Restriction map for
pFN21ASDA0317
(Linear) (Six-base) MAPSORT of: KIAA0317.seq Check: 3766 from: 1 to: 2469
KIAA0317.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 947 2469
Size: 947 1522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 854 1126 1411 2239 2469
Size: 337 517 272 285 828 230
Fragments arranged by size:
828 517 337 285 272 230
AflIII A'CryG_T
Cuts at: 0 81 2365 2469
Size: 81 2284 104
Fragments arranged by size:
2284 104 81
AleI CACnn'nnGTG
Cuts at: 0 1201 2469
Size: 1201 1268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 473 505 2469
Size: 281 32 160 32 1964
Fragments arranged by size:
1964 281 160 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 31 2469
Size: 31 2438
ApaLI G'TGCA_C
Cuts at: 0 2413 2469
Size: 2413 56
ApoI r'AATT_y
Cuts at: 0 329 467 808 1156 1440 1810 1980 2469
Size: 329 138 341 348 284 370 170 489
Fragments arranged by size:
489 370 348 341 329 284 170 138
AvaI C'yCGr_G
Cuts at: 0 572 752 2469
Size: 572 180 1717
Fragments arranged by size:
1717 572 180
BamHI G'GATC_C
Cuts at: 0 165 2469
Size: 165 2304
BanI G'GyrC_C
Cuts at: 0 935 1121 2469
Size: 935 186 1348
Fragments arranged by size:
1348 935 186
BanII G_rGCy'C
Cuts at: 0 362 653 1238 1734 1941 2469
Size: 362 291 585 496 207 528
Fragments arranged by size:
585 528 496 362 291 207
BbeI G_GCGC'C
Cuts at: 0 1125 2469
Size: 1125 1344
BbsI GAAGACnn'nnnn_
Cuts at: 0 1010 2469
Size: 1010 1459
BfrBI ATG'CAT
Cuts at: 0 1779 2469
Size: 1779 690
BlpI GC'TnA_GC
Cuts at: 0 1237 2469
Size: 1237 1232
Bme1580I G_kGCm'C
Cuts at: 0 2417 2469
Size: 2417 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1515 2469
Size: 1515 954
BmtI G_CTAG'C
Cuts at: 0 322 2469
Size: 322 2147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1931 2141 2309 2469
Size: 82 1849 210 168 160
Fragments arranged by size:
1849 210 168 160 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 644 2119 2469
Size: 633 11 1475 350
Fragments arranged by size:
1475 633 350 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 1303 2469
Size: 1303 1166
BsaAI yAC'GTr
Cuts at: 0 82 147 2469
Size: 82 65 2322
Fragments arranged by size:
2322 82 65
BsaHI Gr'CG_yC
Cuts at: 0 1122 2469
Size: 1122 1347
BsaWI w'CCGG_w
Cuts at: 0 128 2469
Size: 128 2341
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1721 2035 2251 2262 2469
Size: 1721 314 216 11 207
Fragments arranged by size:
1721 314 216 207 11
BseYI C'CCAG_C
Cuts at: 0 403 965 2469
Size: 403 562 1504
Fragments arranged by size:
1504 562 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 2342 2469
Size: 257 2085 127
Fragments arranged by size:
2085 257 127
BsiHKAI G_wGCw'C
Cuts at: 0 626 653 1238 1734 1941 2417 2469
Size: 626 27 585 496 207 476 52
Fragments arranged by size:
626 585 496 476 207 52 27
BsmI GAATG_Cn'
Cuts at: 0 40 747 1217 2469
Size: 40 707 470 1252
Fragments arranged by size:
1252 707 470 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1427 2469
Size: 1427 1042
Bsp1286I G_dGCh'C
Cuts at: 0 362 626 653 1238 1734 1941 2417 2469
Size: 362 264 27 585 496 207 476 52
Fragments arranged by size:
585 496 476 362 264 207 52 27
BspHI T'CATG_A
Cuts at: 0 645 1925 2195 2469
Size: 645 1280 270 274
Fragments arranged by size:
1280 645 274 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 1344 2469
Size: 947 397 1125
Fragments arranged by size:
1125 947 397
BsrBI CCG'CTC
Cuts at: 0 1280 1633 1745 2332 2469
Size: 1280 353 112 587 137
Fragments arranged by size:
1280 587 353 137 112
BsrDI GCAATG_nn'
Cuts at: 0 864 2469
Size: 864 1605
BsrFI r'CCGG_y
Cuts at: 0 971 2469
Size: 971 1498
BssSI C'ACGA_G
Cuts at: 0 1672 2469
Size: 1672 797
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2421 2469
Size: 2421 48
BstYI r'GATC_y
Cuts at: 0 165 2469
Size: 165 2304
BtgI C'CryG_G
Cuts at: 0 931 2469
Size: 931 1538
BtsI GCAGTG_nn'
Cuts at: 0 329 980 2469
Size: 329 651 1489
Fragments arranged by size:
1489 651 329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 969 1004 2469
Size: 969 35 1465
Fragments arranged by size:
1465 969 35
DraI TTT'AAA
Cuts at: 0 1979 2469
Size: 1979 490
DrdI GACnn_nn'nnGTC
Cuts at: 0 820 2469
Size: 820 1649
EaeI y'GGCC_r
Cuts at: 0 973 2003 2432 2469
Size: 973 1030 429 37
Fragments arranged by size:
1030 973 429 37
EarI CTCTTCn'nnn_
Cuts at: 0 662 966 1579 1874 2402 2469
Size: 662 304 613 295 528 67
Fragments arranged by size:
662 613 528 304 295 67
EcoICRI GAG'CTC
Cuts at: 0 651 1236 1732 1939 2469
Size: 651 585 496 207 530
Fragments arranged by size:
651 585 530 496 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 337 854 1126 1411 1931 2141 2239
Size: 82 255 517 272 285 520 210 98
Cuts at: 2239 2309 2469
Size: 70 160
Fragments arranged by size:
520 517 285 272 255 210 160 98
82 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 829 2469
Size: 829 1640
EcoO109I rG'GnC_Cy
Cuts at: 0 201 1513 2469
Size: 201 1312 956
Fragments arranged by size:
1312 956 201
EcoRI G'AATT_C
Cuts at: 0 1810 2469
Size: 1810 659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 1347 2469
Size: 81 1266 1122
Fragments arranged by size:
1266 1122 81
FspI TGC'GCA
Cuts at: 0 397 2469
Size: 397 2072
HaeII r_GCGC'y
Cuts at: 0 120 972 1125 2469
Size: 120 852 153 1344
Fragments arranged by size:
1344 852 153 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 287 319 991 997 1023 1029
Size: 157 32 98 32 672 6 26 6
Cuts at: 1029 2177 2209 2469
Size: 1148 32 260
Fragments arranged by size:
1148 672 260 157 98 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 990 2469
Size: 990 1479
HindIII A'AGCT_T
Cuts at: 0 427 2469
Size: 427 2042
KasI G'GCGC_C
Cuts at: 0 1121 2469
Size: 1121 1348
MfeI C'AATT_G
Cuts at: 0 1903 2469
Size: 1903 566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 731 1394 2359 2469
Size: 392 339 663 965 110
Fragments arranged by size:
965 663 392 339 110
MscI TGG'CCA
Cuts at: 0 2005 2434 2469
Size: 2005 429 35
Fragments arranged by size:
2005 429 35
MslI CAynn'nnrTG
Cuts at: 0 952 1201 2131 2364 2469
Size: 952 249 930 233 105
Fragments arranged by size:
952 930 249 233 105
MspA1I CmG'CkG
Cuts at: 0 1241 2379 2430 2469
Size: 1241 1138 51 39
Fragments arranged by size:
1241 1138 51 39
NaeI GCC'GGC
Cuts at: 0 973 2469
Size: 973 1496
NarI GG'CG_CC
Cuts at: 0 1122 2469
Size: 1122 1347
NcoI C'CATG_G
Cuts at: 0 931 2469
Size: 931 1538
NdeI CA'TA_TG
Cuts at: 0 1361 2394 2469
Size: 1361 1033 75
Fragments arranged by size:
1361 1033 75
NgoMIV G'CCGG_C
Cuts at: 0 971 2469
Size: 971 1498
NheI G'CTAG_C
Cuts at: 0 318 2469
Size: 318 2151
NsiI A_TGCA'T
Cuts at: 0 1781 2469
Size: 1781 688
NspI r_CATG'y
Cuts at: 0 1408 2369 2466 2469
Size: 1408 961 97 3
Fragments arranged by size:
1408 961 97 3
PciI A'CATG_T
Cuts at: 0 2365 2469
Size: 2365 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 2469
Size: 225 2244
PfoI T'CCnGG_A
Cuts at: 0 1146 1484 2469
Size: 1146 338 985
Fragments arranged by size:
1146 985 338
PmlI CAC'GTG
Cuts at: 0 82 2469
Size: 82 2387
PpuMI rG'GwC_Cy
Cuts at: 0 201 2469
Size: 201 2268
PsiI TTA'TAA
Cuts at: 0 905 2469
Size: 905 1564
PstI C_TGCA'G
Cuts at: 0 2429 2469
Size: 2429 40
PvuII CAG'CTG
Cuts at: 0 1241 2379 2430 2469
Size: 1241 1138 51 39
Fragments arranged by size:
1241 1138 51 39
SacI G_AGCT'C
Cuts at: 0 653 1238 1734 1941 2469
Size: 653 585 496 207 528
Fragments arranged by size:
653 585 528 496 207
SapI GCTCTTCn'nnn_
Cuts at: 0 1579 2469
Size: 1579 890
ScaI AGT'ACT
Cuts at: 0 675 1789 2469
Size: 675 1114 680
Fragments arranged by size:
1114 680 675
SfcI C'TryA_G
Cuts at: 0 918 2425 2469
Size: 918 1507 44
Fragments arranged by size:
1507 918 44
SfoI GGC'GCC
Cuts at: 0 1123 2469
Size: 1123 1346
SmlI C'TyrA_G
Cuts at: 0 612 659 752 2098 2469
Size: 612 47 93 1346 371
Fragments arranged by size:
1346 612 371 93 47
SphI G_CATG'C
Cuts at: 0 2466 2469
Size: 2466 3
StuI AGG'CCT
Cuts at: 0 2045 2469
Size: 2045 424
StyI C'CwwG_G
Cuts at: 0 931 1167 2469
Size: 931 236 1302
Fragments arranged by size:
1302 931 236
SwaI ATTT'AAAT
Cuts at: 0 1979 2469
Size: 1979 490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 960 2469
Size: 960 1509
TatI w'GTAC_w
Cuts at: 0 673 1058 1787 2469
Size: 673 385 729 682
Fragments arranged by size:
729 682 673 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 428 460 1658 1690 2469
Size: 11 32 385 32 1198 32 779
Fragments arranged by size:
1198 779 385 32 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 0 1180 2469
Size: 1180 1289
XhoI C'TCGA_G
Cuts at: 0 752 2469
Size: 752 1717
XmnI GAAnn'nnTTC
Cuts at: 0 350 1096 1333 2469
Size: 350 746 237 1136
Fragments arranged by size:
1136 746 350 237
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BfrBI BlpI
Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI
BtgI BtsI CspCI DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmlI PpuMI PsiI PstI PvuII
SacI SapI ScaI SfcI SfoI SmlI SphI StuI
StyI SwaI TaqII TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BglI BglII BmgBI BplI Bpu10I
BsaBI BsaXI BsiEI BsiWI BspEI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII EagI
EciI EcoRV FseI FspAI HpaI KpnI MluI NotI
NruI PacI PasI PmeI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
XbaI XcmI XmaI ZraI