(Circular) (Six-base) MAPSORT of: pFN21ASDA0426.seq Check: 1448 from: 1 to: 6541 pFN21ASDA0426 6541 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4351 278 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2476 3884 1937 Size: 539 1408 4594 Fragments arranged by size: 4594 1408 539 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2152 2643 2730 3358 3691 4604 5988 6256 Size: 520 491 87 628 333 913 1384 268 Cuts at: 6256 1632 Size: 1917 Fragments arranged by size: 1917 1384 913 628 520 491 333 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 5440 6417 1203 Size: 4237 977 1327 Fragments arranged by size: 4237 1327 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 2196 2472 5270 2196 Size: 276 2798 3467 Fragments arranged by size: 3467 2798 276 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AlwNI CAG_nnn'CTG Cuts at: 2245 2278 2305 4284 5856 2245 Size: 33 27 1979 1572 2930 Fragments arranged by size: 2930 1979 1572 33 27 ApaLI G'TGCA_C Cuts at: 3184 4598 5754 3184 Size: 1414 1156 3971 Fragments arranged by size: 3971 1414 1156 ApoI r'AATT_y Cuts at: 1493 2602 2657 3405 3489 3850 4076 4228 6337 Size: 1109 55 748 84 361 226 152 2109 Cuts at: 6337 1493 Size: 1697 Fragments arranged by size: 2109 1697 1109 748 361 226 152 84 55 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 3866 1870 Size: 73 1923 4545 Fragments arranged by size: 4545 1923 73 BamHI G'GATC_C Cuts at: 2904 3871 4238 2904 Size: 967 367 5207 Fragments arranged by size: 5207 967 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3251 3862 5317 618 Size: 325 217 627 1464 611 1455 1842 Fragments arranged by size: 1842 1464 1455 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2328 2730 3794 3860 1391 Size: 937 402 1064 66 4072 Fragments arranged by size: 4072 1064 937 402 66 BbeI G_GCGC'C Cuts at: 3255 3255 Size: 6541 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 2651 3728 928 Size: 971 247 505 1077 3741 Fragments arranged by size: 3741 1077 971 505 247 BbvCI CC'TCA_GC Cuts at: 2246 2246 Size: 6541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2214 2248 4732 4766 1903 Size: 34 277 34 2484 34 3678 Fragments arranged by size: 3678 2484 277 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4433 5643 4433 Size: 1210 5331 BclI T'GATC_A Cuts at: 1548 1548 Size: 6541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5152 136 Size: 107 122 71 631 605 3480 1525 Fragments arranged by size: 3480 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3829 6536 3829 Size: 2707 3834 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 3188 4602 5758 1790 Size: 1398 1414 1156 2573 Fragments arranged by size: 2573 1414 1398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6541 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2448 2490 3423 Size: 416 53 83 399 24 1031 42 933 Cuts at: 3423 3675 4272 5230 442 Size: 252 597 958 1753 Fragments arranged by size: 1753 1031 958 933 597 416 399 252 83 53 42 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 6541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2312 2315 2405 2492 2526 2725 2934 Size: 1061 12 3 90 87 34 199 209 Cuts at: 2934 3018 3519 3603 3792 5201 1239 Size: 84 501 84 189 1409 2579 Fragments arranged by size: 2579 1409 1061 501 209 199 189 90 87 84 84 34 12 3 Bpu10I CC'TnA_GC Cuts at: 2246 3788 2246 Size: 1542 4999 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4666 5531 5829 4666 Size: 865 298 5378 Fragments arranged by size: 5378 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2249 2431 5204 882 Size: 617 750 182 2773 2219 Fragments arranged by size: 2773 2219 750 617 182 BsaAI yAC'GTr Cuts at: 493 1204 3838 4332 493 Size: 711 2634 494 2702 Fragments arranged by size: 2702 2634 711 494 BsaBI GATnn'nnATC Cuts at: 6535 6535 Size: 6541 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3252 4348 4730 5365 Size: 53 83 186 936 1719 1096 382 635 Cuts at: 5365 275 Size: 1451 Fragments arranged by size: 1719 1451 1096 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4974 5372 5646 5793 6204 4974 Size: 398 274 147 411 5311 Fragments arranged by size: 5311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2409 2439 1178 Size: 30 165 30 1006 30 5280 Fragments arranged by size: 5280 1006 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2381 1186 Size: 1150 45 5346 Fragments arranged by size: 5346 1150 45 BseYI C'CCAG_C Cuts at: 2523 5744 6240 2523 Size: 3221 496 2824 Fragments arranged by size: 3221 2824 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 1163 Size: 573 624 5344 Fragments arranged by size: 5344 624 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4752 5780 1540 Size: 275 186 1990 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3188 3794 4602 4687 5758 2328 Size: 860 606 808 85 1071 3111 Fragments arranged by size: 3111 1071 860 808 606 85 BsmI GAATG_Cn' Cuts at: 3622 4052 4145 3622 Size: 430 93 6018 Fragments arranged by size: 6018 430 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2459 2459 Size: 6541 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2730 3188 3794 3860 4602 4687 Size: 399 538 402 458 606 66 742 85 Cuts at: 4687 5758 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 606 538 458 402 399 85 66 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 4430 6160 4430 Size: 1730 4811 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2413 3896 844 Size: 1286 283 1483 3489 Fragments arranged by size: 3489 1483 1286 283 BsrBI CCG'CTC Cuts at: 1394 1823 4428 1394 Size: 429 2605 3507 Fragments arranged by size: 3507 2605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5036 5210 65 Size: 1183 3788 174 1396 Fragments arranged by size: 3788 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3042 3248 5185 5372 1700 Size: 110 1232 206 1937 187 2869 Fragments arranged by size: 2869 1937 1232 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 2700 4601 5613 2700 Size: 1901 1012 3628 Fragments arranged by size: 3628 1901 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6541 BstBI TT'CG_AA Cuts at: 2007 5345 2007 Size: 3338 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstYI r'GATC_y Cuts at: 1972 2904 3156 3829 3871 4238 4623 4640 6092 Size: 932 252 673 42 367 385 17 1452 Cuts at: 6092 6178 6190 6536 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 932 673 385 367 346 252 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2319 2319 Size: 6541 BtgI C'CryG_G Cuts at: 513 1065 2022 2956 513 Size: 552 957 934 4098 Fragments arranged by size: 4098 957 934 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 4057 4851 4871 4057 Size: 794 20 5727 Fragments arranged by size: 5727 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3259 3294 529 Size: 35 2695 35 3776 Fragments arranged by size: 3776 2695 35 35 DraI TTT'AAA Cuts at: 2607 3845 2607 Size: 1238 5303 DraIII CAC_nnn'GTG Cuts at: 3192 3192 Size: 6541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5548 809 Size: 507 4232 1802 Fragments arranged by size: 4232 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3040 3988 4877 Size: 54 1425 50 275 18 1210 948 889 Cuts at: 4877 6506 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1210 948 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2718 4471 1353 Size: 732 633 1753 3423 Fragments arranged by size: 3423 1753 732 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5128 5502 5648 387 Size: 1064 138 3539 374 146 1280 Fragments arranged by size: 3539 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2326 3792 2326 Size: 1466 5075 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2300 2312 2315 2405 2492 2526 Size: 393 520 148 12 3 90 87 34 Cuts at: 2526 2643 2725 2730 2934 3018 3358 3519 3603 Size: 117 82 5 204 84 340 161 84 Cuts at: 3603 3691 3792 4604 5201 5988 6256 1239 Size: 88 101 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 597 520 393 340 268 204 161 148 117 101 90 88 87 84 84 82 34 12 5 3 EcoNI CCTnn'n_nnAGG Cuts at: 2318 2318 Size: 6541 EcoO109I rG'GnC_Cy Cuts at: 2119 3591 2119 Size: 1472 5069 EcoRI G'AATT_C Cuts at: 3405 3489 3850 4228 3405 Size: 84 361 378 5718 Fragments arranged by size: 5718 378 361 84 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 3255 5688 1678 Size: 1577 2433 2531 Fragments arranged by size: 2531 2433 1577 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 2409 2441 2458 Size: 32 135 32 610 32 195 32 17 Cuts at: 2458 2490 2809 2841 3540 3572 5182 5214 5256 Size: 32 319 32 699 32 1610 32 42 Cuts at: 5256 5288 1373 Size: 32 2626 Fragments arranged by size: 2626 1610 699 610 319 195 135 42 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 3885 4131 669 Size: 988 281 1947 246 3079 Fragments arranged by size: 3079 1947 988 281 246 HindIII A'AGCT_T Cuts at: 748 2865 2949 748 Size: 2117 84 4340 Fragments arranged by size: 4340 2117 84 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KasI G'GCGC_C Cuts at: 3251 3251 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 4140 4140 Size: 6541 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5655 5839 1305 Size: 4350 184 2007 Fragments arranged by size: 4350 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4719 Fragments arranged by size: 4719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3102 4499 4858 5017 518 Size: 648 1936 1397 359 159 2042 Fragments arranged by size: 2042 1936 1397 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3957 4634 5782 6027 1606 Size: 2351 677 1148 245 2120 Fragments arranged by size: 2351 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3252 3252 Size: 6541 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5032 Fragments arranged by size: 5032 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 3899 5444 1132 Size: 97 2670 1545 2229 Fragments arranged by size: 2670 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 5440 5440 Size: 6541 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 1108 Size: 1252 5289 PfoI T'CCnGG_A Cuts at: 2827 2827 Size: 6541 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4610 4642 6123 6155 4610 Size: 32 1481 32 4996 Fragments arranged by size: 4996 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2119 Size: 6541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 3297 4111 3297 Size: 814 5727 PstI C_TGCA'G Cuts at: 830 3893 830 Size: 3063 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 SacI G_AGCT'C Cuts at: 2328 3794 2328 Size: 1466 5075 SalI G'TCGA_C Cuts at: 1936 3883 1936 Size: 1947 4594 SapI GCTCTTCn'nnn_ Cuts at: 2718 2718 Size: 6541 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 2165 4789 6501 2165 Size: 2624 1712 2205 Fragments arranged by size: 2624 2205 1712 SfcI C'TryA_G Cuts at: 826 1045 3889 5024 5705 5896 826 Size: 219 2844 1135 681 191 1471 Fragments arranged by size: 2844 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3253 3253 Size: 6541 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4645 5546 5808 820 Size: 197 926 2702 901 262 1553 Fragments arranged by size: 2702 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3838 4332 493 Size: 3345 494 2702 Fragments arranged by size: 3345 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3899 1132 Size: 2767 3774 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 2333 3138 1517 Size: 816 805 4920 Fragments arranged by size: 4920 816 805 StyI C'CwwG_G Cuts at: 513 1065 2022 3980 513 Size: 552 957 1958 3074 Fragments arranged by size: 3074 1958 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2326 4568 4585 4738 4923 5368 Size: 1255 573 173 2242 17 153 185 445 Cuts at: 5368 325 Size: 1498 Fragments arranged by size: 2242 1498 1255 573 445 185 173 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 4787 6499 Size: 274 80 33 51 1443 186 2624 1712 Cuts at: 6499 96 Size: 138 Fragments arranged by size: 2624 1712 1443 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6541 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2192 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 2274 3371 3620 3741 4670 2274 Size: 1097 249 121 929 4145 Fragments arranged by size: 4145 1097 929 249 121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4349 276 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI