Restriction map for pFN21ASDA0398
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HhaI        
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI          714  GCGG                 CCGC(-3/-1)
AcyI          977  GACGCC               G[GA]^CG[CT]C
AflII        1365  CTTAAG               C^TTAAG
AlwI          253  GATCC                GGATC(4/5)
AseI           94  ATTAAT               AT^TAAT
AsnI           94  ATTAAT               AT^TAAT
AvaII         606  GGACC                G^G[AT]CC
BbsI           81  GTCTTC               GAAGAC(2/6)
BfrI         1365  CTTAAG               C^TTAAG
BglI          926  GCCCTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          356  CTGGAG               CTGGAG(16/14)
BpuAI          81  GTCTTC               GAAGAC(2/6)
BsaHI         977  GACGCC               G[GA]^CG[CT]C
BsgI          761  GTGCAG               GTGCAG(16/14)
BsiYI        1054  CCTTTATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1054  CCTTTATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI         1241  GAATGC               GAATGC(1/-1)
BsrDI        1127  CATTGC               GCAATG(2/0)
BstAPI          4  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1349  GGTTACC              G^GT[ACGT]ACC
BstPI        1349  GGTTACC              G^GT[ACGT]ACC
Cfr13I        606  GGACC                G^G[ACGT]CC
EarI          240  GAAGAG               CTCTTC(1/4)
EcoO65I      1349  GGTTACC              G^GT[ACGT]ACC
EcoRV         592  GATATC               GAT^ATC
EcoT14I      1250  CCTTGG               C^C[AT][AT]GG
HaeIII       1248  GGCC                 GG^CC
Hin1I         977  GACGCC               G[GA]^CG[CT]C
HindIII       986  AAGCTT               A^AGCTT
HphI          286  GGTGA                GGTGA(8/7)
Ksp632I       240  GAAGAG               CTCTTC(1/4)
MaeII         588  ACGT                 A^CGT
MspA1I        712  CAGCGG               C[AC]G^C[GT]G
NspI         1314  ACATGC               [GA]CATG^[CT]
PshAI         138  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI          94  ATTAAT               AT^TAAT
PstI          840  CTGCAG               CTGCA^G
Sau96I        606  GGACC                G^G[ACGT]CC
SpeI         1164  ACTAGT               A^CTAGT
SspI           91  AATATT               AAT^ATT
StuI         1247  AGGCCT               AGG^CCT
StyI         1250  CCTTGG               C^C[AT][AT]GG
TfiI         1000  GAATC                G^A[AT]TC
Tsp45I        815  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          157  GTAGAC               GT^[AC][GT]AC
             1168  GTCTAC              
AlwNI         110  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              443  CAGTGGCTG           
BsmFI         118  GGGAC                GGGAC(10/14)
              265  GTCCC               
BsrI          121  ACTGG                ACTGG(1/-1)
              355  ACTGG               
BssKI         609  CCTGG                ^CC[ACGT]GG
              928  CCTGG               
BstNI         609  CCTGG                CC^[AT]GG
              928  CCTGG               
Cac8I         885  GCAGGC               GC[ACGT]^[ACGT]GC
             1245  GCAGGC              
DpnII         193  GATC                 ^GATC
              253  GATC                
EcoRII        609  CCTGG                ^CC[AT]GG
              928  CCTGG               
HgaI          977  GACGC                GACGC(5/10)
               58  GCGTC               
MaeIII        815  GTGAC                ^GT[ACGT]AC
             1350  GTTAC               
MboI          193  GATC                 ^GATC
              253  GATC                
MslI          454  CATTACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              821  CACAAATGTG          
MvaI          609  CCTGG                CC^[AT]GG
              928  CCTGG               
MwoI            4  GCAAATTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              926  GCCCTGGGGGC         
NdeII         193  GATC                 ^GATC
              253  GATC                
NlaIV        1254  GGAGCC               GG[ACGT]^[ACGT]CC
             1360  GGAACC              
PleI           79  GAGTC                GAGTC(4/5)
              874  GAGTC               
Sau3AI        193  GATC                 ^GATC
              253  GATC                
ScrFI         609  CCTGG                CC^[ACGT]GG
              928  CCTGG               
SfaNI         289  GATGC                GCATC(5/9)
              897  GATGC               
SfcI          147  CTGTAG               C^T[GA][CT]AG
              840  CTGCAG              
SmlI          391  CTTGAG               C^T[CT][GA]AG
             1365  CTTAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BfaI        BsaJI       BsmAI       BstF5I      DdeI        
EcoRI       Fnu4HI      FokI        HinfI       ItaI        
MaeI        MboII       MnlI        MseI        NlaIII      
RsaI        Tru9I       TseI        Tsp509I     TspRI       
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