(Circular) (Six-base) MAPSORT of: pFN21ASDA0272.seq Check: 5693 from: 1 to: 6916 pFN21ASDA0272 6916 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3149 3149 Size: 6916 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4726 278 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2586 4259 1937 Size: 649 1673 4594 Fragments arranged by size: 4594 1673 649 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 5043 5416 1563 Size: 3480 373 3063 Fragments arranged by size: 3480 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2600 2786 3950 4979 6363 6631 1632 Size: 479 489 186 1164 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1164 1029 489 479 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6719 AflIII A'CryG_T Cuts at: 1203 2259 5815 6792 1203 Size: 1056 3556 977 1327 Fragments arranged by size: 3556 1327 1056 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3719 1184 Size: 2535 4381 AlwNI CAG_nnn'CTG Cuts at: 2806 2936 3091 4659 6231 2806 Size: 130 155 1568 1572 3491 Fragments arranged by size: 3491 1572 1568 155 130 ApaI G_GGCC'C Cuts at: 2147 2607 2926 2147 Size: 460 319 6137 Fragments arranged by size: 6137 460 319 ApaLI G'TGCA_C Cuts at: 4973 6129 4973 Size: 1156 5760 ApoI r'AATT_y Cuts at: 1493 4225 4451 4603 6712 1493 Size: 2732 226 152 2109 1697 Fragments arranged by size: 2732 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5470 160 Size: 872 4438 1606 Fragments arranged by size: 4438 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 2462 4241 1870 Size: 73 519 1779 4545 Fragments arranged by size: 4545 1779 519 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2217 2250 2217 Size: 33 6883 BamHI G'GATC_C Cuts at: 4246 4613 4246 Size: 367 6549 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2722 4122 4237 5692 618 Size: 325 217 627 935 1400 115 1455 1842 Fragments arranged by size: 1842 1455 1400 935 627 325 217 115 BanII G_rGCy'C Cuts at: 1391 2147 2607 2688 2926 3885 4235 1391 Size: 756 460 81 238 959 350 4072 Fragments arranged by size: 4072 959 756 460 350 238 81 BbeI G_GCGC'C Cuts at: 4126 4126 Size: 6916 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3153 3863 928 Size: 971 1254 710 3981 Fragments arranged by size: 3981 1254 971 710 BbvCI CC'TCA_GC Cuts at: 3717 3717 Size: 6916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5107 5141 1903 Size: 34 3170 34 3678 Fragments arranged by size: 3678 3170 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4808 6018 4808 Size: 1210 5706 BclI T'GATC_A Cuts at: 1548 1548 Size: 6916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 5527 136 Size: 107 122 71 631 605 2499 1356 1525 Fragments arranged by size: 2499 1525 1356 631 605 122 107 71 BglII A'GATC_T Cuts at: 2115 6911 2115 Size: 4796 2120 BlpI GC'TnA_GC Cuts at: 2312 3559 4333 2312 Size: 1247 774 4895 Fragments arranged by size: 4895 1247 774 Bme1580I G_kGCm'C Cuts at: 1790 2147 2607 2727 2926 3459 4977 6133 1790 Size: 357 460 120 199 533 1518 1156 2573 Fragments arranged by size: 2573 1518 1156 533 460 357 199 120 BmgBI CAC'GTC Cuts at: 1318 3247 1318 Size: 1929 4987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3809 4647 5605 Size: 416 53 83 399 24 2392 838 958 Cuts at: 5605 442 Size: 1753 Fragments arranged by size: 2392 1753 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3919 3919 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2066 3021 3565 5576 1239 Size: 821 6 955 544 2011 2579 Fragments arranged by size: 2579 2011 955 821 544 6 Bpu10I CC'TnA_GC Cuts at: 3717 4071 3717 Size: 354 6562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2835 3907 3956 5041 5906 6204 2835 Size: 1072 49 1085 865 298 3547 Fragments arranged by size: 3547 1085 1072 865 298 49 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2212 5579 882 Size: 617 713 3367 2219 Fragments arranged by size: 3367 2219 713 617 BsaAI yAC'GTr Cuts at: 493 1204 4213 4707 493 Size: 711 3009 494 2702 Fragments arranged by size: 3009 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6910 6910 Size: 6916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4123 4723 5105 5740 Size: 53 83 186 936 2590 600 382 635 Cuts at: 5740 275 Size: 1451 Fragments arranged by size: 2590 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5349 5747 6021 6168 6579 5349 Size: 398 274 147 411 5686 Fragments arranged by size: 5686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2460 2490 2864 2894 1178 Size: 30 165 30 1057 30 374 30 5200 Fragments arranged by size: 5200 1057 374 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2636 2885 2978 3251 3992 1186 Size: 940 510 249 93 273 741 4110 Fragments arranged by size: 4110 940 741 510 273 249 93 BseYI C'CCAG_C Cuts at: 3195 3434 3485 6119 6615 3195 Size: 239 51 2634 496 3496 Fragments arranged by size: 3496 2634 496 239 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2523 2979 3269 3692 4033 1163 Size: 573 787 456 290 423 341 4046 Fragments arranged by size: 4046 787 573 456 423 341 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 4144 4366 5127 6155 1540 Size: 275 186 730 1413 222 761 1028 2301 Fragments arranged by size: 2301 1413 1028 761 730 275 222 186 BsiHKAI G_wGCw'C Cuts at: 2322 4977 5062 6133 2322 Size: 2655 85 1071 3105 Fragments arranged by size: 3105 2655 1071 85 BsmI GAATG_Cn' Cuts at: 4427 4520 4427 Size: 93 6823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2322 2607 2688 2727 2926 3459 Size: 399 357 175 285 81 39 199 533 Cuts at: 3459 3885 4235 4977 5062 6133 1391 Size: 426 350 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 533 426 399 357 350 285 199 175 85 81 39 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 4805 6535 4805 Size: 1730 5186 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3149 4271 844 Size: 2305 1122 3489 Fragments arranged by size: 3489 2305 1122 BsrBI CCG'CTC Cuts at: 1394 1823 3312 3558 4803 1394 Size: 429 1489 246 1245 3507 Fragments arranged by size: 3507 1489 1245 429 246 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 3496 5411 5585 65 Size: 1183 1177 1071 1915 174 1396 Fragments arranged by size: 1915 1396 1183 1177 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2190 2556 3094 4143 4170 5560 5747 Size: 110 380 366 538 1049 27 1390 187 Cuts at: 5747 1700 Size: 2869 Fragments arranged by size: 2869 1390 1049 538 380 366 187 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6916 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6916 BssSI C'ACGA_G Cuts at: 2075 4976 5988 2075 Size: 2901 1012 3003 Fragments arranged by size: 3003 2901 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2303 794 Size: 1509 5407 BstBI TT'CG_AA Cuts at: 2007 5720 2007 Size: 3713 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6916 BstXI CCAn_nnnn'nTGG Cuts at: 2954 3025 4096 2954 Size: 71 1071 5774 Fragments arranged by size: 5774 1071 71 BstYI r'GATC_y Cuts at: 1972 2115 4246 4613 4998 5015 6467 6553 6565 Size: 143 2131 367 385 17 1452 86 12 Cuts at: 6565 6911 1972 Size: 346 1977 Fragments arranged by size: 2131 1977 1452 385 367 346 143 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2868 3269 2868 Size: 401 6515 BtgI C'CryG_G Cuts at: 513 1065 2514 2599 3840 513 Size: 552 1449 85 1241 3589 Fragments arranged by size: 3589 1449 1241 552 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6177 Fragments arranged by size: 6177 683 56 BtsI GCAGTG_nn' Cuts at: 2511 2673 2724 3459 3529 3539 3923 4432 5226 Size: 162 51 735 70 10 384 509 794 Cuts at: 5226 5246 2511 Size: 20 4181 Fragments arranged by size: 4181 794 735 509 384 162 70 51 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6916 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3061 3096 529 Size: 35 2497 35 4349 Fragments arranged by size: 4349 2497 35 35 DraI TTT'AAA Cuts at: 4220 4220 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2845 5923 809 Size: 507 1529 3078 1802 Fragments arranged by size: 3078 1802 1529 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 4086 4141 Size: 54 1425 50 275 18 603 1653 55 Cuts at: 4141 4363 5252 6881 8 Size: 222 889 1629 43 Fragments arranged by size: 1653 1629 1425 889 603 275 222 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4141 4363 1537 Size: 275 2329 222 4090 Fragments arranged by size: 4090 2329 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2069 2178 3983 4846 1353 Size: 716 109 1805 863 3423 Fragments arranged by size: 3423 1805 863 716 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3176 4189 5503 5877 6023 387 Size: 1064 138 1587 1013 1314 374 146 1280 Fragments arranged by size: 1587 1314 1280 1064 1013 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2066 2111 2600 2786 3021 3565 Size: 393 428 6 45 489 186 235 544 Cuts at: 3565 3950 4979 5576 6363 6631 1239 Size: 385 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 787 597 544 489 428 393 385 268 235 186 45 6 EcoNI CCTnn'n_nnAGG Cuts at: 2550 3716 3974 2550 Size: 1166 258 5492 Fragments arranged by size: 5492 1166 258 EcoO109I rG'GnC_Cy Cuts at: 2143 2144 2348 2604 2922 2923 3394 3683 4023 Size: 1 204 256 318 1 471 289 340 Cuts at: 4023 2143 Size: 5036 Fragments arranged by size: 5036 471 340 318 289 256 204 1 1 EcoRI G'AATT_C Cuts at: 4225 4603 4225 Size: 378 6538 FspI TGC'GCA Cuts at: 2983 5422 2983 Size: 2439 4477 FspAI rTGC'GCAy Cuts at: 2983 2983 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 2191 3746 4126 4207 6063 1678 Size: 513 1555 380 81 1856 2531 Fragments arranged by size: 2531 1856 1555 513 380 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2177 2209 2213 Size: 32 135 32 550 32 23 32 4 Cuts at: 2213 2245 2864 2896 3168 3200 3494 3526 5557 Size: 32 619 32 272 32 294 32 2031 Cuts at: 5557 5589 5631 5663 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2031 619 550 294 272 135 42 32 32 32 32 32 32 32 32 32 32 23 4 HincII GTy'rAC Cuts at: 669 1657 1938 3646 4260 4506 669 Size: 988 281 1708 614 246 3079 Fragments arranged by size: 3079 1708 988 614 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6916 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KasI G'GCGC_C Cuts at: 4122 4122 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4515 4515 Size: 6916 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6030 6214 1305 Size: 4725 184 2007 Fragments arranged by size: 4725 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 4088 10 Size: 54 1425 343 603 1653 2838 Fragments arranged by size: 2838 1653 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2952 3117 4112 4874 5233 5392 518 Size: 648 1786 165 995 762 359 159 2042 Fragments arranged by size: 2042 1786 995 762 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2278 2806 2863 2902 3088 3429 4159 4332 Size: 672 528 57 39 186 341 730 173 Cuts at: 4332 5009 6157 6402 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 730 677 672 528 341 245 186 173 57 39 NaeI GCC'GGC Cuts at: 2558 3096 4145 2558 Size: 538 1049 5329 Fragments arranged by size: 5329 1049 538 NarI GG'CG_CC Cuts at: 4123 4123 Size: 6916 NcoI C'CATG_G Cuts at: 513 1065 2514 2599 513 Size: 552 1449 85 4830 Fragments arranged by size: 4830 1449 552 85 NdeI CA'TA_TG Cuts at: 387 387 Size: 6916 NgoMIV G'CCGG_C Cuts at: 2556 3094 4143 2556 Size: 538 1049 5329 Fragments arranged by size: 5329 1049 538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2263 2762 4084 4274 5819 1132 Size: 97 1034 499 1322 190 1545 2229 Fragments arranged by size: 2229 1545 1322 1034 499 190 97 PasI CC'CwG_GG Cuts at: 1348 2607 1348 Size: 1259 5657 PciI A'CATG_T Cuts at: 2259 5815 2259 Size: 3556 3360 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6916 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3245 3277 4985 5017 6498 6530 3245 Size: 32 1708 32 1481 32 3631 Fragments arranged by size: 3631 1708 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2348 3394 3683 4023 2348 Size: 1046 289 340 5241 Fragments arranged by size: 5241 1046 340 289 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PspOMI G'GGCC_C Cuts at: 2143 2603 2922 2143 Size: 460 319 6137 Fragments arranged by size: 6137 460 319 PstI C_TGCA'G Cuts at: 830 2333 2680 2802 2932 3345 4268 830 Size: 1503 347 122 130 413 923 3478 Fragments arranged by size: 3478 1503 923 413 347 130 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 2278 2806 2863 2278 Size: 528 57 6331 Fragments arranged by size: 6331 528 57 SalI G'TCGA_C Cuts at: 1936 4258 1936 Size: 2322 4594 SanDI GG'GwC_CC Cuts at: 2348 2348 Size: 6916 SapI GCTCTTCn'nnn_ Cuts at: 2178 2178 Size: 6916 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 2787 3203 5164 6876 2787 Size: 416 1961 1712 2827 Fragments arranged by size: 2827 1961 1712 416 SfcI C'TryA_G Cuts at: 826 1045 2329 2676 2798 2928 3341 4264 5399 Size: 219 1284 347 122 130 413 923 1135 Cuts at: 5399 6080 6271 826 Size: 681 191 1471 Fragments arranged by size: 1471 1284 1135 923 681 413 347 219 191 130 122 SfoI GGC'GCC Cuts at: 4124 4124 Size: 6916 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 4243 1872 Size: 2371 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2850 3886 3971 5020 5921 6183 Size: 197 926 907 1036 85 1049 901 262 Cuts at: 6183 820 Size: 1553 Fragments arranged by size: 1553 1049 1036 926 907 901 262 197 85 SnaBI TAC'GTA Cuts at: 493 4213 4707 493 Size: 3720 494 2702 Fragments arranged by size: 3720 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 4084 4274 1132 Size: 2952 190 3774 Fragments arranged by size: 3774 2952 190 SspI AAT'ATT Cuts at: 5 52 4840 5 Size: 47 4788 2081 Fragments arranged by size: 4788 2081 47 StuI AGG'CCT Cuts at: 1517 2061 3702 3874 1517 Size: 544 1641 172 4559 Fragments arranged by size: 4559 1641 544 172 StyI C'CwwG_G Cuts at: 513 1065 2214 2379 2436 2514 2599 3606 3790 Size: 552 1149 165 57 78 85 1007 184 Cuts at: 3790 3909 4126 4355 513 Size: 119 217 229 3074 Fragments arranged by size: 3074 1149 1007 552 229 217 184 165 119 85 78 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3493 3922 4943 4960 5113 5298 5743 Size: 1255 1913 429 1021 17 153 185 445 Cuts at: 5743 325 Size: 1498 Fragments arranged by size: 1913 1498 1255 1021 445 429 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2785 3201 3497 Size: 274 80 33 51 1443 808 416 296 Cuts at: 3497 5162 6874 96 Size: 1665 1712 138 Fragments arranged by size: 1712 1665 1443 808 416 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2609 2641 6876 6908 2460 Size: 32 117 32 4235 32 2468 Fragments arranged by size: 4235 2468 117 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6916 XbaI T'CTAG_A Cuts at: 3103 4252 3103 Size: 1149 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3361 3814 3916 3361 Size: 453 102 6361 Fragments arranged by size: 6361 453 102 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6916 XmaI C'CCGG_G Cuts at: 1870 4241 1870 Size: 2371 4545 XmnI GAAnn'nnTTC Cuts at: 2372 3998 5045 2372 Size: 1626 1047 4243 Fragments arranged by size: 4243 1626 1047 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4724 276 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI