(Circular) (Six-base) MAPSORT of: pFN21ASDA0214.seq Check: 3187 from: 1 to: 7000 pFN21ASDA0214 7000 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3298 3298 Size: 7000 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2256 4137 4810 278 Size: 53 83 186 936 720 1881 673 2468 Fragments arranged by size: 2468 1881 936 720 673 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 4343 1937 Size: 1377 1029 4594 Fragments arranged by size: 4594 1377 1029 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3006 4187 5063 6447 6715 1632 Size: 983 391 1181 876 1384 268 1917 Fragments arranged by size: 1917 1384 1181 983 876 391 268 AflII C'TTAA_G Cuts at: 820 1017 2108 820 Size: 197 1091 5712 Fragments arranged by size: 5712 1091 197 AflIII A'CryG_T Cuts at: 1203 2199 3453 4260 5899 6876 1203 Size: 996 1254 807 1639 977 1327 Fragments arranged by size: 1639 1327 1254 996 977 807 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 3580 1166 Size: 2414 4586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2559 4179 1184 Size: 1375 1620 4005 Fragments arranged by size: 4005 1620 1375 AlwNI CAG_nnn'CTG Cuts at: 2248 4096 4279 4743 6315 2248 Size: 1848 183 464 1572 2933 Fragments arranged by size: 2933 1848 1572 464 183 ApaI G_GGCC'C Cuts at: 3698 3698 Size: 7000 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 3040 3227 4309 4535 4687 6796 1493 Size: 1547 187 1082 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 1082 226 187 152 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2822 3171 4140 4325 1870 Size: 73 879 349 969 185 4545 Fragments arranged by size: 4545 969 879 349 185 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4259 4292 4259 Size: 33 6967 BamHI G'GATC_C Cuts at: 4330 4697 4330 Size: 367 6633 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3339 4321 5776 618 Size: 325 217 627 1552 982 1455 1842 Fragments arranged by size: 1842 1552 1455 982 627 325 217 BanII G_rGCy'C Cuts at: 1391 2474 2823 3252 3698 3800 3942 4257 4319 Size: 1083 349 429 446 102 142 315 62 Cuts at: 4319 1391 Size: 4072 Fragments arranged by size: 4072 1083 446 429 349 315 142 102 62 BbeI G_GCGC'C Cuts at: 3343 3343 Size: 7000 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 3092 3645 928 Size: 971 303 890 553 4283 Fragments arranged by size: 4283 971 890 553 303 BbvCI CC'TCA_GC Cuts at: 2088 2088 Size: 7000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 5191 5225 1903 Size: 34 98 34 3122 34 3678 Fragments arranged by size: 3678 3122 98 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4892 6102 4892 Size: 1210 5790 BclI T'GATC_A Cuts at: 1548 2358 1548 Size: 810 6190 BfrBI ATG'CAT Cuts at: 2099 2807 2099 Size: 708 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5611 136 Size: 107 122 71 631 605 3939 1525 Fragments arranged by size: 3939 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6995 6995 Size: 7000 BlpI GC'TnA_GC Cuts at: 4417 4417 Size: 7000 Bme1580I G_kGCm'C Cuts at: 1790 2438 2588 3698 5061 6217 1790 Size: 648 150 1110 1363 1156 2573 Fragments arranged by size: 2573 1363 1156 1110 648 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3067 3452 4731 Size: 416 53 83 399 24 1650 385 1279 Cuts at: 4731 5689 442 Size: 958 1753 Fragments arranged by size: 1753 1650 1279 958 416 399 385 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2782 3085 2782 Size: 303 6697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3266 3372 4172 5660 1239 Size: 2027 106 800 1488 2579 Fragments arranged by size: 2579 2027 1488 800 106 Bpu10I CC'TnA_GC Cuts at: 2088 2940 4223 2088 Size: 852 1283 4865 Fragments arranged by size: 4865 1283 852 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3243 3383 5125 5990 6288 3243 Size: 140 1742 865 298 3955 Fragments arranged by size: 3955 1742 865 298 140 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5663 882 Size: 617 4164 2219 Fragments arranged by size: 4164 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2202 4297 4791 493 Size: 711 998 2095 494 2702 Fragments arranged by size: 2702 2095 998 711 494 BsaBI GATnn'nnATC Cuts at: 6994 6994 Size: 7000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2253 3340 3761 4134 Size: 53 83 186 936 720 1087 421 373 Cuts at: 4134 4807 5189 5824 275 Size: 673 382 635 1451 Fragments arranged by size: 1451 1087 936 720 673 635 421 382 373 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3452 3482 3879 3909 4049 Size: 30 165 30 2049 30 397 30 140 Cuts at: 4049 4079 1178 Size: 30 4099 Fragments arranged by size: 4099 2049 397 165 140 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2500 2849 3064 3074 3344 3827 3903 3941 Size: 1314 349 215 10 270 483 76 38 Cuts at: 3941 1186 Size: 4245 Fragments arranged by size: 4245 1314 483 349 270 215 76 38 10 BseYI C'CCAG_C Cuts at: 2158 2799 2887 3773 4286 6203 6699 2158 Size: 641 88 886 513 1917 496 2459 Fragments arranged by size: 2459 1917 886 641 513 496 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3143 3404 1163 Size: 573 1407 261 4759 Fragments arranged by size: 4759 1407 573 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4450 5211 6239 1540 Size: 275 186 2449 761 1028 2301 Fragments arranged by size: 2449 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2354 2952 3252 4257 5061 5146 6217 2354 Size: 598 300 1005 804 85 1071 3137 Fragments arranged by size: 3137 1071 1005 804 598 300 85 BsmI GAATG_Cn' Cuts at: 2101 2215 3873 4511 4604 2101 Size: 114 1658 638 93 4497 Fragments arranged by size: 4497 1658 638 114 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2768 2768 Size: 7000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 2438 2474 2588 2823 2952 3252 Size: 399 564 84 36 114 235 129 300 Cuts at: 3252 3698 3800 3942 4257 4319 5061 5146 6217 Size: 446 102 142 315 62 742 85 1071 Cuts at: 6217 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 564 446 399 315 300 235 142 129 114 102 85 84 62 36 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3172 3298 4287 4355 844 Size: 2328 126 989 68 3489 Fragments arranged by size: 3489 2328 989 126 68 BsrBI CCG'CTC Cuts at: 1394 1823 3211 4887 1394 Size: 429 1388 1676 3507 Fragments arranged by size: 3507 1676 1388 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2347 3327 3921 5495 5669 65 Size: 1183 1099 980 594 1574 174 1396 Fragments arranged by size: 1574 1396 1183 1099 980 594 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2546 3836 4235 5644 5831 1700 Size: 110 736 1290 399 1409 187 2869 Fragments arranged by size: 2869 1409 1290 736 399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 5060 6072 5060 Size: 1012 5988 BstAPI GCAn_nnn'nTGC Cuts at: 794 3499 794 Size: 2705 4295 BstBI TT'CG_AA Cuts at: 2007 5804 2007 Size: 3797 3203 BstEII G'GTnAC_C Cuts at: 1184 2261 3832 1184 Size: 1077 1571 4352 Fragments arranged by size: 4352 1571 1077 BstXI CCAn_nnnn'nTGG Cuts at: 2647 2875 2647 Size: 228 6772 BstYI r'GATC_y Cuts at: 1972 4330 4697 5082 5099 6551 6637 6649 6995 Size: 2358 367 385 17 1452 86 12 346 Cuts at: 6995 1972 Size: 1977 Fragments arranged by size: 2358 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3471 3828 3867 513 Size: 552 2406 357 39 3646 Fragments arranged by size: 3646 2406 552 357 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 2121 3087 3785 3912 4516 5310 5330 2121 Size: 966 698 127 604 794 20 3791 Fragments arranged by size: 3791 966 794 698 604 127 20 ClaI AT'CG_AT Cuts at: 1554 2902 1554 Size: 1348 5652 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2173 2208 529 Size: 35 1609 35 5321 Fragments arranged by size: 5321 1609 35 35 DraI TTT'AAA Cuts at: 4304 4304 Size: 7000 DraIII CAC_nnn'GTG Cuts at: 2752 2752 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3569 6007 809 Size: 507 2253 2438 1802 Fragments arranged by size: 2438 2253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2401 2443 Size: 54 1425 50 275 18 499 72 42 Cuts at: 2443 2700 2771 3324 3474 4447 5336 6965 8 Size: 257 71 553 150 973 889 1629 43 Fragments arranged by size: 1629 1425 973 889 553 499 275 257 150 72 71 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2342 3201 4930 1353 Size: 686 303 859 1729 3423 Fragments arranged by size: 3423 1729 859 686 303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5587 5961 6107 387 Size: 1064 138 3998 374 146 1280 Fragments arranged by size: 3998 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3250 4255 3250 Size: 1005 5995 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 3006 3266 3372 4172 4187 5063 Size: 393 983 391 260 106 800 15 876 Cuts at: 5063 5660 6447 6715 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 983 876 800 787 597 393 391 268 260 106 15 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2525 2988 4154 2465 Size: 60 463 1166 5311 Fragments arranged by size: 5311 1166 463 60 EcoO109I rG'GnC_Cy Cuts at: 2163 3695 3861 3999 4104 2163 Size: 1532 166 138 105 5059 Fragments arranged by size: 5059 1532 166 138 105 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 7000 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 2184 3000 3343 4012 6147 1678 Size: 506 816 343 669 2135 2531 Fragments arranged by size: 2531 2135 816 669 506 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2746 2778 3452 3484 4096 Size: 32 135 32 1174 32 674 32 612 Cuts at: 4096 4128 5641 5673 5715 5747 1373 Size: 32 1513 32 42 32 2626 Fragments arranged by size: 2626 1513 1174 674 612 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 4132 4344 4590 669 Size: 988 281 1377 817 212 246 3079 Fragments arranged by size: 3079 1377 988 817 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KasI G'GCGC_C Cuts at: 3339 3339 Size: 7000 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 2413 4599 2413 Size: 2186 4814 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3865 4092 4224 6114 6298 1305 Size: 2560 227 132 1890 184 2007 Fragments arranged by size: 2560 2007 1890 227 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 2403 2445 2702 10 Size: 54 1425 343 571 42 257 4308 Fragments arranged by size: 4308 1425 571 343 257 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2720 3580 3677 4958 5317 5476 Size: 648 1149 405 860 97 1281 359 159 Cuts at: 5476 518 Size: 2042 Fragments arranged by size: 2042 1281 1149 860 648 405 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2158 2509 2655 2799 3244 3465 4416 5093 Size: 552 351 146 144 445 221 951 677 Cuts at: 5093 6241 6486 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 552 445 351 245 221 146 144 NaeI GCC'GGC Cuts at: 2548 2548 Size: 7000 NarI GG'CG_CC Cuts at: 3340 3340 Size: 7000 NcoI C'CATG_G Cuts at: 513 1065 3828 3867 513 Size: 552 2763 39 3646 Fragments arranged by size: 3646 2763 552 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NgoMIV G'CCGG_C Cuts at: 2546 2546 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NsiI A_TGCA'T Cuts at: 2101 2809 2101 Size: 708 6292 NspI r_CATG'y Cuts at: 1132 1229 2983 3457 3568 3781 4028 4264 4358 Size: 97 1754 474 111 213 247 236 94 Cuts at: 4358 5903 1132 Size: 1545 2229 Fragments arranged by size: 2229 1754 1545 474 247 236 213 111 97 94 PasI CC'CwG_GG Cuts at: 1348 2974 1348 Size: 1626 5374 PciI A'CATG_T Cuts at: 3453 4260 5899 3453 Size: 807 1639 4554 Fragments arranged by size: 4554 1639 807 PflMI CCAn_nnn'nTGG Cuts at: 1108 2373 4018 1108 Size: 1265 1645 4090 Fragments arranged by size: 4090 1645 1265 PfoI T'CCnGG_A Cuts at: 2172 3633 3857 2172 Size: 1461 224 5315 Fragments arranged by size: 5315 1461 224 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3861 4104 3861 Size: 243 6757 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 3408 4570 3408 Size: 1162 5838 PspOMI G'GGCC_C Cuts at: 3694 3694 Size: 7000 PstI C_TGCA'G Cuts at: 830 3082 3495 4044 4076 4284 4352 830 Size: 2252 413 549 32 208 68 3478 Fragments arranged by size: 3478 2252 549 413 208 68 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 2158 2509 2655 3244 2158 Size: 351 146 589 5914 Fragments arranged by size: 5914 589 351 146 RsrII CG'GwC_CG Cuts at: 3109 3109 Size: 7000 SacI G_AGCT'C Cuts at: 3252 4257 3252 Size: 1005 5995 SalI G'TCGA_C Cuts at: 1936 3313 4342 1936 Size: 1377 1029 4594 Fragments arranged by size: 4594 1377 1029 SapI GCTCTTCn'nnn_ Cuts at: 2039 2342 3201 2039 Size: 303 859 5838 Fragments arranged by size: 5838 859 303 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 5248 6960 5248 Size: 1712 5288 SexAI A'CCwGG_T Cuts at: 3735 3735 Size: 7000 SfcI C'TryA_G Cuts at: 826 1045 3078 3491 4040 4072 4280 4348 5483 Size: 219 2033 413 549 32 208 68 1135 Cuts at: 5483 6164 6355 826 Size: 681 191 1471 Fragments arranged by size: 2033 1471 1135 681 549 413 219 208 191 68 32 SfoI GGC'GCC Cuts at: 3341 3341 Size: 7000 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 4142 4327 1872 Size: 2270 185 4545 Fragments arranged by size: 4545 2270 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 3258 3398 5104 6005 6267 Size: 197 926 165 1150 140 1706 901 262 Cuts at: 6267 820 Size: 1553 Fragments arranged by size: 1706 1553 1150 926 901 262 197 165 140 SnaBI TAC'GTA Cuts at: 493 4297 4791 493 Size: 3804 494 2702 Fragments arranged by size: 3804 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 2983 3781 4028 4358 1132 Size: 1851 798 247 330 3774 Fragments arranged by size: 3774 1851 798 330 247 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 2453 1517 Size: 936 6064 StyI C'CwwG_G Cuts at: 513 1065 3828 3867 3984 3990 4439 513 Size: 552 2763 39 117 6 449 3074 Fragments arranged by size: 3074 2763 552 449 117 39 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3234 3821 5027 5044 5197 5382 5827 Size: 1255 1654 587 1206 17 153 185 445 Cuts at: 5827 325 Size: 1498 Fragments arranged by size: 1654 1498 1255 1206 587 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2826 3349 5246 Size: 274 80 33 51 1443 849 523 1897 Cuts at: 5246 6958 96 Size: 1712 138 Fragments arranged by size: 1897 1712 1443 849 523 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3879 3911 6960 6992 3879 Size: 32 3049 32 3887 Fragments arranged by size: 3887 3049 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3444 3737 1316 Size: 2128 293 4579 Fragments arranged by size: 4579 2128 293 XbaI T'CTAG_A Cuts at: 4336 4336 Size: 7000 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 3865 2875 Size: 990 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 4140 4325 1870 Size: 2270 185 4545 Fragments arranged by size: 4545 2270 185 XmnI GAAnn'nnTTC Cuts at: 2244 3012 3686 5129 2244 Size: 768 674 1443 4115 Fragments arranged by size: 4115 1443 768 674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2254 4135 4808 276 Size: 53 83 186 936 720 1881 673 2468 Fragments arranged by size: 2468 1881 936 720 673 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI SacII SanDI SfiI SrfI SwaI