(Circular) (Six-base) MAPSORT of: pFN21ASDA0317.seq Check: 9062 from: 1 to: 7198 pFN21ASDA0317 7198 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2970 2970 Size: 7198 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5008 278 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4541 1937 Size: 2604 4594 Acc65I G'GTAC_C Cuts at: 4519 4519 Size: 7198 AclI AA'CG_TT Cuts at: 1563 5325 5698 1563 Size: 3762 373 3063 Fragments arranged by size: 3762 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 2877 3149 3434 4262 5261 6645 6913 Size: 728 517 272 285 828 999 1384 268 Cuts at: 6913 1632 Size: 1917 Fragments arranged by size: 1917 1384 999 828 728 517 285 272 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7001 AflIII A'CryG_T Cuts at: 1203 2104 4388 6097 7074 1203 Size: 901 2284 1709 977 1327 Fragments arranged by size: 2284 1709 1327 977 901 AgeI A'CCGG_T Cuts at: 6029 6029 Size: 7198 AhdI GACnn_n'nnGTC Cuts at: 5927 5927 Size: 7198 AleI CACnn'nnGTG Cuts at: 1166 3224 1166 Size: 2058 5140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7198 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2304 2336 2496 2528 2304 Size: 32 160 32 6974 Fragments arranged by size: 6974 160 32 32 AlwNI CAG_nnn'CTG Cuts at: 2054 4941 6513 2054 Size: 2887 1572 2739 Fragments arranged by size: 2887 2739 1572 ApaLI G'TGCA_C Cuts at: 4436 5255 6411 4436 Size: 819 1156 5223 Fragments arranged by size: 5223 1156 819 ApoI r'AATT_y Cuts at: 1493 2352 2490 2831 3179 3463 3833 4003 4507 Size: 859 138 341 348 284 370 170 504 Cuts at: 4507 4733 4885 6994 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 859 504 370 348 341 284 226 170 152 138 AseI AT'TA_AT Cuts at: 160 1032 5752 160 Size: 872 4720 1606 Fragments arranged by size: 4720 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 AvaI C'yCGr_G Cuts at: 1870 1943 2595 2775 4523 1870 Size: 73 652 180 1748 4545 Fragments arranged by size: 4545 1748 652 180 73 BamHI G'GATC_C Cuts at: 2188 4528 4895 2188 Size: 2340 367 4491 Fragments arranged by size: 4491 2340 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2958 3144 4519 5974 618 Size: 325 217 627 1171 186 1375 1455 1842 Fragments arranged by size: 1842 1455 1375 1171 627 325 217 186 BanII G_rGCy'C Cuts at: 1391 2385 2676 3261 3757 3964 4517 1391 Size: 994 291 585 496 207 553 4072 Fragments arranged by size: 4072 994 585 553 496 291 207 BbeI G_GCGC'C Cuts at: 3148 3148 Size: 7198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3033 928 Size: 971 1134 5093 Fragments arranged by size: 5093 1134 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5389 5423 1903 Size: 34 3452 34 3678 Fragments arranged by size: 3678 3452 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5090 6300 5090 Size: 1210 5988 BclI T'GATC_A Cuts at: 1548 1548 Size: 7198 BfrBI ATG'CAT Cuts at: 3802 3802 Size: 7198 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5809 136 Size: 107 122 71 631 605 4137 1525 Fragments arranged by size: 4137 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7193 7193 Size: 7198 BlpI GC'TnA_GC Cuts at: 3260 4615 3260 Size: 1355 5843 Bme1580I G_kGCm'C Cuts at: 1790 4440 5259 6415 1790 Size: 2650 819 1156 2573 Fragments arranged by size: 2650 2573 1156 819 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3538 4929 5887 Size: 416 53 83 399 24 2121 1391 958 Cuts at: 5887 442 Size: 1753 Fragments arranged by size: 2121 1753 1391 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2345 1056 Size: 1289 5909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 3954 4164 4332 5858 1239 Size: 866 1849 210 168 1526 2579 Fragments arranged by size: 2579 1849 1526 866 210 168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2656 2667 4142 5323 6188 6486 2656 Size: 11 1475 1181 865 298 3368 Fragments arranged by size: 3368 1475 1181 865 298 11 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3326 5861 882 Size: 617 1827 2535 2219 Fragments arranged by size: 2535 2219 1827 617 BsaAI yAC'GTr Cuts at: 493 1204 2105 2170 4495 4989 493 Size: 711 901 65 2325 494 2702 Fragments arranged by size: 2702 2325 901 711 494 65 BsaBI GATnn'nnATC Cuts at: 7192 7192 Size: 7198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3145 5005 5387 6022 Size: 53 83 186 936 1612 1860 382 635 Cuts at: 6022 275 Size: 1451 Fragments arranged by size: 1860 1612 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2151 5631 6029 6303 6450 6861 2151 Size: 3480 398 274 147 411 2488 Fragments arranged by size: 3480 2488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6973 Fragments arranged by size: 6973 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3744 4058 4274 4285 1186 Size: 2558 314 216 11 4099 Fragments arranged by size: 4099 2558 314 216 11 BseYI C'CCAG_C Cuts at: 2426 2988 6401 6897 2426 Size: 562 3413 496 2727 Fragments arranged by size: 3413 2727 562 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2280 4365 1163 Size: 573 544 2085 3996 Fragments arranged by size: 3996 2085 573 544 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4648 5409 6437 1540 Size: 275 186 2647 761 1028 2301 Fragments arranged by size: 2647 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2649 2676 3261 3757 3964 4440 5259 5344 6415 Size: 27 585 496 207 476 819 85 1071 Cuts at: 6415 2649 Size: 3432 Fragments arranged by size: 3432 1071 819 585 496 476 207 85 27 BsmI GAATG_Cn' Cuts at: 2063 2770 3240 4709 4802 2063 Size: 707 470 1469 93 4459 Fragments arranged by size: 4459 1469 707 470 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3450 3450 Size: 7198 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2649 2676 3261 3757 3964 4440 Size: 399 595 264 27 585 496 207 476 Cuts at: 4440 4517 5259 5344 6415 1391 Size: 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 585 496 476 399 264 207 85 77 27 BspEI T'CCGG_A Cuts at: 6861 6861 Size: 7198 BspHI T'CATG_A Cuts at: 2668 3948 4218 5087 6817 2668 Size: 1280 270 869 1730 3049 Fragments arranged by size: 3049 1730 1280 869 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2970 3367 4553 844 Size: 2126 397 1186 3489 Fragments arranged by size: 3489 2126 1186 397 BsrBI CCG'CTC Cuts at: 1394 1823 3303 3656 3768 4355 5085 1394 Size: 429 1480 353 112 587 730 3507 Fragments arranged by size: 3507 1480 730 587 429 353 112 BsrDI GCAATG_nn' Cuts at: 65 1248 2887 5693 5867 65 Size: 1183 1639 2806 174 1396 Fragments arranged by size: 2806 1639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2994 5842 6029 1700 Size: 110 1184 2848 187 2869 Fragments arranged by size: 2869 2848 1184 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7198 BssSI C'ACGA_G Cuts at: 3695 5258 6270 3695 Size: 1563 1012 4623 Fragments arranged by size: 4623 1563 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4444 794 Size: 3650 3548 BstBI TT'CG_AA Cuts at: 2007 6002 2007 Size: 3995 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7198 BstYI r'GATC_y Cuts at: 1972 2188 4528 4895 5280 5297 6749 6835 6847 Size: 216 2340 367 385 17 1452 86 12 Cuts at: 6847 7193 1972 Size: 346 1977 Fragments arranged by size: 2340 1977 1452 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2954 513 Size: 552 1889 4757 Fragments arranged by size: 4757 1889 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6459 Fragments arranged by size: 6459 683 56 BtsI GCAGTG_nn' Cuts at: 2352 3003 4714 5508 5528 2352 Size: 651 1711 794 20 4022 Fragments arranged by size: 4022 1711 794 651 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7198 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2992 3027 529 Size: 35 2428 35 4700 Fragments arranged by size: 4700 2428 35 35 DraI TTT'AAA Cuts at: 4002 4502 4002 Size: 500 6698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2843 6205 809 Size: 507 1527 3362 1802 Fragments arranged by size: 3362 1802 1527 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2996 4026 4455 Size: 54 1425 50 275 18 1166 1030 429 Cuts at: 4455 4645 5534 7163 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1425 1166 1030 889 429 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4645 1537 Size: 275 2833 4090 Fragments arranged by size: 4090 2833 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2989 3602 3897 4425 5128 1353 Size: 1332 304 613 295 528 703 3423 Fragments arranged by size: 3423 1332 703 613 528 304 295 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5785 6159 6305 387 Size: 1064 138 4196 374 146 1280 Fragments arranged by size: 4196 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2674 3259 3755 3962 2674 Size: 585 496 207 5910 Fragments arranged by size: 5910 585 496 207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2360 2877 3149 3434 3954 4164 Size: 393 473 255 517 272 285 520 210 Cuts at: 4164 4262 4332 5261 5858 6645 6913 1239 Size: 98 70 929 597 787 268 1524 Fragments arranged by size: 1524 929 787 597 520 517 473 393 285 272 268 255 210 98 70 EcoNI CCTnn'n_nnAGG Cuts at: 2852 2852 Size: 7198 EcoO109I rG'GnC_Cy Cuts at: 2224 3536 2224 Size: 1312 5886 EcoRI G'AATT_C Cuts at: 3833 4507 4885 3833 Size: 674 378 6146 Fragments arranged by size: 6146 674 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2104 3370 2104 Size: 1266 5932 FspI TGC'GCA Cuts at: 2420 5704 2420 Size: 3284 3914 HaeII r_GCGC'y Cuts at: 1678 2143 2995 3148 6345 1678 Size: 465 852 153 3197 2531 Fragments arranged by size: 3197 2531 852 465 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 2310 2342 3014 Size: 32 135 32 608 32 98 32 672 Cuts at: 3014 3020 3046 3052 4200 4232 5839 5871 5913 Size: 6 26 6 1148 32 1607 32 42 Cuts at: 5913 5945 1373 Size: 32 2626 Fragments arranged by size: 2626 1607 1148 672 608 135 98 42 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3013 4542 4788 669 Size: 988 281 1075 1529 246 3079 Fragments arranged by size: 3079 1529 1075 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 748 Size: 1702 5496 HpaI GTT'AAC Cuts at: 4788 4788 Size: 7198 KasI G'GCGC_C Cuts at: 3144 3144 Size: 7198 KpnI G_GTAC'C Cuts at: 4523 4523 Size: 7198 MfeI C'AATT_G Cuts at: 3926 4797 3926 Size: 871 6327 MluI A'CGCG_T Cuts at: 7074 7074 Size: 7198 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 2754 3417 4382 6312 6496 1305 Size: 1110 339 663 965 1930 184 2007 Fragments arranged by size: 2007 1930 1110 965 663 339 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4028 4457 10 Size: 54 1425 343 2196 429 2751 Fragments arranged by size: 2751 2196 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2975 3224 4154 4387 5156 5515 5674 Size: 648 1809 249 930 233 769 359 159 Cuts at: 5674 518 Size: 2042 Fragments arranged by size: 2042 1809 930 769 648 359 249 233 159 MspA1I CmG'CkG Cuts at: 1606 3264 4402 4453 4614 5291 6439 6684 1606 Size: 1658 1138 51 161 677 1148 245 2120 Fragments arranged by size: 2120 1658 1148 1138 677 245 161 51 NaeI GCC'GGC Cuts at: 2996 2996 Size: 7198 NarI GG'CG_CC Cuts at: 3145 3145 Size: 7198 NcoI C'CATG_G Cuts at: 513 1065 2954 513 Size: 552 1889 4757 Fragments arranged by size: 4757 1889 552 NdeI CA'TA_TG Cuts at: 387 3384 4417 387 Size: 2997 1033 3168 Fragments arranged by size: 3168 2997 1033 NgoMIV G'CCGG_C Cuts at: 2994 2994 Size: 7198 NheI G'CTAG_C Cuts at: 1052 2341 1052 Size: 1289 5909 NotI GC'GGCC_GC Cuts at: 4645 4645 Size: 7198 NsiI A_TGCA'T Cuts at: 3804 3804 Size: 7198 NspI r_CATG'y Cuts at: 1132 1229 3431 4392 4489 4556 6101 1132 Size: 97 2202 961 97 67 1545 2229 Fragments arranged by size: 2229 2202 1545 961 97 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7198 PciI A'CATG_T Cuts at: 4388 6097 4388 Size: 1709 5489 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 6058 PfoI T'CCnGG_A Cuts at: 3169 3507 3169 Size: 338 6860 PmeI GTTT'AAAC Cuts at: 4502 4502 Size: 7198 PmlI CAC'GTG Cuts at: 2105 2105 Size: 7198 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5267 5299 6780 6812 5267 Size: 32 1481 32 5653 Fragments arranged by size: 5653 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2224 Size: 7198 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7198 PsiI TTA'TAA Cuts at: 2928 4768 2928 Size: 1840 5358 PstI C_TGCA'G Cuts at: 830 4452 4550 830 Size: 3622 98 3478 Fragments arranged by size: 3622 3478 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7198 PvuII CAG'CTG Cuts at: 3264 4402 4453 3264 Size: 1138 51 6009 Fragments arranged by size: 6009 1138 51 SacI G_AGCT'C Cuts at: 2676 3261 3757 3964 2676 Size: 585 496 207 5910 Fragments arranged by size: 5910 585 496 207 SalI G'TCGA_C Cuts at: 1936 4540 1936 Size: 2604 4594 SapI GCTCTTCn'nnn_ Cuts at: 3602 3602 Size: 7198 SbfI CC_TGCA'GG Cuts at: 4550 4550 Size: 7198 ScaI AGT'ACT Cuts at: 2698 3812 5446 7158 2698 Size: 1114 1634 1712 2738 Fragments arranged by size: 2738 1712 1634 1114 SfcI C'TryA_G Cuts at: 826 1045 2941 4448 4546 5681 6362 6553 826 Size: 219 1896 1507 98 1135 681 191 1471 Fragments arranged by size: 1896 1507 1471 1135 681 219 191 98 SfoI GGC'GCC Cuts at: 3146 3146 Size: 7198 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7198 SmaI CCC'GGG Cuts at: 1872 4525 1872 Size: 2653 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2635 2682 2775 4121 5302 6203 Size: 197 926 692 47 93 1346 1181 901 Cuts at: 6203 6465 820 Size: 262 1553 Fragments arranged by size: 1553 1346 1181 926 901 692 262 197 93 47 SnaBI TAC'GTA Cuts at: 493 4495 4989 493 Size: 4002 494 2702 Fragments arranged by size: 4002 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7198 SphI G_CATG'C Cuts at: 1132 4489 4556 1132 Size: 3357 67 3774 Fragments arranged by size: 3774 3357 67 SspI AAT'ATT Cuts at: 5 52 5122 5 Size: 47 5070 2081 Fragments arranged by size: 5070 2081 47 StuI AGG'CCT Cuts at: 1517 4068 1517 Size: 2551 4647 StyI C'CwwG_G Cuts at: 513 1065 2954 3190 4637 513 Size: 552 1889 236 1447 3074 Fragments arranged by size: 3074 1889 1447 552 236 SwaI ATTT'AAAT Cuts at: 4002 4002 Size: 7198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2983 5225 5242 5395 5580 6025 325 Size: 1255 1403 2242 17 153 185 445 1498 Fragments arranged by size: 2242 1498 1403 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2696 3081 3810 Size: 274 80 33 51 1443 719 385 729 Cuts at: 3810 5444 7156 96 Size: 1634 1712 138 Fragments arranged by size: 1712 1634 1443 729 719 385 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2034 2066 2451 2483 3681 3713 7158 7190 2034 Size: 32 385 32 1198 32 3445 32 2042 Fragments arranged by size: 3445 2042 1198 385 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3203 1316 Size: 1887 5311 XbaI T'CTAG_A Cuts at: 4534 4534 Size: 7198 XhoI C'TCGA_G Cuts at: 1943 2775 1943 Size: 832 6366 XmaI C'CCGG_G Cuts at: 1870 4523 1870 Size: 2653 4545 XmnI GAAnn'nnTTC Cuts at: 2373 3119 3356 5327 2373 Size: 746 237 1971 4244 Fragments arranged by size: 4244 1971 746 237 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5006 276 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI XcmI