(Circular) (Six-base) MAPSORT of: pFN21ASDA0315.seq Check: 279 from: 1 to: 10243 pFN21ASDA0315 10243 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2060 2209 2864 3135 4371 6985 2060 Size: 149 655 271 1236 2614 5318 Fragments arranged by size: 5318 2614 1236 655 271 149 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4653 6620 8053 278 Size: 53 83 186 936 3117 1967 1433 2468 Fragments arranged by size: 3117 2468 1967 1433 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5673 6066 7586 1937 Size: 3736 393 1520 4594 Fragments arranged by size: 4594 3736 1520 393 Acc65I G'GTAC_C Cuts at: 7564 7564 Size: 10243 AclI AA'CG_TT Cuts at: 1563 4573 8370 8743 1563 Size: 3010 3797 373 3063 Fragments arranged by size: 3797 3063 3010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2896 5029 8306 9690 9958 1632 Size: 1264 2133 3277 1384 268 1917 Fragments arranged by size: 3277 2133 1917 1384 1264 268 AfeI AGC'GCT Cuts at: 5429 5429 Size: 10243 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10046 AflIII A'CryG_T Cuts at: 1203 5157 9142 10119 1203 Size: 3954 3985 977 1327 Fragments arranged by size: 3985 3954 1327 977 AgeI A'CCGG_T Cuts at: 9074 9074 Size: 10243 AhdI GACnn_n'nnGTC Cuts at: 6616 8972 6616 Size: 2356 7887 AleI CACnn'nnGTG Cuts at: 1166 2767 3170 3203 5597 7385 1166 Size: 1601 403 33 2394 1788 4024 Fragments arranged by size: 4024 2394 1788 1601 403 33 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2785 3188 4154 5858 7253 7518 1184 Size: 1601 403 966 1704 1395 265 3909 Fragments arranged by size: 3909 1704 1601 1395 966 403 265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3389 3421 3389 Size: 32 10211 AlwNI CAG_nnn'CTG Cuts at: 2320 3549 4030 4320 6598 6742 7986 9558 2320 Size: 1229 481 290 2278 144 1244 1572 3005 Fragments arranged by size: 3005 2278 1572 1244 1229 481 290 144 ApaI G_GGCC'C Cuts at: 2044 2234 2943 3622 4463 6329 2044 Size: 190 709 679 841 1866 5958 Fragments arranged by size: 5958 1866 841 709 679 190 ApaLI G'TGCA_C Cuts at: 2254 2978 5822 8300 9456 2254 Size: 724 2844 2478 1156 3041 Fragments arranged by size: 3041 2844 2478 1156 724 ApoI r'AATT_y Cuts at: 1493 5777 7552 7778 7930 10039 1493 Size: 4284 1775 226 152 2109 1697 Fragments arranged by size: 4284 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8797 160 Size: 872 7765 1606 Fragments arranged by size: 7765 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10243 AvaI C'yCGr_G Cuts at: 1870 1943 3024 5364 5534 7356 7568 1870 Size: 73 1081 2340 170 1822 212 4545 Fragments arranged by size: 4545 2340 1822 1081 212 170 73 BamHI G'GATC_C Cuts at: 3264 4443 7296 7456 7573 7940 3264 Size: 1179 2853 160 117 367 5567 Fragments arranged by size: 5567 2853 1179 367 160 117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2852 3020 3248 3293 Size: 325 217 627 285 780 168 228 45 Cuts at: 3293 4751 4772 5253 5369 5420 6789 6955 6970 Size: 1458 21 481 116 51 1369 166 15 Cuts at: 6970 7564 9019 618 Size: 594 1455 1842 Fragments arranged by size: 1842 1458 1455 1369 780 627 594 481 325 285 228 217 168 166 116 51 45 21 15 BanII G_rGCy'C Cuts at: 1391 2044 2234 2498 2943 3095 3159 3622 4463 Size: 653 190 264 445 152 64 463 841 Cuts at: 4463 4793 5088 5475 6150 6282 6329 6584 7562 Size: 330 295 387 675 132 47 255 978 Cuts at: 7562 1391 Size: 4072 Fragments arranged by size: 4072 978 841 675 653 463 445 387 330 295 264 255 190 152 132 64 47 BbeI G_GCGC'C Cuts at: 6959 6959 Size: 10243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2698 2707 2784 2902 4645 5176 5447 Size: 971 799 9 77 118 1743 531 271 Cuts at: 5447 5866 7047 7067 928 Size: 419 1181 20 4104 Fragments arranged by size: 4104 1743 1181 971 799 531 419 271 118 77 20 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 5377 5411 8434 8468 1903 Size: 34 2312 34 1094 34 3023 34 3678 Fragments arranged by size: 3678 3023 2312 1094 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8135 9345 8135 Size: 1210 9033 BclI T'GATC_A Cuts at: 1548 5913 1548 Size: 4365 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2064 2873 3539 Size: 107 122 71 631 605 392 809 666 Cuts at: 3539 5500 5947 8854 136 Size: 1961 447 2907 1525 Fragments arranged by size: 2907 1961 1525 809 666 631 605 447 392 122 107 71 BglII A'GATC_T Cuts at: 6879 7254 10238 6879 Size: 375 2984 6884 Fragments arranged by size: 6884 2984 375 BlpI GC'TnA_GC Cuts at: 5615 7211 7660 5615 Size: 1596 449 8198 Fragments arranged by size: 8198 1596 449 Bme1580I G_kGCm'C Cuts at: 1790 2044 2234 2258 2943 2982 3251 3622 4003 Size: 254 190 24 685 39 269 371 381 Cuts at: 4003 4424 4463 4935 5256 5263 5372 5646 5826 Size: 421 39 472 321 7 109 274 180 Cuts at: 5826 6329 6914 6966 8304 9460 1790 Size: 503 585 52 1338 1156 2573 Fragments arranged by size: 2573 1338 1156 685 585 503 472 421 381 371 321 274 269 254 190 180 109 52 39 39 24 7 BmgBI CAC'GTC Cuts at: 1318 3840 4804 5608 5821 5908 7126 1318 Size: 2522 964 804 213 87 1218 4435 Fragments arranged by size: 4435 2522 1218 964 804 213 87 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3445 4018 4673 Size: 416 53 83 399 24 2028 573 655 Cuts at: 4673 7974 8932 442 Size: 3301 958 1753 Fragments arranged by size: 3301 2028 1753 958 655 573 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10243 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3328 6704 3328 Size: 3376 6867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2822 3079 3384 3905 4844 4925 5704 Size: 992 591 257 305 521 939 81 779 Cuts at: 5704 5756 5765 6050 6128 6164 6173 6587 6788 Size: 52 9 285 78 36 9 414 201 Cuts at: 6788 7535 8903 1239 Size: 747 1368 2579 Fragments arranged by size: 2579 1368 992 939 779 747 591 521 414 305 285 257 201 81 78 52 36 9 9 Bpu10I CC'TnA_GC Cuts at: 2396 3431 3662 5937 2396 Size: 1035 231 2275 6702 Fragments arranged by size: 6702 2275 1035 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3254 4127 6245 7085 8368 9233 9531 3254 Size: 873 2118 840 1283 865 298 3966 Fragments arranged by size: 3966 2118 1283 873 865 840 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3360 4915 5447 6715 8906 882 Size: 617 1861 1555 532 1268 2191 2219 Fragments arranged by size: 2219 2191 1861 1555 1268 617 532 BsaAI yAC'GTr Cuts at: 493 1204 3790 4126 5158 5296 6163 7540 8034 Size: 711 2586 336 1032 138 867 1377 494 Cuts at: 8034 493 Size: 2702 Fragments arranged by size: 2702 2586 1377 1032 867 711 494 336 138 BsaBI GATnn'nnATC Cuts at: 6239 10237 6239 Size: 3998 6245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4509 4622 4650 4824 Size: 53 83 186 936 2976 113 28 174 Cuts at: 4824 5331 6447 6574 6617 6956 7149 8050 8432 Size: 507 1116 127 43 339 193 901 382 Cuts at: 8432 9067 275 Size: 635 1451 Fragments arranged by size: 2976 1451 1116 936 901 635 507 382 339 193 186 174 127 113 83 53 43 28 BsaWI w'CCGG_w Cuts at: 2589 7080 8676 9074 9348 9495 9906 2589 Size: 4491 1596 398 274 147 411 2926 Fragments arranged by size: 4491 2926 1596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 3662 3692 4286 Size: 30 165 30 1374 30 855 30 594 Cuts at: 4286 4316 4397 4427 4775 4805 5963 5993 6269 Size: 30 81 30 348 30 1158 30 276 Cuts at: 6269 6299 6467 6497 6620 6650 7355 7385 1178 Size: 30 168 30 123 30 705 30 4036 Fragments arranged by size: 4036 1374 1158 855 705 594 348 276 168 165 123 81 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3289 3551 3683 3800 3902 5222 5762 6785 Size: 2103 262 132 117 102 1320 540 1023 Cuts at: 6785 6788 7466 1186 Size: 3 678 3963 Fragments arranged by size: 3963 2103 1320 1023 678 540 262 132 117 102 3 BseYI C'CCAG_C Cuts at: 2053 2062 3139 3594 4037 5572 5730 6382 6714 Size: 9 1077 455 443 1535 158 652 332 Cuts at: 6714 7226 9446 9942 2053 Size: 512 2220 496 2354 Fragments arranged by size: 2354 2220 1535 1077 652 512 496 455 443 332 158 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2791 3430 3440 3653 4106 4220 5416 Size: 573 1055 639 10 213 453 114 1196 Cuts at: 5416 5504 6080 6449 7174 7331 7494 1163 Size: 88 576 369 725 157 163 3912 Fragments arranged by size: 3912 1196 1055 725 639 576 573 453 369 213 163 157 114 88 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3204 3213 4549 5869 5951 6803 Size: 275 186 1203 9 1336 1320 82 852 Cuts at: 6803 6807 7693 8454 9482 1540 Size: 4 886 761 1028 2301 Fragments arranged by size: 2301 1336 1320 1203 1028 886 852 761 275 186 82 9 4 BsiHKAI G_wGCw'C Cuts at: 2258 2498 2576 2982 3126 5826 6150 8304 8389 Size: 240 78 406 144 2700 324 2154 85 Cuts at: 8389 9460 2258 Size: 1071 3041 Fragments arranged by size: 3041 2700 2154 1071 406 324 240 144 85 78 BsmI GAATG_Cn' Cuts at: 2182 7754 7847 2182 Size: 5572 93 4578 Fragments arranged by size: 5572 4578 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4217 4448 4613 4217 Size: 231 165 9847 Fragments arranged by size: 9847 231 165 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2234 2258 2498 2576 2943 2982 Size: 399 254 190 24 240 78 367 39 Cuts at: 2982 3095 3126 3159 3251 3622 4003 4424 4463 Size: 113 31 33 92 371 381 421 39 Cuts at: 4463 4793 4935 5088 5256 5263 5372 5475 5646 Size: 330 142 153 168 7 109 103 171 Cuts at: 5646 5826 6150 6282 6329 6584 6914 6966 7562 Size: 180 324 132 47 255 330 52 596 Cuts at: 7562 8304 8389 9460 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 596 421 399 381 371 367 330 330 324 255 254 240 190 180 171 168 153 142 132 113 109 103 92 85 78 52 47 39 39 33 31 24 7 BspEI T'CCGG_A Cuts at: 9906 9906 Size: 10243 BspHI T'CATG_A Cuts at: 8132 9862 8132 Size: 1730 8513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2060 2209 2820 2864 3135 3452 3903 3938 Size: 1216 149 611 44 271 317 451 35 Cuts at: 3938 3951 4371 5332 5538 5994 6186 6750 6985 Size: 13 420 961 206 456 192 564 235 Cuts at: 6985 7303 7598 844 Size: 318 295 3489 Fragments arranged by size: 3489 1216 961 611 564 456 451 420 318 317 295 271 235 206 192 149 44 35 13 BsrBI CCG'CTC Cuts at: 1394 1823 5614 6859 7078 8130 1394 Size: 429 3791 1245 219 1052 3507 Fragments arranged by size: 3791 3507 1245 1052 429 219 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 2548 8738 8912 65 Size: 1183 1219 81 6190 174 1396 Fragments arranged by size: 6190 1396 1219 1183 174 81 BsrFI r'CCGG_y Cuts at: 1700 1810 2645 3415 3612 4156 4544 5831 5918 Size: 110 835 770 197 544 388 1287 87 Cuts at: 5918 5950 6633 8887 9074 1700 Size: 32 683 2254 187 2869 Fragments arranged by size: 2869 2254 1287 835 770 683 544 388 197 187 110 87 32 BsrGI T'GTAC_A Cuts at: 96 6285 96 Size: 6189 4054 BssHII G'CGCG_C Cuts at: 1925 2389 1925 Size: 464 9779 BssSI C'ACGA_G Cuts at: 3953 4064 5825 7136 7385 8303 9315 3953 Size: 111 1761 1311 249 918 1012 4881 Fragments arranged by size: 4881 1761 1311 1012 918 249 111 BstAPI GCAn_nnn'nTGC Cuts at: 794 3453 3573 3909 4320 4329 6742 6796 6970 Size: 2659 120 336 411 9 2413 54 174 Cuts at: 6970 7309 794 Size: 339 3728 Fragments arranged by size: 3728 2659 2413 411 339 336 174 120 54 9 BstBI TT'CG_AA Cuts at: 2007 9047 2007 Size: 7040 3203 BstEII G'GTnAC_C Cuts at: 1184 2513 4159 4810 1184 Size: 1329 1646 651 6617 Fragments arranged by size: 6617 1646 1329 651 BstXI CCAn_nnnn'nTGG Cuts at: 3883 4045 5140 3883 Size: 162 1095 8986 Fragments arranged by size: 8986 1095 162 BstYI r'GATC_y Cuts at: 1972 3264 4443 4533 6594 6879 7254 7296 7456 Size: 1292 1179 90 2061 285 375 42 160 Cuts at: 7456 7573 7940 8325 8342 9794 9880 9892 10238 Size: 117 367 385 17 1452 86 12 346 Cuts at: 10238 1972 Size: 1977 Fragments arranged by size: 2061 1977 1452 1292 1179 385 375 367 346 285 160 117 90 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2081 2149 3981 5205 2081 Size: 68 1832 1224 7119 Fragments arranged by size: 7119 1832 1224 68 BtgI C'CryG_G Cuts at: 513 1065 2022 2226 2499 2871 3058 3527 4768 Size: 552 957 204 273 372 187 469 1241 Cuts at: 4768 5067 5108 5646 5942 6520 513 Size: 299 41 538 296 578 4236 Fragments arranged by size: 4236 1241 957 578 552 538 469 372 299 296 273 204 187 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5062 5402 5644 7150 487 Size: 683 56 3836 340 242 1506 3580 Fragments arranged by size: 3836 3580 1506 683 340 242 56 BtsI GCAGTG_nn' Cuts at: 2027 2822 2938 4138 6914 7759 8553 8573 2027 Size: 795 116 1200 2776 845 794 20 3697 Fragments arranged by size: 3697 2776 1200 845 795 794 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10208 DraI TTT'AAA Cuts at: 7547 7547 Size: 10243 DraIII CAC_nnn'GTG Cuts at: 3453 6796 6970 7141 3453 Size: 3343 174 171 6555 Fragments arranged by size: 6555 3343 174 171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3992 5177 6560 7130 9250 809 Size: 507 2676 1185 1383 570 2120 1802 Fragments arranged by size: 2676 2120 1802 1383 1185 570 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2454 2475 Size: 54 1425 50 275 18 393 231 21 Cuts at: 2475 2862 3079 3201 3259 3579 3792 4309 4542 Size: 387 217 122 58 320 213 517 233 Cuts at: 4542 4546 5014 5948 6168 6800 7690 8579 10208 Size: 4 468 934 220 632 890 889 1629 Cuts at: 10208 8 Size: 43 Fragments arranged by size: 1629 1425 934 890 889 632 517 468 393 387 320 275 233 231 220 217 213 122 58 54 50 43 21 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3201 4546 5948 6800 7690 1537 Size: 275 1389 1345 1402 852 890 4090 Fragments arranged by size: 4090 1402 1389 1345 890 852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 5684 6143 6299 8173 1353 Size: 1019 3312 459 156 1874 3423 Fragments arranged by size: 3423 3312 1874 1019 459 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2402 7478 8830 9204 9350 387 Size: 1064 138 813 5076 1352 374 146 1280 Fragments arranged by size: 5076 1352 1280 1064 813 374 146 138 EcoICRI GAG'CTC Cuts at: 2496 6148 2496 Size: 3652 6591 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2822 2896 3079 3384 3905 4844 Size: 393 599 591 74 183 305 521 939 Cuts at: 4844 4925 5029 5704 5756 5765 6050 6128 6164 Size: 81 104 675 52 9 285 78 36 Cuts at: 6164 6173 6587 6788 7535 8306 8903 9690 9958 Size: 9 414 201 747 771 597 787 268 Cuts at: 9958 1239 Size: 1524 Fragments arranged by size: 1524 939 787 771 747 675 599 597 591 521 414 393 305 285 268 201 183 104 81 78 74 52 36 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2369 3665 6698 6767 6770 2369 Size: 1296 3033 69 3 5842 Fragments arranged by size: 5842 3033 1296 69 3 EcoO109I rG'GnC_Cy Cuts at: 2041 2085 2504 2824 2939 3469 3940 4034 4179 Size: 44 419 320 115 530 471 94 145 Cuts at: 4179 4396 4460 4923 5283 5740 5965 6325 6463 Size: 217 64 463 360 457 225 360 138 Cuts at: 6463 6739 2041 Size: 276 5545 Fragments arranged by size: 5545 530 471 463 457 419 360 360 320 276 225 217 145 138 115 94 64 44 EcoRI G'AATT_C Cuts at: 5777 7552 7930 5777 Size: 1775 378 8090 Fragments arranged by size: 8090 1775 378 EcoRV GAT'ATC Cuts at: 3067 3067 Size: 10243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 5200 6085 7009 2116 Size: 3084 885 924 5350 Fragments arranged by size: 5350 3084 924 885 FseI GG_CCGG'CC Cuts at: 4548 4548 Size: 10243 FspI TGC'GCA Cuts at: 6194 8749 6194 Size: 2555 7688 HaeII r_GCGC'y Cuts at: 1678 2347 3594 5431 6095 6757 6959 9390 1678 Size: 669 1247 1837 664 662 202 2431 2531 Fragments arranged by size: 2531 2431 1837 1247 669 664 662 202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 3281 3313 3813 Size: 32 135 32 560 32 1117 32 500 Cuts at: 3813 3845 4775 4807 5105 5137 6467 6499 6602 Size: 32 930 32 298 32 1330 32 103 Cuts at: 6602 6634 6812 6844 8884 8916 8958 8990 1373 Size: 32 178 32 2040 32 42 32 2626 Fragments arranged by size: 2626 2040 1330 1117 930 560 500 298 178 135 103 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2311 5347 6649 7587 7833 669 Size: 988 281 373 3036 1302 938 246 3079 Fragments arranged by size: 3079 3036 1302 988 938 373 281 246 HindIII A'AGCT_T Cuts at: 748 4999 748 Size: 4251 5992 HpaI GTT'AAC Cuts at: 7833 7833 Size: 10243 KasI G'GCGC_C Cuts at: 6955 6955 Size: 10243 KpnI G_GTAC'C Cuts at: 7568 7568 Size: 10243 MfeI C'AATT_G Cuts at: 7842 7842 Size: 10243 MluI A'CGCG_T Cuts at: 10119 10119 Size: 10243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 4723 6006 6252 6582 9357 9541 1305 Size: 1260 2158 1283 246 330 2775 184 2007 Fragments arranged by size: 2775 2158 2007 1283 1260 330 246 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 2456 2477 6170 10 Size: 54 1425 343 393 231 21 3693 4083 Fragments arranged by size: 4083 3693 1425 393 343 231 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2567 2767 3170 3203 5138 5597 5663 Size: 648 1401 200 403 33 1935 459 66 Cuts at: 5663 5840 6455 6656 6666 7385 8201 8560 8719 Size: 177 615 201 10 719 816 359 159 Cuts at: 8719 518 Size: 2042 Fragments arranged by size: 2042 1935 1401 816 719 648 615 459 403 359 201 200 177 159 66 33 10 MspA1I CmG'CkG Cuts at: 1606 2191 2209 2317 2436 2843 2968 3139 3529 Size: 585 18 108 119 407 125 171 390 Cuts at: 3529 3637 3924 4017 5110 5632 5734 5766 5944 Size: 108 287 93 1093 522 102 32 178 Cuts at: 5944 6040 6625 7480 7508 7659 8336 9484 9729 Size: 96 585 855 28 151 677 1148 245 Cuts at: 9729 1606 Size: 2120 Fragments arranged by size: 2120 1148 1093 855 677 585 585 522 407 390 287 245 178 171 151 125 119 108 108 102 96 93 32 28 18 NaeI GCC'GGC Cuts at: 2647 3614 4546 5833 2647 Size: 967 932 1287 7057 Fragments arranged by size: 7057 1287 967 932 NarI GG'CG_CC Cuts at: 6956 6956 Size: 10243 NcoI C'CATG_G Cuts at: 513 1065 2022 5067 5646 513 Size: 552 957 3045 579 5110 Fragments arranged by size: 5110 3045 957 579 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10243 NgoMIV G'CCGG_C Cuts at: 2645 3612 4544 5831 2645 Size: 967 932 1287 7057 Fragments arranged by size: 7057 1287 967 932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10243 NotI GC'GGCC_GC Cuts at: 7690 7690 Size: 10243 NruI TCG'CGA Cuts at: 5562 5562 Size: 10243 NspI r_CATG'y Cuts at: 1132 1229 2770 7133 7601 9146 1132 Size: 97 1541 4363 468 1545 2229 Fragments arranged by size: 4363 2229 1545 1541 468 97 PasI CC'CwG_GG Cuts at: 1348 3622 4465 1348 Size: 2274 843 7126 Fragments arranged by size: 7126 2274 843 PciI A'CATG_T Cuts at: 9142 9142 Size: 10243 PflMI CCAn_nnn'nTGG Cuts at: 1108 3549 4030 6028 7141 1108 Size: 2441 481 1998 1113 4210 Fragments arranged by size: 4210 2441 1998 1113 481 PfoI T'CCnGG_A Cuts at: 6141 6150 6564 6672 6765 7002 7032 6141 Size: 9 414 108 93 237 30 9352 Fragments arranged by size: 9352 414 237 108 93 30 9 PmeI GTTT'AAAC Cuts at: 7547 7547 Size: 10243 PmlI CAC'GTG Cuts at: 3790 4126 3790 Size: 336 9907 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8312 8344 9825 9857 8312 Size: 32 1481 32 8698 Fragments arranged by size: 8698 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2504 2824 3469 3940 4034 4179 4923 6463 6739 Size: 320 645 471 94 145 744 1540 276 Cuts at: 6739 2504 Size: 6008 Fragments arranged by size: 6008 1540 744 645 471 320 276 145 94 PshAI GACnn'nnGTC Cuts at: 1613 4017 1613 Size: 2404 7839 PsiI TTA'TAA Cuts at: 7813 7813 Size: 10243 PspOMI G'GGCC_C Cuts at: 2040 2230 2939 3618 4459 6325 2040 Size: 190 709 679 841 1866 5958 Fragments arranged by size: 5958 1866 841 709 679 190 PstI C_TGCA'G Cuts at: 830 2034 2208 2817 3180 4325 5094 5130 5400 Size: 1204 174 609 363 1145 769 36 270 Cuts at: 5400 6921 6982 7497 7595 830 Size: 1521 61 515 98 3478 Fragments arranged by size: 3478 1521 1204 1145 769 609 515 363 270 174 98 61 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10243 PvuII CAG'CTG Cuts at: 2191 2209 2317 3637 4017 5734 7480 2191 Size: 18 108 1320 380 1717 1746 4954 Fragments arranged by size: 4954 1746 1717 1320 380 108 18 SacI G_AGCT'C Cuts at: 2498 6150 2498 Size: 3652 6591 SacII CC_GC'GG Cuts at: 3530 5111 5945 3530 Size: 1581 834 7828 Fragments arranged by size: 7828 1581 834 SalI G'TCGA_C Cuts at: 1936 7585 1936 Size: 5649 4594 SanDI GG'GwC_CC Cuts at: 2824 4034 4923 2824 Size: 1210 889 8144 Fragments arranged by size: 8144 1210 889 SapI GCTCTTCn'nnn_ Cuts at: 5684 6143 6299 5684 Size: 459 156 9628 Fragments arranged by size: 9628 459 156 SbfI CC_TGCA'GG Cuts at: 7595 7595 Size: 10243 ScaI AGT'ACT Cuts at: 6113 6989 7424 8491 10203 6113 Size: 876 435 1067 1712 6153 Fragments arranged by size: 6153 1712 1067 876 435 SexAI A'CCwGG_T Cuts at: 3351 6510 6792 3351 Size: 3159 282 6802 Fragments arranged by size: 6802 3159 282 SfcI C'TryA_G Cuts at: 826 1045 2030 2204 2813 3176 4321 4351 5090 Size: 219 985 174 609 363 1145 30 739 Cuts at: 5090 5126 5396 6917 6978 7493 7591 8726 9407 Size: 36 270 1521 61 515 98 1135 681 Cuts at: 9407 9598 826 Size: 191 1471 Fragments arranged by size: 1521 1471 1145 1135 985 739 681 609 515 363 270 219 191 174 98 61 36 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3539 3539 Size: 10243 SfoI GGC'GCC Cuts at: 6957 6957 Size: 10243 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10243 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10243 SmaI CCC'GGG Cuts at: 1872 3026 5366 7358 7570 1872 Size: 1154 2340 1992 212 4545 Fragments arranged by size: 4545 2340 1992 1154 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 3269 4142 6260 7100 8347 9248 Size: 197 926 1326 873 2118 840 1247 901 Cuts at: 9248 9510 820 Size: 262 1553 Fragments arranged by size: 2118 1553 1326 1247 926 901 873 840 262 197 SnaBI TAC'GTA Cuts at: 493 7540 8034 493 Size: 7047 494 2702 Fragments arranged by size: 7047 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10243 SphI G_CATG'C Cuts at: 1132 7601 1132 Size: 6469 3774 SspI AAT'ATT Cuts at: 5 52 8167 5 Size: 47 8115 2081 Fragments arranged by size: 8115 2081 47 StuI AGG'CCT Cuts at: 1517 2147 2609 3189 5219 5411 1517 Size: 630 462 580 2030 192 6349 Fragments arranged by size: 6349 2030 630 580 462 192 StyI C'CwwG_G Cuts at: 513 1065 2022 5067 5175 5646 5889 6060 6699 Size: 552 957 3045 108 471 243 171 639 Cuts at: 6699 7248 7446 7682 513 Size: 549 198 236 3074 Fragments arranged by size: 3074 3045 957 639 552 549 471 243 236 198 171 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3464 4457 4790 5408 5471 5557 6821 Size: 1255 1884 993 333 618 63 86 1264 Cuts at: 6821 7078 8270 8287 8440 8625 9070 325 Size: 257 1192 17 153 185 445 1498 Fragments arranged by size: 1884 1498 1264 1255 1192 993 618 445 333 257 185 153 86 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5463 6111 6285 Size: 274 80 33 51 1443 3486 648 174 Cuts at: 6285 6357 6987 7422 8489 10201 96 Size: 72 630 435 1067 1712 138 Fragments arranged by size: 3486 1712 1443 1067 648 630 435 274 174 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 4585 4617 4749 4781 6620 6652 7355 Size: 32 1752 32 132 32 1839 32 703 Cuts at: 7355 7387 10203 10235 2801 Size: 32 2816 32 2809 Fragments arranged by size: 2816 2809 1839 1752 703 132 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2306 5870 1316 Size: 990 3564 5689 Fragments arranged by size: 5689 3564 990 XbaI T'CTAG_A Cuts at: 7579 7579 Size: 10243 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 2485 4868 5140 6518 2344 Size: 141 2383 272 1378 6069 Fragments arranged by size: 6069 2383 1378 272 141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10243 XmaI C'CCGG_G Cuts at: 1870 3024 5364 7356 7568 1870 Size: 1154 2340 1992 212 4545 Fragments arranged by size: 4545 2340 1992 1154 212 XmnI GAAnn'nnTTC Cuts at: 2447 5777 8372 2447 Size: 3330 2595 4318 Fragments arranged by size: 4318 3330 2595 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4651 6618 8051 276 Size: 53 83 186 936 3117 1967 1433 2468 Fragments arranged by size: 3117 2468 1967 1433 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I FspAI NsiI PacI PspXI PsrI RsrII SrfI SwaI