Restriction map for
pFN21ASDA0251
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BbeI BclI BglI BglII BlnI
BsaHI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluNI MscI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StyI SwaI
XbaI XcmI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2032 GTCTAC GT^[AC][GT]AC
AccIII 130 TCCGGA T^CCGGA
AcsI 380 GAATTT [GA]^AATT[CT]
AflII 2358 CTTAAG C^TTAAG
ApoI 380 GAATTT [GA]^AATT[CT]
BamHI 631 GGATCC G^GATCC
BanI 1317 GGTGCC G^G[CT][GA]CC
BbrPI 1362 CACGTG CAC^GTG
BcgI 948 CGATGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 347 GGATAC GTATCC(6/5)
BfrI 2358 CTTAAG C^TTAAG
BsaAI 1362 CACGTG [CT]AC^GT[GA]
BsaBI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 130 TCCGGA T^CCGGA
BsgI 2048 GTGCAG GTGCAG(16/14)
BsiEI 1254 CGGCCG CG[GA][CT]^CG
BsmI 1847 GCATTC GAATGC(1/-1)
BspEI 130 TCCGGA T^CCGGA
BspMI 1519 GCAGGT ACCTGC(4/8)
BsrDI 1773 CATTGC GCAATG(2/0)
DraIII 2042 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 901 CCGTGG C^C[GA][CT]GG
EagI 1254 CGGCCG C^GGCCG
EclXI 1254 CGGCCG C^GGCCG
Eco52I 1254 CGGCCG C^GGCCG
EcoT22I 205 ATGCAT ATGCA^T
HaeII 2198 AGCGCC [GA]GCGC^[CT]
MamI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5 ACGCGT A^CGCGT
MroI 130 TCCGGA T^CCGGA
NsiI 205 ATGCAT ATGCA^T
PflMI 362 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1362 CACGTG CAC^GTG
PmlI 1362 CACGTG CAC^GTG
SapI 1710 GAAGAGC GCTCTTC(1/4)
SmaI 1787 CCCGGG CCC^GGG
SphI 1074 GCATGC GCATG^C
SspI 250 AATATT AAT^ATT
StuI 2294 AGGCCT AGG^CCT
Van91I 362 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1787 CCCGGG C^CCGGG
XmaI 1787 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 6 CGCG CG^CG
1278 CGCG
AfaI 610 GTAC GT^AC
1472 GTAC
AflIII 5 ACGCGT A^C[GA][CT]GT
1363 ACGTGT
Asp700I 1831 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1888 GAACGGCTTC
AspHI 1150 GAGCTC G[AT]GC[AT]^C
1759 GAGCTC
AspI 284 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAACGTC
BbvCI 2272 CCTCAGC CCTCAGC(-5/-2)
1354 GCTGAGG
BfaI 344 CTAG C^TAG
545 CTAG
BlpI 1152 GCTCAGC GC^T[ACGT]AGC
1756 GCTGAGC
Bpu1102I 1152 GCTCAGC GC^T[ACGT]AGC
1756 GCTGAGC
BsiHKAI 1150 GAGCTC G[AT]GC[AT]^C
1759 GAGCTC
BssSI 1269 CTCGTG CACGAG(-5/-1)
1762 CTCGTG
BstAPI 1311 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2041 GCACAAGGTGC
BstUI 6 CGCG CG^CG
1278 CGCG
BstXI 153 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
246 CCAAAATATTGG
CelII 1152 GCTCAGC GC^T[ACGT]AGC
1756 GCTGAGC
CfoI 1279 GCGC GCG^C
2199 GCGC
EaeI 1254 CGGCCG [CT]^GGCC[GA]
1778 CGGCCA
HhaI 1279 GCGC GCG^C
2199 GCGC
HinP1I 1279 GCGC G^CGC
2199 GCGC
HincII 1082 GTCAAC GT[CT]^[GA]AC
1091 GTCAAC
HindII 1082 GTCAAC GT[CT]^[GA]AC
1091 GTCAAC
MaeI 344 CTAG C^TAG
545 CTAG
MvnI 6 CGCG CG^CG
1278 CGCG
NdeI 268 CATATG CA^TATG
792 CATATG
NspI 1074 GCATGC [GA]CATG^[CT]
1578 ACATGC
PaeR7I 1806 CTCGAG C^TCGAG
2230 CTCGAG
PflFI 284 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAACGTC
PpuMI 2125 AGGTCCC [GA]G^G[AT]CC[CT]
2309 GGGTCCC
PstI 863 CTGCAG CTGCA^G
1870 CTGCAG
RsaI 610 GTAC GT^AC
1472 GTAC
SacI 1150 GAGCTC GAGCT^C
1759 GAGCTC
Tth111I 284 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1741 GACAACGTC
XhoI 1806 CTCGAG C^TCGAG
2230 CTCGAG
XmnI 1831 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1888 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII