Restriction map for
pFN21ASDA0224
(Circular) (Six-base) MAPSORT of: pFN21ASDA0224.seq Check: 5876 from: 1 to: 8410
pFN21ASDA0224 8410 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3093 4210 3093
Size: 1117 7293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4454 6220 278
Size: 53 83 186 936 2918 1766 2468
Fragments arranged by size:
2918 2468 1766 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 5624 5753 1937
Size: 723 2964 129 4594
Fragments arranged by size:
4594 2964 723 129
Acc65I G'GTAC_C
Cuts at: 5731 5731
Size: 8410
AclI AA'CG_TT
Cuts at: 1563 6537 6910 1563
Size: 4974 373 3063
Fragments arranged by size:
4974 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3864 4076 4150 6473 7857 8125 1632
Size: 2232 212 74 2323 1384 268 1917
Fragments arranged by size:
2323 2232 1917 1384 268 212 74
AfeI AGC'GCT
Cuts at: 3149 3992 3149
Size: 843 7567
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8213
AflIII A'CryG_T
Cuts at: 1203 7309 8286 1203
Size: 6106 977 1327
Fragments arranged by size:
6106 1327 977
AgeI A'CCGG_T
Cuts at: 7241 7241
Size: 8410
AhdI GACnn_n'nnGTC
Cuts at: 7139 7139
Size: 8410
AleI CACnn'nnGTG
Cuts at: 1166 3960 4007 4667 1166
Size: 2794 47 660 4909
Fragments arranged by size:
4909 2794 660 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3552 5327 1184
Size: 2368 1775 4267
Fragments arranged by size:
4267 2368 1775
AlwNI CAG_nnn'CTG
Cuts at: 3085 3616 3643 3727 4738 6153 7725 3085
Size: 531 27 84 1011 1415 1572 3770
Fragments arranged by size:
3770 1572 1415 1011 531 84 27
ApaI G_GGCC'C
Cuts at: 4240 4607 4819 5459 4240
Size: 367 212 640 7191
Fragments arranged by size:
7191 640 367 212
ApaLI G'TGCA_C
Cuts at: 3248 4223 6467 7623 3248
Size: 975 2244 1156 4035
Fragments arranged by size:
4035 2244 1156 975
ApoI r'AATT_y
Cuts at: 1493 2931 3575 5719 5945 6097 8206 1493
Size: 1438 644 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 1438 644 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6964 160
Size: 872 5932 1606
Fragments arranged by size:
5932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8410
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 2612 2772 4673 4724 4811 5735
Size: 73 418 251 160 1901 51 87 924
Cuts at: 5735 1870
Size: 4545
Fragments arranged by size:
4545 1901 924 418 251 160 87 73
51
BamHI G'GATC_C
Cuts at: 3915 4806 5740 6107 3915
Size: 891 934 367 6218
Fragments arranged by size:
6218 934 891 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3259 5731 7186 618
Size: 325 217 627 1472 2472 1455 1842
Fragments arranged by size:
2472 1842 1472 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2621 4240 4607 4656 4819 5161 5459 5578
Size: 1230 1619 367 49 163 342 298 119
Cuts at: 5578 5667 5729 1391
Size: 89 62 4072
Fragments arranged by size:
4072 1619 1230 367 342 298 163 119
89 62 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 2872 2914 3280 3876 4504 928
Size: 971 229 744 42 366 596 628 4834
Fragments arranged by size:
4834 971 744 628 596 366 229 42
BbvCI CC'TCA_GC
Cuts at: 2298 2298
Size: 8410
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2972 3006 3658 3692 6601 6635 1903
Size: 34 1035 34 652 34 2909 34 3678
Fragments arranged by size:
3678 2909 1035 652 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6302 7512 6302
Size: 1210 7200
BclI T'GATC_A
Cuts at: 1548 3894 5005 1548
Size: 2346 1111 4953
Fragments arranged by size:
4953 2346 1111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5610 7021 136
Size: 107 122 71 631 605 3938 1411 1525
Fragments arranged by size:
3938 1525 1411 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4545 5631 8405 4545
Size: 1086 2774 4550
Fragments arranged by size:
4550 2774 1086
BlpI GC'TnA_GC
Cuts at: 3117 3411 5827 3117
Size: 294 2416 5700
Fragments arranged by size:
5700 2416 294
Bme1580I G_kGCm'C
Cuts at: 1790 3252 4227 4240 4607 4674 4819 5459 6471
Size: 1462 975 13 367 67 145 640 1012
Cuts at: 6471 7627 1790
Size: 1156 2573
Fragments arranged by size:
2573 1462 1156 1012 975 640 367 145
67 13
BmgBI CAC'GTC
Cuts at: 1318 2912 4009 1318
Size: 1594 1097 5719
Fragments arranged by size:
5719 1594 1097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 2275 3433
Size: 416 53 83 399 24 760 98 1158
Cuts at: 3433 3455 5227 6141 7099 442
Size: 22 1772 914 958 1753
Fragments arranged by size:
1772 1753 1158 958 914 760 416 399
98 83 53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 3950 4306 5180 5545 2062
Size: 1888 356 874 365 4927
Fragments arranged by size:
4927 1888 874 365 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3215 3620 4263 4334 4343 4364 4389 5625
Size: 1976 405 643 71 9 21 25 1236
Cuts at: 5625 7070 1239
Size: 1445 2579
Fragments arranged by size:
2579 1976 1445 1236 643 405 71 25
21 9
Bpu10I CC'TnA_GC
Cuts at: 2298 5127 2298
Size: 2829 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2680 4700 5150 5258 6535 7400 7698 2680
Size: 2020 450 108 1277 865 298 3392
Fragments arranged by size:
3392 2020 1277 865 450 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7073 882
Size: 617 5574 2219
Fragments arranged by size:
5574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3064 4192 5293 5707 6201 493
Size: 711 1860 1128 1101 414 494 2702
Fragments arranged by size:
2702 1860 1128 1101 711 494 414
BsaBI GATnn'nnATC
Cuts at: 4898 8404 4898
Size: 3506 4904
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2729 3462 4451 6217
Size: 53 83 186 936 1196 733 989 1766
Cuts at: 6217 6599 7234 275
Size: 382 635 1451
Fragments arranged by size:
1766 1451 1196 989 936 733 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 6843 7241 7515 7662 8073 2334
Size: 4509 398 274 147 411 2671
Fragments arranged by size:
4509 2671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3977 4007 1178
Size: 30 165 30 2574 30 5581
Fragments arranged by size:
5581 2574 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2242 2683 2930 2963 2966 3040 3082
Size: 1039 17 441 247 33 3 74 42
Cuts at: 3082 3857 5009 5012 5555 5558 5576 1186
Size: 775 1152 3 543 3 18 4020
Fragments arranged by size:
4020 1152 1039 775 543 441 247 74
42 33 18 17 3 3 3
BseYI C'CCAG_C
Cuts at: 3150 3771 4963 5452 7613 8109 3150
Size: 621 1192 489 2161 496 3451
Fragments arranged by size:
3451 2161 1192 621 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3653 3859 4751 5201 1163
Size: 573 1917 206 892 450 4372
Fragments arranged by size:
4372 1917 892 573 450 206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 5860 6621 7649 1540
Size: 275 186 991 2868 761 1028 2301
Fragments arranged by size:
2868 2301 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3252 4014 4227 4656 5161 6471 6556 7627 3252
Size: 762 213 429 505 1310 85 1071 4035
Fragments arranged by size:
4035 1310 1071 762 505 429 213 85
BsmI GAATG_Cn'
Cuts at: 5921 6014 5921
Size: 93 8317
BsmBI CGTCTCn'nnnn_
Cuts at: 3683 4442 4458 3683
Size: 759 16 7635
Fragments arranged by size:
7635 759 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2621 3252 4014 4227 4240 4607 4656
Size: 399 831 631 762 213 13 367 49
Cuts at: 4656 4674 4819 5161 5459 5578 5667 5729 6471
Size: 18 145 342 298 119 89 62 742
Cuts at: 6471 6556 7627 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 831 762 742 631 399 367
342 298 213 145 119 89 85 62
49 18 13
BspEI T'CCGG_A
Cuts at: 2334 8073 2334
Size: 5739 2671
BspHI T'CATG_A
Cuts at: 5391 6299 8029 5391
Size: 908 1730 5772
Fragments arranged by size:
5772 1730 908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3093 3624 3735 4210 4746 5076 5765 844
Size: 2249 531 111 475 536 330 689 3489
Fragments arranged by size:
3489 2249 689 536 531 475 330 111
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 2437 2539 2752 2956 6297 1394
Size: 429 608 6 102 213 204 3341 3507
Fragments arranged by size:
3507 3341 608 429 213 204 102 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 3348 6905 7079 65
Size: 1183 2100 3557 174 1396
Fragments arranged by size:
3557 2100 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4592 7054 7241 1700
Size: 110 2782 2462 187 2869
Fragments arranged by size:
2869 2782 2462 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8410
BssSI C'ACGA_G
Cuts at: 3986 5203 5381 6470 7482 3986
Size: 1217 178 1089 1012 4914
Fragments arranged by size:
4914 1217 1089 1012 178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3085 3616 3643 3727 4216 4738 794
Size: 2291 531 27 84 489 522 4466
Fragments arranged by size:
4466 2291 531 522 489 84 27
BstBI TT'CG_AA
Cuts at: 2007 7214 2007
Size: 5207 3203
BstEII G'GTnAC_C
Cuts at: 1184 2312 4285 1184
Size: 1128 1973 5309
Fragments arranged by size:
5309 1973 1128
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 3181 3799 3973 4136 2387
Size: 794 618 174 163 6661
Fragments arranged by size:
6661 794 618 174 163
BstYI r'GATC_y
Cuts at: 1972 3915 4545 4677 4806 4932 5631 5740 6107
Size: 1943 630 132 129 126 699 109 367
Cuts at: 6107 6492 6509 7961 8047 8059 8405 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1943 1452 699 630 385 367 346
132 129 126 109 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 5535 5667 513
Size: 552 957 3513 132 3256
Fragments arranged by size:
3513 3256 957 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7671
Fragments arranged by size:
7671 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 3577 3636 4209 5078 5420 5620 5926 6720
Size: 608 59 573 869 342 200 306 794
Cuts at: 6720 6740 2969
Size: 20 4639
Fragments arranged by size:
4639 869 794 608 573 342 306 200
59 20
ClaI AT'CG_AT
Cuts at: 1554 2056 4485 1554
Size: 502 2429 5479
Fragments arranged by size:
5479 2429 502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4423 4458 4813 4848 529
Size: 35 3859 35 355 35 4091
Fragments arranged by size:
4091 3859 355 35 35 35
DraI TTT'AAA
Cuts at: 5714 5714
Size: 8410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7417 809
Size: 507 6101 1802
Fragments arranged by size:
6101 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2323 3228
Size: 54 1425 50 275 18 288 205 905
Cuts at: 3228 3342 3801 4138 4267 4695 5556 5857 6746
Size: 114 459 337 129 428 861 301 889
Cuts at: 6746 8375 8
Size: 1629 43
Fragments arranged by size:
1629 1425 905 889 861 459 428 337
301 288 275 205 129 114 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5857 1537
Size: 275 4045 4090
Fragments arranged by size:
4090 4045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2298 2394 2667 3210 3471 3545 3813
Size: 912 33 96 273 543 261 74 268
Cuts at: 3813 4169 6340 1353
Size: 356 2171 3423
Fragments arranged by size:
3423 2171 912 543 356 273 268 261
96 74 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5462 6997 7371 7517 387
Size: 1064 138 3873 1535 374 146 1280
Fragments arranged by size:
3873 1535 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4654 5159 4654
Size: 505 7905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3215 3620 3864 4076 4150 4263 4334
Size: 393 1583 405 244 212 74 113 71
Cuts at: 4334 4343 4364 4389 5625 6473 7070 7857 8125
Size: 9 21 25 1236 848 597 787 268
Cuts at: 8125 1239
Size: 1524
Fragments arranged by size:
1583 1524 1236 848 787 597 405 393
268 244 212 113 74 71 25 21
9
EcoNI CCTnn'n_nnAGG
Cuts at: 3839 3839
Size: 8410
EcoO109I rG'GnC_Cy
Cuts at: 4236 4237 4735 4815 4816 5456 4236
Size: 1 498 80 1 640 7190
Fragments arranged by size:
7190 640 498 80 1 1
EcoRI G'AATT_C
Cuts at: 5719 6097 5719
Size: 378 8032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4231 4231
Size: 8410
FspI TGC'GCA
Cuts at: 6916 6916
Size: 8410
HaeII r_GCGC'y
Cuts at: 1678 2704 3151 3994 4048 4641 5555 7557 1678
Size: 1026 447 843 54 593 914 2002 2531
Fragments arranged by size:
2531 2002 1026 914 843 593 447 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2567 2599 5623
Size: 32 135 32 788 32 175 32 3024
Cuts at: 5623 5655 7051 7083 7125 7157 1373
Size: 32 1396 32 42 32 2626
Fragments arranged by size:
3024 2626 1396 788 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4156 4465 5754 6000 669
Size: 988 281 2218 309 1289 246 3079
Fragments arranged by size:
3079 2218 1289 988 309 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8410
HpaI GTT'AAC
Cuts at: 6000 6000
Size: 8410
KpnI G_GTAC'C
Cuts at: 5735 5735
Size: 8410
MfeI C'AATT_G
Cuts at: 6009 6009
Size: 8410
MluI A'CGCG_T
Cuts at: 8286 8286
Size: 8410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7524 7708 1305
Size: 6219 184 2007
Fragments arranged by size:
6219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2325 3344 3803 4269 4697
Size: 54 1425 343 493 1019 459 466 428
Cuts at: 4697 10
Size: 3723
Fragments arranged by size:
3723 1425 1019 493 466 459 428 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2385 3735 3797 3960 3971 4007 4283
Size: 648 1219 1350 62 163 11 36 276
Cuts at: 4283 4667 5177 5309 6368 6727 6886 518
Size: 384 510 132 1059 359 159 2042
Fragments arranged by size:
2042 1350 1219 1059 648 510 384 359
276 163 159 132 62 36 11
MspA1I CmG'CkG
Cuts at: 1606 3040 3121 3715 4366 4579 4873 5188 5426
Size: 1434 81 594 651 213 294 315 238
Cuts at: 5426 5569 5826 6503 7651 7896 1606
Size: 143 257 677 1148 245 2120
Fragments arranged by size:
2120 1434 1148 677 651 594 315 294
257 245 238 213 143 81
NaeI GCC'GGC
Cuts at: 4594 4594
Size: 8410
NcoI C'CATG_G
Cuts at: 513 1065 2022 5535 5667 513
Size: 552 957 3513 132 3256
Fragments arranged by size:
3513 3256 957 552 132
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8410
NgoMIV G'CCGG_C
Cuts at: 4592 4592
Size: 8410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8410
NotI GC'GGCC_GC
Cuts at: 5857 5857
Size: 8410
NspI r_CATG'y
Cuts at: 1132 1229 2135 3169 4783 5768 7313 1132
Size: 97 906 1034 1614 985 1545 2229
Fragments arranged by size:
2229 1614 1545 1034 985 906 97
PasI CC'CwG_GG
Cuts at: 1348 5608 1348
Size: 4260 4150
PciI A'CATG_T
Cuts at: 7309 7309
Size: 8410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 3193 4303 4756 5100 1108
Size: 951 1134 1110 453 344 4418
Fragments arranged by size:
4418 1134 1110 951 453 344
PfoI T'CCnGG_A
Cuts at: 3597 3936 4026 5013 5145 3597
Size: 339 90 987 132 6862
Fragments arranged by size:
6862 987 339 132 90
PmeI GTTT'AAAC
Cuts at: 5714 5714
Size: 8410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6479 6511 7992 8024 6479
Size: 32 1481 32 6865
Fragments arranged by size:
6865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4735 4735
Size: 8410
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8410
PsiI TTA'TAA
Cuts at: 5980 5980
Size: 8410
PspOMI G'GGCC_C
Cuts at: 4236 4603 4815 5455 4236
Size: 367 212 640 7191
Fragments arranged by size:
7191 640 367 212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5072 5104 5072
Size: 32 8378
PstI C_TGCA'G
Cuts at: 830 4204 4545 4746 4925 5073 5762 830
Size: 3374 341 201 179 148 689 3478
Fragments arranged by size:
3478 3374 689 341 201 179 148
PvuI CG_AT'CG
Cuts at: 2001 2992 2001
Size: 991 7419
PvuII CAG'CTG
Cuts at: 3715 4366 5569 3715
Size: 651 1203 6556
Fragments arranged by size:
6556 1203 651
SacI G_AGCT'C
Cuts at: 4656 5161 4656
Size: 505 7905
SalI G'TCGA_C
Cuts at: 1936 5752 1936
Size: 3816 4594
SapI GCTCTTCn'nnn_
Cuts at: 3471 3471
Size: 8410
SbfI CC_TGCA'GG
Cuts at: 5762 5762
Size: 8410
ScaI AGT'ACT
Cuts at: 2804 6658 8370 2804
Size: 3854 1712 2844
Fragments arranged by size:
3854 2844 1712
SfcI C'TryA_G
Cuts at: 826 1045 3517 3958 4200 4541 4742 4921 4975
Size: 219 2472 441 242 341 201 179 54
Cuts at: 4975 5069 5467 5758 6893 7574 7765 826
Size: 94 398 291 1135 681 191 1471
Fragments arranged by size:
2472 1471 1135 681 441 398 341 291
242 219 201 191 179 94 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8410
SmaI CCC'GGG
Cuts at: 1872 5737 1872
Size: 3865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2695 2772 4715 5165 5273 6514
Size: 197 926 752 77 1943 450 108 1241
Cuts at: 6514 7415 7677 820
Size: 901 262 1553
Fragments arranged by size:
1943 1553 1241 926 901 752 450 262
197 108 77
SnaBI TAC'GTA
Cuts at: 493 5707 6201 493
Size: 5214 494 2702
Fragments arranged by size:
5214 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8410
SphI G_CATG'C
Cuts at: 1132 3169 5768 1132
Size: 2037 2599 3774
Fragments arranged by size:
3774 2599 2037
SspI AAT'ATT
Cuts at: 5 52 4441 6334 5
Size: 47 4389 1893 2081
Fragments arranged by size:
4389 2081 1893 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8410
StyI C'CwwG_G
Cuts at: 513 1065 2022 3231 3835 4983 5400 5535 5667
Size: 552 957 1209 604 1148 417 135 132
Cuts at: 5667 5849 513
Size: 182 3074
Fragments arranged by size:
3074 1209 1148 957 604 552 417 182
135 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 3753 4646 4948 6437 6454 6607
Size: 1255 763 1410 893 302 1489 17 153
Cuts at: 6607 6792 7237 325
Size: 185 445 1498
Fragments arranged by size:
1498 1489 1410 1255 893 763 445 302
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 6656 8368
Size: 274 80 33 51 1443 825 3854 1712
Cuts at: 8368 96
Size: 138
Fragments arranged by size:
3854 1712 1443 825 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 3977 4009 5423 5455 8370 8402 2861
Size: 32 1084 32 1414 32 2915 32 2869
Fragments arranged by size:
2915 2869 1414 1084 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5225 1316
Size: 3909 4501
XbaI T'CTAG_A
Cuts at: 5746 5746
Size: 8410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3337 4528 3337
Size: 1191 7219
XhoI C'TCGA_G
Cuts at: 1943 2772 1943
Size: 829 7581
XmaI C'CCGG_G
Cuts at: 1870 5735 1870
Size: 3865 4545
XmnI GAAnn'nnTTC
Cuts at: 6539 6539
Size: 8410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4452 6218 276
Size: 53 83 186 936 2918 1766 2468
Fragments arranged by size:
2918 2468 1766 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI