(Circular) (Six-base) MAPSORT of: pFN21ASDA0250.seq Check: 1787 from: 1 to: 8002 pFN21ASDA0250 8002 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5812 278 Size: 53 83 186 936 4276 2468 Fragments arranged by size: 4276 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4148 5345 1937 Size: 2211 1197 4594 Fragments arranged by size: 4594 2211 1197 Acc65I G'GTAC_C Cuts at: 5323 5323 Size: 8002 AclI AA'CG_TT Cuts at: 1563 6129 6502 1563 Size: 4566 373 3063 Fragments arranged by size: 4566 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2124 3786 3956 4334 6065 7449 7717 Size: 455 37 1662 170 378 1731 1384 268 Cuts at: 7717 1632 Size: 1917 Fragments arranged by size: 1917 1731 1662 1384 455 378 268 170 37 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7805 AflIII A'CryG_T Cuts at: 1203 6901 7878 1203 Size: 5698 977 1327 Fragments arranged by size: 5698 1327 977 AgeI A'CCGG_T Cuts at: 6833 6833 Size: 8002 AhdI GACnn_n'nnGTC Cuts at: 3149 6731 3149 Size: 3582 4420 AleI CACnn'nnGTG Cuts at: 1166 2946 1166 Size: 1780 6222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8002 AlwNI CAG_nnn'CTG Cuts at: 2128 2137 3016 3764 5745 7317 2128 Size: 9 879 748 1981 1572 2813 Fragments arranged by size: 2813 1981 1572 879 748 9 ApaLI G'TGCA_C Cuts at: 6059 7215 6059 Size: 1156 6846 ApoI r'AATT_y Cuts at: 1493 2786 3616 4487 5311 5537 5689 7798 1493 Size: 1293 830 871 824 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 871 830 824 226 152 AseI AT'TA_AT Cuts at: 160 1032 3497 6556 160 Size: 872 2465 3059 1606 Fragments arranged by size: 3059 2465 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8002 AvaI C'yCGr_G Cuts at: 1870 1943 5327 1870 Size: 73 3384 4545 Fragments arranged by size: 4545 3384 73 BamHI G'GATC_C Cuts at: 4600 5332 5699 4600 Size: 732 367 6903 Fragments arranged by size: 6903 732 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5120 5276 5323 6778 618 Size: 325 217 627 3333 156 47 1455 1842 Fragments arranged by size: 3333 1842 1455 627 325 217 156 47 BanII G_rGCy'C Cuts at: 1391 2834 3584 3926 4656 5321 1391 Size: 1443 750 342 730 665 4072 Fragments arranged by size: 4072 1443 750 730 665 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2382 3513 928 Size: 971 483 1131 5417 Fragments arranged by size: 5417 1131 971 483 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6193 6227 1903 Size: 34 4256 34 3678 Fragments arranged by size: 4256 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5894 7104 5894 Size: 1210 6792 BclI T'GATC_A Cuts at: 1548 1548 Size: 8002 BfrBI ATG'CAT Cuts at: 5209 5209 Size: 8002 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6613 136 Size: 107 122 71 631 605 4941 1525 Fragments arranged by size: 4941 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4526 7997 4526 Size: 3471 4531 BlpI GC'TnA_GC Cuts at: 2559 4131 5419 2559 Size: 1572 1288 5142 Fragments arranged by size: 5142 1572 1288 Bme1580I G_kGCm'C Cuts at: 1790 6063 7219 1790 Size: 4273 1156 2573 Fragments arranged by size: 4273 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8002 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 3811 4504 Size: 416 53 83 399 24 2090 304 693 Cuts at: 4504 5733 6691 442 Size: 1229 958 1753 Fragments arranged by size: 2090 1753 1229 958 693 416 399 304 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8002 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3259 4663 5173 3259 Size: 1404 510 6088 Fragments arranged by size: 6088 1404 510 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3741 3861 3975 4971 5249 6662 1239 Size: 2502 120 114 996 278 1413 2579 Fragments arranged by size: 2579 2502 1413 996 278 120 114 Bpu10I CC'TnA_GC Cuts at: 3925 3925 Size: 8002 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3229 3786 6127 6992 7290 3229 Size: 557 2341 865 298 3941 Fragments arranged by size: 3941 2341 865 557 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2616 3124 3298 3690 4848 6665 882 Size: 617 1117 508 174 392 1158 1817 2219 Fragments arranged by size: 2219 1817 1158 1117 617 508 392 174 BsaAI yAC'GTr Cuts at: 493 1204 5299 5793 493 Size: 711 4095 494 2702 Fragments arranged by size: 4095 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7996 7996 Size: 8002 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5809 6191 6826 275 Size: 53 83 186 936 4276 382 635 1451 Fragments arranged by size: 4276 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4688 6435 6833 7107 7254 7665 4688 Size: 1747 398 274 147 411 5025 Fragments arranged by size: 5025 1747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5222 5252 1178 Size: 30 165 30 3819 30 3928 Fragments arranged by size: 3928 3819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3095 3287 3843 4884 4920 1186 Size: 1909 192 556 1041 36 4268 Fragments arranged by size: 4268 1909 1041 556 192 36 BseYI C'CCAG_C Cuts at: 2092 2680 3860 3940 4013 4104 4922 7205 7701 Size: 588 1180 80 73 91 818 2283 496 Cuts at: 7701 2092 Size: 2393 Fragments arranged by size: 2393 2283 1180 818 588 496 91 80 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5452 6213 7241 1540 Size: 275 186 3451 761 1028 2301 Fragments arranged by size: 3451 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4656 6063 6148 7219 4656 Size: 1407 85 1071 5439 Fragments arranged by size: 5439 1407 1071 85 BsmI GAATG_Cn' Cuts at: 4620 5513 5606 4620 Size: 893 93 7016 Fragments arranged by size: 7016 893 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2834 3584 3926 4656 5321 6063 6148 Size: 399 1044 750 342 730 665 742 85 Cuts at: 6148 7219 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1044 750 742 730 665 399 342 85 BspEI T'CCGG_A Cuts at: 7665 7665 Size: 8002 BspHI T'CATG_A Cuts at: 5891 7621 5891 Size: 1730 6272 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4153 5357 844 Size: 3309 1204 3489 Fragments arranged by size: 3489 3309 1204 BsrBI CCG'CTC Cuts at: 1394 1823 5889 1394 Size: 429 4066 3507 Fragments arranged by size: 4066 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 2658 2965 4385 6497 6671 65 Size: 1183 1254 156 307 1420 2112 174 1396 Fragments arranged by size: 2112 1420 1396 1254 1183 307 174 156 BsrFI r'CCGG_y Cuts at: 1700 1810 4210 6646 6833 1700 Size: 110 2400 2436 187 2869 Fragments arranged by size: 2869 2436 2400 187 110 BsrGI T'GTAC_A Cuts at: 96 2351 96 Size: 2255 5747 BssHII G'CGCG_C Cuts at: 1925 2037 1925 Size: 112 7890 BssSI C'ACGA_G Cuts at: 3681 6062 7074 3681 Size: 2381 1012 4609 Fragments arranged by size: 4609 2381 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2128 3048 794 Size: 1334 920 5748 Fragments arranged by size: 5748 1334 920 BstBI TT'CG_AA Cuts at: 2007 6806 2007 Size: 4799 3203 BstEII G'GTnAC_C Cuts at: 1184 3338 3655 4572 1184 Size: 2154 317 917 4614 Fragments arranged by size: 4614 2154 917 317 BstYI r'GATC_y Cuts at: 1972 2197 4526 4600 5332 5699 6084 6101 7553 Size: 225 2329 74 732 367 385 17 1452 Cuts at: 7553 7639 7651 7997 1972 Size: 86 12 346 1977 Fragments arranged by size: 2329 1977 1452 732 385 367 346 225 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 4296 4296 Size: 8002 BtgI C'CryG_G Cuts at: 513 1065 2791 3123 4443 4838 5040 5134 5145 Size: 552 1726 332 1320 395 202 94 11 Cuts at: 5145 513 Size: 3370 Fragments arranged by size: 3370 1726 1320 552 395 332 202 94 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7263 Fragments arranged by size: 7263 683 56 BtsI GCAGTG_nn' Cuts at: 2479 3171 5518 6312 6332 2479 Size: 692 2347 794 20 4149 Fragments arranged by size: 4149 2347 794 692 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8002 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7967 DraI TTT'AAA Cuts at: 5306 5306 Size: 8002 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3760 4840 7009 809 Size: 507 2444 1080 2169 1802 Fragments arranged by size: 2444 2169 1802 1080 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5449 6338 7967 Size: 54 1425 50 275 18 3619 889 1629 Cuts at: 7967 8 Size: 43 Fragments arranged by size: 3619 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5449 1537 Size: 275 3637 4090 Fragments arranged by size: 4090 3637 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2841 3108 3213 3228 3861 5932 1353 Size: 1488 267 105 15 633 2071 3423 Fragments arranged by size: 3423 2071 1488 633 267 105 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3867 6589 6963 7109 387 Size: 1064 138 2278 2722 374 146 1280 Fragments arranged by size: 2722 2278 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4654 4654 Size: 8002 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2124 3741 3786 3861 3956 3975 Size: 393 455 37 1617 45 75 95 19 Cuts at: 3975 4334 4971 5249 6065 6662 7449 7717 1239 Size: 359 637 278 816 597 787 268 1524 Fragments arranged by size: 1617 1524 816 787 637 597 455 393 359 278 268 95 75 45 37 19 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2545 2051 Size: 494 7508 EcoO109I rG'GnC_Cy Cuts at: 2134 3238 4079 4480 2134 Size: 1104 841 401 5656 Fragments arranged by size: 5656 1104 841 401 EcoRI G'AATT_C Cuts at: 4487 5311 5689 4487 Size: 824 378 6800 Fragments arranged by size: 6800 824 378 FspI TGC'GCA Cuts at: 6508 6508 Size: 8002 HaeII r_GCGC'y Cuts at: 1678 2436 7149 1678 Size: 758 4713 2531 Fragments arranged by size: 4713 2531 758 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3135 3167 3716 3748 4511 Size: 32 135 32 1563 32 549 32 763 Cuts at: 4511 4543 4643 4675 4683 4715 4999 5031 5051 Size: 32 100 32 8 32 284 32 20 Cuts at: 5051 5083 6643 6675 6717 6749 1373 Size: 32 1560 32 42 32 2626 Fragments arranged by size: 2626 1563 1560 763 549 284 135 100 42 32 32 32 32 32 32 32 32 32 32 32 20 8 HincII GTy'rAC Cuts at: 669 1657 1938 5346 5592 669 Size: 988 281 3408 246 3079 Fragments arranged by size: 3408 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2883 748 Size: 2135 5867 HpaI GTT'AAC Cuts at: 5592 5592 Size: 8002 KpnI G_GTAC'C Cuts at: 5327 5327 Size: 8002 MfeI C'AATT_G Cuts at: 2396 5601 2396 Size: 3205 4797 MluI A'CGCG_T Cuts at: 7878 7878 Size: 8002 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2984 4958 7116 7300 1305 Size: 1679 1974 2158 184 2007 Fragments arranged by size: 2158 2007 1974 1679 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6180 Fragments arranged by size: 6180 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 2796 2946 3228 5960 6319 6478 Size: 648 1497 133 150 282 2732 359 159 Cuts at: 6478 518 Size: 2042 Fragments arranged by size: 2732 2042 1497 648 359 282 159 150 133 MspA1I CmG'CkG Cuts at: 1606 2102 2684 3944 4159 4282 5236 5418 6095 Size: 496 582 1260 215 123 954 182 677 Cuts at: 6095 7243 7488 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1260 1148 954 677 582 496 245 215 182 123 NaeI GCC'GGC Cuts at: 4212 4212 Size: 8002 NcoI C'CATG_G Cuts at: 513 1065 3123 4443 5040 5134 5145 513 Size: 552 2058 1320 597 94 11 3370 Fragments arranged by size: 3370 2058 1320 597 552 94 11 NdeI CA'TA_TG Cuts at: 387 3889 387 Size: 3502 4500 NgoMIV G'CCGG_C Cuts at: 4210 4210 Size: 8002 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8002 NotI GC'GGCC_GC Cuts at: 5449 5449 Size: 8002 NsiI A_TGCA'T Cuts at: 5211 5211 Size: 8002 NspI r_CATG'y Cuts at: 1132 1229 2555 5360 6905 1132 Size: 97 1326 2805 1545 2229 Fragments arranged by size: 2805 2229 1545 1326 97 PasI CC'CwG_GG Cuts at: 1348 3147 3584 1348 Size: 1799 437 5766 Fragments arranged by size: 5766 1799 437 PciI A'CATG_T Cuts at: 6901 6901 Size: 8002 PflMI CCAn_nnn'nTGG Cuts at: 1108 3129 1108 Size: 2021 5981 PfoI T'CCnGG_A Cuts at: 5212 5212 Size: 8002 PmeI GTTT'AAAC Cuts at: 5306 5306 Size: 8002 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2184 2216 6071 6103 7584 7616 2184 Size: 32 3855 32 1481 32 2570 Fragments arranged by size: 3855 2570 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 3238 4079 4480 2134 Size: 1104 841 401 5656 Fragments arranged by size: 5656 1104 841 401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8002 PsiI TTA'TAA Cuts at: 2589 5572 2589 Size: 2983 5019 PstI C_TGCA'G Cuts at: 830 2034 2133 2654 3574 4164 5014 5354 830 Size: 1204 99 521 920 590 850 340 3478 Fragments arranged by size: 3478 1204 920 850 590 521 340 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8002 PvuII CAG'CTG Cuts at: 2102 2684 3944 4159 4282 5236 2102 Size: 582 1260 215 123 954 4868 Fragments arranged by size: 4868 1260 954 582 215 123 SacI G_AGCT'C Cuts at: 4656 4656 Size: 8002 SalI G'TCGA_C Cuts at: 1936 5344 1936 Size: 3408 4594 SbfI CC_TGCA'GG Cuts at: 5354 5354 Size: 8002 ScaI AGT'ACT Cuts at: 6250 7962 6250 Size: 1712 6290 SexAI A'CCwGG_T Cuts at: 4002 4002 Size: 8002 SfcI C'TryA_G Cuts at: 826 1045 2030 2129 2545 2650 3062 3405 3570 Size: 219 985 99 416 105 412 343 165 Cuts at: 3570 3978 4160 4507 5010 5350 6485 7166 7357 Size: 408 182 347 503 340 1135 681 191 Cuts at: 7357 826 Size: 1471 Fragments arranged by size: 1471 1135 985 681 503 416 412 408 347 343 340 219 191 182 165 105 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8002 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8002 SmaI CCC'GGG Cuts at: 1872 5329 1872 Size: 3457 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3244 3801 6106 7007 7269 820 Size: 197 926 1301 557 2305 901 262 1553 Fragments arranged by size: 2305 1553 1301 926 901 557 262 197 SnaBI TAC'GTA Cuts at: 493 5299 5793 493 Size: 4806 494 2702 Fragments arranged by size: 4806 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8002 SphI G_CATG'C Cuts at: 1132 2555 5360 1132 Size: 1423 2805 3774 Fragments arranged by size: 3774 2805 1423 SspI AAT'ATT Cuts at: 5 52 5926 5 Size: 47 5874 2081 Fragments arranged by size: 5874 2081 47 StuI AGG'CCT Cuts at: 1517 2777 1517 Size: 1260 6742 StyI C'CwwG_G Cuts at: 513 1065 2494 3123 3434 3700 4443 5040 5134 Size: 552 1429 629 311 266 743 597 94 Cuts at: 5134 5145 5441 513 Size: 11 296 3074 Fragments arranged by size: 3074 1429 743 629 597 552 311 296 266 94 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5033 6029 6046 6199 6384 6829 325 Size: 1255 3453 996 17 153 185 445 1498 Fragments arranged by size: 3453 1498 1255 996 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2351 3183 6248 Size: 274 80 33 51 1443 374 832 3065 Cuts at: 6248 7960 96 Size: 1712 138 Fragments arranged by size: 3065 1712 1443 832 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3990 4022 7962 7994 3990 Size: 32 3940 32 3998 Fragments arranged by size: 3998 3940 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8002 XbaI T'CTAG_A Cuts at: 4417 5338 4417 Size: 921 7081 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2106 4010 4491 2106 Size: 1904 481 5617 Fragments arranged by size: 5617 1904 481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8002 XmaI C'CCGG_G Cuts at: 1870 5327 1870 Size: 3457 4545 XmnI GAAnn'nnTTC Cuts at: 3218 6131 3218 Size: 2913 5089 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5810 276 Size: 53 83 186 936 4276 2468 Fragments arranged by size: 4276 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI