Restriction map for
pFN21ASDA0202
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 865 CGCG CG^CG
AfaI 771 GTAC GT^AC
AlwNI 1255 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1260 CCTGAGG CC^T[ACGT]AGG
Aor51HI 20 AGCGCT AGC^GCT
Asp700I 990 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 375 TGCGCA TGC^GCA
BamHI 446 GGATCC G^GATCC
BbsI 856 GAAGAC GAAGAC(2/6)
BglI 474 GCCCACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 856 GAAGAC GAAGAC(2/6)
BspDI 370 ATCGAT AT^CGAT
BspHI 689 TCATGA T^CATGA
BspMI 789 GCAGGT ACCTGC(4/8)
BsrBI 417 CCGCTC CCGCTC(-3/-3)
BstAPI 1230 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 865 CGCG CG^CG
BstXI 8 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1260 CCTGAGG CC^T[ACGT]AGG
ClaI 370 ATCGAT AT^CGAT
DraII 1217 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 94 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 20 AGCGCT AGC^GCT
Eco81I 1260 CCTGAGG CC^T[ACGT]AGG
EcoNI 1260 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1217 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 557 CCAAGG C^C[AT][AT]GG
FspI 375 TGCGCA TGC^GCA
HinfI 804 GAATC G^A[ACGT]TC
MseI 680 TTAA T^TAA
MslI 174 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 97 CAGCTG C[AC]G^C[GT]G
MvnI 865 CGCG CG^CG
NaeI 881 GCCGGC GCC^GGC
NgoMIV 881 GCCGGC G^CCGGC
NspI 235 GCATGC [GA]CATG^[CT]
PvuII 97 CAGCTG CAG^CTG
RcaI 689 TCATGA T^CATGA
RsaI 771 GTAC GT^AC
SapI 423 GCTCTTC GCTCTTC(1/4)
SmlI 289 CTCAAG C^T[CT][GA]AG
SphI 235 GCATGC GCATG^C
StuI 1232 AGGCCT AGG^CCT
StyI 557 CCAAGG C^C[AT][AT]GG
TfiI 804 GAATC G^A[AT]TC
Tru9I 680 TTAA T^TAA
Tsp509I 388 AATT ^AATT
XbaI 498 TCTAGA T^CTAGA
XmnI 990 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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BglII 501 AGATCT A^GATCT
638 AGATCT
BsaBI 600 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
834 GATGTTGATC
BsaI 154 GAGACC GGTCTC(1/5)
1180 GAGACC
BsgI 283 GTGCAG GTGCAG(16/14)
787 GTGCAG
BsmI 30 GAATGC GAATGC(1/-1)
693 GAATGC
EaeI 5 CGGCCA [CT]^GGCC[GA]
66 CGGCCA
FauI 828 GCGGG CCCGC(4/6)
1122 GCGGG
HincII 361 GTTGAC GT[CT]^[GA]AC
1045 GTCAAC
HindII 361 GTTGAC GT[CT]^[GA]AC
1045 GTCAAC
MaeII 281 ACGT A^CGT
452 ACGT
MamI 600 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
834 GATGTTGATC
SfaNI 307 GATGC GCATC(5/9)
373 GATGC
TspRI 176 CACTG CA[GC]TG[ACGT][ACGT]^
808 CACTG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EarI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI SacI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
Tsp45I TthHB8I XhoII