Restriction map for
pFN21ASDA0398
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 714 GCGG CCGC(-3/-1)
AcyI 977 GACGCC G[GA]^CG[CT]C
AflII 1365 CTTAAG C^TTAAG
AlwI 253 GATCC GGATC(4/5)
AseI 94 ATTAAT AT^TAAT
AsnI 94 ATTAAT AT^TAAT
AvaII 606 GGACC G^G[AT]CC
BbsI 81 GTCTTC GAAGAC(2/6)
BfrI 1365 CTTAAG C^TTAAG
BglI 926 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 356 CTGGAG CTGGAG(16/14)
BpuAI 81 GTCTTC GAAGAC(2/6)
BsaHI 977 GACGCC G[GA]^CG[CT]C
BsgI 761 GTGCAG GTGCAG(16/14)
BsiYI 1054 CCTTTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1054 CCTTTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1241 GAATGC GAATGC(1/-1)
BsrDI 1127 CATTGC GCAATG(2/0)
BstAPI 4 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1349 GGTTACC G^GT[ACGT]ACC
BstPI 1349 GGTTACC G^GT[ACGT]ACC
Cfr13I 606 GGACC G^G[ACGT]CC
EarI 240 GAAGAG CTCTTC(1/4)
EcoO65I 1349 GGTTACC G^GT[ACGT]ACC
EcoRV 592 GATATC GAT^ATC
EcoT14I 1250 CCTTGG C^C[AT][AT]GG
HaeIII 1248 GGCC GG^CC
Hin1I 977 GACGCC G[GA]^CG[CT]C
HindIII 986 AAGCTT A^AGCTT
HphI 286 GGTGA GGTGA(8/7)
Ksp632I 240 GAAGAG CTCTTC(1/4)
MaeII 588 ACGT A^CGT
MspA1I 712 CAGCGG C[AC]G^C[GT]G
NspI 1314 ACATGC [GA]CATG^[CT]
PshAI 138 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 94 ATTAAT AT^TAAT
PstI 840 CTGCAG CTGCA^G
Sau96I 606 GGACC G^G[ACGT]CC
SpeI 1164 ACTAGT A^CTAGT
SspI 91 AATATT AAT^ATT
StuI 1247 AGGCCT AGG^CCT
StyI 1250 CCTTGG C^C[AT][AT]GG
TfiI 1000 GAATC G^A[AT]TC
Tsp45I 815 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 157 GTAGAC GT^[AC][GT]AC
1168 GTCTAC
AlwNI 110 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
443 CAGTGGCTG
BsmFI 118 GGGAC GGGAC(10/14)
265 GTCCC
BsrI 121 ACTGG ACTGG(1/-1)
355 ACTGG
BssKI 609 CCTGG ^CC[ACGT]GG
928 CCTGG
BstNI 609 CCTGG CC^[AT]GG
928 CCTGG
Cac8I 885 GCAGGC GC[ACGT]^[ACGT]GC
1245 GCAGGC
DpnII 193 GATC ^GATC
253 GATC
EcoRII 609 CCTGG ^CC[AT]GG
928 CCTGG
HgaI 977 GACGC GACGC(5/10)
58 GCGTC
MaeIII 815 GTGAC ^GT[ACGT]AC
1350 GTTAC
MboI 193 GATC ^GATC
253 GATC
MslI 454 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
821 CACAAATGTG
MvaI 609 CCTGG CC^[AT]GG
928 CCTGG
MwoI 4 GCAAATTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
926 GCCCTGGGGGC
NdeII 193 GATC ^GATC
253 GATC
NlaIV 1254 GGAGCC GG[ACGT]^[ACGT]CC
1360 GGAACC
PleI 79 GAGTC GAGTC(4/5)
874 GAGTC
Sau3AI 193 GATC ^GATC
253 GATC
ScrFI 609 CCTGG CC^[ACGT]GG
928 CCTGG
SfaNI 289 GATGC GCATC(5/9)
897 GATGC
SfcI 147 CTGTAG C^T[GA][CT]AG
840 CTGCAG
SmlI 391 CTTGAG C^T[CT][GA]AG
1365 CTTAAG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
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AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsmAI BstF5I DdeI
EcoRI Fnu4HI FokI HinfI ItaI
MaeI MboII MnlI MseI NlaIII
RsaI Tru9I TseI Tsp509I TspRI