(Circular) (Six-base) MAPSORT of: pFN21ASDA0375.seq Check: 3962 from: 1 to: 9277 pFN21ASDA0375 9277 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2111 2427 2111 Size: 316 8961 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 5199 7087 278 Size: 53 83 186 936 1966 1697 1888 2468 Fragments arranged by size: 2468 1966 1888 1697 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3462 5862 6620 1937 Size: 1525 2400 758 4594 Fragments arranged by size: 4594 2400 1525 758 Acc65I G'GTAC_C Cuts at: 5835 6598 5835 Size: 763 8514 AclI AA'CG_TT Cuts at: 1563 7404 7777 1563 Size: 5841 373 3063 Fragments arranged by size: 5841 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2857 4979 5153 5509 7340 8724 8992 1632 Size: 1225 2122 174 356 1831 1384 268 1917 Fragments arranged by size: 2122 1917 1831 1384 1225 356 268 174 AfeI AGC'GCT Cuts at: 3506 3506 Size: 9277 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9080 AflIII A'CryG_T Cuts at: 1203 8176 9153 1203 Size: 6973 977 1327 Fragments arranged by size: 6973 1327 977 AgeI A'CCGG_T Cuts at: 8108 8108 Size: 9277 AhdI GACnn_n'nnGTC Cuts at: 8006 8006 Size: 9277 AleI CACnn'nnGTG Cuts at: 1166 3903 1166 Size: 2737 6540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9277 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 2701 Size: 32 9245 AlwNI CAG_nnn'CTG Cuts at: 2589 2972 3152 3320 3428 3431 3974 5509 5614 Size: 383 180 168 108 3 543 1535 105 Cuts at: 5614 6385 7020 8592 2589 Size: 771 635 1572 3274 Fragments arranged by size: 3274 1572 1535 771 635 543 383 180 168 108 105 3 ApaI G_GGCC'C Cuts at: 3440 3605 3778 4159 4395 5656 5672 5812 6117 Size: 165 173 381 236 1261 16 140 305 Cuts at: 6117 3440 Size: 6600 Fragments arranged by size: 6600 1261 381 305 236 173 165 140 16 ApaLI G'TGCA_C Cuts at: 2081 7334 8490 2081 Size: 5253 1156 2868 Fragments arranged by size: 5253 2868 1156 ApoI r'AATT_y Cuts at: 1493 6586 6812 6964 9073 1493 Size: 5093 226 152 2109 1697 Fragments arranged by size: 5093 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7831 160 Size: 872 6799 1606 Fragments arranged by size: 6799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9277 AvaI C'yCGr_G Cuts at: 1870 1943 4152 4287 4635 5072 5649 5655 5811 Size: 73 2209 135 348 437 577 6 156 Cuts at: 5811 5973 6602 1870 Size: 162 629 4545 Fragments arranged by size: 4545 2209 629 577 437 348 162 156 135 73 6 AvrII C'CTAG_G Cuts at: 2197 2197 Size: 9277 BamHI G'GATC_C Cuts at: 3964 5068 6607 6974 3964 Size: 1104 1539 367 6267 Fragments arranged by size: 6267 1539 1104 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 2900 2975 4043 4304 Size: 325 217 627 792 321 75 1068 261 Cuts at: 4304 5720 5835 6508 6598 8053 618 Size: 1416 115 673 90 1455 1842 Fragments arranged by size: 1842 1455 1416 1068 792 673 627 325 321 261 217 115 90 75 BanII G_rGCy'C Cuts at: 1391 2311 3440 3560 3605 3778 3992 4159 4395 Size: 920 1129 120 45 173 214 167 236 Cuts at: 4395 4598 5111 5178 5316 5656 5672 5792 5812 Size: 203 513 67 138 340 16 120 20 Cuts at: 5812 5855 6065 6117 6318 6596 1391 Size: 43 210 52 201 278 4072 Fragments arranged by size: 4072 1129 920 513 340 278 236 214 210 203 201 173 167 138 120 120 67 52 45 43 20 16 BbeI G_GCGC'C Cuts at: 2979 2979 Size: 9277 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5415 928 Size: 971 3516 4790 Fragments arranged by size: 4790 3516 971 BbvCI CC'TCA_GC Cuts at: 3488 4613 5997 6089 3488 Size: 1125 1384 92 6676 Fragments arranged by size: 6676 1384 1125 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7468 7502 1903 Size: 34 5531 34 3678 Fragments arranged by size: 5531 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2557 7169 8379 2557 Size: 4612 1210 3455 Fragments arranged by size: 4612 3455 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9277 BfrBI ATG'CAT Cuts at: 2896 2896 Size: 9277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3261 3662 3829 Size: 107 122 71 631 605 1589 401 167 Cuts at: 3829 4191 4223 5667 5807 7888 136 Size: 362 32 1444 140 2081 1525 Fragments arranged by size: 2081 1589 1525 1444 631 605 401 362 167 140 122 107 71 32 BglII A'GATC_T Cuts at: 9272 9272 Size: 9277 BlpI GC'TnA_GC Cuts at: 3954 6251 6419 6694 3954 Size: 2297 168 275 6537 Fragments arranged by size: 6537 2297 275 168 Bme1580I G_kGCm'C Cuts at: 1790 2085 3143 3440 3605 3778 3794 4159 4395 Size: 295 1058 297 165 173 16 365 236 Cuts at: 4395 5147 5656 5672 5812 6117 6513 7338 8494 Size: 752 509 16 140 305 396 825 1156 Cuts at: 8494 1790 Size: 2573 Fragments arranged by size: 2573 1156 1058 825 752 509 396 365 305 297 295 236 173 165 140 16 16 BmgBI CAC'GTC Cuts at: 1318 5680 1318 Size: 4362 4915 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3578 3945 4665 Size: 416 53 83 399 24 2161 367 720 Cuts at: 4665 7008 7966 442 Size: 2343 958 1753 Fragments arranged by size: 2343 2161 1753 958 720 416 399 367 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2072 5472 6358 2072 Size: 3400 886 4991 Fragments arranged by size: 4991 3400 886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2226 2325 2541 3705 3886 4920 5186 Size: 828 159 99 216 1164 181 1034 266 Cuts at: 5186 5396 5694 6186 6343 6483 7937 1239 Size: 210 298 492 157 140 1454 2579 Fragments arranged by size: 2579 1454 1164 1034 828 492 298 266 216 210 181 159 157 140 99 Bpu10I CC'TnA_GC Cuts at: 2166 2755 3093 3488 4613 5791 5997 6089 2166 Size: 589 338 395 1125 1178 206 92 5354 Fragments arranged by size: 5354 1178 1125 589 395 338 206 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3470 3613 4224 4680 4688 5162 7402 8267 8565 Size: 143 611 456 8 474 2240 865 298 Cuts at: 8565 3470 Size: 4182 Fragments arranged by size: 4182 2240 865 611 474 456 298 143 8 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 4325 7940 882 Size: 617 546 2280 3615 2219 Fragments arranged by size: 3615 2280 2219 617 546 BsaAI yAC'GTr Cuts at: 493 1204 6523 6574 7068 493 Size: 711 5319 51 494 2702 Fragments arranged by size: 5319 2702 711 494 51 BsaBI GATnn'nnATC Cuts at: 9271 9271 Size: 9277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2976 3499 5196 7084 Size: 53 83 186 936 1443 523 1697 1888 Cuts at: 7084 7466 8101 275 Size: 382 635 1451 Fragments arranged by size: 1888 1697 1451 1443 936 635 523 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 5367 7710 8108 8382 8529 8940 4371 Size: 996 2343 398 274 147 411 4708 Fragments arranged by size: 4708 2343 996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2644 2674 3098 3128 5369 Size: 30 165 30 1241 30 424 30 2241 Cuts at: 5369 5399 6523 6553 1178 Size: 30 1124 30 3902 Fragments arranged by size: 3902 2241 1241 1124 424 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2827 3718 5771 6032 6480 1186 Size: 1641 891 2053 261 448 3983 Fragments arranged by size: 3983 2053 1641 891 448 261 BseYI C'CCAG_C Cuts at: 2170 2181 2403 3142 3338 3759 3991 4173 4252 Size: 11 222 739 196 421 232 182 79 Cuts at: 4252 4316 4394 4512 5089 6207 6346 6451 8480 Size: 64 78 118 577 1118 139 105 2029 Cuts at: 8480 8976 2170 Size: 496 2471 Fragments arranged by size: 2471 2029 1118 739 577 496 421 232 222 196 182 139 118 105 79 78 64 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 2238 5472 6398 1163 Size: 573 480 22 3234 926 4042 Fragments arranged by size: 4042 3234 926 573 480 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6727 7488 8516 1540 Size: 275 186 4726 761 1028 2301 Fragments arranged by size: 4726 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2141 4427 5316 5855 7338 7423 8494 2085 Size: 56 2286 889 539 1483 85 1071 2868 Fragments arranged by size: 2868 2286 1483 1071 889 539 85 56 BsmI GAATG_Cn' Cuts at: 6788 6881 6788 Size: 93 9184 BsmBI CGTCTCn'nnnn_ Cuts at: 5670 5670 Size: 9277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2141 2311 3143 3440 3560 3605 Size: 399 295 56 170 832 297 120 45 Cuts at: 3605 3778 3794 3992 4159 4395 4427 4598 5111 Size: 173 16 198 167 236 32 171 513 Cuts at: 5111 5147 5178 5316 5656 5672 5792 5812 5855 Size: 36 31 138 340 16 120 20 43 Cuts at: 5855 6065 6117 6318 6513 6596 7338 7423 8494 Size: 210 52 201 195 83 742 85 1071 Cuts at: 8494 1391 Size: 2174 Fragments arranged by size: 2174 1071 832 742 513 399 340 297 295 236 210 201 198 195 173 171 170 167 138 120 120 85 83 56 52 45 43 36 32 31 20 16 16 BspEI T'CCGG_A Cuts at: 4371 8940 4371 Size: 4569 4708 BspHI T'CATG_A Cuts at: 7166 8896 7166 Size: 1730 7547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2111 2427 4901 4998 5622 6632 844 Size: 1267 316 2474 97 624 1010 3489 Fragments arranged by size: 3489 2474 1267 1010 624 316 97 BsrBI CCG'CTC Cuts at: 1394 1823 5704 7164 1394 Size: 429 3881 1460 3507 Fragments arranged by size: 3881 3507 1460 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7772 7946 65 Size: 1183 6524 174 1396 Fragments arranged by size: 6524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2595 4560 4854 5550 5825 6404 7921 Size: 110 785 1965 294 696 275 579 1517 Cuts at: 7921 8108 1700 Size: 187 2869 Fragments arranged by size: 2869 1965 1517 785 696 579 294 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9277 BssSI C'ACGA_G Cuts at: 5898 7337 8349 5898 Size: 1439 1012 6826 Fragments arranged by size: 6826 1439 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3428 5554 794 Size: 2634 2126 4517 Fragments arranged by size: 4517 2634 2126 BstBI TT'CG_AA Cuts at: 2007 8081 2007 Size: 6074 3203 BstEII G'GTnAC_C Cuts at: 1184 2366 2866 3643 1184 Size: 1182 500 777 6818 Fragments arranged by size: 6818 1182 777 500 BstXI CCAn_nnnn'nTGG Cuts at: 2423 3826 4141 2423 Size: 1403 315 7559 Fragments arranged by size: 7559 1403 315 BstYI r'GATC_y Cuts at: 1972 2989 3964 5068 6607 6974 7359 7376 8828 Size: 1017 975 1104 1539 367 385 17 1452 Cuts at: 8828 8914 8926 9272 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1539 1452 1104 1017 975 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3463 3463 Size: 9277 Bsu36I CC'TnA_GG Cuts at: 4261 4261 Size: 9277 BtgI C'CryG_G Cuts at: 513 1065 2022 2495 2817 3612 4469 4567 5225 Size: 552 957 473 322 795 857 98 658 Cuts at: 5225 6487 513 Size: 1262 3303 Fragments arranged by size: 3303 1262 957 857 795 658 552 473 322 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4329 5011 487 Size: 683 56 3103 682 4753 Fragments arranged by size: 4753 3103 683 682 56 BtsI GCAGTG_nn' Cuts at: 2083 2111 2579 2587 2665 2767 3153 3408 4094 Size: 28 468 8 78 102 386 255 686 Cuts at: 4094 4878 6793 7587 7607 2083 Size: 784 1915 794 20 3753 Fragments arranged by size: 3753 1915 794 784 686 468 386 255 102 78 28 20 8 ClaI AT'CG_AT Cuts at: 1554 5868 1554 Size: 4314 4963 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3430 3465 4731 4766 529 Size: 35 2866 35 1266 35 5040 Fragments arranged by size: 5040 2866 1266 35 35 35 DraI TTT'AAA Cuts at: 6581 6581 Size: 9277 DraIII CAC_nnn'GTG Cuts at: 3131 3710 4422 3131 Size: 579 712 7986 Fragments arranged by size: 7986 712 579 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8284 809 Size: 507 6968 1802 Fragments arranged by size: 6968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2431 2597 3195 Size: 54 1425 50 275 18 601 166 598 Cuts at: 3195 3615 3663 4143 4340 4659 4864 6081 6399 Size: 420 48 480 197 319 205 1217 318 Cuts at: 6399 6724 7613 9242 8 Size: 325 889 1629 43 Fragments arranged by size: 1629 1425 1217 889 601 598 480 420 325 319 318 275 205 197 166 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6724 1537 Size: 275 4912 4090 Fragments arranged by size: 4912 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2290 2953 3091 3271 4411 5478 5733 5739 Size: 937 663 138 180 1140 1067 255 6 Cuts at: 5739 5748 5760 7207 1353 Size: 9 12 1447 3423 Fragments arranged by size: 3423 1447 1140 1067 937 663 255 180 138 12 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3539 7864 8238 8384 387 Size: 1064 138 1950 4325 374 146 1280 Fragments arranged by size: 4325 1950 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5314 5853 5314 Size: 539 8738 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2226 2325 2541 2857 3705 3886 Size: 393 435 159 99 216 316 848 181 Cuts at: 3886 4920 4979 5153 5186 5396 5509 5694 6186 Size: 1034 59 174 33 210 113 185 492 Cuts at: 6186 6343 6483 7340 7937 8724 8992 1239 Size: 157 140 857 597 787 268 1524 Fragments arranged by size: 1524 1034 857 848 787 597 492 435 393 316 268 216 210 185 181 174 159 157 140 113 99 59 33 EcoO109I rG'GnC_Cy Cuts at: 2300 2642 3317 3335 3437 3601 3602 3774 3775 Size: 342 675 18 102 164 1 172 1 Cuts at: 3775 3893 3944 3971 4156 4909 5272 5611 5668 Size: 118 51 27 185 753 363 339 57 Cuts at: 5668 5669 5951 6022 6113 6147 2300 Size: 1 282 71 91 34 5430 Fragments arranged by size: 5430 753 675 363 342 339 282 185 172 164 118 102 91 71 57 51 34 27 18 1 1 1 EcoRI G'AATT_C Cuts at: 6586 6964 6586 Size: 378 8899 FspI TGC'GCA Cuts at: 7783 7783 Size: 9277 HaeII r_GCGC'y Cuts at: 1678 2979 3508 8424 1678 Size: 1301 529 4916 2531 Fragments arranged by size: 4916 2531 1301 529 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 4596 4628 4757 Size: 32 135 32 697 32 2295 32 129 Cuts at: 4757 4789 4952 4984 6082 6114 6523 6555 7918 Size: 32 163 32 1098 32 409 32 1363 Cuts at: 7918 7950 7992 8024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2295 1363 1098 697 409 163 135 129 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5023 6621 6867 669 Size: 988 281 3085 1598 246 3079 Fragments arranged by size: 3085 3079 1598 988 281 246 HindIII A'AGCT_T Cuts at: 748 4579 748 Size: 3831 5446 HpaI GTT'AAC Cuts at: 5023 6867 5023 Size: 1844 7433 KasI G'GCGC_C Cuts at: 2975 2975 Size: 9277 KpnI G_GTAC'C Cuts at: 5839 6602 5839 Size: 763 8514 MfeI C'AATT_G Cuts at: 6876 6876 Size: 9277 MluI A'CGCG_T Cuts at: 9153 9153 Size: 9277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6298 6572 8391 8575 1305 Size: 4993 274 1819 184 2007 Fragments arranged by size: 4993 2007 1819 274 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2433 3197 3665 4145 4661 Size: 54 1425 343 601 764 468 480 516 Cuts at: 4661 4866 6401 10 Size: 205 1535 2886 Fragments arranged by size: 2886 1535 1425 764 601 516 480 468 343 205 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 3903 5030 7235 7594 7753 518 Size: 648 1255 1482 1127 2205 359 159 2042 Fragments arranged by size: 2205 2042 1482 1255 1127 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2464 2775 2807 3428 3958 4141 4940 5578 Size: 858 311 32 621 530 183 799 638 Cuts at: 5578 5644 6119 6211 6418 6693 7370 8518 8763 Size: 66 475 92 207 275 677 1148 245 Cuts at: 8763 1606 Size: 2120 Fragments arranged by size: 2120 1148 858 799 677 638 621 530 475 311 275 245 207 183 92 66 32 NaeI GCC'GGC Cuts at: 2597 4856 5827 2597 Size: 2259 971 6047 Fragments arranged by size: 6047 2259 971 NarI GG'CG_CC Cuts at: 2976 2976 Size: 9277 NcoI C'CATG_G Cuts at: 513 1065 2022 2495 2817 3612 4469 4567 5225 Size: 552 957 473 322 795 857 98 658 Cuts at: 5225 513 Size: 4565 Fragments arranged by size: 4565 957 857 795 658 552 473 322 98 NdeI CA'TA_TG Cuts at: 387 387 Size: 9277 NgoMIV G'CCGG_C Cuts at: 2595 4854 5825 2595 Size: 2259 971 6047 Fragments arranged by size: 6047 2259 971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9277 NotI GC'GGCC_GC Cuts at: 6724 6724 Size: 9277 NsiI A_TGCA'T Cuts at: 2898 2898 Size: 9277 NspI r_CATG'y Cuts at: 1132 1229 5224 6635 8180 1132 Size: 97 3995 1411 1545 2229 Fragments arranged by size: 3995 2229 1545 1411 97 PasI CC'CwG_GG Cuts at: 1348 3983 4351 5436 6025 1348 Size: 2635 368 1085 589 4600 Fragments arranged by size: 4600 2635 1085 589 368 PciI A'CATG_T Cuts at: 8176 8176 Size: 9277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2363 2474 2818 3320 6280 6397 6554 1108 Size: 1255 111 344 502 2960 117 157 3831 Fragments arranged by size: 3831 2960 1255 502 344 157 117 111 PfoI T'CCnGG_A Cuts at: 2242 4905 2242 Size: 2663 6614 PmeI GTTT'AAAC Cuts at: 6581 6581 Size: 9277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7346 7378 8859 8891 7346 Size: 32 1481 32 7732 Fragments arranged by size: 7732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2642 3893 4909 5272 5611 5951 2642 Size: 1251 1016 363 339 340 5968 Fragments arranged by size: 5968 1251 1016 363 340 339 PshAI GACnn'nnGTC Cuts at: 1613 3861 1613 Size: 2248 7029 PsiI TTA'TAA Cuts at: 6847 6847 Size: 9277 PspOMI G'GGCC_C Cuts at: 3436 3601 3774 4155 4391 5652 5668 5808 6113 Size: 165 173 381 236 1261 16 140 305 Cuts at: 6113 3436 Size: 6600 Fragments arranged by size: 6600 1261 381 305 236 173 165 140 16 PstI C_TGCA'G Cuts at: 830 2340 2780 2942 2960 2997 3534 3610 4199 Size: 1510 440 162 18 37 537 76 589 Cuts at: 4199 5643 6333 6485 6629 830 Size: 1444 690 152 144 3478 Fragments arranged by size: 3478 1510 1444 690 589 537 440 162 152 144 76 37 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9277 PvuII CAG'CTG Cuts at: 2464 2807 3428 3958 4141 4940 5644 6418 2464 Size: 343 621 530 183 799 704 774 5323 Fragments arranged by size: 5323 799 774 704 621 530 343 183 SacI G_AGCT'C Cuts at: 5316 5855 5316 Size: 539 8738 SalI G'TCGA_C Cuts at: 1936 6619 1936 Size: 4683 4594 SanDI GG'GwC_CC Cuts at: 3893 3893 Size: 9277 SapI GCTCTTCn'nnn_ Cuts at: 3271 5478 3271 Size: 2207 7070 SbfI CC_TGCA'GG Cuts at: 6333 6629 6333 Size: 296 8981 ScaI AGT'ACT Cuts at: 5138 7525 9237 5138 Size: 2387 1712 5178 Fragments arranged by size: 5178 2387 1712 SexAI A'CCwGG_T Cuts at: 2644 4744 5124 2644 Size: 2100 380 6797 Fragments arranged by size: 6797 2100 380 SfcI C'TryA_G Cuts at: 826 1045 2262 2336 2776 2938 2956 2993 3530 Size: 219 1217 74 440 162 18 37 537 Cuts at: 3530 3606 3799 4069 4180 4195 5501 5639 6329 Size: 76 193 270 111 15 1306 138 690 Cuts at: 6329 6481 6625 7760 8441 8632 826 Size: 152 144 1135 681 191 1471 Fragments arranged by size: 1471 1306 1217 1135 690 681 537 440 270 219 193 191 162 152 144 138 111 76 74 37 18 15 SfiI GGCCn_nnn'nGGCC Cuts at: 5667 5667 Size: 9277 SfoI GGC'GCC Cuts at: 2977 2977 Size: 9277 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9277 SmaI CCC'GGG Cuts at: 1872 4154 4637 5074 5651 5657 6604 1872 Size: 2282 483 437 577 6 947 4545 Fragments arranged by size: 4545 2282 947 577 483 437 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3449 3592 4239 4695 4703 5177 Size: 197 926 1506 143 647 456 8 474 Cuts at: 5177 7381 8282 8544 820 Size: 2204 901 262 1553 Fragments arranged by size: 2204 1553 1506 926 901 647 474 456 262 197 143 8 SnaBI TAC'GTA Cuts at: 493 6574 7068 493 Size: 6081 494 2702 Fragments arranged by size: 6081 2702 494 SpeI A'CTAG_T Cuts at: 152 6277 152 Size: 6125 3152 SphI G_CATG'C Cuts at: 1132 6635 1132 Size: 5503 3774 SrfI GCCC'GGGC Cuts at: 4154 5651 4154 Size: 1497 7780 SspI AAT'ATT Cuts at: 5 52 7201 5 Size: 47 7149 2081 Fragments arranged by size: 7149 2081 47 StuI AGG'CCT Cuts at: 1517 5183 6002 1517 Size: 3666 819 4792 Fragments arranged by size: 4792 3666 819 StyI C'CwwG_G Cuts at: 513 1065 2022 2197 2495 2559 2732 2817 3612 Size: 552 957 175 298 64 173 85 795 Cuts at: 3612 3666 4146 4469 4567 5225 5268 5695 6318 Size: 54 480 323 98 658 43 427 623 Cuts at: 6318 6716 513 Size: 398 3074 Fragments arranged by size: 3074 957 795 658 623 552 480 427 398 323 298 175 173 98 85 64 54 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 2339 2417 3431 3649 3760 3832 Size: 1255 615 144 78 1014 218 111 72 Cuts at: 3832 3893 4782 5708 7304 7321 7474 7659 8104 Size: 61 889 926 1596 17 153 185 445 Cuts at: 8104 325 Size: 1498 Fragments arranged by size: 1596 1498 1255 1014 926 889 615 445 218 185 153 144 111 78 72 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5136 5188 7523 Size: 274 80 33 51 1443 3159 52 2335 Cuts at: 7523 9235 96 Size: 1712 138 Fragments arranged by size: 3159 2335 1712 1443 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2644 2676 4086 4118 4154 4186 9237 9269 2644 Size: 32 1410 32 36 32 5051 32 2652 Fragments arranged by size: 5051 2652 1410 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9277 XbaI T'CTAG_A Cuts at: 6613 6613 Size: 9277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2969 3867 5641 5669 2969 Size: 898 1774 28 6577 Fragments arranged by size: 6577 1774 898 28 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9277 XmaI C'CCGG_G Cuts at: 1870 4152 4635 5072 5649 5655 6602 1870 Size: 2282 483 437 577 6 947 4545 Fragments arranged by size: 4545 2282 947 577 483 437 6 XmnI GAAnn'nnTTC Cuts at: 5098 7406 5098 Size: 2308 6969 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 5197 7085 276 Size: 53 83 186 936 1966 1697 1888 2468 Fragments arranged by size: 2468 1966 1888 1697 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsiWI EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SwaI