Restriction map for
pFN21ASDA0448
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaI AvaII AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TfiI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 60 CGCG CG^CG
AflIII 423 ACACGT A^C[GA][CT]GT
AhdI 140 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 9 CCTCAGG CC^T[ACGT]AGG
AspEI 140 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 626 GTGCTC G[AT]GC[AT]^C
BalI 666 TGGCCA TGG^CCA
BamHI 492 GGATCC G^GATCC
BclI 224 TGATCA T^GATCA
BcnI 806 CCCGG CC^[GC]GG
BsaI 1010 GAGACC GGTCTC(1/5)
BsiHKAI 626 GTGCTC G[AT]GC[AT]^C
BsmBI 564 GAGACG CGTCTC(1/5)
BsmFI 108 GTCCC GGGAC(10/14)
BsmI 679 GAATGC GAATGC(1/-1)
BspMI 698 GCAGGT ACCTGC(4/8)
BsrBI 111 CCGCTC CCGCTC(-3/-3)
BstUI 60 CGCG CG^CG
Bsu36I 9 CCTCAGG CC^T[ACGT]AGG
CfoI 366 GCGC GCG^C
Cfr13I 181 GGCCC G^G[ACGT]CC
EaeI 666 TGGCCA [CT]^GGCC[GA]
Eam1105I 140 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 9 CCTCAGG CC^T[ACGT]AGG
FauI 110 CCCGC CCCGC(4/6)
FbaI 224 TGATCA T^GATCA
HapII 807 CCGG C^CGG
HhaI 366 GCGC GCG^C
HinP1I 366 GCGC G^CGC
HinfI 106 GAGTC G^A[ACGT]TC
HpaII 807 CCGG C^CGG
HphI 222 GGTGA GGTGA(8/7)
MaeII 425 ACGT A^CGT
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
MspA1I 37 CAGCTG C[AC]G^C[GT]G
MspI 807 CCGG C^CGG
MvnI 60 CGCG CG^CG
NciI 806 CCCGG CC^[GC]GG
PflMI 93 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 106 GAGTC GAGTC(4/5)
PvuII 37 CAGCTG CAG^CTG
SapI 72 GCTCTTC GCTCTTC(1/4)
Sau96I 181 GGCCC G^G[ACGT]CC
SfcI 910 CTACAG C^T[GA][CT]AG
SspI 740 AATATT AAT^ATT
TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 93 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 631 CCAGAGAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 448 AAATTT [GA]^AATT[CT]
952 GAATTT
AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
94 CAGAAACTG
ApoI 448 AAATTT [GA]^AATT[CT]
952 GAATTT
AseI 478 ATTAAT AT^TAAT
733 ATTAAT
AsnI 478 ATTAAT AT^TAAT
733 ATTAAT
Asp700I 739 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAATGAGTTC
BanII 4 GGGCTC G[GA]GC[CT]^C
983 GAGCCC
BpmI 629 CTCCAG CTGGAG(16/14)
100 CTGGAG
BseRI 7 CTCCTC GAGGAG(10/8)
103 GAGGAG
BsiYI 93 CCAGAAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
677 CCGAATGCTGG
BslI 93 CCAGAAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
677 CCGAATGCTGG
BsrI 99 ACTGG ACTGG(1/-1)
340 CCAGT
BstF5I 1020 CATCC GGATG(2/0)
177 GGATG
BstYI 89 AGATCC [GA]^GATC[CT]
492 GGATCC
Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC
253 GCAAGC
EarI 73 CTCTTC CTCTTC(1/4)
211 GAAGAG
FokI 1020 CATCC GGATG(9/13)
177 GGATG
Ksp632I 73 CTCTTC CTCTTC(1/4)
211 GAAGAG
MaeIII 558 GTTAC ^GT[ACGT]AC
757 GTTAC
MflI 89 AGATCC [GA]^GATC[CT]
492 GGATCC
MseI 479 TTAA T^TAA
734 TTAA
NlaIV 5 GGCTCC GG[ACGT]^[ACGT]CC
492 GGATCC
PshBI 478 ATTAAT AT^TAAT
733 ATTAAT
Tru9I 479 TTAA T^TAA
734 TTAA
XhoII 89 AGATCC [GA]^GATC[CT]
492 GGATCC
XmnI 739 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BmyI BsaJI BsmAI Bsp1286I BsrDI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HaeIII ItaI MaeI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI TaqI
TseI Tsp509I TthHB8I