(Circular) (Six-base) MAPSORT of: pFN21ASDA0398.seq Check: 7498 from: 1 to: 6157 pFN21ASDA0398 6157 bp With 182 enzymes: SgfI * February 10, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3967 278 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2181 3192 3500 1937 Size: 244 1011 308 4594 Fragments arranged by size: 4594 1011 308 244 Acc65I G'GTAC_C Cuts at: 3478 3478 Size: 6157 AclI AA'CG_TT Cuts at: 1563 4284 4657 1563 Size: 2721 373 3063 Fragments arranged by size: 3063 2721 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 2474 2997 4220 5604 5872 1632 Size: 705 137 523 1223 1384 268 1917 Fragments arranged by size: 1917 1384 1223 705 523 268 137 AflII C'TTAA_G Cuts at: 820 1017 3388 820 Size: 197 2371 3589 Fragments arranged by size: 3589 2371 197 AflIII A'CryG_T Cuts at: 1203 5056 6033 1203 Size: 3853 977 1327 Fragments arranged by size: 3853 1327 977 AgeI A'CCGG_T Cuts at: 4988 4988 Size: 6157 AhdI GACnn_n'nnGTC Cuts at: 4886 4886 Size: 6157 AleI CACnn'nnGTG Cuts at: 1166 2848 1166 Size: 1682 4475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6157 AlwNI CAG_nnn'CTG Cuts at: 2138 2471 3900 5472 2138 Size: 333 1429 1572 2823 Fragments arranged by size: 2823 1572 1429 333 ApaLI G'TGCA_C Cuts at: 4214 5370 4214 Size: 1156 5001 ApoI r'AATT_y Cuts at: 1493 2029 2089 2579 2789 2831 3053 3131 3212 Size: 536 60 490 210 42 222 78 81 Cuts at: 3212 3466 3692 3844 5953 1493 Size: 254 226 152 2109 1697 Fragments arranged by size: 2109 1697 536 490 254 226 222 210 152 81 78 60 42 AseI AT'TA_AT Cuts at: 160 1032 2118 4711 160 Size: 872 1086 2593 1606 Fragments arranged by size: 2593 1606 1086 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 AvaI C'yCGr_G Cuts at: 1870 1943 3482 1870 Size: 73 1539 4545 Fragments arranged by size: 4545 1539 73 BamHI G'GATC_C Cuts at: 3487 3854 3487 Size: 367 5790 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3478 4933 618 Size: 325 217 627 1691 1455 1842 Fragments arranged by size: 1842 1691 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3476 1391 Size: 2085 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 928 Size: 971 198 4988 Fragments arranged by size: 4988 971 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4348 4382 1903 Size: 34 2411 34 3678 Fragments arranged by size: 3678 2411 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4049 5259 4049 Size: 1210 4947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2955 4768 136 Size: 107 122 71 631 605 1283 1813 1525 Fragments arranged by size: 1813 1525 1283 631 605 122 107 71 BglII A'GATC_T Cuts at: 6152 6152 Size: 6157 BlpI GC'TnA_GC Cuts at: 3574 3574 Size: 6157 Bme1580I G_kGCm'C Cuts at: 1790 4218 5374 1790 Size: 2428 1156 2573 Fragments arranged by size: 2573 2428 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6157 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2154 3888 4846 Size: 416 53 83 399 24 737 1734 958 Cuts at: 4846 442 Size: 1753 Fragments arranged by size: 1753 1734 958 737 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 4817 1239 Size: 1161 2417 2579 Fragments arranged by size: 2579 2417 1161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 4282 5147 5445 2435 Size: 1847 865 298 3147 Fragments arranged by size: 3147 1847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4820 882 Size: 617 3321 2219 Fragments arranged by size: 3321 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3454 3948 493 Size: 711 2250 494 2702 Fragments arranged by size: 2702 2250 711 494 BsaBI GATnn'nnATC Cuts at: 6151 6151 Size: 6157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3001 3964 4346 4981 Size: 53 83 186 936 1468 963 382 635 Cuts at: 4981 275 Size: 1451 Fragments arranged by size: 1468 1451 963 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4590 4988 5262 5409 5820 4590 Size: 398 274 147 411 4927 Fragments arranged by size: 4927 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5932 Fragments arranged by size: 5932 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6157 BseYI C'CCAG_C Cuts at: 5360 5856 5360 Size: 496 5661 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2805 1163 Size: 573 1069 4515 Fragments arranged by size: 4515 1069 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3607 4368 5396 1540 Size: 275 186 1606 761 1028 2301 Fragments arranged by size: 2301 1606 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4218 4303 5374 4218 Size: 85 1071 5001 Fragments arranged by size: 5001 1071 85 BsmI GAATG_Cn' Cuts at: 3270 3668 3761 3270 Size: 398 93 5666 Fragments arranged by size: 5666 398 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3476 4218 4303 5374 1391 Size: 399 1686 742 85 1071 2174 Fragments arranged by size: 2174 1686 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5820 5820 Size: 6157 BspHI T'CATG_A Cuts at: 4046 5776 4046 Size: 1730 4427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3512 844 Size: 2668 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4044 1394 Size: 429 2221 3507 Fragments arranged by size: 3507 2221 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3149 4652 4826 65 Size: 1183 1901 1503 174 1396 Fragments arranged by size: 1901 1503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4801 4988 1700 Size: 110 2991 187 2869 Fragments arranged by size: 2991 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6157 BssSI C'ACGA_G Cuts at: 4217 5229 4217 Size: 1012 5145 BstAPI GCAn_nnn'nTGC Cuts at: 794 2033 794 Size: 1239 4918 BstBI TT'CG_AA Cuts at: 2007 4961 2007 Size: 2954 3203 BstEII G'GTnAC_C Cuts at: 1184 3372 1184 Size: 2188 3969 BstYI r'GATC_y Cuts at: 1972 3487 3854 4239 4256 5708 5794 5806 6152 Size: 1515 367 385 17 1452 86 12 346 Cuts at: 6152 1972 Size: 1977 Fragments arranged by size: 1977 1515 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4648 Fragments arranged by size: 4648 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5418 Fragments arranged by size: 5418 683 56 BtsI GCAGTG_nn' Cuts at: 3673 4467 4487 3673 Size: 794 20 5343 Fragments arranged by size: 5343 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6157 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6122 DraI TTT'AAA Cuts at: 3461 3461 Size: 6157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5164 809 Size: 507 3848 1802 Fragments arranged by size: 3848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3604 4493 6122 Size: 54 1425 50 275 18 1774 889 1629 Cuts at: 6122 8 Size: 43 Fragments arranged by size: 1774 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3604 1537 Size: 275 1792 4090 Fragments arranged by size: 4090 1792 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 4087 1353 Size: 905 1829 3423 Fragments arranged by size: 3423 1829 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4744 5118 5264 387 Size: 1064 138 3155 374 146 1280 Fragments arranged by size: 3155 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2400 2474 2997 4220 4817 5604 Size: 393 705 63 74 523 1223 597 787 Cuts at: 5604 5872 1239 Size: 268 1524 Fragments arranged by size: 1524 1223 787 705 597 523 393 268 74 63 EcoRI G'AATT_C Cuts at: 2089 3131 3212 3466 3844 2089 Size: 1042 81 254 378 4402 Fragments arranged by size: 4402 1042 378 254 81 EcoRV GAT'ATC Cuts at: 2617 2617 Size: 6157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2234 3393 2234 Size: 1159 4998 FspI TGC'GCA Cuts at: 4663 4663 Size: 6157 HaeII r_GCGC'y Cuts at: 1678 5304 1678 Size: 3626 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 4798 4830 4872 Size: 32 135 32 668 32 2526 32 42 Cuts at: 4872 4904 1373 Size: 32 2626 Fragments arranged by size: 2626 2526 668 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3501 3747 669 Size: 988 281 1563 246 3079 Fragments arranged by size: 3079 1563 988 281 246 HindIII A'AGCT_T Cuts at: 748 3009 748 Size: 2261 3896 HpaI GTT'AAC Cuts at: 3747 3747 Size: 6157 KpnI G_GTAC'C Cuts at: 3482 3482 Size: 6157 MfeI C'AATT_G Cuts at: 3756 3756 Size: 6157 MluI A'CGCG_T Cuts at: 6033 6033 Size: 6157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5271 5455 1305 Size: 3966 184 2007 Fragments arranged by size: 3966 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4335 Fragments arranged by size: 4335 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 2848 4115 4474 4633 518 Size: 648 1315 367 1267 359 159 2042 Fragments arranged by size: 2042 1315 1267 648 367 359 159 MspA1I CmG'CkG Cuts at: 1606 2737 3573 4250 5398 5643 1606 Size: 1131 836 677 1148 245 2120 Fragments arranged by size: 2120 1148 1131 836 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4648 Fragments arranged by size: 4648 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6157 NotI GC'GGCC_GC Cuts at: 3604 3604 Size: 6157 NspI r_CATG'y Cuts at: 1132 1229 3341 3515 5060 1132 Size: 97 2112 174 1545 2229 Fragments arranged by size: 2229 2112 1545 174 97 PasI CC'CwG_GG Cuts at: 1348 2951 1348 Size: 1603 4554 PciI A'CATG_T Cuts at: 5056 5056 Size: 6157 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6157 PmeI GTTT'AAAC Cuts at: 3461 3461 Size: 6157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4226 4258 5739 5771 4226 Size: 32 1481 32 4612 Fragments arranged by size: 4612 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2165 1613 Size: 552 5605 PsiI TTA'TAA Cuts at: 2795 3727 2795 Size: 932 5225 PstI C_TGCA'G Cuts at: 830 2867 3509 830 Size: 2037 642 3478 Fragments arranged by size: 3478 2037 642 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6157 SalI G'TCGA_C Cuts at: 1936 3499 1936 Size: 1563 4594 SbfI CC_TGCA'GG Cuts at: 3509 3509 Size: 6157 ScaI AGT'ACT Cuts at: 4405 6117 4405 Size: 1712 4445 SfcI C'TryA_G Cuts at: 826 1045 2170 2863 3505 4640 5321 5512 826 Size: 219 1125 693 642 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 693 681 642 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6157 SmaI CCC'GGG Cuts at: 1872 3484 1872 Size: 1612 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 3388 4261 5162 5424 820 Size: 197 926 471 974 873 901 262 1553 Fragments arranged by size: 1553 974 926 901 873 471 262 197 SnaBI TAC'GTA Cuts at: 493 3454 3948 493 Size: 2961 494 2702 Fragments arranged by size: 2961 2702 494 SpeI A'CTAG_T Cuts at: 152 3187 152 Size: 3035 3122 SphI G_CATG'C Cuts at: 1132 3515 1132 Size: 2383 3774 SspI AAT'ATT Cuts at: 5 52 2116 4081 5 Size: 47 2064 1965 2081 Fragments arranged by size: 2081 2064 1965 47 StuI AGG'CCT Cuts at: 1517 3272 1517 Size: 1755 4402 StyI C'CwwG_G Cuts at: 513 1065 2022 3273 3596 513 Size: 552 957 1251 323 3074 Fragments arranged by size: 3074 1251 957 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4184 4201 4354 4539 4984 325 Size: 1255 2604 17 153 185 445 1498 Fragments arranged by size: 2604 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2696 3174 3348 Size: 274 80 33 51 1443 719 478 174 Cuts at: 3348 4403 6115 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 719 478 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2548 2580 6117 6149 2548 Size: 32 3537 32 2556 Fragments arranged by size: 3537 2556 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6157 XbaI T'CTAG_A Cuts at: 3493 3493 Size: 6157 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6157 XmaI C'CCGG_G Cuts at: 1870 3482 1870 Size: 1612 4545 XmnI GAAnn'nnTTC Cuts at: 4286 4286 Size: 6157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3965 276 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI