(Circular) (Six-base) MAPSORT of: pFN21ASDA0223.seq Check: 7833 from: 1 to: 8137 pFN21ASDA0223 8137 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3434 3841 3434 Size: 407 7730 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2358 3962 5947 278 Size: 53 83 186 936 822 1604 1985 2468 Fragments arranged by size: 2468 1985 1604 936 822 186 83 53 AccI GT'mk_AC Cuts at: 1937 4185 5049 5480 1937 Size: 2248 864 431 4594 Fragments arranged by size: 4594 2248 864 431 Acc65I G'GTAC_C Cuts at: 3971 5458 3971 Size: 1487 6650 AclI AA'CG_TT Cuts at: 1563 6264 6637 1563 Size: 4701 373 3063 Fragments arranged by size: 4701 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2647 4008 4294 4625 6200 7584 7852 1632 Size: 1015 1361 286 331 1575 1384 268 1917 Fragments arranged by size: 1917 1575 1384 1361 1015 331 286 268 AfeI AGC'GCT Cuts at: 2852 3257 2852 Size: 405 7732 AflII C'TTAA_G Cuts at: 820 1017 2420 820 Size: 197 1403 6537 Fragments arranged by size: 6537 1403 197 AflIII A'CryG_T Cuts at: 1203 4432 7036 8013 1203 Size: 3229 2604 977 1327 Fragments arranged by size: 3229 2604 1327 977 AgeI A'CCGG_T Cuts at: 6968 6968 Size: 8137 AhdI GACnn_n'nnGTC Cuts at: 3002 6866 3002 Size: 3864 4273 AleI CACnn'nnGTG Cuts at: 1166 3428 1166 Size: 2262 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8137 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 3852 3884 3053 Size: 32 767 32 7306 Fragments arranged by size: 7306 767 32 32 AlwNI CAG_nnn'CTG Cuts at: 3064 3130 3460 3601 4747 5880 7452 3064 Size: 66 330 141 1146 1133 1572 3749 Fragments arranged by size: 3749 1572 1146 1133 330 141 66 ApaI G_GGCC'C Cuts at: 2473 4830 2473 Size: 2357 5780 ApaLI G'TGCA_C Cuts at: 3587 3686 3817 6194 7350 3587 Size: 99 131 2377 1156 4374 Fragments arranged by size: 4374 2377 1156 131 99 ApoI r'AATT_y Cuts at: 1493 5423 5446 5672 5824 7933 1493 Size: 3930 23 226 152 2109 1697 Fragments arranged by size: 3930 2109 1697 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 6691 160 Size: 872 5659 1606 Fragments arranged by size: 5659 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2320 2737 4061 4544 4635 4802 Size: 73 164 213 417 1324 483 91 167 Cuts at: 4802 4967 5091 5268 5462 1870 Size: 165 124 177 194 4545 Fragments arranged by size: 4545 1324 483 417 213 194 177 167 165 164 124 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3459 3492 3459 Size: 33 8104 BamHI G'GATC_C Cuts at: 5467 5834 5467 Size: 367 7770 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 2564 3098 3186 3574 Size: 325 217 627 555 222 534 88 388 Cuts at: 3574 3971 5017 5458 6913 618 Size: 397 1046 441 1455 1842 Fragments arranged by size: 1842 1455 1046 627 555 534 441 397 388 325 222 217 88 BanII G_rGCy'C Cuts at: 1391 2049 2303 2473 3018 3681 3705 3800 4581 Size: 658 254 170 545 663 24 95 781 Cuts at: 4581 4704 4830 5402 5456 1391 Size: 123 126 572 54 4072 Fragments arranged by size: 4072 781 663 658 572 545 254 170 126 123 95 54 24 BbeI G_GCGC'C Cuts at: 3578 3578 Size: 8137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2805 3449 4933 928 Size: 971 906 644 1484 4132 Fragments arranged by size: 4132 1484 971 906 644 BbvCI CC'TCA_GC Cuts at: 2403 5001 5177 2403 Size: 2598 176 5363 Fragments arranged by size: 5363 2598 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4804 4838 6328 6362 1903 Size: 34 2867 34 1490 34 3678 Fragments arranged by size: 3678 2867 1490 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3460 6029 7239 3460 Size: 2569 1210 4358 Fragments arranged by size: 4358 2569 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8137 BfrBI ATG'CAT Cuts at: 2506 3562 2506 Size: 1056 7081 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2243 4628 5238 Size: 107 122 71 631 605 571 2385 610 Cuts at: 5238 6748 136 Size: 1510 1525 Fragments arranged by size: 2385 1525 1510 631 610 605 571 122 107 71 BglII A'GATC_T Cuts at: 8132 8132 Size: 8137 BlpI GC'TnA_GC Cuts at: 5554 5554 Size: 8137 Bme1580I G_kGCm'C Cuts at: 1790 2473 2783 3101 3196 3591 3690 3745 3821 Size: 683 310 318 95 395 99 55 76 Cuts at: 3821 3830 4083 4269 4347 4830 5167 6198 7354 Size: 9 253 186 78 483 337 1031 1156 Cuts at: 7354 1790 Size: 2573 Fragments arranged by size: 2573 1156 1031 683 483 395 337 318 310 253 186 99 95 78 76 55 9 BmgBI CAC'GTC Cuts at: 1318 2389 2839 3709 1318 Size: 1071 450 870 5746 Fragments arranged by size: 5746 1071 870 450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 4077 5405 Size: 416 53 83 399 24 874 1786 1328 Cuts at: 5405 5868 6826 442 Size: 463 958 1753 Fragments arranged by size: 1786 1753 1328 958 874 463 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8137 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 5149 2386 Size: 2763 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2014 2183 2636 2663 2951 2969 3074 3086 Size: 775 169 453 27 288 18 105 12 Cuts at: 3086 3395 3482 4262 5048 5207 5276 6797 1239 Size: 309 87 780 786 159 69 1521 2579 Fragments arranged by size: 2579 1521 786 780 775 453 309 288 169 159 105 87 69 27 18 12 Bpu10I CC'TnA_GC Cuts at: 2225 2403 5001 5177 2225 Size: 178 2598 176 5185 Fragments arranged by size: 5185 2598 178 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2192 2432 2471 4505 6262 7127 7425 2192 Size: 240 39 2034 1757 865 298 2904 Fragments arranged by size: 2904 2034 1757 865 298 240 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 6800 882 Size: 617 3456 1845 2219 Fragments arranged by size: 3456 2219 1845 617 BsaAI yAC'GTr Cuts at: 493 1204 3250 3652 5434 5928 493 Size: 711 2046 402 1782 494 2702 Fragments arranged by size: 2702 2046 1782 711 494 402 BsaBI GATnn'nnATC Cuts at: 8131 8131 Size: 8137 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 2355 2808 3575 Size: 53 83 186 936 654 168 453 767 Cuts at: 3575 3959 4631 5944 6326 6961 275 Size: 384 672 1313 382 635 1451 Fragments arranged by size: 1451 1313 936 767 672 654 635 453 384 382 186 168 83 53 BsaWI w'CCGG_w Cuts at: 2295 4137 6570 6968 7242 7389 7800 2295 Size: 1842 2433 398 274 147 411 2632 Fragments arranged by size: 2632 2433 1842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 2942 2972 4430 Size: 30 165 30 1014 30 495 30 1458 Cuts at: 4430 4460 1178 Size: 30 4855 Fragments arranged by size: 4855 1458 1014 495 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2843 3317 3320 3392 3395 4004 4864 4961 Size: 1657 474 3 72 3 609 860 97 Cuts at: 4961 4964 4973 5141 5261 1186 Size: 3 9 168 120 4062 Fragments arranged by size: 4062 1657 860 609 474 168 120 97 72 9 3 3 3 BseYI C'CCAG_C Cuts at: 2093 2174 2789 5304 7340 7836 2093 Size: 81 615 2515 2036 496 2394 Fragments arranged by size: 2515 2394 2036 615 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3107 3272 5101 1163 Size: 573 1371 165 1829 4199 Fragments arranged by size: 4199 1829 1371 573 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3782 5587 6348 7376 1540 Size: 275 186 1781 1805 761 1028 2301 Fragments arranged by size: 2301 1805 1781 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 3591 3690 3821 4581 4704 6198 6283 7354 Size: 1542 99 131 760 123 1494 85 1071 Cuts at: 7354 2049 Size: 2832 Fragments arranged by size: 2832 1542 1494 1071 760 131 123 99 85 BsmI GAATG_Cn' Cuts at: 5648 5741 5648 Size: 93 8044 BsmBI CGTCTCn'nnnn_ Cuts at: 2540 2639 3715 4238 2540 Size: 99 1076 523 6439 Fragments arranged by size: 6439 1076 523 99 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2303 2473 2783 3018 3101 3196 Size: 399 259 254 170 310 235 83 95 Cuts at: 3196 3591 3681 3690 3705 3745 3800 3821 3830 Size: 395 90 9 15 40 55 21 9 Cuts at: 3830 4083 4269 4347 4581 4704 4830 5167 5402 Size: 253 186 78 234 123 126 337 235 Cuts at: 5402 5456 6198 6283 7354 1391 Size: 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 395 337 310 259 254 253 235 235 234 186 170 126 123 95 90 85 83 78 55 54 40 21 15 9 9 BspEI T'CCGG_A Cuts at: 4137 7800 4137 Size: 3663 4474 BspHI T'CATG_A Cuts at: 2049 6026 7756 2049 Size: 3977 1730 2430 Fragments arranged by size: 3977 2430 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3234 3434 3841 4539 4676 4722 4755 5492 Size: 2390 200 407 698 137 46 33 737 Cuts at: 5492 844 Size: 3489 Fragments arranged by size: 3489 2390 737 698 407 200 137 46 33 BsrBI CCG'CTC Cuts at: 1394 1823 3028 3886 3955 4381 4552 6024 1394 Size: 429 1205 858 69 426 171 1472 3507 Fragments arranged by size: 3507 1472 1205 858 429 426 171 69 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 6632 6806 65 Size: 1183 1323 4061 174 1396 Fragments arranged by size: 4061 1396 1323 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 2561 2810 4069 4131 4641 6781 Size: 110 535 216 249 1259 62 510 2140 Cuts at: 6781 6968 1700 Size: 187 2869 Fragments arranged by size: 2869 2140 1259 535 510 249 216 187 110 62 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8137 BssHII G'CGCG_C Cuts at: 1925 3321 1925 Size: 1396 6741 BssSI C'ACGA_G Cuts at: 2474 3152 3287 4100 4574 6197 7209 2474 Size: 678 135 813 474 1623 1012 3402 Fragments arranged by size: 3402 1623 1012 813 678 474 135 BstAPI GCAn_nnn'nTGC Cuts at: 794 4747 794 Size: 3953 4184 BstBI TT'CG_AA Cuts at: 2007 6941 2007 Size: 4934 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8137 BstXI CCAn_nnnn'nTGG Cuts at: 2896 3355 5167 2896 Size: 459 1812 5866 Fragments arranged by size: 5866 1812 459 BstYI r'GATC_y Cuts at: 1972 3492 5467 5834 6219 6236 7688 7774 7786 Size: 1520 1975 367 385 17 1452 86 12 Cuts at: 7786 8132 1972 Size: 346 1977 Fragments arranged by size: 1977 1975 1520 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2465 2541 2748 3984 4844 5377 513 Size: 552 1400 76 207 1236 860 533 3273 Fragments arranged by size: 3273 1400 1236 860 552 533 207 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7398 Fragments arranged by size: 7398 683 56 BtsI GCAGTG_nn' Cuts at: 2727 2779 2876 4674 5248 5357 5653 6447 6467 Size: 52 97 1798 574 109 296 794 20 Cuts at: 6467 2727 Size: 4397 Fragments arranged by size: 4397 1798 794 574 296 109 97 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8102 DraI TTT'AAA Cuts at: 5441 5441 Size: 8137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7144 809 Size: 507 5828 1802 Fragments arranged by size: 5828 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 3073 3291 Size: 54 1425 50 275 18 1068 175 218 Cuts at: 3291 3342 3411 3432 3534 3779 3881 4074 4251 Size: 51 69 21 102 245 102 193 177 Cuts at: 4251 4300 4590 4841 5584 6473 8102 8 Size: 49 290 251 743 889 1629 43 Fragments arranged by size: 1629 1425 1068 889 743 290 275 251 245 218 193 177 175 102 102 69 54 51 50 49 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3779 5584 1537 Size: 275 1967 1805 4090 Fragments arranged by size: 4090 1967 1805 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3743 4203 6067 1353 Size: 2390 460 1864 3423 Fragments arranged by size: 3423 2390 1864 460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2399 3230 3314 3413 3703 4085 Size: 1064 138 810 831 84 99 290 382 Cuts at: 4085 4553 5087 5401 6724 7098 7244 387 Size: 468 534 314 1323 374 146 1280 Fragments arranged by size: 1323 1280 1064 831 810 534 468 382 374 314 290 146 138 99 84 EcoICRI GAG'CTC Cuts at: 2047 4579 4702 2047 Size: 2532 123 5482 Fragments arranged by size: 5482 2532 123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2014 2183 2636 2647 2663 2951 2969 Size: 393 382 169 453 11 16 288 18 Cuts at: 2969 3074 3086 3395 3482 4008 4262 4294 4625 Size: 105 12 309 87 526 254 32 331 Cuts at: 4625 5048 5207 5276 6200 6797 7584 7852 1239 Size: 423 159 69 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 526 453 423 393 382 331 309 288 268 254 169 159 105 87 69 32 18 16 12 11 EcoNI CCTnn'n_nnAGG Cuts at: 2450 2486 3056 3230 3930 5126 2450 Size: 36 570 174 700 1196 5461 Fragments arranged by size: 5461 1196 700 570 174 36 EcoO109I rG'GnC_Cy Cuts at: 2178 2202 2407 2638 2914 2968 3061 4711 5353 Size: 24 205 231 276 54 93 1650 642 Cuts at: 5353 2178 Size: 4962 Fragments arranged by size: 4962 1650 642 276 231 205 93 54 24 EcoRI G'AATT_C Cuts at: 5423 5446 5824 5423 Size: 23 378 7736 Fragments arranged by size: 7736 378 23 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3298 3772 3298 Size: 474 7663 FspI TGC'GCA Cuts at: 3230 4394 6643 3230 Size: 1164 2249 4724 Fragments arranged by size: 4724 2249 1164 HaeII r_GCGC'y Cuts at: 1678 2854 3259 3485 3578 4332 4391 7284 1678 Size: 1176 405 226 93 754 59 2893 2531 Fragments arranged by size: 2893 2531 1176 754 405 226 93 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2988 3020 3203 3235 3944 Size: 32 135 32 1416 32 183 32 709 Cuts at: 3944 3976 4094 4126 4970 5002 5099 5129 5131 Size: 32 118 32 844 32 97 30 2 Cuts at: 5131 5161 6778 6810 6852 6884 1373 Size: 30 1617 32 42 32 2626 Fragments arranged by size: 2626 1617 1416 844 709 183 135 118 97 42 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 5481 5727 669 Size: 988 281 3543 246 3079 Fragments arranged by size: 3543 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8137 HpaI GTT'AAC Cuts at: 5727 5727 Size: 8137 KasI G'GCGC_C Cuts at: 3574 3574 Size: 8137 KpnI G_GTAC'C Cuts at: 3975 5462 3975 Size: 1487 6650 MfeI C'AATT_G Cuts at: 5736 5736 Size: 8137 MluI A'CGCG_T Cuts at: 4432 8013 4432 Size: 3581 4556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3246 5013 5127 5361 7251 7435 1305 Size: 1941 1767 114 234 1890 184 2007 Fragments arranged by size: 2007 1941 1890 1767 234 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 2900 3293 3413 4253 4592 Size: 54 1425 343 1068 393 120 840 339 Cuts at: 4592 10 Size: 3555 Fragments arranged by size: 3555 1425 1068 840 393 343 339 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 3428 6095 6454 6613 518 Size: 648 2262 2667 359 159 2042 Fragments arranged by size: 2667 2262 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2174 2467 2530 3580 3661 3667 4294 5056 Size: 568 293 63 1050 81 6 627 762 Cuts at: 5056 5238 5254 5553 6230 7378 7623 1606 Size: 182 16 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 1050 762 677 627 568 299 293 245 182 81 63 16 6 NaeI GCC'GGC Cuts at: 2347 2563 2812 4643 2347 Size: 216 249 1831 5841 Fragments arranged by size: 5841 1831 249 216 NarI GG'CG_CC Cuts at: 3575 3575 Size: 8137 NcoI C'CATG_G Cuts at: 513 1065 2748 513 Size: 552 1683 5902 Fragments arranged by size: 5902 1683 552 NdeI CA'TA_TG Cuts at: 387 3564 387 Size: 3177 4960 NgoMIV G'CCGG_C Cuts at: 2345 2561 2810 4641 2345 Size: 216 249 1831 5841 Fragments arranged by size: 5841 1831 249 216 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8137 NotI GC'GGCC_GC Cuts at: 5584 5584 Size: 8137 NsiI A_TGCA'T Cuts at: 2508 3564 2508 Size: 1056 7081 NspI r_CATG'y Cuts at: 1132 1229 3025 5495 7040 1132 Size: 97 1796 2470 1545 2229 Fragments arranged by size: 2470 2229 1796 1545 97 PasI CC'CwG_GG Cuts at: 1348 5167 1348 Size: 3819 4318 PciI A'CATG_T Cuts at: 7036 7036 Size: 8137 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 2749 3082 3723 4010 1108 Size: 1272 369 333 641 287 5235 Fragments arranged by size: 5235 1272 641 369 333 287 PfoI T'CCnGG_A Cuts at: 2030 2273 3495 5184 2030 Size: 243 1222 1689 4983 Fragments arranged by size: 4983 1689 1222 243 PmeI GTTT'AAAC Cuts at: 5441 5441 Size: 8137 PmlI CAC'GTG Cuts at: 3652 3652 Size: 8137 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6206 6238 7719 7751 4718 Size: 32 1456 32 1481 32 5104 Fragments arranged by size: 5104 1481 1456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2178 2202 2914 3061 4711 2178 Size: 24 712 147 1650 5604 Fragments arranged by size: 5604 1650 712 147 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8137 PsiI TTA'TAA Cuts at: 5707 5707 Size: 8137 PspOMI G'GGCC_C Cuts at: 2469 4826 2469 Size: 2357 5780 PspXI vC'TCGA_Gb Cuts at: 2737 2737 Size: 8137 PstI C_TGCA'G Cuts at: 830 2976 2996 3666 4293 4743 5061 5352 5408 Size: 2146 20 670 627 450 318 291 56 Cuts at: 5408 5489 830 Size: 81 3478 Fragments arranged by size: 3478 2146 670 627 450 318 291 81 56 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8137 PvuII CAG'CTG Cuts at: 3661 4294 5254 3661 Size: 633 960 6544 Fragments arranged by size: 6544 960 633 SacI G_AGCT'C Cuts at: 2049 4581 4704 2049 Size: 2532 123 5482 Fragments arranged by size: 5482 2532 123 SacII CC_GC'GG Cuts at: 2468 2468 Size: 8137 SalI G'TCGA_C Cuts at: 1936 5479 1936 Size: 3543 4594 SanDI GG'GwC_CC Cuts at: 2202 2202 Size: 8137 SapI GCTCTTCn'nnn_ Cuts at: 3743 3743 Size: 8137 SbfI CC_TGCA'GG Cuts at: 5408 5489 5408 Size: 81 8056 ScaI AGT'ACT Cuts at: 6385 8097 6385 Size: 1712 6425 SexAI A'CCwGG_T Cuts at: 3864 3864 Size: 8137 SfcI C'TryA_G Cuts at: 826 1045 2846 2972 2992 3662 4289 4739 5057 Size: 219 1801 126 20 670 627 450 318 Cuts at: 5057 5348 5404 5485 6620 7301 7492 826 Size: 291 56 81 1135 681 191 1471 Fragments arranged by size: 1801 1471 1135 681 670 627 450 318 291 219 191 126 81 56 20 SfoI GGC'GCC Cuts at: 3576 3576 Size: 8137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8137 SmaI CCC'GGG Cuts at: 1872 2322 4637 4804 4969 5270 5464 1872 Size: 450 2315 167 165 301 194 4545 Fragments arranged by size: 4545 2315 450 301 194 167 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 2411 2420 2450 2737 4520 Size: 197 926 264 204 9 30 287 1783 Cuts at: 4520 6241 7142 7404 820 Size: 1721 901 262 1553 Fragments arranged by size: 1783 1721 1553 926 901 287 264 262 204 197 30 9 SnaBI TAC'GTA Cuts at: 493 5434 5928 493 Size: 4941 494 2702 Fragments arranged by size: 4941 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8137 SphI G_CATG'C Cuts at: 1132 5495 1132 Size: 4363 3774 SrfI GCCC'GGGC Cuts at: 2322 2322 Size: 8137 SspI AAT'ATT Cuts at: 5 52 6061 5 Size: 47 6009 2081 Fragments arranged by size: 6009 2081 47 StuI AGG'CCT Cuts at: 1517 3182 4454 4493 5132 1517 Size: 1665 1272 39 639 4522 Fragments arranged by size: 4522 1665 1272 639 39 StyI C'CwwG_G Cuts at: 513 1065 2577 2748 2964 3294 3471 3837 4113 Size: 552 1512 171 216 330 177 366 276 Cuts at: 4113 4398 4593 4623 5576 513 Size: 285 195 30 953 3074 Fragments arranged by size: 3074 1512 953 552 366 330 285 276 216 195 177 171 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2474 2681 6164 6181 6334 6519 6964 Size: 1255 894 207 3483 17 153 185 445 Cuts at: 6964 325 Size: 1498 Fragments arranged by size: 3483 1498 1255 894 445 207 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 6383 8095 Size: 274 80 33 51 1443 927 3479 1712 Cuts at: 8095 96 Size: 138 Fragments arranged by size: 3479 1712 1443 927 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4430 4462 8097 8129 4430 Size: 32 3635 32 4438 Fragments arranged by size: 4438 3635 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2357 1316 Size: 1041 7096 XbaI T'CTAG_A Cuts at: 5473 5473 Size: 8137 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3043 3352 3991 5215 3043 Size: 309 639 1224 5965 Fragments arranged by size: 5965 1224 639 309 XhoI C'TCGA_G Cuts at: 1943 2737 1943 Size: 794 7343 XmaI C'CCGG_G Cuts at: 1870 2320 4635 4802 4967 5268 5462 1870 Size: 450 2315 167 165 301 194 4545 Fragments arranged by size: 4545 2315 450 301 194 167 165 XmnI GAAnn'nnTTC Cuts at: 6266 6266 Size: 8137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2356 3960 5945 276 Size: 53 83 186 936 822 1604 1985 2468 Fragments arranged by size: 2468 1985 1604 936 822 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PsrI RsrII SfiI SwaI