(Circular) (Six-base) MAPSORT of: pFN21ASDA0378.seq Check: 4508 from: 1 to: 7600 pFN21ASDA0378 7600 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5410 278 Size: 53 83 186 936 3874 2468 Fragments arranged by size: 3874 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4943 1937 Size: 3006 4594 Acc65I G'GTAC_C Cuts at: 4921 4921 Size: 7600 AclI AA'CG_TT Cuts at: 1563 5727 6100 1563 Size: 4164 373 3063 Fragments arranged by size: 4164 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 3104 3359 3470 3638 5663 7047 7315 Size: 824 648 255 111 168 2025 1384 268 Cuts at: 7315 1632 Size: 1917 Fragments arranged by size: 2025 1917 1384 824 648 268 255 168 111 AfeI AGC'GCT Cuts at: 3818 3818 Size: 7600 AflII C'TTAA_G Cuts at: 820 1017 2591 820 Size: 197 1574 5829 Fragments arranged by size: 5829 1574 197 AflIII A'CryG_T Cuts at: 1203 6499 7476 1203 Size: 5296 977 1327 Fragments arranged by size: 5296 1327 977 AgeI A'CCGG_T Cuts at: 4203 6431 4203 Size: 2228 5372 AhdI GACnn_n'nnGTC Cuts at: 6329 6329 Size: 7600 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7600 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7600 AlwNI CAG_nnn'CTG Cuts at: 3026 3764 4468 4510 5343 6915 3026 Size: 738 704 42 833 1572 3711 Fragments arranged by size: 3711 1572 833 738 704 42 ApaLI G'TGCA_C Cuts at: 5657 6813 5657 Size: 1156 6444 ApoI r'AATT_y Cuts at: 1493 3412 3600 4909 5135 5287 7396 1493 Size: 1919 188 1309 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 1309 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 6154 160 Size: 872 5122 1606 Fragments arranged by size: 5122 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7600 AvaI C'yCGr_G Cuts at: 1870 1943 2922 4925 1870 Size: 73 979 2003 4545 Fragments arranged by size: 4545 2003 979 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2192 2225 2192 Size: 33 7567 BamHI G'GATC_C Cuts at: 4930 5297 4930 Size: 367 7233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4921 6376 618 Size: 325 217 627 3134 1455 1842 Fragments arranged by size: 3134 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4919 1391 Size: 3528 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2873 3326 4398 4460 928 Size: 971 974 453 1072 62 4068 Fragments arranged by size: 4068 1072 974 971 453 62 BbvCI CC'TCA_GC Cuts at: 3401 3401 Size: 7600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5791 5825 1903 Size: 34 3854 34 3678 Fragments arranged by size: 3854 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5492 6702 5492 Size: 1210 6390 BclI T'GATC_A Cuts at: 1548 2218 3331 1548 Size: 670 1113 5817 Fragments arranged by size: 5817 1113 670 BfrBI ATG'CAT Cuts at: 3971 3971 Size: 7600 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6211 136 Size: 107 122 71 631 605 4539 1525 Fragments arranged by size: 4539 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2467 7595 2467 Size: 5128 2472 BlpI GC'TnA_GC Cuts at: 5017 5017 Size: 7600 Bme1580I G_kGCm'C Cuts at: 1790 2258 5661 6817 1790 Size: 468 3403 1156 2573 Fragments arranged by size: 3403 2573 1156 468 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5331 6289 442 Size: 416 53 83 399 24 3914 958 1753 Fragments arranged by size: 3914 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4299 4299 Size: 7600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2599 2732 3008 3134 4090 4232 4292 4469 Size: 1360 133 276 126 956 142 60 177 Cuts at: 4469 4586 4598 6260 1239 Size: 117 12 1662 2579 Fragments arranged by size: 2579 1662 1360 956 276 177 142 133 126 117 60 12 Bpu10I CC'TnA_GC Cuts at: 2586 2844 3401 2586 Size: 258 557 6785 Fragments arranged by size: 6785 557 258 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 2629 2969 3233 4205 4310 4676 5725 6590 Size: 2 340 264 972 105 366 1049 865 Cuts at: 6590 6888 2627 Size: 298 3339 Fragments arranged by size: 3339 1049 972 865 366 340 298 264 105 2 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2097 2724 6263 882 Size: 617 598 627 3539 2219 Fragments arranged by size: 3539 2219 627 617 598 BsaAI yAC'GTr Cuts at: 493 1204 4897 5391 493 Size: 711 3693 494 2702 Fragments arranged by size: 3693 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4779 7594 4779 Size: 2815 4785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4157 5407 5789 6424 Size: 53 83 186 936 2624 1250 382 635 Cuts at: 6424 275 Size: 1451 Fragments arranged by size: 2624 1451 1250 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 4203 4542 4861 6033 6431 6705 6852 7263 Size: 1689 339 319 1172 398 274 147 411 Cuts at: 7263 2514 Size: 2851 Fragments arranged by size: 2851 1689 1172 411 398 339 319 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 1178 Size: 30 165 30 886 30 6459 Fragments arranged by size: 6459 886 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2501 2735 2784 2816 4567 4583 4892 Size: 899 416 234 49 32 1751 16 309 Cuts at: 4892 1186 Size: 3894 Fragments arranged by size: 3894 1751 899 416 309 234 49 32 16 BseYI C'CCAG_C Cuts at: 2478 6803 7299 2478 Size: 4325 496 2779 Fragments arranged by size: 4325 2779 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3750 1163 Size: 573 2014 5013 Fragments arranged by size: 5013 2014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5050 5811 6839 1540 Size: 275 186 3049 761 1028 2301 Fragments arranged by size: 3049 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2781 3444 5661 5746 6817 2781 Size: 663 2217 85 1071 3564 Fragments arranged by size: 3564 2217 1071 663 85 BsmI GAATG_Cn' Cuts at: 4447 5111 5204 4447 Size: 664 93 6843 Fragments arranged by size: 6843 664 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2258 2781 3444 4919 5661 5746 6817 Size: 399 468 523 663 1475 742 85 1071 Cuts at: 6817 1391 Size: 2174 Fragments arranged by size: 2174 1475 1071 742 663 523 468 399 85 BspEI T'CCGG_A Cuts at: 2514 4542 4861 7263 2514 Size: 2028 319 2402 2851 Fragments arranged by size: 2851 2402 2028 319 BspHI T'CATG_A Cuts at: 2221 5489 7219 2221 Size: 3268 1730 2602 Fragments arranged by size: 3268 2602 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 4955 844 Size: 1590 2521 3489 Fragments arranged by size: 3489 2521 1590 BsrBI CCG'CTC Cuts at: 1394 1823 2774 5487 1394 Size: 429 951 2713 3507 Fragments arranged by size: 3507 2713 951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4629 6095 6269 65 Size: 1183 3381 1466 174 1396 Fragments arranged by size: 3381 1466 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3593 4203 6244 6431 1700 Size: 110 1783 610 2041 187 2869 Fragments arranged by size: 2869 2041 1783 610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7600 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7600 BssSI C'ACGA_G Cuts at: 5660 6672 5660 Size: 1012 6588 BstAPI GCAn_nnn'nTGC Cuts at: 794 2189 4601 4631 794 Size: 1395 2412 30 3763 Fragments arranged by size: 3763 2412 1395 30 BstBI TT'CG_AA Cuts at: 2007 6404 2007 Size: 4397 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7600 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 7600 BstYI r'GATC_y Cuts at: 1972 2072 2467 2549 3208 3222 4215 4572 4930 Size: 100 395 82 659 14 993 357 358 Cuts at: 4930 5297 5682 5699 7151 7237 7249 7595 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 993 659 395 385 367 358 357 346 100 86 82 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2313 513 Size: 552 1248 5800 Fragments arranged by size: 5800 1248 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6861 Fragments arranged by size: 6861 683 56 BtsI GCAGTG_nn' Cuts at: 5116 5910 5930 5116 Size: 794 20 6786 Fragments arranged by size: 6786 794 20 ClaI AT'CG_AT Cuts at: 1554 4819 1554 Size: 3265 4335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7565 DraI TTT'AAA Cuts at: 4904 4904 Size: 7600 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6607 809 Size: 507 5291 1802 Fragments arranged by size: 5291 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3591 4317 4512 Size: 54 1425 50 275 18 1761 726 195 Cuts at: 4512 4533 5047 5936 7565 8 Size: 21 514 889 1629 43 Fragments arranged by size: 1761 1629 1425 889 726 514 275 195 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 5047 1537 Size: 275 3235 4090 Fragments arranged by size: 4090 3235 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2613 3267 3570 3855 5530 1353 Size: 1260 654 303 285 1675 3423 Fragments arranged by size: 3423 1675 1260 654 303 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3028 6187 6561 6707 387 Size: 1064 138 1439 3159 374 146 1280 Fragments arranged by size: 3159 1439 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2599 2732 3008 3104 3134 3359 Size: 393 824 143 133 276 96 30 225 Cuts at: 3359 3470 3638 4090 4232 4292 4469 4586 4598 Size: 111 168 452 142 60 177 117 12 Cuts at: 4598 5663 6260 7047 7315 1239 Size: 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 824 787 597 452 393 276 268 225 177 168 143 142 133 117 111 96 60 30 12 EcoNI CCTnn'n_nnAGG Cuts at: 4223 4223 Size: 7600 EcoO109I rG'GnC_Cy Cuts at: 2425 2701 3565 4854 2425 Size: 276 864 1289 5171 Fragments arranged by size: 5171 1289 864 276 EcoRI G'AATT_C Cuts at: 4909 5287 4909 Size: 378 7222 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3470 3701 4616 3470 Size: 231 915 6454 Fragments arranged by size: 6454 915 231 FspI TGC'GCA Cuts at: 4382 6106 4382 Size: 1724 5876 HaeII r_GCGC'y Cuts at: 1678 3820 6747 1678 Size: 2142 2927 2531 Fragments arranged by size: 2927 2531 2142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2289 2321 3014 3046 3078 Size: 32 135 32 717 32 693 32 32 Cuts at: 3078 3130 3162 3854 3886 3926 3958 6241 6273 Size: 52 32 692 32 40 32 2283 32 Cuts at: 6273 6315 6347 1373 Size: 42 32 2626 Fragments arranged by size: 2626 2283 717 693 692 135 52 42 40 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 4944 5190 669 Size: 988 281 753 2253 246 3079 Fragments arranged by size: 3079 2253 988 753 281 246 HindIII A'AGCT_T Cuts at: 748 3389 748 Size: 2641 4959 HpaI GTT'AAC Cuts at: 5190 5190 Size: 7600 KpnI G_GTAC'C Cuts at: 4925 4925 Size: 7600 MfeI C'AATT_G Cuts at: 5199 5199 Size: 7600 MluI A'CGCG_T Cuts at: 7476 7476 Size: 7600 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3345 4323 6714 6898 1305 Size: 2040 978 2391 184 2007 Fragments arranged by size: 2391 2040 2007 978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4319 4535 10 Size: 54 1425 343 2487 216 3075 Fragments arranged by size: 3075 2487 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2358 3164 3437 5558 5917 6076 Size: 648 1146 46 806 273 2121 359 159 Cuts at: 6076 518 Size: 2042 Fragments arranged by size: 2121 2042 1146 806 648 359 273 159 46 MspA1I CmG'CkG Cuts at: 1606 2482 3337 4564 5016 5693 6841 7086 1606 Size: 876 855 1227 452 677 1148 245 2120 Fragments arranged by size: 2120 1227 1148 876 855 677 452 245 NcoI C'CATG_G Cuts at: 513 1065 2313 513 Size: 552 1248 5800 Fragments arranged by size: 5800 1248 552 NdeI CA'TA_TG Cuts at: 387 4293 4886 387 Size: 3906 593 3101 Fragments arranged by size: 3906 3101 593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7600 NotI GC'GGCC_GC Cuts at: 5047 5047 Size: 7600 NsiI A_TGCA'T Cuts at: 3973 3973 Size: 7600 NspI r_CATG'y Cuts at: 1132 1229 3971 4958 6503 1132 Size: 97 2742 987 1545 2229 Fragments arranged by size: 2742 2229 1545 987 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7600 PciI A'CATG_T Cuts at: 6499 6499 Size: 7600 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7600 PfoI T'CCnGG_A Cuts at: 4106 4575 4106 Size: 469 7131 PmeI GTTT'AAAC Cuts at: 4904 4904 Size: 7600 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5669 5701 7182 7214 5669 Size: 32 1481 32 6055 Fragments arranged by size: 6055 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 3565 2425 Size: 1140 6460 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7600 PsiI TTA'TAA Cuts at: 5170 5170 Size: 7600 PstI C_TGCA'G Cuts at: 830 2496 4952 830 Size: 1666 2456 3478 Fragments arranged by size: 3478 2456 1666 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7600 PvuII CAG'CTG Cuts at: 2482 3337 4564 2482 Size: 855 1227 5518 Fragments arranged by size: 5518 1227 855 SalI G'TCGA_C Cuts at: 1936 4942 1936 Size: 3006 4594 SbfI CC_TGCA'GG Cuts at: 4952 4952 Size: 7600 ScaI AGT'ACT Cuts at: 5848 7560 5848 Size: 1712 5888 SexAI A'CCwGG_T Cuts at: 2421 2421 Size: 7600 SfcI C'TryA_G Cuts at: 826 1045 2492 2684 3560 4948 6083 6764 6955 Size: 219 1447 192 876 1388 1135 681 191 Cuts at: 6955 826 Size: 1471 Fragments arranged by size: 1471 1447 1388 1135 876 681 219 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7600 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7600 SmaI CCC'GGG Cuts at: 1872 4927 1872 Size: 3055 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 2608 2642 2948 3212 4220 Size: 197 926 648 17 34 306 264 1008 Cuts at: 4220 4325 4691 5704 6605 6867 820 Size: 105 366 1013 901 262 1553 Fragments arranged by size: 1553 1013 1008 926 901 648 366 306 264 262 197 105 34 17 SnaBI TAC'GTA Cuts at: 493 4897 5391 493 Size: 4404 494 2702 Fragments arranged by size: 4404 2702 494 SpeI A'CTAG_T Cuts at: 152 4711 152 Size: 4559 3041 SphI G_CATG'C Cuts at: 1132 4958 1132 Size: 3826 3774 SspI AAT'ATT Cuts at: 5 52 2332 4093 5524 5 Size: 47 2280 1761 1431 2081 Fragments arranged by size: 2280 2081 1761 1431 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7600 StyI C'CwwG_G Cuts at: 513 1065 2087 2313 5039 513 Size: 552 1022 226 2726 3074 Fragments arranged by size: 3074 2726 1022 552 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2813 4722 5627 5644 5797 5982 6427 Size: 1255 1233 1909 905 17 153 185 445 Cuts at: 6427 325 Size: 1498 Fragments arranged by size: 1909 1498 1255 1233 905 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4714 5846 7558 Size: 274 80 33 51 1443 2737 1132 1712 Cuts at: 7558 96 Size: 138 Fragments arranged by size: 2737 1712 1443 1132 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7560 7592 7560 Size: 32 7568 Tth111I GACn'n_nGTC Cuts at: 1316 2152 1316 Size: 836 6764 XbaI T'CTAG_A Cuts at: 4021 4936 4021 Size: 915 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2756 4465 4823 2756 Size: 1709 358 5533 Fragments arranged by size: 5533 1709 358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7600 XmaI C'CCGG_G Cuts at: 1870 4925 1870 Size: 3055 4545 XmnI GAAnn'nnTTC Cuts at: 5729 5729 Size: 7600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5408 276 Size: 53 83 186 936 3874 2468 Fragments arranged by size: 3874 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI