Restriction map for pFN21ASDA0224
 (Linear) (Six-base) MAPSORT of: KIAA0224.seq  Check: 991  from: 1  to: 3681



KIAA0224.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1070    2187    3681 
   Size:       1070    1117    1494

  Fragments arranged by size: 

                1494    1117    1070
 
AatII G_ACGT'C

Cuts at:      0    2431    3681 
   Size:       2431    1250
 
AccI GT'mk_AC

Cuts at:      0     637    3601    3681 
   Size:        637    2964      80

  Fragments arranged by size: 

                2964     637      80
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1841    2053    2127    3681 
   Size:       1841     212      74    1554

  Fragments arranged by size: 

                1841    1554     212      74
 
AfeI AGC'GCT

Cuts at:      0    1126    1969    3681 
   Size:       1126     843    1712

  Fragments arranged by size: 

                1712    1126     843
 
AleI CACnn'nnGTG

Cuts at:      0    1937    1984    2644    3681 
   Size:       1937      47     660    1037

  Fragments arranged by size: 

                1937    1037     660      47
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1529    3304    3681 
   Size:       1529    1775     377

  Fragments arranged by size: 

                1775    1529     377
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1062    1593    1620    1704    2715    3681 
   Size:       1062     531      27      84    1011     966

  Fragments arranged by size: 

                1062    1011     966     531      84      27
 
ApaI G_GGCC'C

Cuts at:      0    2217    2584    2796    3436    3681 
   Size:       2217     367     212     640     245

  Fragments arranged by size: 

                2217     640     367     245     212
 
ApaLI G'TGCA_C

Cuts at:      0    1225    2200    3681 
   Size:       1225     975    1481

  Fragments arranged by size: 

                1481    1225     975
 
ApoI r'AATT_y

Cuts at:      0     908    1552    3681 
   Size:        908     644    2129

  Fragments arranged by size: 

                2129     908     644
 
AvaI C'yCGr_G

Cuts at:      0     338     589     749    2650    2701    2788    3681 
   Size:        338     251     160    1901      51      87     893

  Fragments arranged by size: 

                1901     893     338     251     160      87      51
 
BamHI G'GATC_C

Cuts at:      0    1892    2783    3681 
   Size:       1892     891     898

  Fragments arranged by size: 

                1892     898     891
 
BanI G'GyrC_C

Cuts at:      0    1236    3681 
   Size:       1236    2445
 
BanII G_rGCy'C

Cuts at:      0     598    2217    2584    2633    2796    3138    3436    3555 
   Size:        598    1619     367      49     163     342     298     119

Cuts at:   3555    3644    3681 
   Size:         89      37

  Fragments arranged by size: 

                1619     598     367     342     298     163     119      89
                  49      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     105     849     891    1257    1853    2481    3681 
   Size:        105     744      42     366     596     628    1200

  Fragments arranged by size: 

                1200     744     628     596     366     105      42
 
BbvCI CC'TCA_GC

Cuts at:      0     275    3681 
   Size:        275    3406
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     949     983    1635    1669    3681 
   Size:        949      34     652      34    2012

  Fragments arranged by size: 

                2012     949     652      34      34
 
BclI T'GATC_A

Cuts at:      0    1871    2982    3681 
   Size:       1871    1111     699

  Fragments arranged by size: 

                1871    1111     699
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3587    3681 
   Size:       3587      94
 
BglII A'GATC_T

Cuts at:      0    2522    3608    3681 
   Size:       2522    1086      73

  Fragments arranged by size: 

                2522    1086      73
 
BlpI GC'TnA_GC

Cuts at:      0    1094    1388    3681 
   Size:       1094     294    2293

  Fragments arranged by size: 

                2293    1094     294
 
Bme1580I G_kGCm'C

Cuts at:      0    1229    2204    2217    2584    2651    2796    3436    3681 
   Size:       1229     975      13     367      67     145     640     245

  Fragments arranged by size: 

                1229     975     640     367     245     145      67      13
            
 
BmgBI CAC'GTC

Cuts at:      0     889    1986    3681 
   Size:        889    1097    1695

  Fragments arranged by size: 

                1695    1097     889
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     154     252    1410    1432    3204    3681 
   Size:        154      98    1158      22    1772     477

  Fragments arranged by size: 

                1772    1158     477     154      98      22
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      39    1927    2283    3157    3522    3681 
   Size:         39    1888     356     874     365     159

  Fragments arranged by size: 

                1888     874     365     356     159      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1192    1597    2240    2311    2320    2341    2366    3602 
   Size:       1192     405     643      71       9      21      25    1236

Cuts at:   3602    3681 
   Size:         79

  Fragments arranged by size: 

                1236    1192     643     405      79      71      25      21
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0     275    3104    3681 
   Size:        275    2829     577

  Fragments arranged by size: 

                2829     577     275
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     657    2677    3127    3235    3681 
   Size:        657    2020     450     108     446

  Fragments arranged by size: 

                2020     657     450     446     108
 
BsaAI yAC'GTr

Cuts at:      0    1041    2169    3270    3681 
   Size:       1041    1128    1101     411

  Fragments arranged by size: 

                1128    1101    1041     411
 
BsaBI GATnn'nnATC

Cuts at:      0    2875    3681 
   Size:       2875     806
 
BsaHI Gr'CG_yC

Cuts at:      0     706    1439    2428    3681 
   Size:        706     733     989    1253

  Fragments arranged by size: 

                1253     989     733     706
 
BsaWI w'CCGG_w

Cuts at:      0     311    3681 
   Size:        311    3370
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1954    1984    3681 
   Size:       1954      30    1697

  Fragments arranged by size: 

                1954    1697      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     219     660     907     940     943    1017    1059 
   Size:        202      17     441     247      33       3      74      42

Cuts at:   1059    1834    2986    2989    3532    3535    3553    3681 
   Size:        775    1152       3     543       3      18     128

  Fragments arranged by size: 

                1152     775     543     441     247     202     128      74
                  42      33      18      17       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1127    1748    2940    3429    3681 
   Size:       1127     621    1192     489     252

  Fragments arranged by size: 

                1192    1127     621     489     252
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1630    1836    2728    3178    3681 
   Size:       1630     206     892     450     503

  Fragments arranged by size: 

                1630     892     503     450     206
 
BsiEI CG_ry'CG

Cuts at:      0     969    3681 
   Size:        969    2712
 
BsiHKAI G_wGCw'C

Cuts at:      0    1229    1991    2204    2633    3138    3681 
   Size:       1229     762     213     429     505     543

  Fragments arranged by size: 

                1229     762     543     505     429     213
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1660    2419    2435    3681 
   Size:       1660     759      16    1246

  Fragments arranged by size: 

                1660    1246     759      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     598    1229    1991    2204    2217    2584    2633    2651 
   Size:        598     631     762     213      13     367      49      18

Cuts at:   2651    2796    3138    3436    3555    3644    3681 
   Size:        145     342     298     119      89      37

  Fragments arranged by size: 

                 762     631     598     367     342     298     213     145
                 119      89      49      37      18      13
 
BspEI T'CCGG_A

Cuts at:      0     311    3681 
   Size:        311    3370
 
BspHI T'CATG_A

Cuts at:      0    3368    3681 
   Size:       3368     313
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1070    1601    1712    2187    2723    3053    3681 
   Size:       1070     531     111     475     536     330     628

  Fragments arranged by size: 

                1070     628     536     531     475     330     111
 
BsrBI CCG'CTC

Cuts at:      0     408     414     516     729     933    3681 
   Size:        408       6     102     213     204    2748

  Fragments arranged by size: 

                2748     408     213     204     102       6
 
BsrDI GCAATG_nn'

Cuts at:      0    1325    3681 
   Size:       1325    2356
 
BsrFI r'CCGG_y

Cuts at:      0    2569    3681 
   Size:       2569    1112
 
BssSI C'ACGA_G

Cuts at:      0    1963    3180    3358    3681 
   Size:       1963    1217     178     323

  Fragments arranged by size: 

                1963    1217     323     178
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1062    1593    1620    1704    2193    2715    3681 
   Size:       1062     531      27      84     489     522     966

  Fragments arranged by size: 

                1062     966     531     522     489      84      27
 
BstEII G'GTnAC_C

Cuts at:      0     289    2262    3681 
   Size:        289    1973    1419

  Fragments arranged by size: 

                1973    1419     289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     364    1158    1776    1950    2113    3681 
   Size:        364     794     618     174     163    1568

  Fragments arranged by size: 

                1568     794     618     364     174     163
 
BstYI r'GATC_y

Cuts at:      0    1892    2522    2654    2783    2909    3608    3681 
   Size:       1892     630     132     129     126     699      73

  Fragments arranged by size: 

                1892     699     630     132     129     126      73
 
BtgI C'CryG_G

Cuts at:      0    3512    3644    3681 
   Size:       3512     132      37

  Fragments arranged by size: 

                3512     132      37
 
BtsI GCAGTG_nn'

Cuts at:      0     946    1554    1613    2186    3055    3397    3597    3681 
   Size:        946     608      59     573     869     342     200      84

  Fragments arranged by size: 

                 946     869     608     573     342     200      84      59
            
 
ClaI AT'CG_AT

Cuts at:      0      33    2462    3681 
   Size:         33    2429    1219

  Fragments arranged by size: 

                2429    1219      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2400    2435    2790    2825    3681 
   Size:       2400      35     355      35     856

  Fragments arranged by size: 

                2400     856     355      35      35
 
EaeI y'GGCC_r

Cuts at:      0      95     300    1205    1319    1778    2115    2244    2672 
   Size:         95     205     905     114     459     337     129     428

Cuts at:   2672    3533    3681 
   Size:        861     148

  Fragments arranged by size: 

                 905     861     459     428     337     205     148     129
                 114      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     242     275     371     644    1187    1448    1522    1790 
   Size:        242      33      96     273     543     261      74     268

Cuts at:   1790    2146    3681 
   Size:        356    1535

  Fragments arranged by size: 

                1535     543     356     273     268     261     242      96
                  74      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3439    3681 
   Size:       3439     242
 
EcoICRI GAG'CTC

Cuts at:      0    2631    3136    3681 
   Size:       2631     505     545

  Fragments arranged by size: 

                2631     545     505
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1192    1597    1841    2053    2127    2240    2311    2320 
   Size:       1192     405     244     212      74     113      71       9

Cuts at:   2320    2341    2366    3602    3681 
   Size:         21      25    1236      79

  Fragments arranged by size: 

                1236    1192     405     244     212     113      79      74
                  71      25      21       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1816    3681 
   Size:       1816    1865
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2213    2214    2712    2792    2793    3433    3681 
   Size:       2213       1     498      80       1     640     248

  Fragments arranged by size: 

                2213     640     498     248      80       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2208    3681 
   Size:       2208    1473
 
HaeII r_GCGC'y

Cuts at:      0     681    1128    1971    2025    2618    3532    3681 
   Size:        681     447     843      54     593     914     149

  Fragments arranged by size: 

                 914     843     681     593     447     149      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     337     369     544     576    3600    3632    3681 
   Size:        337      32     175      32    3024      32      49

  Fragments arranged by size: 

                3024     337     175      49      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2133    2442    3681 
   Size:       2133     309    1239

  Fragments arranged by size: 

                2133    1239     309
 
MscI TGG'CCA

Cuts at:      0     302    1321    1780    2246    2674    3681 
   Size:        302    1019     459     466     428    1007

  Fragments arranged by size: 

                1019    1007     466     459     428     302
 
MslI CAynn'nnrTG

Cuts at:      0     362    1712    1774    1937    1948    1984    2260    2644 
   Size:        362    1350      62     163      11      36     276     384

Cuts at:   2644    3154    3286    3681 
   Size:        510     132     395

  Fragments arranged by size: 

                1350     510     395     384     362     276     163     132
                  62      36      11
 
MspA1I CmG'CkG

Cuts at:      0    1017    1098    1692    2343    2556    2850    3165    3403 
   Size:       1017      81     594     651     213     294     315     238

Cuts at:   3403    3546    3681 
   Size:        143     135

  Fragments arranged by size: 

                1017     651     594     315     294     238     213     143
                 135      81
 
NaeI GCC'GGC

Cuts at:      0    2571    3681 
   Size:       2571    1110
 
NcoI C'CATG_G

Cuts at:      0    3512    3644    3681 
   Size:       3512     132      37

  Fragments arranged by size: 

                3512     132      37
 
NgoMIV G'CCGG_C

Cuts at:      0    2569    3681 
   Size:       2569    1112
 
NspI r_CATG'y

Cuts at:      0     112    1146    2760    3681 
   Size:        112    1034    1614     921

  Fragments arranged by size: 

                1614    1034     921     112
 
PasI CC'CwG_GG

Cuts at:      0    3585    3681 
   Size:       3585      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36    1170    2280    2733    3077    3681 
   Size:         36    1134    1110     453     344     604

  Fragments arranged by size: 

                1134    1110     604     453     344      36
 
PfoI T'CCnGG_A

Cuts at:      0    1574    1913    2003    2990    3122    3681 
   Size:       1574     339      90     987     132     559

  Fragments arranged by size: 

                1574     987     559     339     132      90
 
PpuMI rG'GwC_Cy

Cuts at:      0    2712    3681 
   Size:       2712     969
 
PspOMI G'GGCC_C

Cuts at:      0    2213    2580    2792    3432    3681 
   Size:       2213     367     212     640     249

  Fragments arranged by size: 

                2213     640     367     249     212
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3049    3081    3681 
   Size:       3049      32     600

  Fragments arranged by size: 

                3049     600      32
 
PstI C_TGCA'G

Cuts at:      0    2181    2522    2723    2902    3050    3681 
   Size:       2181     341     201     179     148     631

  Fragments arranged by size: 

                2181     631     341     201     179     148
 
PvuI CG_AT'CG

Cuts at:      0     969    3681 
   Size:        969    2712
 
PvuII CAG'CTG

Cuts at:      0    1692    2343    3546    3681 
   Size:       1692     651    1203     135

  Fragments arranged by size: 

                1692    1203     651     135
 
SacI G_AGCT'C

Cuts at:      0    2633    3138    3681 
   Size:       2633     505     543

  Fragments arranged by size: 

                2633     543     505
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1448    3681 
   Size:       1448    2233
 
ScaI AGT'ACT

Cuts at:      0     781    3681 
   Size:        781    2900
 
SfcI C'TryA_G

Cuts at:      0    1494    1935    2177    2518    2719    2898    2952    3046 
   Size:       1494     441     242     341     201     179      54      94

Cuts at:   3046    3444    3681 
   Size:        398     237

  Fragments arranged by size: 

                1494     441     398     341     242     237     201     179
                  94      54
 
SmlI C'TyrA_G

Cuts at:      0     672     749    2692    3142    3250    3681 
   Size:        672      77    1943     450     108     431

  Fragments arranged by size: 

                1943     672     450     431     108      77
 
SphI G_CATG'C

Cuts at:      0    1146    3681 
   Size:       1146    2535
 
SspI AAT'ATT

Cuts at:      0    2418    3681 
   Size:       2418    1263
 
StyI C'CwwG_G

Cuts at:      0    1208    1812    2960    3377    3512    3644    3681 
   Size:       1208     604    1148     417     135     132      37

  Fragments arranged by size: 

                1208    1148     604     417     135     132      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     320    1730    2623    2925    3681 
   Size:        320    1410     893     302     756

  Fragments arranged by size: 

                1410     893     756     320     302
 
TatI w'GTAC_w

Cuts at:      0     779    3681 
   Size:        779    2902
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     838     870    1954    1986    3400    3432    3681 
   Size:        838      32    1084      32    1414      32     249

  Fragments arranged by size: 

                1414    1084     838     249      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3202    3681 
   Size:       3202     479
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1314    2505    3681 
   Size:       1314    1191    1176

  Fragments arranged by size: 

                1314    1191    1176
 
XhoI C'TCGA_G

Cuts at:      0     749    3681 
   Size:        749    2932
 
ZraI GAC'GTC

Cuts at:      0    2429    3681 
   Size:       2429    1252
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI    CspCI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI     PsrI
     PstI     PvuI    PvuII     SacI     SapI     ScaI     SfcI     SmlI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI    BfrBI     BmtI
     BsaI    BsiWI     BsmI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
    BtgZI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     XbaI
     XmaI     XmnI
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