Restriction map for
pFN21ASDA0202
(Linear) (Six-base) MAPSORT of: KIAA0202.seq Check: 7251 from: 1 to: 1287
KIAA0202.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 780 1287
Size: 780 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 427 511 942 1096 1138 1287
Size: 111 316 84 431 154 42 149
Fragments arranged by size:
431 316 154 149 111 84 42
AfeI AGC'GCT
Cuts at: 0 22 1287
Size: 22 1265
AlwNI CAG_nnn'CTG
Cuts at: 0 1260 1287
Size: 1260 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 754 787 1287
Size: 754 33 500
Fragments arranged by size:
754 500 33
BamHI G'GATC_C
Cuts at: 0 446 1287
Size: 446 841
BanII G_rGCy'C
Cuts at: 0 47 587 896 1097 1287
Size: 47 540 309 201 190
Fragments arranged by size:
540 309 201 190 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1287
Size: 863 424
BglI GCCn_nnn'nGGC
Cuts at: 0 480 1287
Size: 480 807
BglII A'GATC_T
Cuts at: 0 501 638 1287
Size: 501 137 649
Fragments arranged by size:
649 501 137
Bme1580I G_kGCm'C
Cuts at: 0 485 1287
Size: 485 802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1287
Size: 42 1245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 253 1090 1192 1287
Size: 37 216 837 102 95
Fragments arranged by size:
837 216 102 95 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1287
Size: 274 1013
BsaI GGTCTCn'nnnn_
Cuts at: 0 148 1174 1287
Size: 148 1026 113
Fragments arranged by size:
1026 148 113
BsaBI GATnn'nnATC
Cuts at: 0 604 838 1287
Size: 604 234 449
Fragments arranged by size:
604 449 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1287
Size: 556 30 701
Fragments arranged by size:
701 556 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 745 931 1150 1189 1192 1287
Size: 415 330 186 219 39 3 95
Fragments arranged by size:
415 330 219 186 95 39 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 808 1287
Size: 304 504 479
Fragments arranged by size:
504 479 304
BsiHKAI G_wGCw'C
Cuts at: 0 587 765 896 1097 1115 1287
Size: 587 178 131 201 18 172
Fragments arranged by size:
587 201 178 172 131 18
BsmI GAATG_Cn'
Cuts at: 0 36 699 1287
Size: 36 663 588
Fragments arranged by size:
663 588 36
Bsp1286I G_dGCh'C
Cuts at: 0 47 485 587 765 896 1097 1115 1287
Size: 47 438 102 178 131 201 18 172
Fragments arranged by size:
438 201 178 172 131 102 47 18
BspHI T'CATG_A
Cuts at: 0 689 1287
Size: 689 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 780 1287
Size: 780 507
BsrBI CCG'CTC
Cuts at: 0 419 1287
Size: 419 868
BsrFI r'CCGG_y
Cuts at: 0 157 881 899 1287
Size: 157 724 18 388
Fragments arranged by size:
724 388 157 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1236 1287
Size: 1236 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 15 1287
Size: 15 1272
BstYI r'GATC_y
Cuts at: 0 410 446 501 638 1287
Size: 410 36 55 137 649
Fragments arranged by size:
649 410 137 55 36
Bsu36I CC'TnA_GG
Cuts at: 0 1261 1287
Size: 1261 26
BtgI C'CryG_G
Cuts at: 0 311 653 713 1287
Size: 311 342 60 574
Fragments arranged by size:
574 342 311 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 537 953 1287
Size: 537 416 334
Fragments arranged by size:
537 416 334
BtsI GCAGTG_nn'
Cuts at: 0 807 1287
Size: 807 480
ClaI AT'CG_AT
Cuts at: 0 371 1287
Size: 371 916
DrdI GACnn_nn'nnGTC
Cuts at: 0 100 1287
Size: 100 1187
EaeI y'GGCC_r
Cuts at: 0 5 66 1287
Size: 5 61 1221
Fragments arranged by size:
1221 61 5
EarI CTCTTCn'nnn_
Cuts at: 0 196 430 1019 1287
Size: 196 234 589 268
Fragments arranged by size:
589 268 234 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1287
Size: 403 884
EcoICRI GAG'CTC
Cuts at: 0 585 894 1095 1287
Size: 585 309 201 192
Fragments arranged by size:
585 309 201 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 111 253 427 511 942 1090 1096
Size: 37 74 142 174 84 431 148 6
Cuts at: 1096 1138 1192 1287
Size: 42 54 95
Fragments arranged by size:
431 174 148 142 95 84 74 54
42 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1264 1287
Size: 1264 23
EcoO109I rG'GnC_Cy
Cuts at: 0 1218 1287
Size: 1218 69
FspI TGC'GCA
Cuts at: 0 377 1287
Size: 377 910
HaeII r_GCGC'y
Cuts at: 0 24 906 1164 1287
Size: 24 882 258 123
Fragments arranged by size:
882 258 123 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 1287
Size: 556 32 699
Fragments arranged by size:
699 556 32
HincII GTy'rAC
Cuts at: 0 363 1047 1287
Size: 363 684 240
Fragments arranged by size:
684 363 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1287
Size: 893 394
MslI CAynn'nnrTG
Cuts at: 0 178 1287
Size: 178 1109
MspA1I CmG'CkG
Cuts at: 0 99 1287
Size: 99 1188
NaeI GCC'GGC
Cuts at: 0 883 1287
Size: 883 404
NgoMIV G'CCGG_C
Cuts at: 0 881 1287
Size: 881 406
NspI r_CATG'y
Cuts at: 0 239 1287
Size: 239 1048
PasI CC'CwG_GG
Cuts at: 0 60 776 1287
Size: 60 716 511
Fragments arranged by size:
716 511 60
PfoI T'CCnGG_A
Cuts at: 0 269 1287
Size: 269 1018
PstI C_TGCA'G
Cuts at: 0 398 1013 1226 1287
Size: 398 615 213 61
Fragments arranged by size:
615 398 213 61
PvuII CAG'CTG
Cuts at: 0 99 1287
Size: 99 1188
SacI G_AGCT'C
Cuts at: 0 587 896 1097 1287
Size: 587 309 201 190
Fragments arranged by size:
587 309 201 190
SapI GCTCTTCn'nnn_
Cuts at: 0 430 1287
Size: 430 857
SfcI C'TryA_G
Cuts at: 0 394 1009 1222 1287
Size: 394 615 213 65
Fragments arranged by size:
615 394 213 65
SmlI C'TyrA_G
Cuts at: 0 289 1287
Size: 289 998
SphI G_CATG'C
Cuts at: 0 239 1287
Size: 239 1048
StuI AGG'CCT
Cuts at: 0 1234 1287
Size: 1234 53
StyI C'CwwG_G
Cuts at: 0 557 1287
Size: 557 730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 102 1287
Size: 102 1185
XbaI T'CTAG_A
Cuts at: 0 498 1287
Size: 498 789
XmnI GAAnn'nnTTC
Cuts at: 0 994 1287
Size: 994 293
Enzymes that do cut:
AarI AcuI AfeI AlwNI BaeI BamHI BanII BbsI
BglI BglII Bme1580I BplI BpmI BpuEI BsaI BsaBI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI MslI
MspA1I NaeI NgoMIV NspI PasI PfoI PstI PvuII
SacI SapI SfcI SmlI SphI StuI StyI TaqII
XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI Bpu10I BsaAI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I CspCI DraI
DraIII EagI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI TstI Tth111I XcmI XhoI
XmaI ZraI