(Circular) (Six-base) MAPSORT of: pFN21ASDA0212.seq Check: 4760 from: 1 to: 6700 pFN21ASDA0212 6700 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3349 3349 Size: 6700 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2974 4043 1937 Size: 1037 1069 4594 Fragments arranged by size: 4594 1069 1037 Acc65I G'GTAC_C Cuts at: 4021 4021 Size: 6700 AclI AA'CG_TT Cuts at: 1563 4827 5200 1563 Size: 3264 373 3063 Fragments arranged by size: 3264 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3843 4763 6147 6415 1632 Size: 2211 920 1384 268 1917 Fragments arranged by size: 2211 1917 1384 920 268 AfeI AGC'GCT Cuts at: 2037 2129 2372 2037 Size: 92 243 6365 Fragments arranged by size: 6365 243 92 AflII C'TTAA_G Cuts at: 820 1017 3229 820 Size: 197 2212 4291 Fragments arranged by size: 4291 2212 197 AflIII A'CryG_T Cuts at: 1203 3294 3747 5599 6576 1203 Size: 2091 453 1852 977 1327 Fragments arranged by size: 2091 1852 1327 977 453 AgeI A'CCGG_T Cuts at: 5531 5531 Size: 6700 AhdI GACnn_n'nnGTC Cuts at: 5429 5429 Size: 6700 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2418 3977 1184 Size: 1234 1559 3907 Fragments arranged by size: 3907 1559 1234 AlwNI CAG_nnn'CTG Cuts at: 2158 3364 3583 4443 6015 2158 Size: 1206 219 860 1572 2843 Fragments arranged by size: 2843 1572 1206 860 219 ApaI G_GGCC'C Cuts at: 2109 2199 2517 2109 Size: 90 318 6292 Fragments arranged by size: 6292 318 90 ApaLI G'TGCA_C Cuts at: 3857 4757 5913 3857 Size: 900 1156 4644 Fragments arranged by size: 4644 1156 900 ApoI r'AATT_y Cuts at: 1493 2614 2957 3812 4009 4235 4387 6496 1493 Size: 1121 343 855 197 226 152 2109 1697 Fragments arranged by size: 2109 1697 1121 855 343 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 5254 160 Size: 872 4222 1606 Fragments arranged by size: 4222 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 2191 2877 4025 1870 Size: 73 248 686 1148 4545 Fragments arranged by size: 4545 1148 686 248 73 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2381 3119 3431 4021 5476 Size: 325 217 627 594 738 312 590 1455 Cuts at: 5476 618 Size: 1842 Fragments arranged by size: 1842 1455 738 627 594 590 325 312 217 BanII G_rGCy'C Cuts at: 1391 2109 2168 2199 2270 2487 2517 4019 1391 Size: 718 59 31 71 217 30 1502 4072 Fragments arranged by size: 4072 1502 718 217 71 59 31 30 BbeI G_GCGC'C Cuts at: 2385 2385 Size: 6700 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 3189 3657 3720 928 Size: 971 187 1103 468 63 3908 Fragments arranged by size: 3908 1103 971 468 187 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4891 4925 1903 Size: 34 2954 34 3678 Fragments arranged by size: 3678 2954 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2230 2587 4592 5802 2230 Size: 357 2005 1210 3128 Fragments arranged by size: 3128 2005 1210 357 BclI T'GATC_A Cuts at: 1548 2649 1548 Size: 1101 5599 BfrBI ATG'CAT Cuts at: 2588 2588 Size: 6700 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 2233 5311 Size: 107 122 71 631 605 500 61 3078 Cuts at: 5311 136 Size: 1525 Fragments arranged by size: 3078 1525 631 605 500 122 107 71 61 BglII A'GATC_T Cuts at: 6695 6695 Size: 6700 BlpI GC'TnA_GC Cuts at: 2138 4117 2138 Size: 1979 4721 Bme1580I G_kGCm'C Cuts at: 1790 2109 2199 2407 2517 3436 3861 4761 5917 Size: 319 90 208 110 919 425 900 1156 Cuts at: 5917 1790 Size: 2573 Fragments arranged by size: 2573 1156 919 900 425 319 208 110 90 BmgBI CAC'GTC Cuts at: 1318 3631 1318 Size: 2313 4387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 3103 4431 Size: 416 53 83 399 24 912 774 1328 Cuts at: 4431 5389 442 Size: 958 1753 Fragments arranged by size: 1753 1328 958 912 774 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3880 5360 1239 Size: 2641 1480 2579 Fragments arranged by size: 2641 2579 1480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4825 5690 5988 4825 Size: 865 298 5537 Fragments arranged by size: 5537 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3261 3487 3674 5363 882 Size: 617 1762 226 187 1689 2219 Fragments arranged by size: 2219 1762 1689 617 226 187 BsaAI yAC'GTr Cuts at: 493 1204 2329 3997 4491 493 Size: 711 1125 1668 494 2702 Fragments arranged by size: 2702 1668 1125 711 494 BsaBI GATnn'nnATC Cuts at: 3786 6694 3786 Size: 2908 3792 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2287 2382 4507 4889 Size: 53 83 186 936 754 95 2125 382 Cuts at: 4889 5524 275 Size: 635 1451 Fragments arranged by size: 2125 1451 936 754 635 382 186 95 83 53 BsaWI w'CCGG_w Cuts at: 3039 3654 5133 5531 5805 5952 6363 3039 Size: 615 1479 398 274 147 411 3376 Fragments arranged by size: 3376 1479 615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3884 3914 1178 Size: 30 165 30 2481 30 3964 Fragments arranged by size: 3964 2481 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2176 2825 3512 3943 1186 Size: 862 128 649 687 431 3943 Fragments arranged by size: 3943 862 687 649 431 128 BseYI C'CCAG_C Cuts at: 2101 2108 2311 2332 5903 6399 2101 Size: 7 203 21 3571 496 2402 Fragments arranged by size: 3571 2402 496 203 21 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6127 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2341 2509 2521 4150 4911 5939 Size: 275 186 340 168 12 1629 761 1028 Cuts at: 5939 1540 Size: 2301 Fragments arranged by size: 2301 1629 1028 761 340 275 186 168 12 BsiHKAI G_wGCw'C Cuts at: 2058 2487 3861 4761 4846 5917 2058 Size: 429 1374 900 85 1071 2841 Fragments arranged by size: 2841 1374 1071 900 429 85 BsmI GAATG_Cn' Cuts at: 4211 4304 4211 Size: 93 6607 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2109 2168 2199 2270 2407 2487 Size: 399 268 51 59 31 71 137 80 Cuts at: 2487 2517 3436 3861 4019 4761 4846 5917 1391 Size: 30 919 425 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 425 399 268 158 137 85 80 71 59 51 31 30 BspEI T'CCGG_A Cuts at: 3039 6363 3039 Size: 3324 3376 BspHI T'CATG_A Cuts at: 3874 4589 6319 3874 Size: 715 1730 4255 Fragments arranged by size: 4255 1730 715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3349 4055 844 Size: 2505 706 3489 Fragments arranged by size: 3489 2505 706 BsrBI CCG'CTC Cuts at: 1394 1823 2137 4587 1394 Size: 429 314 2450 3507 Fragments arranged by size: 3507 2450 429 314 BsrDI GCAATG_nn' Cuts at: 65 1248 2033 3052 3073 3259 5195 5369 65 Size: 1183 785 1019 21 186 1936 174 1396 Fragments arranged by size: 1936 1396 1183 1019 785 186 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 2939 5344 5531 1700 Size: 110 395 734 2405 187 2869 Fragments arranged by size: 2869 2405 734 395 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 4760 5772 4760 Size: 1012 5688 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6700 BstBI TT'CG_AA Cuts at: 2007 5504 2007 Size: 3497 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstXI CCAn_nnnn'nTGG Cuts at: 2319 3098 3983 2319 Size: 779 885 5036 Fragments arranged by size: 5036 885 779 BstYI r'GATC_y Cuts at: 1972 4030 4397 4782 4799 6251 6337 6349 6695 Size: 2058 367 385 17 1452 86 12 346 Cuts at: 6695 1972 Size: 1977 Fragments arranged by size: 2058 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2074 2199 2282 2347 2503 2509 2521 Size: 552 1009 125 83 65 156 6 12 Cuts at: 2521 3803 513 Size: 1282 3410 Fragments arranged by size: 3410 1282 1009 552 156 125 83 65 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5961 Fragments arranged by size: 5961 683 56 BtsI GCAGTG_nn' Cuts at: 2497 3024 4216 5010 5030 2497 Size: 527 1192 794 20 4167 Fragments arranged by size: 4167 1192 794 527 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6665 DraI TTT'AAA Cuts at: 4004 4004 Size: 6700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5707 809 Size: 507 4391 1802 Fragments arranged by size: 4391 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2338 2506 3006 Size: 54 1425 50 275 18 508 168 500 Cuts at: 3006 4147 5036 6665 8 Size: 1141 889 1629 43 Fragments arranged by size: 1629 1425 1141 889 508 500 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2338 2506 4147 1537 Size: 275 526 168 1641 4090 Fragments arranged by size: 4090 1641 526 275 168 EarI CTCTTCn'nnn_ Cuts at: 1353 3229 3485 3819 4630 1353 Size: 1876 256 334 811 3423 Fragments arranged by size: 3423 1876 811 334 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5287 5661 5807 387 Size: 1064 138 3698 374 146 1280 Fragments arranged by size: 3698 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2485 2485 Size: 6700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3843 3880 4763 5360 6147 6415 1239 Size: 393 2211 37 883 597 787 268 1524 Fragments arranged by size: 2211 1524 883 787 597 393 268 37 EcoNI CCTnn'n_nnAGG Cuts at: 3527 3527 Size: 6700 EcoO109I rG'GnC_Cy Cuts at: 2105 2195 2527 2710 3466 2105 Size: 90 332 183 756 5339 Fragments arranged by size: 5339 756 332 183 90 EcoRI G'AATT_C Cuts at: 3812 4009 4387 3812 Size: 197 378 6125 Fragments arranged by size: 6125 378 197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2779 3562 3832 2779 Size: 783 270 5647 Fragments arranged by size: 5647 783 270 FseI GG_CCGG'CC Cuts at: 2209 2209 Size: 6700 FspI TGC'GCA Cuts at: 5206 5206 Size: 6700 HaeII r_GCGC'y Cuts at: 1678 2039 2131 2374 2385 5847 1678 Size: 361 92 243 11 3462 2531 Fragments arranged by size: 3462 2531 361 243 92 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2857 2889 2956 Size: 32 135 32 453 32 800 32 67 Cuts at: 2956 2988 3316 3348 5341 5373 5415 5447 1373 Size: 32 328 32 1993 32 42 32 2626 Fragments arranged by size: 2626 1993 800 453 328 135 67 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2975 3286 4044 4290 669 Size: 988 281 1037 311 758 246 3079 Fragments arranged by size: 3079 1037 988 758 311 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6700 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KasI G'GCGC_C Cuts at: 2381 2381 Size: 6700 KpnI G_GTAC'C Cuts at: 4025 4025 Size: 6700 MfeI C'AATT_G Cuts at: 4299 4299 Size: 6700 MluI A'CGCG_T Cuts at: 6576 6576 Size: 6700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3079 3927 5814 5998 1305 Size: 1774 848 1887 184 2007 Fragments arranged by size: 2007 1887 1774 848 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 10 Size: 54 1425 343 1176 3702 Fragments arranged by size: 3702 1425 1176 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2998 3959 4658 5017 5176 518 Size: 648 1832 961 699 359 159 2042 Fragments arranged by size: 2042 1832 961 699 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2284 2349 2379 2398 2505 2523 2933 3310 Size: 678 65 30 19 107 18 410 377 Cuts at: 3310 3460 4116 4793 5941 6186 1606 Size: 150 656 677 1148 245 2120 Fragments arranged by size: 2120 1148 678 677 656 410 377 245 150 107 65 30 19 18 NaeI GCC'GGC Cuts at: 2207 2207 Size: 6700 NarI GG'CG_CC Cuts at: 2382 2382 Size: 6700 NcoI C'CATG_G Cuts at: 513 1065 2074 3803 513 Size: 552 1009 1729 3410 Fragments arranged by size: 3410 1729 1009 552 NdeI CA'TA_TG Cuts at: 387 3416 387 Size: 3029 3671 NgoMIV G'CCGG_C Cuts at: 2205 2205 Size: 6700 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NsiI A_TGCA'T Cuts at: 2590 2590 Size: 6700 NspI r_CATG'y Cuts at: 1132 1229 2434 2928 3298 3751 3964 4058 5603 Size: 97 1205 494 370 453 213 94 1545 Cuts at: 5603 1132 Size: 2229 Fragments arranged by size: 2229 1545 1205 494 453 370 213 97 94 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6700 PciI A'CATG_T Cuts at: 3294 3747 5599 3294 Size: 453 1852 4395 Fragments arranged by size: 4395 1852 453 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 2821 1108 Size: 920 793 4987 Fragments arranged by size: 4987 920 793 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4769 4801 6282 6314 4769 Size: 32 1481 32 5155 Fragments arranged by size: 5155 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2710 2527 Size: 183 6517 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6700 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PspOMI G'GGCC_C Cuts at: 2105 2195 2513 2105 Size: 90 318 6292 Fragments arranged by size: 6292 318 90 PstI C_TGCA'G Cuts at: 830 2241 3339 3360 3465 3579 4052 830 Size: 1411 1098 21 105 114 473 3478 Fragments arranged by size: 3478 1411 1098 473 114 105 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 2398 3310 3460 2398 Size: 912 150 5638 Fragments arranged by size: 5638 912 150 SacI G_AGCT'C Cuts at: 2487 2487 Size: 6700 SacII CC_GC'GG Cuts at: 2285 2350 2506 2524 2285 Size: 65 156 18 6461 Fragments arranged by size: 6461 156 65 18 SalI G'TCGA_C Cuts at: 1936 2973 4042 1936 Size: 1037 1069 4594 Fragments arranged by size: 4594 1069 1037 SanDI GG'GwC_CC Cuts at: 2527 2527 Size: 6700 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 4948 6660 4948 Size: 1712 4988 SfcI C'TryA_G Cuts at: 826 1045 2237 2374 3335 3356 3461 3575 4048 Size: 219 1192 137 961 21 105 114 473 Cuts at: 4048 5183 5864 6055 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 961 681 473 219 191 137 114 105 21 SfoI GGC'GCC Cuts at: 2383 2383 Size: 6700 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6700 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3229 4804 5705 5967 820 Size: 197 926 1286 1575 901 262 1553 Fragments arranged by size: 1575 1553 1286 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3997 4491 493 Size: 3504 494 2702 Fragments arranged by size: 3504 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6700 SphI G_CATG'C Cuts at: 1132 4058 1132 Size: 2926 3774 SspI AAT'ATT Cuts at: 5 52 3692 4624 5 Size: 47 3640 932 2081 Fragments arranged by size: 3640 2081 932 47 StuI AGG'CCT Cuts at: 1517 3341 1517 Size: 1824 4876 StyI C'CwwG_G Cuts at: 513 1065 2074 3803 3841 4139 513 Size: 552 1009 1729 38 298 3074 Fragments arranged by size: 3074 1729 1009 552 298 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2992 3107 4727 4744 4897 5082 5527 Size: 1255 1412 115 1620 17 153 185 445 Cuts at: 5527 325 Size: 1498 Fragments arranged by size: 1620 1498 1412 1255 445 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2554 3591 3724 Size: 274 80 33 51 1443 577 1037 133 Cuts at: 3724 4946 6658 96 Size: 1222 1712 138 Fragments arranged by size: 1712 1443 1222 1037 577 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3345 3377 3840 3872 6660 6692 3345 Size: 32 463 32 2788 32 3353 Fragments arranged by size: 3353 2788 463 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6700 XbaI T'CTAG_A Cuts at: 4036 4036 Size: 6700 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6700 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 3296 3545 3812 4829 3296 Size: 249 267 1017 5167 Fragments arranged by size: 5167 1017 267 249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI XcmI