(Circular) (Six-base) MAPSORT of: pFN21ASDA0277.seq Check: 2612 from: 1 to: 6469 pFN21ASDA0277 6469 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2970 3370 3812 1937 Size: 1033 400 442 4594 Fragments arranged by size: 4594 1033 442 400 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2897 4532 5916 6184 1632 Size: 900 365 1635 1384 268 1917 Fragments arranged by size: 1917 1635 1384 900 365 268 AflII C'TTAA_G Cuts at: 820 1017 2699 820 Size: 197 1682 4590 Fragments arranged by size: 4590 1682 197 AflIII A'CryG_T Cuts at: 1203 2760 5368 6345 1203 Size: 1557 2608 977 1327 Fragments arranged by size: 2608 1557 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2418 3278 1184 Size: 1234 860 4375 Fragments arranged by size: 4375 1234 860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 2161 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 2386 3611 3727 4212 5784 2386 Size: 1225 116 485 1572 3071 Fragments arranged by size: 3071 1572 1225 485 116 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 2606 2748 3085 3278 3322 3778 4004 4156 Size: 1113 142 337 193 44 456 226 152 Cuts at: 4156 6265 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1113 456 337 226 193 152 142 44 AseI AT'TA_AT Cuts at: 160 1032 3080 5023 160 Size: 872 2048 1943 1606 Fragments arranged by size: 2048 1943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2945 3756 3794 1870 Size: 73 1002 811 38 4545 Fragments arranged by size: 4545 1002 811 73 38 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3790 5245 618 Size: 325 217 627 2003 1455 1842 Fragments arranged by size: 2003 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3738 3788 1391 Size: 2347 50 4072 Fragments arranged by size: 4072 2347 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2347 928 Size: 971 279 169 5050 Fragments arranged by size: 5050 971 279 169 BbvCI CC'TCA_GC Cuts at: 2039 2039 Size: 6469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BfrBI ATG'CAT Cuts at: 3476 3476 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3252 5080 136 Size: 107 122 71 631 605 1580 1828 1525 Fragments arranged by size: 1828 1580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 4530 5686 1790 Size: 2740 1156 2573 Fragments arranged by size: 2740 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 4200 5158 Size: 416 53 83 399 24 1056 1727 958 Cuts at: 5158 442 Size: 1753 Fragments arranged by size: 1753 1727 1056 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2449 2218 Size: 231 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 3176 5129 1239 Size: 1460 477 1953 2579 Fragments arranged by size: 2579 1953 1460 477 Bpu10I CC'TnA_GC Cuts at: 2039 2039 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4594 5459 5757 4594 Size: 865 298 5306 Fragments arranged by size: 5306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 3353 5132 882 Size: 617 813 1041 1779 2219 Fragments arranged by size: 2219 1779 1041 813 617 BsaAI yAC'GTr Cuts at: 493 1204 2346 2761 3766 4260 493 Size: 711 1142 415 1005 494 2702 Fragments arranged by size: 2702 1142 1005 711 494 415 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4276 4658 5293 275 Size: 53 83 186 936 2743 382 635 1451 Fragments arranged by size: 2743 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2973 4902 5300 5574 5721 6132 2973 Size: 1929 398 274 147 411 3310 Fragments arranged by size: 3310 1929 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 1178 Size: 30 165 30 758 30 5456 Fragments arranged by size: 5456 758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 1186 Size: 976 5493 BseYI C'CCAG_C Cuts at: 3250 5672 6168 3250 Size: 2422 496 3551 Fragments arranged by size: 3551 2422 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 1163 Size: 573 1548 4348 Fragments arranged by size: 4348 1548 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 2778 3738 4530 4615 5686 2271 Size: 507 960 792 85 1071 3054 Fragments arranged by size: 3054 1071 960 792 507 85 BsmI GAATG_Cn' Cuts at: 2631 3476 3980 4073 2631 Size: 845 504 93 5027 Fragments arranged by size: 5027 845 504 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2778 3738 3788 4530 4615 5686 Size: 399 481 507 960 50 742 85 1071 Cuts at: 5686 1391 Size: 2174 Fragments arranged by size: 2174 1071 960 742 507 481 399 85 50 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 2358 3544 4358 6088 2358 Size: 1186 814 1730 2739 Fragments arranged by size: 2739 1730 1186 814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3824 844 Size: 2980 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3208 4964 5138 65 Size: 1183 1960 1756 174 1396 Fragments arranged by size: 1960 1756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2493 96 Size: 2397 4072 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 3116 4529 5541 3116 Size: 1413 1012 4044 Fragments arranged by size: 4044 1413 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 2007 5273 2007 Size: 3266 3203 BstEII G'GTnAC_C Cuts at: 1184 2505 3650 1184 Size: 1321 1145 4003 Fragments arranged by size: 4003 1321 1145 BstXI CCAn_nnnn'nTGG Cuts at: 3343 3343 Size: 6469 BstYI r'GATC_y Cuts at: 1972 2823 2847 2866 3064 3442 3799 4166 4551 Size: 851 24 19 198 378 357 367 385 Cuts at: 4551 4568 6020 6106 6118 6464 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 851 385 378 367 357 346 198 86 24 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2079 3225 513 Size: 552 957 57 1146 3757 Fragments arranged by size: 3757 1146 957 552 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 3168 3298 3354 3628 3985 4779 4799 3168 Size: 130 56 274 357 794 20 4838 Fragments arranged by size: 4838 794 357 274 130 56 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 2538 3773 2538 Size: 1235 5234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2880 3222 3916 Size: 54 1425 50 275 18 1050 342 694 Cuts at: 3916 4805 6434 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1050 889 694 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2856 4399 1353 Size: 1503 1543 3423 Fragments arranged by size: 3423 1543 1503 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 3002 5056 5430 5576 387 Size: 1064 138 476 937 2054 374 146 1280 Fragments arranged by size: 2054 1280 1064 937 476 374 146 138 EcoICRI GAG'CTC Cuts at: 3736 3736 Size: 6469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2699 2897 3176 4532 5129 5916 Size: 393 900 167 198 279 1356 597 787 Cuts at: 5916 6184 1239 Size: 268 1524 Fragments arranged by size: 1524 1356 900 787 597 393 279 268 198 167 EcoO109I rG'GnC_Cy Cuts at: 2247 2247 Size: 6469 EcoRI G'AATT_C Cuts at: 3322 3778 4156 3322 Size: 456 378 5635 Fragments arranged by size: 5635 456 378 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 2087 5616 1678 Size: 409 3529 2531 Fragments arranged by size: 3529 2531 409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3668 3700 3739 3771 5110 Size: 32 135 32 2096 32 39 32 1339 Cuts at: 5110 5142 5184 5216 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2096 1339 135 42 39 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3371 3813 4059 669 Size: 988 281 1433 442 246 3079 Fragments arranged by size: 3079 1433 988 442 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5583 5767 1305 Size: 4278 184 2007 Fragments arranged by size: 4278 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 3224 10 Size: 54 1425 343 1050 342 3255 Fragments arranged by size: 3255 1425 1050 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2781 3341 3702 4427 4786 4945 518 Size: 648 1615 560 361 725 359 159 2042 Fragments arranged by size: 2042 1615 725 648 560 361 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 3250 3268 3885 4562 5710 5955 1606 Size: 475 1169 18 617 677 1148 245 2120 Fragments arranged by size: 2120 1169 1148 677 617 475 245 18 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4960 Fragments arranged by size: 4960 957 552 NdeI CA'TA_TG Cuts at: 387 3597 387 Size: 3210 3259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NsiI A_TGCA'T Cuts at: 3478 3478 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 3827 5372 1132 Size: 97 2598 1545 2229 Fragments arranged by size: 2598 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3398 1348 Size: 2050 4419 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PfoI T'CCnGG_A Cuts at: 2243 2301 2325 2243 Size: 58 24 6387 Fragments arranged by size: 6387 58 24 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PmlI CAC'GTG Cuts at: 2761 2761 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2161 2193 4538 4570 6051 6083 2161 Size: 32 2345 32 1481 32 2547 Fragments arranged by size: 2547 2345 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2247 Size: 6469 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspXI vC'TCGA_Gb Cuts at: 2945 2945 Size: 6469 PstI C_TGCA'G Cuts at: 830 3245 3635 3821 830 Size: 2415 390 186 3478 Fragments arranged by size: 3478 2415 390 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 3250 3268 3250 Size: 18 6451 SacI G_AGCT'C Cuts at: 3738 3738 Size: 6469 SacII CC_GC'GG Cuts at: 2082 2082 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3369 3811 1936 Size: 1433 442 4594 Fragments arranged by size: 4594 1433 442 SanDI GG'GwC_CC Cuts at: 2247 2247 Size: 6469 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 2643 3241 3466 3631 3817 4952 5633 Size: 219 1598 598 225 165 186 1135 681 Cuts at: 5633 5824 826 Size: 191 1471 Fragments arranged by size: 1598 1471 1135 681 598 225 219 191 186 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 2945 4573 5474 5736 820 Size: 197 926 756 246 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 756 262 246 197 SnaBI TAC'GTA Cuts at: 493 3766 4260 493 Size: 3273 494 2702 Fragments arranged by size: 3273 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 2654 4393 5 Size: 47 2602 1739 2081 Fragments arranged by size: 2602 2081 1739 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2022 3908 513 Size: 552 957 1886 3074 Fragments arranged by size: 3074 1886 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2109 3208 4496 4513 4666 4851 5296 Size: 1255 529 1099 1288 17 153 185 445 Cuts at: 5296 325 Size: 1498 Fragments arranged by size: 1498 1288 1255 1099 529 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2493 2557 4715 Size: 274 80 33 51 1443 516 64 2158 Cuts at: 4715 6427 96 Size: 1712 138 Fragments arranged by size: 2158 1712 1443 516 274 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3486 3518 6429 6461 3486 Size: 32 2911 32 3494 Fragments arranged by size: 3494 2911 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2927 3611 1316 Size: 1611 684 4174 Fragments arranged by size: 4174 1611 684 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2945 1943 Size: 1002 5467 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 3278 3326 3416 4598 3278 Size: 48 90 1182 5149 Fragments arranged by size: 5149 1182 90 48 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI