Restriction map for pFN21ASDA0230
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

Acc65I      AflII       AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbrPI       BclI        BfrI        BlnI        
BsaAI       BsaBI       BseRI       BsiEI       BsiWI       
BsmBI       BssHII      Bst1107I    BstBI       BstZ17I     
EagI        EclXI       Eco52I      EcoRI       EcoRV       
FbaI        FseI        FspI        HpaI        KpnI        
MamI        MluI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        SbfI        
ScaI        SfuI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccIII       3317  TCCGGA               T^CCGGA
AclI         1834  AACGTT               AA^CGTT
AhdI         2119  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI         8  AGCGCT               AGC^GCT
Asp700I      2316  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2119  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII         201  AGATCT               A^GATCT
BlpI          855  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      855  GCTGAGC              GC^T[ACGT]AGC
BseAI        3317  TCCGGA               T^CCGGA
BspDI        3340  ATCGAT               AT^CGAT
BspEI        3317  TCCGGA               T^CCGGA
BspLU11I     2201  ACATGT               A^CATGT
BstXI        2492  CCACGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         855  GCTGAGC              GC^T[ACGT]AGC
ClaI         3340  ATCGAT               AT^CGAT
CpoI         2827  CGGTCCG              CG^G[AT]CCG
DraI          333  TTTAAA               TTT^AAA
Eam1105I     2119  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III        8  AGCGCT               AGC^GCT
MfeI          371  CAATTG               C^AATTG
MroI         3317  TCCGGA               T^CCGGA
MunI          371  CAATTG               C^AATTG
NaeI         3677  GCCGGC               GCC^GGC
NcoI         3434  CCATGG               C^CATGG
NgoMIV       3677  GCCGGC               G^CCGGC
PaeR7I        478  CTCGAG               C^TCGAG
Psp1406I     1834  AACGTT               AA^CGTT
RsrII        2827  CGGTCCG              CG^G[AT]CCG
SacI         3412  GAGCTC               GAGCT^C
SalI         2086  GTCGAC               G^TCGAC
SfiI           75  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2176  CACCGGCG             C[GA]^CCGG[CT]G
XbaI          405  TCTAGA               T^CTAGA
XhoI          478  CTCGAG               C^TCGAG
XmnI         2316  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII        1843  GACGTC               GACGT^C
             3801  GACGTC              
AccI         2086  GTCGAC               GT^[AC][GT]AC
             3523  GTCTAC              
AcsI          330  AAATTT               [GA]^AATT[CT]
             1708  AAATTT              
AocI          996  CCTCAGG              CC^T[ACGT]AGG
             1315  CCTCAGG             
ApoI          330  AAATTT               [GA]^AATT[CT]
             1708  AAATTT              
AspI         1255  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1321  GACAGAGTC           
BbvCI        4109  CCTCAGC              CCTCAGC(-5/-2)
              252  GCTGAGG             
BcgI         2152  GCAAACCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2624  GCATGTTCTTCG        
BciVI         299  GGATAC               GTATCC(6/5)
             1986  GTATCC              
BspHI        2579  TCATGA               T^CATGA
             2678  TCATGA              
BsrBI        2610  GAGCGG               CCGCTC(-3/-3)
             3421  GAGCGG              
BssSI          46  CTCGTG               CACGAG(-5/-1)
             3649  CTCGTG              
BstAPI        150  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2543  GCAACGTGTGC         
Bsu36I        996  CCTCAGG              CC^T[ACGT]AGG
             1315  CCTCAGG             
DrdI         3514  GACTACAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4321  GACGGGCAGGTC        
Eco81I        996  CCTCAGG              CC^T[ACGT]AGG
             1315  CCTCAGG             
EcoNI        2505  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3114  CCTGGGGGAGG         
HindIII      3299  AAGCTT               A^AGCTT
             4346  AAGCTT              
PflFI        1255  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1321  GACAGAGTC           
RcaI         2579  TCATGA               T^CATGA
             2678  TCATGA              
SapI          715  GAAGAGC              GCTCTTC(1/4)
             1291  GCTCTTC             
Tth111I      1255  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1321  GACAGAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SfoI        SmaI        
SmlI        SphI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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