(Circular) (Six-base) MAPSORT of: pFN21ASDA0331.seq Check: 2707 from: 1 to: 7054 pFN21ASDA0331 7054 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3372 4864 278 Size: 53 83 186 936 1836 1492 2468 Fragments arranged by size: 2468 1836 1492 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4397 1937 Size: 2460 4594 Acc65I G'GTAC_C Cuts at: 4375 4375 Size: 7054 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3452 3798 5117 6501 6769 1632 Size: 1820 346 1319 1384 268 1917 Fragments arranged by size: 1917 1820 1384 1319 346 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 3057 3582 5953 6930 1203 Size: 1854 525 2371 977 1327 Fragments arranged by size: 2371 1854 1327 977 525 AgeI A'CCGG_T Cuts at: 5885 5885 Size: 7054 AhdI GACnn_n'nnGTC Cuts at: 2088 5783 2088 Size: 3695 3359 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7054 AlwNI CAG_nnn'CTG Cuts at: 3968 4797 6369 3968 Size: 829 1572 4653 Fragments arranged by size: 4653 1572 829 ApaLI G'TGCA_C Cuts at: 4222 5111 6267 4222 Size: 889 1156 5009 Fragments arranged by size: 5009 1156 889 ApoI r'AATT_y Cuts at: 1493 2699 4157 4363 4589 4741 6850 1493 Size: 1206 1458 206 226 152 2109 1697 Fragments arranged by size: 2109 1697 1458 1206 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 5608 160 Size: 872 4576 1606 Fragments arranged by size: 4576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 3618 4379 1870 Size: 73 1675 761 4545 Fragments arranged by size: 4545 1675 761 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3239 3272 3239 Size: 33 7021 BamHI G'GATC_C Cuts at: 2479 3481 4384 4751 2479 Size: 1002 903 367 4782 Fragments arranged by size: 4782 1002 903 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4375 5830 618 Size: 325 217 627 2588 1455 1842 Fragments arranged by size: 2588 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 4373 1391 Size: 751 2231 4072 Fragments arranged by size: 4072 2231 751 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2739 3908 928 Size: 971 840 1169 4074 Fragments arranged by size: 4074 1169 971 840 BbvCI CC'TCA_GC Cuts at: 2264 3351 2264 Size: 1087 5967 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5245 5279 1903 Size: 34 3308 34 3678 Fragments arranged by size: 3678 3308 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 1548 Size: 7054 BfrBI ATG'CAT Cuts at: 2337 2337 Size: 7054 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5665 136 Size: 107 122 71 631 605 3993 1525 Fragments arranged by size: 3993 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2507 7049 2507 Size: 4542 2512 BlpI GC'TnA_GC Cuts at: 3729 4471 3729 Size: 742 6312 Bme1580I G_kGCm'C Cuts at: 1790 2040 4226 5115 6271 1790 Size: 250 2186 889 1156 2573 Fragments arranged by size: 2573 2186 1156 889 250 BmgBI CAC'GTC Cuts at: 1318 3878 1318 Size: 2560 4494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4785 5743 442 Size: 416 53 83 399 24 3368 958 1753 Fragments arranged by size: 3368 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2334 2334 Size: 7054 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2448 2629 2801 5714 1239 Size: 854 355 181 172 2913 2579 Fragments arranged by size: 2913 2579 854 355 181 172 Bpu10I CC'TnA_GC Cuts at: 2264 3351 4311 2264 Size: 1087 960 5007 Fragments arranged by size: 5007 1087 960 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3543 5179 6044 6342 3543 Size: 1636 865 298 4255 Fragments arranged by size: 4255 1636 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3615 5717 882 Size: 617 2116 2102 2219 Fragments arranged by size: 2219 2116 2102 617 BsaAI yAC'GTr Cuts at: 493 1204 4351 4845 493 Size: 711 3147 494 2702 Fragments arranged by size: 3147 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2217 7048 2217 Size: 4831 2223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3369 4861 5243 5878 Size: 53 83 186 936 1836 1492 382 635 Cuts at: 5878 275 Size: 1451 Fragments arranged by size: 1836 1492 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3696 5487 5885 6159 6306 6717 3696 Size: 1791 398 274 147 411 4033 Fragments arranged by size: 4033 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 1178 Size: 30 165 30 1128 30 5671 Fragments arranged by size: 5671 1128 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2539 3908 4061 4097 1186 Size: 1353 1369 153 36 4143 Fragments arranged by size: 4143 1369 1353 153 36 BseYI C'CCAG_C Cuts at: 2543 3968 6257 6753 2543 Size: 1425 2289 496 2844 Fragments arranged by size: 2844 2289 1425 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6481 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4504 5265 6293 1540 Size: 275 186 2503 761 1028 2301 Fragments arranged by size: 2503 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2879 4226 5115 5200 6271 2142 Size: 737 1347 889 85 1071 2925 Fragments arranged by size: 2925 1347 1071 889 737 85 BsmI GAATG_Cn' Cuts at: 2339 3066 4565 4658 2339 Size: 727 1499 93 4735 Fragments arranged by size: 4735 1499 727 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2610 2610 Size: 7054 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2142 2879 4226 4373 5115 5200 Size: 399 250 102 737 1347 147 742 85 Cuts at: 5200 6271 1391 Size: 1071 2174 Fragments arranged by size: 2174 1347 1071 742 737 399 250 147 102 85 BspEI T'CCGG_A Cuts at: 3696 6717 3696 Size: 3021 4033 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3280 4409 844 Size: 2436 1129 3489 Fragments arranged by size: 3489 2436 1129 BsrBI CCG'CTC Cuts at: 1394 1823 4941 1394 Size: 429 3118 3507 Fragments arranged by size: 3507 3118 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5549 5723 65 Size: 1183 4301 174 1396 Fragments arranged by size: 4301 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5698 5885 1700 Size: 110 3888 187 2869 Fragments arranged by size: 3888 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3865 96 Size: 3769 3285 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 5114 6126 5114 Size: 1012 6042 BstAPI GCAn_nnn'nTGC Cuts at: 794 3651 794 Size: 2857 4197 BstBI TT'CG_AA Cuts at: 2007 5858 2007 Size: 3851 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7054 BstYI r'GATC_y Cuts at: 1972 2479 2507 3481 3539 4384 4751 5136 5153 Size: 507 28 974 58 845 367 385 17 Cuts at: 5153 6605 6691 6703 7049 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 974 845 507 385 367 346 86 58 28 17 12 Bsu36I CC'TnA_GG Cuts at: 3947 4284 3947 Size: 337 6717 BtgI C'CryG_G Cuts at: 513 1065 2022 2031 4145 513 Size: 552 957 9 2114 3422 Fragments arranged by size: 3422 2114 957 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6315 Fragments arranged by size: 6315 683 56 BtsI GCAGTG_nn' Cuts at: 3740 4570 5364 5384 3740 Size: 830 794 20 5410 Fragments arranged by size: 5410 830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2551 2586 529 Size: 35 1987 35 4997 Fragments arranged by size: 4997 1987 35 35 DraI TTT'AAA Cuts at: 4358 4358 Size: 7054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6061 809 Size: 507 4745 1802 Fragments arranged by size: 4745 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2306 3358 4501 Size: 54 1425 50 275 18 476 1052 1143 Cuts at: 4501 5390 7019 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1143 1052 889 476 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4501 1537 Size: 275 2689 4090 Fragments arranged by size: 4090 2689 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 3947 4038 4984 1353 Size: 884 1710 91 946 3423 Fragments arranged by size: 3423 1710 946 884 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5641 6015 6161 387 Size: 1064 138 4052 374 146 1280 Fragments arranged by size: 4052 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 7054 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2448 2629 2801 3452 3798 5117 Size: 393 461 355 181 172 651 346 1319 Cuts at: 5117 5714 6501 6769 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1319 787 651 597 461 393 355 346 268 181 172 EcoO109I rG'GnC_Cy Cuts at: 2248 3107 3972 2248 Size: 859 865 5330 Fragments arranged by size: 5330 865 859 EcoRI G'AATT_C Cuts at: 4157 4363 4741 4157 Size: 206 378 6470 Fragments arranged by size: 6470 378 206 EcoRV GAT'ATC Cuts at: 2468 2468 Size: 7054 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4177 4177 Size: 7054 FspI TGC'GCA Cuts at: 5560 5560 Size: 7054 HaeII r_GCGC'y Cuts at: 1678 6201 1678 Size: 4523 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 2531 2563 3632 Size: 32 135 32 502 32 425 32 1069 Cuts at: 3632 3664 4093 4125 5695 5727 5769 5801 1373 Size: 32 429 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1570 1069 502 429 425 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4398 4644 669 Size: 988 281 2460 246 3079 Fragments arranged by size: 3079 2460 988 281 246 HindIII A'AGCT_T Cuts at: 748 4244 748 Size: 3496 3558 HpaI GTT'AAC Cuts at: 4644 4644 Size: 7054 KpnI G_GTAC'C Cuts at: 4379 4379 Size: 7054 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 6168 6352 1305 Size: 1138 3725 184 2007 Fragments arranged by size: 3725 2007 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3360 10 Size: 54 1425 343 1528 3704 Fragments arranged by size: 3704 1528 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2673 3576 3720 5012 5371 5530 518 Size: 648 1507 903 144 1292 359 159 2042 Fragments arranged by size: 2042 1507 1292 903 648 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2033 2099 3529 4168 4470 5147 6295 6540 Size: 427 66 1430 639 302 677 1148 245 Cuts at: 6540 1606 Size: 2120 Fragments arranged by size: 2120 1430 1148 677 639 427 302 245 66 NcoI C'CATG_G Cuts at: 513 1065 2022 4145 513 Size: 552 957 2123 3422 Fragments arranged by size: 3422 2123 957 552 NdeI CA'TA_TG Cuts at: 387 3091 387 Size: 2704 4350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7054 NotI GC'GGCC_GC Cuts at: 4501 4501 Size: 7054 NsiI A_TGCA'T Cuts at: 2339 2339 Size: 7054 NspI r_CATG'y Cuts at: 1132 1229 3041 3061 3586 3683 4412 5957 1132 Size: 97 1812 20 525 97 729 1545 2229 Fragments arranged by size: 2229 1812 1545 729 525 97 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7054 PciI A'CATG_T Cuts at: 3057 3582 5953 3057 Size: 525 2371 4158 Fragments arranged by size: 4158 2371 525 PflMI CCAn_nnn'nTGG Cuts at: 1108 3158 3433 4195 1108 Size: 2050 275 762 3967 Fragments arranged by size: 3967 2050 762 275 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5123 5155 6636 6668 5123 Size: 32 1481 32 5509 Fragments arranged by size: 5509 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 3107 3972 2248 Size: 859 865 5330 Fragments arranged by size: 5330 865 859 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7054 PsiI TTA'TAA Cuts at: 4624 4624 Size: 7054 PstI C_TGCA'G Cuts at: 830 4406 830 Size: 3576 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 PvuII CAG'CTG Cuts at: 2099 3529 4168 2099 Size: 1430 639 4985 Fragments arranged by size: 4985 1430 639 RsrII CG'GwC_CG Cuts at: 4015 4015 Size: 7054 SacI G_AGCT'C Cuts at: 2142 2142 Size: 7054 SacII CC_GC'GG Cuts at: 2034 2034 Size: 7054 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2237 Size: 7054 SbfI CC_TGCA'GG Cuts at: 4406 4406 Size: 7054 ScaI AGT'ACT Cuts at: 3814 5302 7014 3814 Size: 1488 1712 3854 Fragments arranged by size: 3854 1712 1488 SfcI C'TryA_G Cuts at: 826 1045 2596 4402 5537 6218 6409 826 Size: 219 1551 1806 1135 681 191 1471 Fragments arranged by size: 1806 1551 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7054 SmaI CCC'GGG Cuts at: 1872 4381 1872 Size: 2509 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3558 3618 5158 6059 6321 820 Size: 197 926 1615 60 1540 901 262 1553 Fragments arranged by size: 1615 1553 1540 926 901 262 197 60 SnaBI TAC'GTA Cuts at: 493 4351 4845 493 Size: 3858 494 2702 Fragments arranged by size: 3858 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 3041 4412 1132 Size: 1909 1371 3774 Fragments arranged by size: 3774 1909 1371 SspI AAT'ATT Cuts at: 5 52 2166 3025 3291 4978 5 Size: 47 2114 859 266 1687 2081 Fragments arranged by size: 2114 2081 1687 859 266 47 StuI AGG'CCT Cuts at: 1517 3157 1517 Size: 1640 5414 StyI C'CwwG_G Cuts at: 513 1065 2022 3152 3204 3931 4029 4145 4493 Size: 552 957 1130 52 727 98 116 348 Cuts at: 4493 513 Size: 3074 Fragments arranged by size: 3074 1130 957 727 552 348 116 98 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2518 5081 5098 5251 5436 5881 325 Size: 1255 938 2563 17 153 185 445 1498 Fragments arranged by size: 2563 1498 1255 938 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2312 3812 3865 Size: 274 80 33 51 1443 335 1500 53 Cuts at: 3865 5300 7012 96 Size: 1435 1712 138 Fragments arranged by size: 1712 1500 1443 1435 335 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7014 7046 7014 Size: 32 7022 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7054 XbaI T'CTAG_A Cuts at: 3457 4390 3457 Size: 933 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4336 4336 Size: 7054 XhoI C'TCGA_G Cuts at: 1943 3618 1943 Size: 1675 5379 XmaI C'CCGG_G Cuts at: 1870 4379 1870 Size: 2509 4545 XmnI GAAnn'nnTTC Cuts at: 3453 3465 5183 3453 Size: 12 1718 5324 Fragments arranged by size: 5324 1718 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3370 4862 276 Size: 53 83 186 936 1836 1492 2468 Fragments arranged by size: 2468 1836 1492 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI SanDI SexAI SfiI SfoI SrfI SwaI