Restriction map for
pFN21ASDA0223
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# NO CUT ENZYMES ( 70 enzymes) #
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AclI AgeI AocI AscI AseI
AsnI Asp700I AvrII BamHI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco81I EcoO65I
EcoRV FbaI FseI HincII HindII
HindIII HpaI MamI MfeI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1948 GGTACC G^GTACC
AccIII 2114 TCCGGA T^CCGGA
AcsI 3400 GAATTC [GA]^AATT[CT]
AflII 397 CTTAAG C^TTAAG
AflIII 2409 ACGCGT A^C[GA][CT]GT
AhdI 974 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3400 GAATTC [GA]^AATT[CT]
Asp718I 1948 GGTACC G^GTACC
AspEI 974 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1551 GGCGCC GGCGC^C
BbrPI 1627 CACGTG CAC^GTG
BcgI 2792 GCATCGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1443 GGATAC GTATCC(6/5)
BfrI 397 CTTAAG C^TTAAG
BsaI 2938 GAGACC GGTCTC(1/5)
BseAI 2114 TCCGGA T^CCGGA
BsiEI 1756 CGGCCG CG[GA][CT]^CG
BspEI 2114 TCCGGA T^CCGGA
BspHI 26 TCATGA T^CATGA
BsrDI 549 CATTGC GCAATG(2/0)
BssHII 1298 GCGCGC G^CGCGC
BstAPI 2718 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1469 AGATCC [GA]^GATC[CT]
EagI 1756 CGGCCG C^GGCCG
Eam1105I 974 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1756 CGGCCG C^GGCCG
Eco52I 1756 CGGCCG C^GGCCG
EcoRI 3400 GAATTC G^AATTC
KasI 1551 GGCGCC G^GCGCC
KpnI 1948 GGTACC GGTAC^C
KspI 442 CCGCGG CCGC^GG
MflI 1469 AGATCC [GA]^GATC[CT]
MluI 2409 ACGCGT A^CGCGT
MroI 2114 TCCGGA T^CCGGA
MslI 1401 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1551 GGCGCC GG^CGCC
NcoI 725 CCATGG C^CATGG
NdeI 1540 CATATG CA^TATG
NspI 998 ACATGC [GA]CATG^[CT]
PaeR7I 714 CTCGAG C^TCGAG
PflFI 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1627 CACGTG CAC^GTG
PmlI 1627 CACGTG CAC^GTG
RcaI 26 TCATGA T^CATGA
SacII 442 CCGCGG CCGC^GG
SapI 1725 GAAGAGC GCTCTTC(1/4)
SbfI 3380 CCTGCAGG CCTGCA^GG
SexAI 1841 ACCAGGT A^CC[AT]GGT
SfoI 1551 GGCGCC GGC^GCC
Sse8387I 3380 CCTGCAGG CCTGCA^GG
Tsp509I 3401 AATT ^AATT
Tth111I 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 714 CTCGAG C^TCGAG
XhoII 1469 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 331 GACGTC GACGT^C
1935 GACGTC
AccI 2161 GTCTAC GT^[AC][GT]AC
3025 GTCTAC
AlwI 1470 GATCC GGATC(4/5)
2744 GGATC
Aor51HI 827 AGCGCT AGC^GCT
1232 AGCGCT
ApaI 446 GGGCCC GGGCC^C
2803 GGGCCC
AviII 1205 TGCGCA TGC^GCA
2369 TGCGCA
BsaAI 1225 TACGTG [CT]AC^GT[GA]
1627 CACGTG
BsaWI 272 ACCGGA [AT]^CCGG[AT]
2114 TCCGGA
EarI 1725 GAAGAG CTCTTC(1/4)
2185 GAAGAG
Eco47III 827 AGCGCT AGC^GCT
1232 AGCGCT
EcoT22I 481 ATGCAT ATGCA^T
1537 ATGCAT
FspI 1205 TGCGCA TGC^GCA
2369 TGCGCA
Ksp632I 1725 GAAGAG CTCTTC(1/4)
2185 GAAGAG
MseI 398 TTAA T^TAA
1919 TTAA
NsiI 481 ATGCAT ATGCA^T
1537 ATGCAT
Tru9I 398 TTAA T^TAA
1919 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI