(Circular) (Six-base) MAPSORT of: pFN21ASDA0251.seq Check: 3355 from: 1 to: 7093 pFN21ASDA0251 7093 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4903 278 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4056 4436 1937 Size: 2119 380 4594 Fragments arranged by size: 4594 2119 380 Acc65I G'GTAC_C Cuts at: 4414 4414 Size: 7093 AclI AA'CG_TT Cuts at: 1563 5220 5593 1563 Size: 3657 373 3063 Fragments arranged by size: 3657 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3252 3350 3689 5156 6540 6808 1632 Size: 1620 98 339 1467 1384 268 1917 Fragments arranged by size: 1917 1620 1467 1384 339 268 98 AflII C'TTAA_G Cuts at: 820 1017 4381 820 Size: 197 3364 3532 Fragments arranged by size: 3532 3364 197 AflIII A'CryG_T Cuts at: 1203 2028 3386 5992 6969 1203 Size: 825 1358 2606 977 1327 Fragments arranged by size: 2606 1358 1327 977 825 AgeI A'CCGG_T Cuts at: 5924 5924 Size: 7093 AhdI GACnn_n'nnGTC Cuts at: 5822 5822 Size: 7093 AleI CACnn'nnGTG Cuts at: 1166 3294 1166 Size: 2128 4965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3407 1184 Size: 2223 4870 AlwNI CAG_nnn'CTG Cuts at: 4836 6408 4836 Size: 1572 5521 ApaLI G'TGCA_C Cuts at: 5150 6306 5150 Size: 1156 5937 ApoI r'AATT_y Cuts at: 1493 2403 4402 4628 4780 6889 1493 Size: 910 1999 226 152 2109 1697 Fragments arranged by size: 2109 1999 1697 910 226 152 AseI AT'TA_AT Cuts at: 160 1032 5647 160 Size: 872 4615 1606 Fragments arranged by size: 4615 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 AvaI C'yCGr_G Cuts at: 1870 1943 3432 3810 3829 4099 4253 4418 1870 Size: 73 1489 378 19 270 154 165 4545 Fragments arranged by size: 4545 1489 378 270 165 154 73 19 BamHI G'GATC_C Cuts at: 2654 4423 4790 2654 Size: 1769 367 4957 Fragments arranged by size: 4957 1769 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3340 4414 5869 618 Size: 325 217 627 1553 1074 1455 1842 Fragments arranged by size: 1842 1553 1455 1074 627 325 217 BanII G_rGCy'C Cuts at: 1391 2253 2261 3177 3786 3959 4288 4412 1391 Size: 862 8 916 609 173 329 124 4072 Fragments arranged by size: 4072 916 862 609 329 173 124 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 2725 4209 928 Size: 971 606 220 1484 3812 Fragments arranged by size: 3812 1484 971 606 220 BbvCI CC'TCA_GC Cuts at: 3378 4296 3378 Size: 918 6175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2960 2994 5284 5318 1903 Size: 34 1023 34 2290 34 3678 Fragments arranged by size: 3678 2290 1023 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2364 4985 6195 2364 Size: 2621 1210 3262 Fragments arranged by size: 3262 2621 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7093 BfrBI ATG'CAT Cuts at: 2230 2230 Size: 7093 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5704 136 Size: 107 122 71 631 605 4032 1525 Fragments arranged by size: 4032 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7088 7088 Size: 7093 BlpI GC'TnA_GC Cuts at: 3176 3780 4510 3176 Size: 604 730 5759 Fragments arranged by size: 5759 730 604 Bme1580I G_kGCm'C Cuts at: 1790 3037 3255 3386 5154 6310 1790 Size: 1247 218 131 1768 1156 2573 Fragments arranged by size: 2573 1768 1247 1156 218 131 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3179 3242 4824 Size: 416 53 83 399 24 1762 63 1582 Cuts at: 4824 5782 442 Size: 958 1753 Fragments arranged by size: 1762 1753 1582 958 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7093 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3196 3307 3844 4201 3196 Size: 111 537 357 6088 Fragments arranged by size: 6088 537 357 111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2123 2159 2192 2705 3485 4052 5753 Size: 818 66 36 33 513 780 567 1701 Cuts at: 5753 1239 Size: 2579 Fragments arranged by size: 2579 1701 818 780 567 513 66 36 33 Bpu10I CC'TnA_GC Cuts at: 3378 4296 3378 Size: 918 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3867 5218 6083 6381 3867 Size: 1351 865 298 4579 Fragments arranged by size: 4579 1351 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3551 3785 4172 5756 882 Size: 617 2052 234 387 1584 2219 Fragments arranged by size: 2219 2052 1584 617 387 234 BsaAI yAC'GTr Cuts at: 493 1204 3387 4390 4884 493 Size: 711 2183 1003 494 2702 Fragments arranged by size: 2702 2183 1003 711 494 BsaBI GATnn'nnATC Cuts at: 3447 7087 3447 Size: 3640 3453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4900 5282 5917 275 Size: 53 83 186 936 3367 382 635 1451 Fragments arranged by size: 3367 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2153 3229 3979 4372 5526 5924 6198 6345 6756 Size: 1076 750 393 1154 398 274 147 411 Cuts at: 6756 2153 Size: 2490 Fragments arranged by size: 2490 1154 1076 750 411 398 393 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2899 2929 3429 3459 4072 Size: 30 165 30 1496 30 500 30 613 Cuts at: 4072 4102 1178 Size: 30 4169 Fragments arranged by size: 4169 1496 613 500 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2860 3836 1186 Size: 1075 599 976 4443 Fragments arranged by size: 4443 1075 976 599 BseYI C'CCAG_C Cuts at: 2658 2929 3316 4289 6296 6792 2658 Size: 271 387 973 2007 496 2959 Fragments arranged by size: 2959 2007 973 496 387 271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4092 1163 Size: 573 2356 4164 Fragments arranged by size: 4164 2356 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4543 5304 6332 1540 Size: 275 186 1279 1263 761 1028 2301 Fragments arranged by size: 2301 1279 1263 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3177 3786 5154 5239 6310 3177 Size: 609 1368 85 1071 3960 Fragments arranged by size: 3960 1368 1071 609 85 BsmI GAATG_Cn' Cuts at: 3870 4604 4697 3870 Size: 734 93 6266 Fragments arranged by size: 6266 734 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2261 3037 3177 3255 3386 3786 Size: 399 463 8 776 140 78 131 400 Cuts at: 3786 3959 4288 4412 5154 5239 6310 1391 Size: 173 329 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 776 742 463 400 399 329 173 140 131 124 85 78 8 BspEI T'CCGG_A Cuts at: 2153 6756 2153 Size: 4603 2490 BspHI T'CATG_A Cuts at: 4982 6712 4982 Size: 1730 5363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3533 4448 844 Size: 2689 915 3489 Fragments arranged by size: 3489 2689 915 BsrBI CCG'CTC Cuts at: 1394 1823 4980 1394 Size: 429 3157 3507 Fragments arranged by size: 3507 3157 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3795 5588 5762 65 Size: 1183 2547 1793 174 1396 Fragments arranged by size: 2547 1793 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5737 5924 1700 Size: 110 3927 187 2869 Fragments arranged by size: 3927 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7093 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7093 BssSI C'ACGA_G Cuts at: 3292 3785 5153 6165 3292 Size: 493 1368 1012 4220 Fragments arranged by size: 4220 1368 1012 493 BstAPI GCAn_nnn'nTGC Cuts at: 794 3340 4070 794 Size: 2546 730 3817 Fragments arranged by size: 3817 2546 730 BstBI TT'CG_AA Cuts at: 2007 5897 2007 Size: 3890 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7093 BstXI CCAn_nnnn'nTGG Cuts at: 2183 2276 2183 Size: 93 7000 BstYI r'GATC_y Cuts at: 1972 2654 3226 3367 3634 3942 4423 4790 5175 Size: 682 572 141 267 308 481 367 385 Cuts at: 5175 5192 6644 6730 6742 7088 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 682 572 481 385 367 346 308 267 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2924 513 Size: 552 957 902 4682 Fragments arranged by size: 4682 957 902 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6354 Fragments arranged by size: 6354 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2188 4609 5403 5423 2097 Size: 91 2421 794 20 3767 Fragments arranged by size: 3767 2421 794 91 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7093 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7058 DraI TTT'AAA Cuts at: 3238 3706 4144 4397 3238 Size: 468 438 253 5934 Fragments arranged by size: 5934 468 438 253 DraIII CAC_nnn'GTG Cuts at: 4070 4070 Size: 7093 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6100 809 Size: 507 4784 1802 Fragments arranged by size: 4784 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 3801 4540 Size: 54 1425 50 275 18 1447 524 739 Cuts at: 4540 5429 7058 8 Size: 889 1629 43 Fragments arranged by size: 1629 1447 1425 889 739 524 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 4540 1537 Size: 275 1465 1263 4090 Fragments arranged by size: 4090 1465 1263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2436 3507 3728 3918 5023 1353 Size: 768 315 1071 221 190 1105 3423 Fragments arranged by size: 3423 1105 1071 768 315 221 190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5680 6054 6200 387 Size: 1064 138 4091 374 146 1280 Fragments arranged by size: 4091 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3175 3784 3175 Size: 609 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2123 2159 2192 2705 3252 3350 Size: 393 425 66 36 33 513 547 98 Cuts at: 3350 3485 3689 4052 5156 5753 6540 6808 1239 Size: 135 204 363 1104 597 787 268 1524 Fragments arranged by size: 1524 1104 787 597 547 513 425 393 363 268 204 135 98 66 36 33 EcoO109I rG'GnC_Cy Cuts at: 3713 4149 4333 3713 Size: 436 184 6473 Fragments arranged by size: 6473 436 184 EcoRI G'AATT_C Cuts at: 4402 4780 4402 Size: 378 6715 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3932 3932 Size: 7093 FspI TGC'GCA Cuts at: 5599 5599 Size: 7093 HaeII r_GCGC'y Cuts at: 1678 4225 6240 1678 Size: 2547 2015 2531 Fragments arranged by size: 2547 2531 2015 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2899 2931 4211 4243 4340 Size: 32 135 32 1327 32 1280 32 97 Cuts at: 4340 4372 5734 5766 5808 5840 1373 Size: 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 1327 1280 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3107 3116 4437 4683 669 Size: 988 281 1169 9 1321 246 3079 Fragments arranged by size: 3079 1321 1169 988 281 246 9 HindIII A'AGCT_T Cuts at: 748 748 Size: 7093 HpaI GTT'AAC Cuts at: 4683 4683 Size: 7093 KpnI G_GTAC'C Cuts at: 4418 4418 Size: 7093 MfeI C'AATT_G Cuts at: 4692 4692 Size: 7093 MluI A'CGCG_T Cuts at: 2028 6969 2028 Size: 4941 2152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4115 6207 6391 1305 Size: 2810 2092 184 2007 Fragments arranged by size: 2810 2092 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5271 Fragments arranged by size: 5271 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2235 2909 3294 3362 3606 5051 5410 Size: 648 1069 674 385 68 244 1445 359 Cuts at: 5410 5569 518 Size: 159 2042 Fragments arranged by size: 2042 1445 1069 674 648 385 359 244 159 68 MspA1I CmG'CkG Cuts at: 1606 2942 3328 3337 4509 5186 6334 6579 1606 Size: 1336 386 9 1172 677 1148 245 2120 Fragments arranged by size: 2120 1336 1172 1148 677 386 245 9 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5584 Fragments arranged by size: 5584 957 552 NdeI CA'TA_TG Cuts at: 387 2292 2816 387 Size: 1905 524 4664 Fragments arranged by size: 4664 1905 524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7093 NotI GC'GGCC_GC Cuts at: 4540 4540 Size: 7093 NsiI A_TGCA'T Cuts at: 2232 2232 Size: 7093 NspI r_CATG'y Cuts at: 1132 1229 3101 3605 4451 5996 1132 Size: 97 1872 504 846 1545 2229 Fragments arranged by size: 2229 1872 1545 846 504 97 PasI CC'CwG_GG Cuts at: 1348 4280 1348 Size: 2932 4161 PciI A'CATG_T Cuts at: 5992 5992 Size: 7093 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 1108 Size: 1283 5810 PfoI T'CCnGG_A Cuts at: 2682 3207 2682 Size: 525 6568 PmeI GTTT'AAAC Cuts at: 4397 4397 Size: 7093 PmlI CAC'GTG Cuts at: 3387 3387 Size: 7093 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5162 5194 6675 6707 5162 Size: 32 1481 32 5548 Fragments arranged by size: 5548 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4149 4333 4149 Size: 184 6909 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7093 PsiI TTA'TAA Cuts at: 4663 4663 Size: 7093 PspXI vC'TCGA_Gb Cuts at: 3829 4253 3829 Size: 424 6669 PstI C_TGCA'G Cuts at: 830 2890 3897 4445 830 Size: 2060 1007 548 3478 Fragments arranged by size: 3478 2060 1007 548 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7093 PvuII CAG'CTG Cuts at: 2942 3328 3337 2942 Size: 386 9 6698 Fragments arranged by size: 6698 386 9 SacI G_AGCT'C Cuts at: 3177 3786 3177 Size: 609 6484 SalI G'TCGA_C Cuts at: 1936 4435 1936 Size: 2499 4594 SanDI GG'GwC_CC Cuts at: 4333 4333 Size: 7093 SapI GCTCTTCn'nnn_ Cuts at: 3728 3728 Size: 7093 SbfI CC_TGCA'GG Cuts at: 4445 4445 Size: 7093 ScaI AGT'ACT Cuts at: 5341 7053 5341 Size: 1712 5381 SfcI C'TryA_G Cuts at: 826 1045 2886 3893 3948 4441 5576 6257 6448 Size: 219 1841 1007 55 493 1135 681 191 Cuts at: 6448 826 Size: 1471 Fragments arranged by size: 1841 1471 1135 1007 681 493 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7093 SmaI CCC'GGG Cuts at: 1872 3812 4420 1872 Size: 1940 608 4545 Fragments arranged by size: 4545 1940 608 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 3882 4253 4381 5197 6098 Size: 197 926 1886 53 371 128 816 901 Cuts at: 6098 6360 820 Size: 262 1553 Fragments arranged by size: 1886 1553 926 901 816 371 262 197 128 53 SnaBI TAC'GTA Cuts at: 493 4390 4884 493 Size: 3897 494 2702 Fragments arranged by size: 3897 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7093 SphI G_CATG'C Cuts at: 1132 3101 4451 1132 Size: 1969 1350 3774 Fragments arranged by size: 3774 1969 1350 SspI AAT'ATT Cuts at: 5 52 2275 5017 5 Size: 47 2223 2742 2081 Fragments arranged by size: 2742 2223 2081 47 StuI AGG'CCT Cuts at: 1517 4319 1517 Size: 2802 4291 StyI C'CwwG_G Cuts at: 513 1065 2022 4532 513 Size: 552 957 2510 3074 Fragments arranged by size: 3074 2510 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2412 4293 4328 4356 5120 5137 5290 Size: 1255 832 1881 35 28 764 17 153 Cuts at: 5290 5475 5920 325 Size: 185 445 1498 Fragments arranged by size: 1881 1498 1255 832 764 445 185 153 35 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5339 7051 96 Size: 274 80 33 51 1443 3362 1712 138 Fragments arranged by size: 3362 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3429 3461 4072 4104 7053 7085 3429 Size: 32 611 32 2949 32 3437 Fragments arranged by size: 3437 2949 611 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2310 3767 1316 Size: 994 1457 4642 Fragments arranged by size: 4642 1457 994 XbaI T'CTAG_A Cuts at: 4429 4429 Size: 7093 XhoI C'TCGA_G Cuts at: 1943 3829 4253 1943 Size: 1886 424 4783 Fragments arranged by size: 4783 1886 424 XmaI C'CCGG_G Cuts at: 1870 3810 4418 1870 Size: 1940 608 4545 Fragments arranged by size: 4545 1940 608 XmnI GAAnn'nnTTC Cuts at: 3858 3915 5222 3858 Size: 57 1307 5729 Fragments arranged by size: 5729 1307 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4901 276 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI