Restriction map for
pFN21ASDA0224
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AclI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BciVI BfaI BfrI
BlnI BsaI BsiWI BsmI Bsp1407I
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 2427 GACGTC GACGT^C
AccIII 311 TCCGGA T^CCGGA
AspI 3199 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1236 GGTGCC G^G[CT][GA]CC
BbvCI 274 GCTGAGG CCTCAGC(-5/-2)
BglI 3581 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2871 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 311 TCCGGA [AT]^CCGG[AT]
BseAI 311 TCCGGA T^CCGGA
BsiEI 966 CGATCG CG[GA][CT]^CG
BspEI 311 TCCGGA T^CCGGA
BspHI 3368 TCATGA T^CATGA
BsrDI 1326 CATTGC GCAATG(2/0)
BsrFI 2569 GCCGGC [GA]^CCGG[CT]
Cfr10I 2569 GCCGGC [GA]^CCGG[CT]
EcoNI 1812 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MamI 2871 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 311 TCCGGA T^CCGGA
MseI 2482 TTAA T^TAA
NaeI 2569 GCCGGC GCC^GGC
NgoMIV 2569 GCCGGC G^CCGGC
PaeR7I 749 CTCGAG C^TCGAG
PflFI 3199 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2711 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 966 CGATCG CGAT^CG
RcaI 3368 TCATGA T^CATGA
SapI 1453 GAAGAGC GCTCTTC(1/4)
ScaI 779 AGTACT AGT^ACT
SphI 1142 GCATGC GCATG^C
SspI 2416 AATATT AAT^ATT
Tru9I 2482 TTAA T^TAA
Tth111I 3199 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 749 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 636 GTCTAC GT^[AC][GT]AC
3600 GTCTAC
AcsI 908 GAATTT [GA]^AATT[CT]
1552 GAATTT
Alw44I 1225 GTGCAC G^TGCAC
2200 GTGCAC
Aor51HI 1124 AGCGCT AGC^GCT
1967 AGCGCT
ApaLI 1225 GTGCAC G^TGCAC
2200 GTGCAC
ApoI 908 GAATTT [GA]^AATT[CT]
1552 GAATTT
BamHI 1892 GGATCC G^GATCC
2783 GGATCC
BcgI 960 GCAAGCCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1646 GCATCGTGATCG
BclI 1871 TGATCA T^GATCA
2982 TGATCA
BglII 2522 AGATCT A^GATCT
3608 AGATCT
BlpI 1093 GCTCAGC GC^T[ACGT]AGC
1387 GCTCAGC
Bpu1102I 1093 GCTCAGC GC^T[ACGT]AGC
1387 GCTCAGC
BspDI 32 ATCGAT AT^CGAT
2461 ATCGAT
BstEII 289 GGTGACC G^GT[ACGT]ACC
2262 GGTGACC
BstPI 289 GGTGACC G^GT[ACGT]ACC
2262 GGTGACC
CelII 1093 GCTCAGC GC^T[ACGT]AGC
1387 GCTCAGC
ClaI 32 ATCGAT AT^CGAT
2461 ATCGAT
DsaI 3512 CCATGG C^C[GA][CT]GG
3644 CCATGG
Eco47III 1124 AGCGCT AGC^GCT
1967 AGCGCT
EcoO65I 289 GGTGACC G^GT[ACGT]ACC
2262 GGTGACC
FbaI 1871 TGATCA T^GATCA
2982 TGATCA
HincII 2131 GTTGAC GT[CT]^[GA]AC
2440 GTTGAC
HindII 2131 GTTGAC GT[CT]^[GA]AC
2440 GTTGAC
NcoI 3512 CCATGG C^CATGG
3644 CCATGG
SacI 2629 GAGCTC GAGCT^C
3134 GAGCTC
XcmI 1307 CCACTTCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2498 CCAGGATTGGCATGG
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII