5117 Size: 1210 6015 BclI T'GATC_A Cuts at: 1548 1548 Size: 7225 BfrBI ATG'CAT Cuts at: 2896 3802 2896 Size: 906 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2413 5836 136 Size: 107 122 71 631 605 741 3423 1525 Fragments arranged by size: 3423 1525 741 631 605 122 107 71 BglII A'GATC_T Cuts at: 4372 7220 4372 Size: 2848 4377 BlpI GC'TnA_GC Cuts at: 2417 4642 2417 Size: 2225 5000 Bme1580I G_kGCm'C Cuts at: 1790 2210 5286 6442 1790 Size: 420 3076 1156 2573 Fragments arranged by size: 3076 2573 1156 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4956 5914 442 Size: 416 53 83 399 24 3539 958 1753 Fragments arranged by size: 3539 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2860 2911 3497 3644 3905 4127 4385 4427 Size: 1621 51 586 147 261 222 258 42 Cuts at: 4427 5885 1239 Size: 1458 2579 Fragments arranged by size: 2579 1621 1458 586 261 258 222 147 51 42 Bpu10I CC'TnA_GC Cuts at: 2980 3011 3354 3607 3896 2980 Size: 31 343 253 289 6309 Fragments arranged by size: 6309 343 289 253 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2629 5350 6215 6513 2629 Size: 2721 865 298 3341 Fragments arranged by size: 3341 2721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 2845 5888 882 Size: 617 978 368 3043 2219 Fragments arranged by size: 3043 2219 978 617 368 BsaAI yAC'GTr Cuts at: 493 1204 3370 4522 5016 493 Size: 711 2166 1152 494 2702 Fragments arranged by size: 2702 2166 1152 711 494 BsaBI GATnn'nnATC Cuts at: 4371 7219 4371 Size: 2848 4377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2493 5032 5414 Size: 53 83 186 936 513 447 2539 382 Cuts at: 5414 6049 275 Size: 635 1451 Fragments arranged by size: 2539 1451 936 635 513 447 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 2307 3054 3640 5658 6056 6330 6477 6888 Size: 90 747 586 2018 398 274 147 411 Cuts at: 6888 2217 Size: 2554 Fragments arranged by size: 2554 2018 747 586 411 398 274 147 90 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2906 2936 1178 Size: 30 165 30 1503 30 5467 Fragments arranged by size: 5467 1503 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2914 3509 3863 3884 4200 1186 Size: 1072 656 595 354 21 316 4211 Fragments arranged by size: 4211 1072 656 595 354 316 21 BseYI C'CCAG_C Cuts at: 2050 2197 2444 2499 3937 6428 6924 2050 Size: 147 247 55 1438 2491 496 2351 Fragments arranged by size: 2491 2351 1438 496 247 147 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 3176 4081 1163 Size: 573 613 827 905 4307 Fragments arranged by size: 4307 905 827 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4675 5436 6464 1540 Size: 275 186 2674 761 1028 2301 Fragments arranged by size: 2674 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3987 4401 5286 5371 6442 3987 Size: 414 885 85 1071 4770 Fragments arranged by size: 4770 1071 885 414 85 BsmI GAATG_Cn' Cuts at: 2896 4736 4829 2896 Size: 1840 93 5292 Fragments arranged by size: 5292 1840 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3137 3376 3137 Size: 239 6986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2315 2747 3987 4401 4544 5286 Size: 399 420 105 432 1240 414 143 742 Cuts at: 5286 5371 6442 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1240 1071 742 432 420 414 399 143 105 85 BspEI T'CCGG_A Cuts at: 2307 6888 2307 Size: 4581 2644 BspHI T'CATG_A Cuts at: 4363 5114 6844 4363 Size: 751 1730 4744 Fragments arranged by size: 4744 1730 751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 4580 844 Size: 1275 2461 3489 Fragments arranged by size: 3489 2461 1275 BsrBI CCG'CTC Cuts at: 1394 1823 5112 1394 Size: 429 3289 3507 Fragments arranged by size: 3507 3289 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2734 5720 5894 65 Size: 1183 1486 2986 174 1396 Fragments arranged by size: 2986 1486 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 3611 5869 6056 1700 Size: 110 250 1551 2258 187 2869 Fragments arranged by size: 2869 2258 1551 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7225 BssSI C'ACGA_G Cuts at: 2789 3379 5285 6297 2789 Size: 590 1906 1012 3717 Fragments arranged by size: 3717 1906 1012 590 BstAPI GCAn_nnn'nTGC Cuts at: 794 2580 794 Size: 1786 5439 BstBI TT'CG_AA Cuts at: 2007 6029 2007 Size: 4022 3203 BstEII G'GTnAC_C Cuts at: 1184 3239 1184 Size: 2055 5170 BstXI CCAn_nnnn'nTGG Cuts at: 2935 2935 Size: 7225 BstYI r'GATC_y Cuts at: 1972 2938 4372 4555 4922 5307 5324 6776 6862 Size: 966 1434 183 367 385 17 1452 86 Cuts at: 6862 6874 7220 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1434 966 385 367 346 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2601 3743 2040 Size: 561 1142 5522 Fragments arranged by size: 5522 1142 561 BtgI C'CryG_G Cuts at: 513 1065 2469 3274 513 Size: 552 1404 805 4464 Fragments arranged by size: 4464 1404 805 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2532 2815 487 Size: 683 56 1306 283 4897 Fragments arranged by size: 4897 1306 683 283 56 BtsI GCAGTG_nn' Cuts at: 2208 3348 4076 4741 5535 5555 2208 Size: 1140 728 665 794 20 3878 Fragments arranged by size: 3878 1140 794 728 665 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3497 3532 529 Size: 35 2933 35 4222 Fragments arranged by size: 4222 2933 35 35 DraI TTT'AAA Cuts at: 4529 4529 Size: 7225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6232 809 Size: 507 4916 1802 Fragments arranged by size: 4916 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2423 4672 5561 Size: 54 1425 50 275 18 593 2249 889 Cuts at: 5561 7190 8 Size: 1629 43 Fragments arranged by size: 2249 1629 1425 889 593 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4672 1537 Size: 275 2860 4090 Fragments arranged by size: 4090 2860 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3967 5155 1353 Size: 2614 1188 3423 Fragments arranged by size: 3423 2614 1188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5812 6186 6332 387 Size: 1064 138 4223 374 146 1280 Fragments arranged by size: 4223 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4399 4399 Size: 7225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2803 2860 2911 3077 3086 3383 3497 Size: 393 1171 57 51 166 9 297 114 Cuts at: 3497 3644 3905 4127 4385 4427 5288 5885 6672 Size: 147 261 222 258 42 861 597 787 Cuts at: 6672 6940 1239 Size: 268 1524 Fragments arranged by size: 1524 1171 861 787 597 393 297 268 261 258 222 166 147 114 57 51 42 9 EcoNI CCTnn'n_nnAGG Cuts at: 3845 3845 Size: 7225 EcoO109I rG'GnC_Cy Cuts at: 2036 2174 2605 2794 2036 Size: 138 431 189 6467 Fragments arranged by size: 6467 431 189 138 EcoRI G'AATT_C Cuts at: 4534 4912 4534 Size: 378 6847 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4358 4358 Size: 7225 FspI TGC'GCA Cuts at: 5731 5731 Size: 7225 HaeII r_GCGC'y Cuts at: 1678 2049 6372 1678 Size: 371 4323 2531 Fragments arranged by size: 4323 2531 371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2714 2746 2906 2938 2978 Size: 32 135 32 1142 32 160 32 40 Cuts at: 2978 3010 3059 3091 3842 3874 4110 4142 5866 Size: 32 49 32 751 32 236 32 1724 Cuts at: 5866 5898 5940 5972 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1724 1142 751 236 160 135 49 42 40 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3757 4569 4815 669 Size: 988 281 1819 812 246 3079 Fragments arranged by size: 3079 1819 988 812 281 246 HindIII A'AGCT_T Cuts at: 748 2647 4110 748 Size: 1899 1463 3863 Fragments arranged by size: 3863 1899 1463 HpaI GTT'AAC Cuts at: 3757 4815 3757 Size: 1058 6167 KasI G'GCGC_C Cuts at: 2045 2045 Size: 7225 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 4824 4824 Size: 7225 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3321 6339 6523 1305 Size: 2016 3018 184 2007 Fragments arranged by size: 3018 2016 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5403 Fragments arranged by size: 5403 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4362 5183 5542 5701 518 Size: 648 3196 821 359 159 2042 Fragments arranged by size: 3196 2042 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2499 2580 2976 3297 3478 3553 3568 4065 Size: 893 81 396 321 181 75 15 497 Cuts at: 4065 4496 4641 5318 6466 6711 1606 Size: 431 145 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 677 497 431 396 321 245 181 145 81 75 15 NaeI GCC'GGC Cuts at: 2062 2062 Size: 7225 NarI GG'CG_CC Cuts at: 2046 2046 Size: 7225 NcoI C'CATG_G Cuts at: 513 1065 2469 513 Size: 552 1404 5269 Fragments arranged by size: 5269 1404 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7225 NgoMIV G'CCGG_C Cuts at: 2060 2060 Size: 7225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7225 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NsiI A_TGCA'T Cuts at: 2898 3804 2898 Size: 906 6319 NspI r_CATG'y Cuts at: 1132 1229 2896 3806 4583 6128 1132 Size: 97 1667 910 777 1545 2229 Fragments arranged by size: 2229 1667 1545 910 777 97 PasI CC'CwG_GG Cuts at: 1348 2435 3559 1348 Size: 1087 1124 5014 Fragments arranged by size: 5014 1124 1087 PciI A'CATG_T Cuts at: 6124 6124 Size: 7225 PflMI CCAn_nnn'nTGG Cuts at: 1108 2755 4082 1108 Size: 1647 1327 4251 Fragments arranged by size: 4251 1647 1327 PfoI T'CCnGG_A Cuts at: 3660 3920 4143 3660 Size: 260 223 6742 Fragments arranged by size: 6742 260 223 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PmlI CAC'GTG Cuts at: 3370 3370 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5294 5326 6807 6839 5294 Size: 32 1481 32 5680 Fragments arranged by size: 5680 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 2605 2794 2036 Size: 569 189 6467 Fragments arranged by size: 6467 569 189 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7225 PsiI TTA'TAA Cuts at: 4795 4795 Size: 7225 PstI C_TGCA'G Cuts at: 830 2116 2516 2585 2848 3573 3737 4285 4425 Size: 1286 400 69 263 725 164 548 140 Cuts at: 4425 4440 4480 4577 830 Size: 15 40 97 3478 Fragments arranged by size: 3478 1286 725 548 400 263 164 140 97 69 40 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 2499 2580 3478 3553 3568 4065 4496 2499 Size: 81 898 75 15 497 431 5228 Fragments arranged by size: 5228 898 497 431 81 75 15 SacI G_AGCT'C Cuts at: 4401 4401 Size: 7225 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 5473 7185 5473 Size: 1712 5513 SfcI C'TryA_G Cuts at: 826 1045 2112 2512 2581 2844 3416 3569 3733 Size: 219 1067 400 69 263 572 153 164 Cuts at: 3733 4136 4167 4281 4421 4436 4476 4573 5708 Size: 403 31 114 140 15 40 97 1135 Cuts at: 5708 6389 6580 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1067 681 572 403 400 263 219 191 164 153 140 114 97 69 40 31 15 SfoI GGC'GCC Cuts at: 2047 2047 Size: 7225 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 2060 1700 Size: 360 6865 SmaI CCC'GGG Cuts at: 1872 4552 1872 Size: 2680 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2290 2644 3079 4046 5329 6230 Size: 197 926 347 354 435 967 1283 901 Cuts at: 6230 6492 820 Size: 262 1553 Fragments arranged by size: 1553 1283 967 926 901 435 354 347 262 197 SnaBI TAC'GTA Cuts at: 493 4522 5016 493 Size: 4029 494 2702 Fragments arranged by size: 4029 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 3806 4583 1132 Size: 2674 777 3774 Fragments arranged by size: 3774 2674 777 SspI AAT'ATT Cuts at: 5 52 5149 5 Size: 47 5097 2081 Fragments arranged by size: 5097 2081 47 StuI AGG'CCT Cuts at: 1517 2298 3587 1517 Size: 781 1289 5155 Fragments arranged by size: 5155 1289 781 StyI C'CwwG_G Cuts at: 513 1065 2469 4664 513 Size: 552 1404 2195 3074 Fragments arranged by size: 3074 2195 1404 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2436 3323 5252 5269 5422 5607 6052 Size: 1255 856 887 1929 17 153 185 445 Cuts at: 6052 325 Size: 1498 Fragments arranged by size: 1929 1498 1255 887 856 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4032 4326 5471 Size: 274 80 33 51 1443 2055 294 1145 Cuts at: 5471 7183 96 Size: 1712 138 Fragments arranged by size: 2055 1712 1443 1145 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 7185 7217 2246 Size: 32 4907 32 2254 Fragments arranged by size: 4907 2254 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7225 XbaI T'CTAG_A Cuts at: 4561 4561 Size: 7225 XhoI C'TCGA_G Cuts at: 1943 2290 1943 Size: 347 6878 XmaI C'CCGG_G Cuts at: 1870 4550 1870 Size: 2680 4545 XmnI GAAnn'nnTTC Cuts at: 5354 5354 Size: 7225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5033 276 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI