Restriction map for
pFN21ASDA0272
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 562 GTCTAC GT^[AC][GT]AC
AccII 1550 CGCG CG^CG
AcyI 2099 GGCGCC G[GA]^CG[CT]C
AflIII 236 ACATGT A^C[GA][CT]GT
AspHI 295 GTGCTC G[AT]GC[AT]^C
AvaI 439 CCCGAG C^[CT]CG[GA]G
AviII 958 TGCGCA TGC^GCA
BbeI 2099 GGCGCC GGCGC^C
BbvCI 1693 GCTGAGG CCTCAGC(-5/-2)
BglI 2142 GCCTGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 92 AGATCT A^GATCT
BsaHI 2099 GGCGCC G[GA]^CG[CT]C
BsaI 183 GGTCTC GGTCTC(1/5)
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
BsoBI 439 CCCGAG C^[CT]CG[GA]G
BspLU11I 236 ACATGT A^CATGT
BspMI 1135 GCAGGT ACCTGC(4/8)
BssSI 52 CTCGTG CACGAG(-5/-1)
BstAPI 274 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1550 CGCG CG^CG
BstYI 92 AGATCT [GA]^GATC[CT]
DrdI 816 GACCCACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2118 CGGCCG C^GGCCG
EclXI 2118 CGGCCG C^GGCCG
Eco52I 2118 CGGCCG C^GGCCG
FspI 958 TGCGCA TGC^GCA
HgaI 1548 GACGC GACGC(5/10)
Hin1I 2099 GGCGCC G[GA]^CG[CT]C
HincII 1621 GTTGAC GT[CT]^[GA]AC
HindII 1621 GTTGAC GT[CT]^[GA]AC
KasI 2099 GGCGCC G^GCGCC
MflI 92 AGATCT [GA]^GATC[CT]
MvnI 1550 CGCG CG^CG
NarI 2099 GGCGCC GG^CGCC
SapI 160 GAAGAGC GCTCTTC(1/4)
SfaNI 2114 GCATC GCATC(5/9)
SfoI 2099 GGCGCC GGC^GCC
SphI 2057 GCATGC GCATG^C
TaqI 158 TCGA T^CGA
TfiI 810 GATTC G^A[AT]TC
TthHB8I 158 TCGA T^CGA
XbaI 1080 TCTAGA T^CTAGA
XhoII 92 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 155 GGATC GGATC(4/5)
713 GGATC
AocI 844 CCTGAGG CC^T[ACGT]AGG
1245 CCTTAGG
Asp700I 345 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1971 GAAGAAGTTC
BalI 410 TGGCCA TGG^CCA
2063 TGGCCA
BanI 699 GGTGCC G^G[CT][GA]CC
2099 GGCGCC
BbsI 1123 GAAGAC GAAGAC(2/6)
1833 GAAGAC
BlpI 288 GCTGAGC GC^T[ACGT]AGC
1535 GCTCAGC
Bpu1102I 288 GCTGAGC GC^T[ACGT]AGC
1535 GCTCAGC
BpuAI 1123 GAAGAC GAAGAC(2/6)
1833 GAAGAC
BsiEI 705 CGACCG CG[GA][CT]^CG
2118 CGGCCG
BsmAI 184 GTCTC GTCTC(1/5)
825 GTCTC
BsrBI 1533 CCGCTC CCGCTC(-3/-3)
1287 GAGCGG
BsrDI 395 GCAATG GCAATG(2/0)
1466 GCAATG
BsrI 1159 CCAGT ACTGG(1/-1)
1792 CCAGT
Bsu36I 844 CCTGAGG CC^T[ACGT]AGG
1245 CCTTAGG
CelII 288 GCTGAGC GC^T[ACGT]AGC
1535 GCTCAGC
Eco81I 844 CCTGAGG CC^T[ACGT]AGG
1245 CCTTAGG
MluNI 410 TGGCCA TGG^CCA
2063 TGGCCA
MscI 410 TGGCCA TGG^CCA
2063 TGGCCA
NcoI 491 CCATGG C^CATGG
576 CCATGG
ScaI 762 AGTACT AGT^ACT
1178 AGTACT
Tsp45I 834 GTCAC ^GT[GC]AC
1567 GTCAC
Tsp509I 1093 AATT ^AATT
1380 AATT
XmnI 345 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1971 GAAGAAGTTC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SmlI StuI
StyI TseI TspRI XcmI