Restriction map for pFN21ASDA0230
 (Linear) (Six-base) MAPSORT of: KIAA0230.seq  Check: 6271  from: 1  to: 4437



KIAA0230.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     805    4342    4437 
   Size:        805    3537      95

  Fragments arranged by size: 

                3537     805      95
 
AatII G_ACGT'C

Cuts at:      0    1847    3805    4437 
   Size:       1847    1958     632

  Fragments arranged by size: 

                1958    1847     632
 
AccI GT'mk_AC

Cuts at:      0    2087    3524    4437 
   Size:       2087    1437     913

  Fragments arranged by size: 

                2087    1437     913
 
AclI AA'CG_TT

Cuts at:      0    1835    4437 
   Size:       1835    2602
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1745    3192    4101    4137    4437 
   Size:       1745    1447     909      36     300

  Fragments arranged by size: 

                1745    1447     909     300      36
 
AfeI AGC'GCT

Cuts at:      0      10    4437 
   Size:         10    4427
 
AflIII A'CryG_T

Cuts at:      0    2201    2546    2741    3548    3860    4437 
   Size:       2201     345     195     807     312     577

  Fragments arranged by size: 

                2201     807     577     345     312     195
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2124    4437 
   Size:       2124    2313
 
AleI CACnn'nnGTG

Cuts at:      0     136    1573    1603    2497    3847    4437 
   Size:        136    1437      30     894    1350     590

  Fragments arranged by size: 

                1437    1350     894     590     136      30
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     154     169    1162    3992    4437 
   Size:        154      15     993    2830     445

  Fragments arranged by size: 

                2830     993     445     154      15
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3412    3444    4437 
   Size:       3412      32     993

  Fragments arranged by size: 

                3412     993      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2139    2472    3402    4437 
   Size:       2139     333     930    1035

  Fragments arranged by size: 

                2139    1035     930     333
 
ApaI G_GGCC'C

Cuts at:      0      22    2619    3688    4437 
   Size:         22    2597    1069     749

  Fragments arranged by size: 

                2597    1069     749      22
 
ApoI r'AATT_y

Cuts at:      0     330    1708    4437 
   Size:        330    1378    2729

  Fragments arranged by size: 

                2729    1378     330
 
AvaI C'yCGr_G

Cuts at:      0      22     478     774    1654    2333    2600    3845    4437 
   Size:         22     456     296     880     679     267    1245     592

  Fragments arranged by size: 

                1245     880     679     592     456     296     267      22
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     921     954    4437 
   Size:        921      33    3483

  Fragments arranged by size: 

                3483     921      33
 
BamHI G'GATC_C

Cuts at:      0    1995    3497    3815    4437 
   Size:       1995    1502     318     622

  Fragments arranged by size: 

                1995    1502     622     318
 
BanI G'GyrC_C

Cuts at:      0      26     180    1433    1615    2236    2269    3666    4437 
   Size:         26     154    1253     182     621      33    1397     771

  Fragments arranged by size: 

                1397    1253     771     621     182     154      33      26
            
 
BanII G_rGCy'C

Cuts at:      0      22     755    1655    2619    2899    3416    3688    4437 
   Size:         22     733     900     964     280     517     272     749

  Fragments arranged by size: 

                 964     900     749     733     517     280     272      22
            
 
BbeI G_GCGC'C

Cuts at:      0      30     184    1619    2273    4437 
   Size:         30     154    1435     654    2164

  Fragments arranged by size: 

                2164    1435     654     154      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     326     871     985    3694    3956    4186    4437 
   Size:        326     545     114    2709     262     230     251

  Fragments arranged by size: 

                2709     545     326     262     251     230     114
 
BbvCI CC'TCA_GC

Cuts at:      0     253    4110    4437 
   Size:        253    3857     327

  Fragments arranged by size: 

                3857     327     253
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2141    2175    2613    2647    4437 
   Size:       2141      34     438      34    1790

  Fragments arranged by size: 

                2141    1790     438      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     293    1997    4437 
   Size:        293    1704    2440

  Fragments arranged by size: 

                2440    1704     293
 
BfrBI ATG'CAT

Cuts at:      0     150    4135    4297    4437 
   Size:        150    3985     162     140

  Fragments arranged by size: 

                3985     162     150     140
 
BglI GCCn_nnn'nGGC

Cuts at:      0      82    2798    3202    3683    4437 
   Size:         82    2716     404     481     754

  Fragments arranged by size: 

                2716     754     481     404      82
 
BglII A'GATC_T

Cuts at:      0     201    4437 
   Size:        201    4236
 
BlpI GC'TnA_GC

Cuts at:      0     856    4437 
   Size:        856    3581
 
Bme1580I G_kGCm'C

Cuts at:      0      22     173    2369    2619    3392    3688    4437 
   Size:         22     151    2196     250     773     296     749

  Fragments arranged by size: 

                2196     773     749     296     250     151      22
 
BmgBI CAC'GTC

Cuts at:      0    3861    4437 
   Size:       3861     576
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1567    3033    4064    4437 
   Size:       1567    1466    1031     373

  Fragments arranged by size: 

                1567    1466    1031     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     998    1102    2625    4437 
   Size:        181     817     104    1523    1812

  Fragments arranged by size: 

                1812    1523     817     181     104
 
Bpu10I CC'TnA_GC

Cuts at:      0     253    2506    4110    4437 
   Size:        253    2253    1604     327

  Fragments arranged by size: 

                2253    1604     327     253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2992    4033    4437 
   Size:       2992    1041     404

  Fragments arranged by size: 

                2992    1041     404
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1016    2410    3043    4013    4437 
   Size:       1016    1394     633     970     424

  Fragments arranged by size: 

                1394    1016     970     633     424
 
BsaHI Gr'CG_yC

Cuts at:      0      27     181    1312    1616    1844    2270    3802    4437 
   Size:         27     154    1131     304     228     426    1532     635

  Fragments arranged by size: 

                1532    1131     635     426     304     228     154      27
            
 
BsaWI w'CCGG_w

Cuts at:      0     503    1982    2222    3317    4437 
   Size:        503    1479     240    1095    1120

  Fragments arranged by size: 

                1479    1120    1095     503     240
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2473    2503    3412    3442    4437 
   Size:       2473      30     909      30     995

  Fragments arranged by size: 

                2473     995     909      30      30
 
BseYI C'CCAG_C

Cuts at:      0    1591    2943    3138    3681    3779    4437 
   Size:       1591    1352     195     543      98     658

  Fragments arranged by size: 

                1591    1352     658     543     195      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    1107    1576    1645    1656    2571    2842    3871 
   Size:        406     701     469      69      11     915     271    1029

Cuts at:   3871    4437 
   Size:        566

  Fragments arranged by size: 

                1029     915     701     566     469     406     271      69
                  11
 
BsiHKAI G_wGCw'C

Cuts at:      0    1237    1826    2755    2984    3416    4437 
   Size:       1237     589     929     229     432    1021

  Fragments arranged by size: 

                1237    1021     929     589     432     229
 
BsmI GAATG_Cn'

Cuts at:      0     241    1507    1586    4318    4437 
   Size:        241    1266      79    2732     119

  Fragments arranged by size: 

                2732    1266     241     119      79
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     173     755    1237    1655    1826    2369    2619 
   Size:         22     151     582     482     418     171     543     250

Cuts at:   2619    2755    2899    2984    3392    3416    3688    4437 
   Size:        136     144      85     408      24     272     749

  Fragments arranged by size: 

                 749     582     543     482     418     408     272     250
                 171     151     144     136      85      24      22
 
BspEI T'CCGG_A

Cuts at:      0    3317    4437 
   Size:       3317    1120
 
BspHI T'CATG_A

Cuts at:      0    2579    2678    4437 
   Size:       2579      99    1759

  Fragments arranged by size: 

                2579    1759      99
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     428     805    1756    2258    4317    4342    4437 
   Size:        428     377     951     502    2059      25      95

  Fragments arranged by size: 

                2059     951     502     428     377      95      25
 
BsrBI CCG'CTC

Cuts at:      0    2612    3423    4437 
   Size:       2612     811    1014

  Fragments arranged by size: 

                2612    1014     811
 
BsrDI GCAATG_nn'

Cuts at:      0     268    2044    2990    3269    4437 
   Size:        268    1776     946     279    1168

  Fragments arranged by size: 

                1776    1168     946     279     268
 
BsrFI r'CCGG_y

Cuts at:      0    1427    2177    3245    3677    4437 
   Size:       1427     750    1068     432     760

  Fragments arranged by size: 

                1427    1068     760     750     432
 
BsrGI T'GTAC_A

Cuts at:      0     350    2067    2357    4437 
   Size:        350    1717     290    2080

  Fragments arranged by size: 

                2080    1717     350     290
 
BssSI C'ACGA_G

Cuts at:      0      46    3649    4437 
   Size:         46    3603     788

  Fragments arranged by size: 

                3603     788      46
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     156    2549    4437 
   Size:        156    2393    1888

  Fragments arranged by size: 

                2393    1888     156
 
BstEII G'GTnAC_C

Cuts at:      0     990    1132    4329    4437 
   Size:        990     142    3197     108

  Fragments arranged by size: 

                3197     990     142     108
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2499    4437 
   Size:       2499    1938
 
BstYI r'GATC_y

Cuts at:      0     201     227     362    1115    1860    1995    3074    3110 
   Size:        201      26     135     753     745     135    1079      36

Cuts at:   3110    3497    3587    3815    3908    4437 
   Size:        387      90     228      93     529

  Fragments arranged by size: 

                1079     753     745     529     387     228     201     135
                 135      93      90      36      26
 
Bsu36I CC'TnA_GG

Cuts at:      0     997    1316    4437 
   Size:        997     319    3121

  Fragments arranged by size: 

                3121     997     319
 
BtgI C'CryG_G

Cuts at:      0      77     176    1111    1159    1346    1421    2492    2695 
   Size:         77      99     935      48     187      75    1071     203

Cuts at:   2695    2796    2867    2996    3150    3200    3434    3492    4057 
   Size:        101      71     129     154      50     234      58     565

Cuts at:   4057    4437 
   Size:        380

  Fragments arranged by size: 

                1071     935     565     380     234     203     187     154
                 129     101      99      77      75      71      58      50
                  48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1374    2133    4437 
   Size:       1374     759    2304

  Fragments arranged by size: 

                2304    1374     759
 
BtsI GCAGTG_nn'

Cuts at:      0     582    2464    2535    3235    4437 
   Size:        582    1882      71     700    1202

  Fragments arranged by size: 

                1882    1202     700     582      71
 
ClaI AT'CG_AT

Cuts at:      0    3341    4437 
   Size:       3341    1096
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     944     979    4437 
   Size:        944      35    3458

  Fragments arranged by size: 

                3458     944      35
 
DraI TTT'AAA

Cuts at:      0     335    4437 
   Size:        335    4102
 
DraIII CAC_nnn'GTG

Cuts at:      0    2873    2969    3447    4241    4283    4437 
   Size:       2873      96     478     794      42     154

  Fragments arranged by size: 

                2873     794     478     154      96      42
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3520    4327    4437 
   Size:       3520     807     110

  Fragments arranged by size: 

                3520     807     110
 
EaeI y'GGCC_r

Cuts at:      0       2      74     656     965    2786    2915    3216    3464 
   Size:          2      72     582     309    1821     129     301     248

Cuts at:   3464    3809    4437 
   Size:        345     628

  Fragments arranged by size: 

                1821     628     582     345     309     301     248     129
                  72       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     547     710    1298    4411    4437 
   Size:        547     163     588    3113      26

  Fragments arranged by size: 

                3113     588     547     163      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     625    3889    4437 
   Size:        625    3264     548

  Fragments arranged by size: 

                3264     625     548
 
EcoICRI GAG'CTC

Cuts at:      0    3414    4437 
   Size:       3414    1023
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     998    1102    1745    2625    3192    4101    4137 
   Size:        181     817     104     643     880     567     909      36

Cuts at:   4137    4437 
   Size:        300

  Fragments arranged by size: 

                 909     880     817     643     567     300     181     104
                  36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2509    3118    4437 
   Size:       2509     609    1319

  Fragments arranged by size: 

                2509    1319     609
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18      19     734     899    2615    3558    3660    3685 
   Size:         18       1     715     165    1716     943     102      25

Cuts at:   3685    4391    4437 
   Size:        706      46

  Fragments arranged by size: 

                1716     943     715     706     165     102      46      25
                  18       1
 
HaeII r_GCGC'y

Cuts at:      0      12      30      46     184    1094    1619    2273    2295 
   Size:         12      18      16     138     910     525     654      22

Cuts at:   2295    3649    3726    4437 
   Size:       1354      77     711

  Fragments arranged by size: 

                1354     910     711     654     525     138      77      22
                  18      16      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     519     551     757     789    2110    2142 
   Size:        352      32     135      32     206      32    1321      32

Cuts at:   2142    2473    2505    4437 
   Size:        331      32    1932

  Fragments arranged by size: 

                1932    1321     352     331     206     135      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1155    1515    1905    2088    3231    4437 
   Size:       1155     360     390     183    1143    1206

  Fragments arranged by size: 

                1206    1155    1143     390     360     183
 
HindIII A'AGCT_T

Cuts at:      0    3299    4346    4437 
   Size:       3299    1047      91

  Fragments arranged by size: 

                3299    1047      91
 
KasI G'GCGC_C

Cuts at:      0      26     180    1615    2269    4437 
   Size:         26     154    1435     654    2168

  Fragments arranged by size: 

                2168    1435     654     154      26
 
MfeI C'AATT_G

Cuts at:      0     371    4437 
   Size:        371    4066
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    2549    2762    4437 
   Size:        255    2294     213    1675

  Fragments arranged by size: 

                2294    1675     255     213
 
MscI TGG'CCA

Cuts at:      0       4    2788    3218    3811    4437 
   Size:          4    2784     430     593     626

  Fragments arranged by size: 

                2784     626     593     430       4
 
MslI CAynn'nnrTG

Cuts at:      0     136    1573    1603    2446    2452    2497    3694    3847 
   Size:        136    1437      30     843       6      45    1197     153

Cuts at:   3847    4437 
   Size:        590

  Fragments arranged by size: 

                1437    1197     843     590     153     136      45      30
                   6
 
MspA1I CmG'CkG

Cuts at:      0      32     116     655    1113    1161    1220    2621    2660 
   Size:         32      84     539     458      48      59    1401      39

Cuts at:   2660    2697    2798    2826    2841    2943    3202    3399    3480 
   Size:         37     101      28      15     102     259     197      81

Cuts at:   3480    3783    3899    4437 
   Size:        303     116     538

  Fragments arranged by size: 

                1401     539     538     458     303     259     197     116
                 102     101      84      81      59      48      39      37
                  32      28      15
 
NaeI GCC'GGC

Cuts at:      0    3679    4437 
   Size:       3679     758
 
NarI GG'CG_CC

Cuts at:      0      27     181    1616    2270    4437 
   Size:         27     154    1435     654    2167

  Fragments arranged by size: 

                2167    1435     654     154      27
 
NcoI C'CATG_G

Cuts at:      0    3434    4437 
   Size:       3434    1003
 
NgoMIV G'CCGG_C

Cuts at:      0    3677    4437 
   Size:       3677     760
 
NsiI A_TGCA'T

Cuts at:      0     152    4137    4299    4437 
   Size:        152    3985     162     138

  Fragments arranged by size: 

                3985     162     152     138
 
NspI r_CATG'y

Cuts at:      0     150    2068    2205    2451    2628    2880    2961    4301 
   Size:        150    1918     137     246     177     252      81    1340

Cuts at:   4301    4437 
   Size:        136

  Fragments arranged by size: 

                1918    1340     252     246     177     150     137     136
                  81
 
PasI CC'CwG_GG

Cuts at:      0    1644    3760    3914    4387    4437 
   Size:       1644    2116     154     473      50

  Fragments arranged by size: 

                2116    1644     473     154      50
 
PciI A'CATG_T

Cuts at:      0    2201    4437 
   Size:       2201    2236
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     951    1347    1670    2472    4437 
   Size:        951     396     323     802    1965

  Fragments arranged by size: 

                1965     951     802     396     323
 
PfoI T'CCnGG_A

Cuts at:      0    1120    2471    2830    3590    4437 
   Size:       1120    1351     359     760     847

  Fragments arranged by size: 

                1351    1120     847     760     359
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2179    2211    3412    3444    4148    4180    4437 
   Size:       2179      32    1201      32     704      32     257

  Fragments arranged by size: 

                2179    1201     704     257      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     899    3558    3660    4437 
   Size:        899    2659     102     777

  Fragments arranged by size: 

                2659     899     777     102
 
PshAI GACnn'nnGTC

Cuts at:      0    1766    1847    1946    4437 
   Size:       1766      81      99    2491

  Fragments arranged by size: 

                2491    1766      99      81
 
PspOMI G'GGCC_C

Cuts at:      0      18    2615    3684    4437 
   Size:         18    2597    1069     753

  Fragments arranged by size: 

                2597    1069     753      18
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     772     804    2674    2706    4437 
   Size:        772      32    1870      32    1731

  Fragments arranged by size: 

                1870    1731     772      32      32
 
PstI C_TGCA'G

Cuts at:      0     802    1761    2255    2542    4437 
   Size:        802     959     494     287    1895

  Fragments arranged by size: 

                1895     959     802     494     287
 
PvuII CAG'CTG

Cuts at:      0    2943    3399    3783    3899    4437 
   Size:       2943     456     384     116     538

  Fragments arranged by size: 

                2943     538     456     384     116
 
RsrII CG'GwC_CG

Cuts at:      0    2828    4437 
   Size:       2828    1609
 
SacI G_AGCT'C

Cuts at:      0    3416    4437 
   Size:       3416    1021
 
SacII CC_GC'GG

Cuts at:      0    1114    1162    2698    2799    3203    4437 
   Size:       1114      48    1536     101     404    1234

  Fragments arranged by size: 

                1536    1234    1114     404     101      48
 
SalI G'TCGA_C

Cuts at:      0    2086    4437 
   Size:       2086    2351
 
SanDI GG'GwC_CC

Cuts at:      0     899    4437 
   Size:        899    3538
 
SapI GCTCTTCn'nnn_

Cuts at:      0     710    1298    4437 
   Size:        710     588    3139

  Fragments arranged by size: 

                3139     710     588
 
SexAI A'CCwGG_T

Cuts at:      0    1436    2120    3662    4437 
   Size:       1436     684    1542     775

  Fragments arranged by size: 

                1542    1436     775     684
 
SfcI C'TryA_G

Cuts at:      0     798    1757    2251    2538    3516    3954    4437 
   Size:        798     959     494     287     978     438     483

  Fragments arranged by size: 

                 978     959     798     494     483     438     287
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      82    4437 
   Size:         82    4355
 
SfoI GGC'GCC

Cuts at:      0      28     182    1617    2271    4437 
   Size:         28     154    1435     654    2166

  Fragments arranged by size: 

                2166    1435     654     154      28
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2177    4437 
   Size:       2177    2260
 
SmaI CCC'GGG

Cuts at:      0      24    2602    3847    4437 
   Size:         24    2578    1245     590

  Fragments arranged by size: 

                2578    1245     590      24
 
SmlI C'TyrA_G

Cuts at:      0     478    3007    4012    4437 
   Size:        478    2529    1005     425

  Fragments arranged by size: 

                2529    1005     478     425
 
SphI G_CATG'C

Cuts at:      0     150    2880    2961    4301    4437 
   Size:        150    2730      81    1340     136

  Fragments arranged by size: 

                2730    1340     150     136      81
 
StyI C'CwwG_G

Cuts at:      0    1367    1400    3434    4437 
   Size:       1367      33    2034    1003

  Fragments arranged by size: 

                2034    1367    1003      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2458    3055    4437 
   Size:       2458     597    1382

  Fragments arranged by size: 

                2458    1382     597
 
TatI w'GTAC_w

Cuts at:      0     350     788    2067    2357    4437 
   Size:        350     438    1279     290    2080

  Fragments arranged by size: 

                2080    1279     438     350     290
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     291     323     680     712    1116    1148    1342    1374 
   Size:        291      32     357      32     404      32     194      32

Cuts at:   1374    3369    3401    3730    3762    4437 
   Size:       1995      32     329      32     675

  Fragments arranged by size: 

                1995     675     404     357     329     291     194      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1258    1324    4437 
   Size:       1258      66    3113

  Fragments arranged by size: 

                3113    1258      66
 
XbaI T'CTAG_A

Cuts at:      0     405    4437 
   Size:        405    4032
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1667    2385    2940    3060    4242    4437 
   Size:       1667     718     555     120    1182     195

  Fragments arranged by size: 

                1667    1182     718     555     195     120
 
XhoI C'TCGA_G

Cuts at:      0     478    4437 
   Size:        478    3959
 
XmaI C'CCGG_G

Cuts at:      0      22    2600    3845    4437 
   Size:         22    2578    1245     592

  Fragments arranged by size: 

                2578    1245     592      22
 
XmnI GAAnn'nnTTC

Cuts at:      0    2320    4437 
   Size:       2320    2117
 
ZraI GAC'GTC

Cuts at:      0    1845    3803    4437 
   Size:       1845    1958     634

  Fragments arranged by size: 

                1958    1845     634
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII  HindIII     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI     PsrI
     PstI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I    AflII     AgeI    ApaLI     AscI     AseI    AsiSI    AvrII
     BclI     BmtI     BplI    BsaAI    BsaBI    BseRI    BsiEI    BsiWI
    BsmBI   BssHII    BstBI  BstZ17I     EagI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KpnI     MluI     NdeI     NheI
     NotI     NruI     PacI     PmeI     PmlI     PsiI    PspXI     PvuI
     SbfI     ScaI     SgfI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI
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