(Circular) (Six-base) MAPSORT of: pFN21ASDA0217.seq Check: 1410 from: 1 to: 6943 pFN21ASDA0217 6943 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3922 3922 Size: 6943 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3041 4753 278 Size: 53 83 186 936 1505 1712 2468 Fragments arranged by size: 2468 1712 1505 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4286 1937 Size: 2349 4594 Acc65I G'GTAC_C Cuts at: 2164 4264 2164 Size: 2100 4843 AclI AA'CG_TT Cuts at: 1563 5070 5443 1563 Size: 3507 373 3063 Fragments arranged by size: 3507 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3408 5006 6390 6658 1632 Size: 1776 1598 1384 268 1917 Fragments arranged by size: 1917 1776 1598 1384 268 AfeI AGC'GCT Cuts at: 2095 3029 2095 Size: 934 6009 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6746 AflIII A'CryG_T Cuts at: 1203 2550 5842 6819 1203 Size: 1347 3292 977 1327 Fragments arranged by size: 3292 1347 1327 977 AgeI A'CCGG_T Cuts at: 5774 5774 Size: 6943 AhdI GACnn_n'nnGTC Cuts at: 5672 5672 Size: 6943 AleI CACnn'nnGTG Cuts at: 1166 2600 2639 1166 Size: 1434 39 5470 Fragments arranged by size: 5470 1434 39 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6943 AlwNI CAG_nnn'CTG Cuts at: 2870 4686 6258 2870 Size: 1816 1572 3555 Fragments arranged by size: 3555 1816 1572 ApaLI G'TGCA_C Cuts at: 5000 6156 5000 Size: 1156 5787 ApoI r'AATT_y Cuts at: 1493 2516 2810 2825 3511 4252 4478 4630 6739 Size: 1023 294 15 686 741 226 152 2109 Cuts at: 6739 1493 Size: 1697 Fragments arranged by size: 2109 1697 1023 741 686 294 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 3356 3360 5497 160 Size: 872 2324 4 2137 1606 Fragments arranged by size: 2324 2137 1606 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 AvaI C'yCGr_G Cuts at: 1870 1943 2490 4268 1870 Size: 73 547 1778 4545 Fragments arranged by size: 4545 1778 547 73 BamHI G'GATC_C Cuts at: 4273 4640 4273 Size: 367 6576 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2164 3778 4264 5719 618 Size: 325 217 627 377 1614 486 1455 1842 Fragments arranged by size: 1842 1614 1455 627 486 377 325 217 BanII G_rGCy'C Cuts at: 1391 2218 3774 4089 4110 4138 4262 1391 Size: 827 1556 315 21 28 124 4072 Fragments arranged by size: 4072 1556 827 315 124 28 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 3662 928 Size: 971 591 1172 4209 Fragments arranged by size: 4209 1172 971 591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5134 5168 1903 Size: 34 3197 34 3678 Fragments arranged by size: 3678 3197 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3249 4835 6045 3249 Size: 1586 1210 4147 Fragments arranged by size: 4147 1586 1210 BclI T'GATC_A Cuts at: 1548 2032 3096 1548 Size: 484 1064 5395 Fragments arranged by size: 5395 1064 484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5554 136 Size: 107 122 71 631 605 3882 1525 Fragments arranged by size: 3882 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2666 6938 2666 Size: 4272 2671 BlpI GC'TnA_GC Cuts at: 2630 4360 2630 Size: 1730 5213 Bme1580I G_kGCm'C Cuts at: 1790 5004 6160 1790 Size: 3214 1156 2573 Fragments arranged by size: 3214 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3007 3111 1318 Size: 1689 104 5150 Fragments arranged by size: 5150 1689 104 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4674 5632 442 Size: 416 53 83 399 24 3257 958 1753 Fragments arranged by size: 3257 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3177 3657 3748 4152 5603 1239 Size: 1938 480 91 404 1451 2579 Fragments arranged by size: 2579 1938 1451 480 404 91 Bpu10I CC'TnA_GC Cuts at: 2043 2043 Size: 6943 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4103 5068 5933 6231 4103 Size: 965 865 298 4815 Fragments arranged by size: 4815 965 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3766 4137 5606 882 Size: 617 2267 371 1469 2219 Fragments arranged by size: 2267 2219 1469 617 371 BsaAI yAC'GTr Cuts at: 493 1204 4240 4734 493 Size: 711 3036 494 2702 Fragments arranged by size: 3036 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6937 6937 Size: 6943 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3038 3212 4750 5132 Size: 53 83 186 936 1505 174 1538 382 Cuts at: 5132 5767 275 Size: 635 1451 Fragments arranged by size: 1538 1505 1451 936 635 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 3369 3525 5376 5774 6048 6195 6606 3369 Size: 156 1851 398 274 147 411 3706 Fragments arranged by size: 3706 1851 411 398 274 156 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2691 2721 3530 3553 3560 Size: 30 165 30 1288 30 809 23 7 Cuts at: 3560 3583 1178 Size: 23 4538 Fragments arranged by size: 4538 1288 809 165 30 30 30 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 3207 3234 3554 4221 1186 Size: 1102 919 27 320 667 3908 Fragments arranged by size: 3908 1102 919 667 320 27 BseYI C'CCAG_C Cuts at: 2383 3021 3069 3446 3607 3826 3965 4111 6146 Size: 638 48 377 161 219 139 146 2035 Cuts at: 6146 6642 2383 Size: 496 2684 Fragments arranged by size: 2684 2035 638 496 377 219 161 146 139 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3315 3747 3950 1163 Size: 573 1579 432 203 4156 Fragments arranged by size: 4156 1579 573 432 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3915 4157 4393 5154 6182 1540 Size: 275 186 1914 242 236 761 1028 2301 Fragments arranged by size: 2301 1914 1028 761 275 242 236 186 BsiHKAI G_wGCw'C Cuts at: 4217 5004 5089 6160 4217 Size: 787 85 1071 5000 Fragments arranged by size: 5000 1071 787 85 BsiWI C'GTAC_G Cuts at: 3807 3807 Size: 6943 BsmI GAATG_Cn' Cuts at: 2356 4454 4547 2356 Size: 2098 93 4752 Fragments arranged by size: 4752 2098 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3195 3219 4202 3195 Size: 24 983 5936 Fragments arranged by size: 5936 983 24 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 3774 4089 4110 4138 4217 4262 Size: 399 428 1556 315 21 28 79 45 Cuts at: 4262 5004 5089 6160 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1556 1071 742 428 399 315 85 79 45 28 21 BspEI T'CCGG_A Cuts at: 3369 6606 3369 Size: 3237 3706 BspHI T'CATG_A Cuts at: 4832 6562 4832 Size: 1730 5213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3383 3922 4298 844 Size: 2539 539 376 3489 Fragments arranged by size: 3489 2539 539 376 BsrBI CCG'CTC Cuts at: 1394 1823 4830 1394 Size: 429 3007 3507 Fragments arranged by size: 3507 3007 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2357 4016 5438 5612 65 Size: 1183 1109 1659 1422 174 1396 Fragments arranged by size: 1659 1422 1396 1183 1109 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3644 3781 4156 4167 5587 5774 1700 Size: 110 1834 137 375 11 1420 187 2869 Fragments arranged by size: 2869 1834 1420 375 187 137 110 11 BsrGI T'GTAC_A Cuts at: 96 3060 3087 96 Size: 2964 27 3952 Fragments arranged by size: 3952 2964 27 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6943 BssSI C'ACGA_G Cuts at: 5003 6015 5003 Size: 1012 5931 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 794 Size: 1449 5494 BstBI TT'CG_AA Cuts at: 2007 5747 2007 Size: 3740 3203 BstEII G'GTnAC_C Cuts at: 1184 2333 1184 Size: 1149 5794 BstYI r'GATC_y Cuts at: 1972 2666 4273 4640 5025 5042 6494 6580 6592 Size: 694 1607 367 385 17 1452 86 12 Cuts at: 6592 6938 1972 Size: 346 1977 Fragments arranged by size: 1977 1607 1452 694 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2299 2766 3948 4185 513 Size: 552 1234 467 1182 237 3271 Fragments arranged by size: 3271 1234 1182 552 467 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6204 Fragments arranged by size: 6204 683 56 BtsI GCAGTG_nn' Cuts at: 2248 3342 3906 4459 5253 5273 2248 Size: 1094 564 553 794 20 3918 Fragments arranged by size: 3918 1094 794 564 553 20 ClaI AT'CG_AT Cuts at: 1554 3352 1554 Size: 1798 5145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6908 DraI TTT'AAA Cuts at: 2791 4247 2791 Size: 1456 5487 DraIII CAC_nnn'GTG Cuts at: 3883 3883 Size: 6943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5950 809 Size: 507 4634 1802 Fragments arranged by size: 4634 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2284 4154 4182 Size: 54 1425 50 275 18 454 1870 28 Cuts at: 4182 4390 5279 6908 8 Size: 208 889 1629 43 Fragments arranged by size: 1870 1629 1425 889 454 275 208 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 4154 4390 1537 Size: 275 2342 236 4090 Fragments arranged by size: 4090 2342 275 236 EarI CTCTTCn'nnn_ Cuts at: 1353 4873 1353 Size: 3520 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3872 3889 5530 5904 6050 387 Size: 1064 138 2283 17 1641 374 146 1280 Fragments arranged by size: 2283 1641 1280 1064 374 146 138 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3177 3408 3657 3748 4152 5006 5603 Size: 393 1545 231 249 91 404 854 597 Cuts at: 5603 6390 6658 1239 Size: 787 268 1524 Fragments arranged by size: 1545 1524 854 787 597 404 393 268 249 231 91 EcoNI CCTnn'n_nnAGG Cuts at: 3492 3492 Size: 6943 EcoO109I rG'GnC_Cy Cuts at: 2411 3305 3717 2411 Size: 894 412 5637 Fragments arranged by size: 5637 894 412 EcoRI G'AATT_C Cuts at: 2516 3511 4252 4630 2516 Size: 995 741 378 4829 Fragments arranged by size: 4829 995 741 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3532 3532 Size: 6943 FseI GG_CCGG'CC Cuts at: 4171 4171 Size: 6943 FspI TGC'GCA Cuts at: 5449 5449 Size: 6943 HaeII r_GCGC'y Cuts at: 1678 2097 3031 6090 1678 Size: 419 934 3059 2531 Fragments arranged by size: 3059 2531 934 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2095 2127 2892 Size: 32 135 32 445 32 46 32 765 Cuts at: 2892 2924 3553 3585 5584 5616 5658 5690 1373 Size: 32 629 32 1999 32 42 32 2626 Fragments arranged by size: 2626 1999 765 629 445 135 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4287 4533 669 Size: 988 281 2349 246 3079 Fragments arranged by size: 3079 2349 988 281 246 HindIII A'AGCT_T Cuts at: 748 3598 748 Size: 2850 4093 HpaI GTT'AAC Cuts at: 4533 4533 Size: 6943 KpnI G_GTAC'C Cuts at: 2168 4268 2168 Size: 2100 4843 MfeI C'AATT_G Cuts at: 4542 4542 Size: 6943 MluI A'CGCG_T Cuts at: 6819 6819 Size: 6943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 3596 6057 6241 1305 Size: 1053 1238 2461 184 2007 Fragments arranged by size: 2461 2007 1238 1053 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2286 10 Size: 54 1425 343 454 4667 Fragments arranged by size: 4667 1425 454 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 2600 2639 4901 5260 5419 518 Size: 648 1183 251 39 2262 359 159 2042 Fragments arranged by size: 2262 2042 1183 648 359 251 159 39 MspA1I CmG'CkG Cuts at: 1606 4359 5036 6184 6429 1606 Size: 2753 677 1148 245 2120 Fragments arranged by size: 2753 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3646 3783 4158 4169 3646 Size: 137 375 11 6420 Fragments arranged by size: 6420 375 137 11 NcoI C'CATG_G Cuts at: 513 1065 4185 513 Size: 552 3120 3271 Fragments arranged by size: 3271 3120 552 NdeI CA'TA_TG Cuts at: 387 3430 387 Size: 3043 3900 NgoMIV G'CCGG_C Cuts at: 3644 3781 4156 4167 3644 Size: 137 375 11 6420 Fragments arranged by size: 6420 375 137 11 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6943 NotI GC'GGCC_GC Cuts at: 4390 4390 Size: 6943 NspI r_CATG'y Cuts at: 1132 1229 2554 3284 4301 5846 1132 Size: 97 1325 730 1017 1545 2229 Fragments arranged by size: 2229 1545 1325 1017 730 97 PacI TTA_AT'TAA Cuts at: 3360 3360 Size: 6943 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6943 PciI A'CATG_T Cuts at: 2550 5842 2550 Size: 3292 3651 PflMI CCAn_nnn'nTGG Cuts at: 1108 2987 3167 3635 3883 1108 Size: 1879 180 468 248 4168 Fragments arranged by size: 4168 1879 468 248 180 PfoI T'CCnGG_A Cuts at: 2076 2076 Size: 6943 PmeI GTTT'AAAC Cuts at: 4247 4247 Size: 6943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5012 5044 6525 6557 5012 Size: 32 1481 32 5398 Fragments arranged by size: 5398 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2411 3305 3717 2411 Size: 894 412 5637 Fragments arranged by size: 5637 894 412 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6943 PsiI TTA'TAA Cuts at: 2816 3176 4513 2816 Size: 360 1337 5246 Fragments arranged by size: 5246 1337 360 PstI C_TGCA'G Cuts at: 830 3349 4295 830 Size: 2519 946 3478 Fragments arranged by size: 3478 2519 946 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6943 SalI G'TCGA_C Cuts at: 1936 4285 1936 Size: 2349 4594 SanDI GG'GwC_CC Cuts at: 2411 2411 Size: 6943 SbfI CC_TGCA'GG Cuts at: 4295 4295 Size: 6943 ScaI AGT'ACT Cuts at: 2499 5191 6903 2499 Size: 2692 1712 2539 Fragments arranged by size: 2692 2539 1712 SfcI C'TryA_G Cuts at: 826 1045 3345 3753 4291 5426 6107 6298 826 Size: 219 2300 408 538 1135 681 191 1471 Fragments arranged by size: 2300 1471 1135 681 538 408 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6943 SmaI CCC'GGG Cuts at: 1872 4270 1872 Size: 2398 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4118 5047 5948 6210 820 Size: 197 926 2175 929 901 262 1553 Fragments arranged by size: 2175 1553 929 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4240 4734 493 Size: 3747 494 2702 Fragments arranged by size: 3747 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6943 SphI G_CATG'C Cuts at: 1132 3284 4301 1132 Size: 2152 1017 3774 Fragments arranged by size: 3774 2152 1017 SspI AAT'ATT Cuts at: 5 52 2739 3333 4867 5 Size: 47 2687 594 1534 2081 Fragments arranged by size: 2687 2081 1534 594 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6943 StyI C'CwwG_G Cuts at: 513 1065 3144 3849 4185 4382 513 Size: 552 2079 705 336 197 3074 Fragments arranged by size: 3074 2079 705 552 336 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4970 4987 5140 5325 5770 325 Size: 1255 3390 17 153 185 445 1498 Fragments arranged by size: 3390 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2497 3060 3087 Size: 274 80 33 51 1443 520 563 27 Cuts at: 3087 5189 6901 96 Size: 2102 1712 138 Fragments arranged by size: 2102 1712 1443 563 520 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3709 3741 6903 6935 3709 Size: 32 3162 32 3717 Fragments arranged by size: 3717 3162 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3112 3565 1316 Size: 1796 453 4694 Fragments arranged by size: 4694 1796 453 XbaI T'CTAG_A Cuts at: 4279 4279 Size: 6943 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2297 2297 Size: 6943 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6943 XmaI C'CCGG_G Cuts at: 1870 4268 1870 Size: 2398 4545 XmnI GAAnn'nnTTC Cuts at: 5072 5072 Size: 6943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3039 4751 276 Size: 53 83 186 936 1505 1712 2468 Fragments arranged by size: 2468 1712 1505 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstXI BstZ17I Bsu36I EcoICRI EcoRV FspAI KasI NarI NruI NsiI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI