Restriction map for
pFN21ASDA0313
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# NO CUT ENZYMES ( 82 enzymes) #
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AccIII AclI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspHI AviII BbrPI
BbvCI BcgI BciVI BfrI BsaAI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I FseI
FspI KspI MluI MroI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI SspBI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 3747 GACGTC GACGT^C
Acc65I 820 GGTACC G^GTACC
AccI 2662 GTAGAC GT^[AC][GT]AC
Asp718I 820 GGTACC G^GTACC
AspI 417 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3549 CCTAGG C^CTAGG
BalI 1311 TGGCCA TGG^CCA
BamHI 3849 GGATCC G^GATCC
BbeI 3512 GGCGCC GGCGC^C
BlnI 3549 CCTAGG C^CTAGG
BlpI 2145 GCTCAGC GC^T[ACGT]AGC
BpmI 3065 CTCCAG CTGGAG(16/14)
Bpu1102I 2145 GCTCAGC GC^T[ACGT]AGC
BsaWI 350 ACCGGA [AT]^CCGG[AT]
BspHI 1865 TCATGA T^CATGA
BstAPI 390 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 111 GGTGACC G^GT[ACGT]ACC
BstPI 111 GGTGACC G^GT[ACGT]ACC
CelII 2145 GCTCAGC GC^T[ACGT]AGC
DraIII 1952 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2665 GACGGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 111 GGTGACC G^GT[ACGT]ACC
EcoRI 4223 GAATTC G^AATTC
EcoRV 181 GATATC GAT^ATC
HaeII 3512 GGCGCC [GA]GCGC^[CT]
KasI 3512 GGCGCC G^GCGCC
KpnI 820 GGTACC GGTAC^C
MfeI 344 CAATTG C^AATTG
MluNI 1311 TGGCCA TGG^CCA
MscI 1311 TGGCCA TGG^CCA
MunI 344 CAATTG C^AATTG
NarI 3512 GGCGCC GG^CGCC
NdeI 3880 CATATG CA^TATG
NspI 722 ACATGC [GA]CATG^[CT]
PflFI 417 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3220 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1865 TCATGA T^CATGA
SbfI 3853 CCTGCAGG CCTGCA^GG
ScaI 4147 AGTACT AGT^ACT
SfoI 3512 GGCGCC GGC^GCC
SpeI 151 ACTAGT A^CTAGT
Sse8387I 3853 CCTGCAGG CCTGCA^GG
SspI 1946 AATATT AAT^ATT
StuI 4381 AGGCCT AGG^CCT
Tth111I 417 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3220 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3398 CCAGTGGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 3103 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3422 CAGATTCTG
BanII 4159 GGGCTC G[GA]GC[CT]^C
4192 GAGCCC
BcnI 1938 CCCGG CC^[GC]GG
4210 CCCGG
BglI 2911 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4356 GCCACATGGGC
BglII 1509 AGATCT A^GATCT
2514 AGATCT
BsaBI 1623 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2352 GATGAAGATC
BsgI 2386 CTGCAC GTGCAG(16/14)
3499 CTGCAC
BspMI 3856 GCAGGT ACCTGC(4/8)
4181 GCAGGT
BstXI 1571 CCAACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2540 CCAGAAACATGG
DraII 338 AGGACCC [GA]G^G[ACGT]CC[CT]
3607 GGGTCCT
EcoNI 330 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
824 CCTCAGAAAGG
EcoO109I 338 AGGACCC [GA]G^G[ACGT]CC[CT]
3607 GGGTCCT
EcoT22I 841 ATGCAT ATGCA^T
3326 ATGCAT
HgaI 3709 GCGTC GACGC(5/10)
3979 GCGTC
HindIII 2896 AAGCTT A^AGCTT
3245 AAGCTT
HpaI 834 GTTAAC GTT^AAC
1161 GTTAAC
MamI 1623 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2352 GATGAAGATC
MslI 1018 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4131 CATAGCTGTG
NciI 1938 CCCGG CC^[GC]GG
4210 CCCGG
NcoI 27 CCATGG C^CATGG
611 CCATGG
NsiI 841 ATGCAT ATGCA^T
3326 ATGCAT
PpuMI 338 AGGACCC [GA]G^G[AT]CC[CT]
3607 GGGTCCT
SapI 502 GAAGAGC GCTCTTC(1/4)
853 GAAGAGC
SmlI 1673 CTCAAG C^T[CT][GA]AG
3756 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AocI ApoI
Asp700I AspEI AvaI AvaII BanI
BbsI BbvI BclI BfaI BmyI
BpuAI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI Eam1105I EarI
Eco81I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI