(Circular) (Six-base) MAPSORT of: pFN21ASDA0207.seq Check: 4742 from: 1 to: 6493 pFN21ASDA0207 6493 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3009 3009 Size: 6493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2357 3652 4556 5940 6208 1632 Size: 633 92 1295 904 1384 268 1917 Fragments arranged by size: 1917 1384 1295 904 633 268 92 AfeI AGC'GCT Cuts at: 2336 2336 Size: 6493 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 3331 5392 6369 1203 Size: 2128 2061 977 1327 Fragments arranged by size: 2128 2061 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AlwNI CAG_nnn'CTG Cuts at: 3010 4236 5808 3010 Size: 1226 1572 3695 Fragments arranged by size: 3695 1572 1226 ApaLI G'TGCA_C Cuts at: 2348 4550 5706 2348 Size: 2202 1156 3135 Fragments arranged by size: 3135 2202 1156 ApoI r'AATT_y Cuts at: 1493 2729 2782 2851 2880 3625 3692 3802 4028 Size: 1236 53 69 29 745 67 110 226 Cuts at: 4028 4180 6289 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1236 745 226 152 110 69 67 53 29 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2274 2317 3818 1870 Size: 73 331 43 1501 4545 Fragments arranged by size: 4545 1501 331 73 43 AvrII C'CTAG_G Cuts at: 2656 3418 2656 Size: 762 5731 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2367 2811 3814 5269 Size: 325 217 627 446 134 444 1003 1455 Cuts at: 5269 618 Size: 1842 Fragments arranged by size: 1842 1455 1003 627 446 444 325 217 134 BanII G_rGCy'C Cuts at: 1391 2456 3090 3540 3812 1391 Size: 1065 634 450 272 4072 Fragments arranged by size: 4072 1065 634 450 272 BbeI G_GCGC'C Cuts at: 2371 2371 Size: 6493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2388 2685 928 Size: 971 489 297 4736 Fragments arranged by size: 4736 971 489 297 BbvCI CC'TCA_GC Cuts at: 3131 3234 3131 Size: 103 6390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2057 2091 2165 2199 3256 3290 4684 Size: 34 120 34 74 34 1057 34 1394 Cuts at: 4684 4718 1903 Size: 34 3678 Fragments arranged by size: 3678 1394 1057 120 74 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 1548 Size: 6493 BfrBI ATG'CAT Cuts at: 2186 2186 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2951 3633 6488 2951 Size: 682 2855 2956 Fragments arranged by size: 2956 2855 682 BlpI GC'TnA_GC Cuts at: 3910 3910 Size: 6493 Bme1580I G_kGCm'C Cuts at: 1790 2238 2352 4554 5710 1790 Size: 448 114 2202 1156 2573 Fragments arranged by size: 2573 2202 1156 448 114 BmgBI CAC'GTC Cuts at: 1318 3168 1318 Size: 1850 4643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4224 5182 442 Size: 416 53 83 399 24 2807 958 1753 Fragments arranged by size: 2807 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2848 3041 3392 3409 3484 5153 1239 Size: 974 635 193 351 17 75 1669 2579 Fragments arranged by size: 2579 1669 974 635 351 193 75 17 Bpu10I CC'TnA_GC Cuts at: 3131 3234 3131 Size: 103 6390 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3179 3743 4618 5483 5781 3179 Size: 564 875 865 298 3891 Fragments arranged by size: 3891 875 865 564 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2571 5156 882 Size: 617 1072 2585 2219 Fragments arranged by size: 2585 2219 1072 617 BsaAI yAC'GTr Cuts at: 493 1204 3790 4284 493 Size: 711 2586 494 2702 Fragments arranged by size: 2702 2586 711 494 BsaBI GATnn'nnATC Cuts at: 3413 6487 3413 Size: 3074 3419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2368 3269 4300 4682 Size: 53 83 186 936 835 901 1031 382 Cuts at: 4682 5317 275 Size: 635 1451 Fragments arranged by size: 1451 1031 936 901 835 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4926 5324 5598 5745 6156 4926 Size: 398 274 147 411 5263 Fragments arranged by size: 5263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2186 2216 3023 Size: 30 165 30 688 30 65 30 807 Cuts at: 3023 3053 1178 Size: 30 4618 Fragments arranged by size: 4618 807 688 165 65 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2280 3182 3389 3548 1186 Size: 1094 902 207 159 4131 Fragments arranged by size: 4131 1094 902 207 159 BseYI C'CCAG_C Cuts at: 2340 2838 2908 5696 6192 2340 Size: 498 70 2788 496 2641 Fragments arranged by size: 2788 2641 498 496 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 3165 1163 Size: 573 615 814 4491 Fragments arranged by size: 4491 814 615 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3943 4704 5732 1540 Size: 275 186 1942 761 1028 2301 Fragments arranged by size: 2301 1942 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 2466 2646 4554 4639 5710 2352 Size: 114 180 1908 85 1071 3135 Fragments arranged by size: 3135 1908 1071 180 114 85 BsmI GAATG_Cn' Cuts at: 2188 3210 4004 4097 2188 Size: 1022 794 93 4584 Fragments arranged by size: 4584 1022 794 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2352 2456 2466 2646 3090 3540 Size: 399 448 114 104 10 180 444 450 Cuts at: 3540 3812 4554 4639 5710 1391 Size: 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 448 444 399 272 180 114 104 85 10 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3009 3848 844 Size: 2165 839 3489 Fragments arranged by size: 3489 2165 839 BsrBI CCG'CTC Cuts at: 1394 1823 4380 1394 Size: 429 2557 3507 Fragments arranged by size: 3507 2557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3312 4988 5162 65 Size: 1183 2064 1676 174 1396 Fragments arranged by size: 2064 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2410 5137 5324 1700 Size: 110 223 377 2727 187 2869 Fragments arranged by size: 2869 2727 377 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 3299 3539 4553 5565 3299 Size: 240 1014 1012 4227 Fragments arranged by size: 4227 1014 1012 240 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 794 Size: 1535 4958 BstBI TT'CG_AA Cuts at: 2007 5297 2007 Size: 3290 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstYI r'GATC_y Cuts at: 1972 2951 3495 3633 3823 4190 4575 4592 6044 Size: 979 544 138 190 367 385 17 1452 Cuts at: 6044 6130 6142 6488 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 979 544 385 367 346 190 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2712 3082 513 Size: 552 1647 370 3924 Fragments arranged by size: 3924 1647 552 370 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 3286 3766 4009 4803 4823 3286 Size: 480 243 794 20 4956 Fragments arranged by size: 4956 794 480 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2526 2561 529 Size: 35 1962 35 4461 Fragments arranged by size: 4461 1962 35 35 DraI TTT'AAA Cuts at: 2770 3797 2770 Size: 1027 5466 DraIII CAC_nnn'GTG Cuts at: 2614 3603 2614 Size: 989 5504 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 2412 2715 Size: 54 1425 50 275 18 421 161 303 Cuts at: 2715 3012 3940 4829 6458 8 Size: 297 928 889 1629 43 Fragments arranged by size: 1629 1425 928 889 421 303 297 275 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3940 1537 Size: 275 2128 4090 Fragments arranged by size: 4090 2128 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4423 1353 Size: 3070 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3365 5080 5454 5600 387 Size: 1064 138 1776 1715 374 146 1280 Fragments arranged by size: 1776 1715 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2265 2357 2848 3041 3392 3409 Size: 393 581 52 92 491 193 351 17 Cuts at: 3409 3484 3652 4556 5153 5940 6208 1239 Size: 75 168 904 597 787 268 1524 Fragments arranged by size: 1524 904 787 597 581 491 393 351 268 193 168 92 75 52 17 EcoNI CCTnn'n_nnAGG Cuts at: 2375 3374 2375 Size: 999 5494 EcoO109I rG'GnC_Cy Cuts at: 2285 2285 Size: 6493 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FspI TGC'GCA Cuts at: 3278 4999 3278 Size: 1721 4772 HaeII r_GCGC'y Cuts at: 1678 2338 2371 5640 1678 Size: 660 33 3269 2531 Fragments arranged by size: 3269 2531 660 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 3328 3360 5134 Size: 32 135 32 519 32 1205 32 1774 Cuts at: 5134 5166 5208 5240 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1774 1205 519 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2084 3718 3837 4083 669 Size: 988 281 146 1634 119 246 3079 Fragments arranged by size: 3079 1634 988 281 246 146 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KasI G'GCGC_C Cuts at: 2367 2367 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 2588 4092 2588 Size: 1504 4989 MluI A'CGCG_T Cuts at: 3331 6369 3331 Size: 3038 3455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 2717 10 Size: 54 1425 343 421 464 3786 Fragments arranged by size: 3786 1425 464 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 3776 4451 4810 4969 518 Size: 648 2241 369 675 359 159 2042 Fragments arranged by size: 2241 2042 675 648 369 359 159 MspA1I CmG'CkG Cuts at: 1606 2459 2628 3007 3712 3730 3909 4586 5734 Size: 853 169 379 705 18 179 677 1148 Cuts at: 5734 5979 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 853 705 677 379 245 179 169 18 NaeI GCC'GGC Cuts at: 2035 2412 2035 Size: 377 6116 NarI GG'CG_CC Cuts at: 2368 2368 Size: 6493 NcoI C'CATG_G Cuts at: 513 1065 2712 513 Size: 552 1647 4294 Fragments arranged by size: 4294 1647 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NgoMIV G'CCGG_C Cuts at: 2033 2410 2033 Size: 377 6116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NsiI A_TGCA'T Cuts at: 2188 2188 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 2221 2261 2900 3851 5396 1132 Size: 97 992 40 639 951 1545 2229 Fragments arranged by size: 2229 1545 992 951 639 97 40 PasI CC'CwG_GG Cuts at: 1348 2447 1348 Size: 1099 5394 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 2593 2689 1108 Size: 1485 96 4912 Fragments arranged by size: 4912 1485 96 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2389 2421 4562 4594 6075 6107 2389 Size: 32 2141 32 1481 32 2775 Fragments arranged by size: 2775 2141 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PstI C_TGCA'G Cuts at: 830 2216 2247 3006 3845 830 Size: 1386 31 759 839 3478 Fragments arranged by size: 3478 1386 839 759 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2628 3007 3712 3730 2628 Size: 379 705 18 5391 Fragments arranged by size: 5391 705 379 18 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SfcI C'TryA_G Cuts at: 826 1045 2212 2243 3002 3075 3841 4976 5657 Size: 219 1167 31 759 73 766 1135 681 Cuts at: 5657 5848 826 Size: 191 1471 Fragments arranged by size: 1471 1167 1135 766 759 681 219 191 73 31 SfoI GGC'GCC Cuts at: 2369 2369 Size: 6493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3194 3758 4597 5498 5760 820 Size: 197 926 1251 564 839 901 262 1553 Fragments arranged by size: 1553 1251 926 901 839 564 262 197 SnaBI TAC'GTA Cuts at: 493 3790 4284 493 Size: 3297 494 2702 Fragments arranged by size: 3297 2702 494 SpeI A'CTAG_T Cuts at: 152 2635 152 Size: 2483 4010 SphI G_CATG'C Cuts at: 1132 2221 2261 3851 1132 Size: 1089 40 1590 3774 Fragments arranged by size: 3774 1590 1089 40 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 3248 1517 Size: 1731 4762 StyI C'CwwG_G Cuts at: 513 1065 2656 2712 2973 3418 3932 513 Size: 552 1591 56 261 445 514 3074 Fragments arranged by size: 3074 1591 552 514 445 261 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 2283 4520 4537 4690 4875 5320 Size: 1255 559 144 2237 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 2237 1498 1255 559 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 3063 3455 Size: 274 80 33 51 1443 225 861 392 Cuts at: 3455 3592 4739 6451 96 Size: 137 1147 1712 138 Fragments arranged by size: 1712 1443 1147 861 392 274 225 138 137 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2822 2822 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 2786 4622 2786 Size: 1836 4657 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI