Restriction map for
pFN21ASDA0317
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AvrII BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HgaI
HpaI KpnI KspI MamI MluI
MroI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccII 112 CGCG CG^CG
AcyI 1121 GGCGCC G[GA]^CG[CT]C
Alw44I 2413 GTGCAC G^TGCAC
AlwNI 26 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2413 GTGCAC G^TGCAC
AspI 1177 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 395 TGCGCA TGC^GCA
BamHI 165 GGATCC G^GATCC
BbeI 1121 GGCGCC GGCGC^C
BbrPI 80 CACGTG CAC^GTG
BbsI 1003 GAAGAC GAAGAC(2/6)
BlpI 1236 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1236 GCTCAGC GC^T[ACGT]AGC
BpuAI 1003 GAAGAC GAAGAC(2/6)
BsaHI 1121 GGCGCC G[GA]^CG[CT]C
BsaI 1309 GAGACC GGTCTC(1/5)
BsaWI 128 ACCGGA [AT]^CCGG[AT]
BsmBI 1421 CGTCTC CGTCTC(1/5)
BspLU11I 2365 ACATGT A^CATGT
BsrDI 857 GCAATG GCAATG(2/0)
BsrFI 971 GCCGGC [GA]^CCGG[CT]
BssSI 1672 CTCGTG CACGAG(-5/-1)
BstAPI 2415 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 112 CGCG CG^CG
BstYI 165 GGATCC [GA]^GATC[CT]
CelII 1236 GCTCAGC GC^T[ACGT]AGC
Cfr10I 971 GCCGGC [GA]^CCGG[CT]
DraI 1977 TTTAAA TTT^AAA
DrdI 814 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 931 CCATGG C^C[GA][CT]GG
EcoNI 825 CCTAAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1810 GAATTC G^AATTC
EcoT22I 1777 ATGCAT ATGCA^T
FspI 395 TGCGCA TGC^GCA
Hin1I 1121 GGCGCC G[GA]^CG[CT]C
HincII 988 GTTGAC GT[CT]^[GA]AC
HindII 988 GTTGAC GT[CT]^[GA]AC
HindIII 427 AAGCTT A^AGCTT
KasI 1121 GGCGCC G^GCGCC
MfeI 1903 CAATTG C^AATTG
MflI 165 GGATCC [GA]^GATC[CT]
MunI 1903 CAATTG C^AATTG
MvnI 112 CGCG CG^CG
NaeI 971 GCCGGC GCC^GGC
NarI 1121 GGCGCC GG^CGCC
NcoI 931 CCATGG C^CATGG
NgoMIV 971 GCCGGC G^CCGGC
NheI 318 GCTAGC G^CTAGC
NsiI 1777 ATGCAT ATGCA^T
PaeR7I 752 CTCGAG C^TCGAG
PflFI 1177 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 219 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 80 CACGTG CAC^GTG
PmlI 80 CACGTG CAC^GTG
PpuMI 200 AGGACCC [GA]G^G[AT]CC[CT]
PstI 2425 CTGCAG CTGCA^G
SapI 1572 GCTCTTC GCTCTTC(1/4)
SfoI 1121 GGCGCC GGC^GCC
SphI 2462 GCATGC GCATG^C
StuI 2043 AGGCCT AGG^CCT
SwaI 1976 ATTTAAAT ATTT^AAAT
Tth111I 1177 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 219 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 752 CTCGAG C^TCGAG
XhoII 165 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 81 ACGTGT A^C[GA][CT]GT
2365 ACATGT
AlwI 166 GATCC GGATC(4/5)
165 GGATC
AvaI 572 CCCGAG C^[CT]CG[GA]G
752 CTCGAG
BalI 2003 TGGCCA TGG^CCA
2432 TGGCCA
BanI 935 GGCACC G^G[CT][GA]CC
1121 GGCGCC
BsaAI 80 CACGTG [CT]AC^GT[GA]
145 TACGTG
BsgI 272 CTGCAC GTGCAG(16/14)
2357 CTGCAC
BsoBI 572 CCCGAG C^[CT]CG[GA]G
752 CTCGAG
BspMI 938 ACCTGC ACCTGC(4/8)
1353 GCAGGT
DpnII 166 GATC ^GATC
1110 GATC
DraII 200 AGGACCC [GA]G^G[ACGT]CC[CT]
1512 AGGGCCT
EcoO109I 200 AGGACCC [GA]G^G[ACGT]CC[CT]
1512 AGGGCCT
EcoT14I 931 CCATGG C^C[AT][AT]GG
1167 CCTTGG
MboI 166 GATC ^GATC
1110 GATC
MluNI 2003 TGGCCA TGG^CCA
2432 TGGCCA
MscI 2003 TGGCCA TGG^CCA
2432 TGGCCA
NdeI 1360 CATATG CA^TATG
2393 CATATG
NdeII 166 GATC ^GATC
1110 GATC
Sau3AI 166 GATC ^GATC
1110 GATC
ScaI 673 AGTACT AGT^ACT
1787 AGTACT
SfaNI 2421 GATGC GCATC(5/9)
1611 GCATC
SfcI 918 CTGTAG C^T[GA][CT]AG
2425 CTGCAG
StyI 931 CCATGG C^C[AT][AT]GG
1167 CCTTGG
TfiI 20 GAATC G^A[AT]TC
1417 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV NspI PleI
PvuII RcaI RsaI SacI Sau96I
ScrFI SmlI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI