Restriction map for
pFN21ASDA0387
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbeI
BciVI BclI BfrI BlnI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI EclXI
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI MseI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI Tru9I
Tth111I XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1663 GACGTC GACGT^C
AcsI 1832 AAATTC [GA]^AATT[CT]
AhdI 1282 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 878 GTGCAC G^TGCAC
Aor51HI 2319 AGCGCT AGC^GCT
ApaLI 878 GTGCAC G^TGCAC
ApoI 1832 AAATTC [GA]^AATT[CT]
Asp700I 57 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1282 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 351 CACGTG CAC^GTG
BglII 1431 AGATCT A^GATCT
BsaAI 351 CACGTG [CT]AC^GT[GA]
BsaBI 2982 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1843 ACCGGA [AT]^CCGG[AT]
BssSI 706 CACGAG CACGAG(-5/-1)
BstAPI 306 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1494 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1431 AGATCT [GA]^GATC[CT]
DrdI 2806 GACCGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1282 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2319 AGCGCT AGC^GCT
EcoRV 2986 GATATC GAT^ATC
HincII 1792 GTTGAC GT[CT]^[GA]AC
HindII 1792 GTTGAC GT[CT]^[GA]AC
HindIII 331 AAGCTT A^AGCTT
MamI 2982 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1567 CAATTG C^AATTG
MflI 1431 AGATCT [GA]^GATC[CT]
MunI 1567 CAATTG C^AATTG
NaeI 1020 GCCGGC GCC^GGC
NcoI 1101 CCATGG C^CATGG
NgoMIV 1020 GCCGGC G^CCGGC
NheI 2476 GCTAGC G^CTAGC
PmaCI 351 CACGTG CAC^GTG
PmlI 351 CACGTG CAC^GTG
SexAI 2690 ACCTGGT A^CC[AT]GGT
SfiI 1036 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 17 AATATT AAT^ATT
XhoII 1431 AGATCT [GA]^GATC[CT]
XmnI 57 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 662 ACACGT A^C[GA][CT]GT
2126 ACACGT
BcgI 2037 CGACCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1691 GCAGCGCGTTCG
BlpI 753 GCTCAGC GC^T[ACGT]AGC
1066 GCTGAGC
Bpu1102I 753 GCTCAGC GC^T[ACGT]AGC
1066 GCTGAGC
BsmBI 2145 CGTCTC CGTCTC(1/5)
2764 GAGACG
BsmI 890 GAATGC GAATGC(1/-1)
1452 GCATTC
BsrDI 2899 CATTGC GCAATG(2/0)
265 GCAATG
CelII 753 GCTCAGC GC^T[ACGT]AGC
1066 GCTGAGC
EarI 1381 GAAGAG CTCTTC(1/4)
1591 GAAGAG
EcoT14I 1101 CCATGG C^C[AT][AT]GG
1169 CCAAGG
Ksp632I 1381 GAAGAG CTCTTC(1/4)
1591 GAAGAG
MslI 2674 CACATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2899 CATTGCAGTG
PaeR7I 190 CTCGAG C^TCGAG
1112 CTCGAG
PflMI 789 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2082 CCAGCGTATGG
SacI 751 GAGCTC GAGCT^C
1483 GAGCTC
SbfI 1275 CCTGCAGG CCTGCA^GG
1677 CCTGCAGG
Sse8387I 1275 CCTGCAGG CCTGCA^GG
1677 CCTGCAGG
StuI 218 AGGCCT AGG^CCT
898 AGGCCT
StyI 1101 CCATGG C^C[AT][AT]GG
1169 CCAAGG
Van91I 789 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2082 CCAGCGTATGG
XhoI 190 CTCGAG C^TCGAG
1112 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI