(Circular) (Six-base) MAPSORT of: pFN21ASDA0448.seq Check: 4729 from: 1 to: 5797 pFN21ASDA0448 5797 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2712 2712 Size: 5797 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2805 3860 5244 5512 1632 Size: 1173 1055 1384 268 1917 Fragments arranged by size: 1917 1384 1173 1055 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2446 4696 5673 1203 Size: 1243 2250 977 1327 Fragments arranged by size: 2250 1327 1243 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 2168 4526 2168 Size: 2358 3439 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2951 2983 2951 Size: 32 5765 AlwNI CAG_nnn'CTG Cuts at: 2065 2122 3540 5112 2065 Size: 57 1418 1572 2750 Fragments arranged by size: 2750 1572 1418 57 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 2471 2975 3106 3332 3484 5593 1493 Size: 978 504 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 978 504 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 2502 2757 4351 160 Size: 872 1470 255 1594 1606 Fragments arranged by size: 1606 1594 1470 872 255 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 2515 3127 3494 2515 Size: 612 367 4818 Fragments arranged by size: 4818 612 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 3010 3116 1391 Size: 640 979 106 4072 Fragments arranged by size: 4072 979 640 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4826 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 2247 1548 Size: 699 5098 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 3858 5014 1790 Size: 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2637 4457 1239 Size: 905 493 1820 2579 Fragments arranged by size: 2579 1820 905 493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3027 4460 882 Size: 617 1528 1433 2219 Fragments arranged by size: 2219 1528 1433 617 BsaAI yAC'GTr Cuts at: 493 1204 3094 3588 493 Size: 711 1890 494 2702 Fragments arranged by size: 2702 1890 711 494 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2981 1178 Size: 30 165 30 1548 30 3994 Fragments arranged by size: 3994 1548 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2141 1186 Size: 835 120 4842 Fragments arranged by size: 4842 835 120 BseYI C'CCAG_C Cuts at: 5000 5496 5000 Size: 496 5301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2653 3858 3943 5014 2653 Size: 1205 85 1071 3436 Fragments arranged by size: 3436 1205 1071 85 BsmI GAATG_Cn' Cuts at: 2708 3308 3401 2708 Size: 600 93 5104 Fragments arranged by size: 5104 600 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2581 2581 Size: 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2653 3010 3116 3858 3943 5014 Size: 399 241 622 357 106 742 85 1071 Cuts at: 5014 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 622 399 357 241 106 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 3152 844 Size: 1868 440 3489 Fragments arranged by size: 3489 1868 440 BsrBI CCG'CTC Cuts at: 1394 1823 2136 3684 1394 Size: 429 313 1548 3507 Fragments arranged by size: 3507 1548 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 2372 2822 2922 4292 4466 65 Size: 1183 1124 450 100 1370 174 1396 Fragments arranged by size: 1396 1370 1183 1124 450 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 2007 4601 2007 Size: 2594 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2112 2515 3127 3494 3879 3896 5348 5434 Size: 140 403 612 367 385 17 1452 86 Cuts at: 5434 5446 5792 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 612 403 385 367 346 140 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2033 2033 Size: 5797 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4288 Fragments arranged by size: 4288 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2105 487 Size: 683 56 879 4179 Fragments arranged by size: 4179 879 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2689 3244 4133 Size: 54 1425 50 275 18 859 555 889 Cuts at: 4133 5762 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 859 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2229 3727 1353 Size: 749 127 1498 3423 Fragments arranged by size: 3423 1498 749 127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2637 2805 3860 4457 5244 5512 Size: 393 512 493 168 1055 597 787 268 Cuts at: 5512 1239 Size: 1524 Fragments arranged by size: 1524 1055 787 597 512 493 393 268 168 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2154 2186 4438 4470 4512 Size: 32 135 32 582 32 2252 32 42 Cuts at: 4512 4544 1373 Size: 32 2626 Fragments arranged by size: 2626 2252 582 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2691 10 Size: 54 1425 343 859 3116 Fragments arranged by size: 3116 1425 859 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4114 4273 518 Size: 648 2589 359 159 2042 Fragments arranged by size: 2589 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 3213 3890 5038 5283 1606 Size: 456 1151 677 1148 245 2120 Fragments arranged by size: 2120 1151 1148 677 456 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4288 Fragments arranged by size: 4288 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2122 1108 Size: 1014 4783 PfoI T'CCnGG_A Cuts at: 2411 2411 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2951 2983 3866 3898 5379 5411 2951 Size: 32 883 32 1481 32 3337 Fragments arranged by size: 3337 1481 883 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 2256 3367 2256 Size: 1111 4686 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2062 2062 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SapI GCTCTTCn'nnn_ Cuts at: 2102 2102 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2933 3145 4280 4961 5152 826 Size: 219 1888 212 1135 681 191 1471 Fragments arranged by size: 1888 1471 1135 681 219 212 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3901 4802 5064 820 Size: 197 926 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3094 3588 493 Size: 2601 494 2702 Fragments arranged by size: 2702 2601 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 2765 3721 5 Size: 47 2713 956 2081 Fragments arranged by size: 2713 2081 956 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2022 3236 513 Size: 552 957 1214 3074 Fragments arranged by size: 3074 1214 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2661 2661 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 2766 2966 3926 2766 Size: 200 960 4637 Fragments arranged by size: 4637 960 200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI