(Circular) (Six-base) MAPSORT of: pFN21ASDA0181.seq Check: 3907 from: 1 to: 10918 pFN21ASDA0181 10918 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3686 3724 3686 Size: 38 10880 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8728 278 Size: 53 83 186 936 7192 2468 Fragments arranged by size: 7192 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7210 8261 1937 Size: 5273 1051 4594 Fragments arranged by size: 5273 4594 1051 Acc65I G'GTAC_C Cuts at: 7786 8239 7786 Size: 453 10465 AclI AA'CG_TT Cuts at: 1563 6732 9045 9418 1563 Size: 5169 2313 373 3063 Fragments arranged by size: 5169 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4955 5442 5709 6405 6831 7599 8103 8133 Size: 3323 487 267 696 426 768 504 30 Cuts at: 8133 8981 10365 10633 1632 Size: 848 1384 268 1917 Fragments arranged by size: 3323 1917 1384 848 768 696 504 487 426 268 267 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10721 AflIII A'CryG_T Cuts at: 1203 5479 9817 10794 1203 Size: 4276 4338 977 1327 Fragments arranged by size: 4338 4276 1327 977 AgeI A'CCGG_T Cuts at: 9749 9749 Size: 10918 AhdI GACnn_n'nnGTC Cuts at: 7217 7419 9647 7217 Size: 202 2228 8488 Fragments arranged by size: 8488 2228 202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5292 1184 Size: 4108 6810 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5198 5230 5198 Size: 32 10886 AlwNI CAG_nnn'CTG Cuts at: 4321 4384 6356 6631 8661 10233 4321 Size: 63 1972 275 2030 1572 5006 Fragments arranged by size: 5006 2030 1972 1572 275 63 ApaI G_GGCC'C Cuts at: 3255 3872 3982 4038 4088 4401 5201 7709 3255 Size: 617 110 56 50 313 800 2508 6464 Fragments arranged by size: 6464 2508 800 617 313 110 56 50 ApaLI G'TGCA_C Cuts at: 8975 10131 8975 Size: 1156 9762 ApoI r'AATT_y Cuts at: 1493 2491 2925 3649 3780 4916 5581 5815 5953 Size: 998 434 724 131 1136 665 234 138 Cuts at: 5953 6064 6220 6307 6454 7142 7497 7927 8227 Size: 111 156 87 147 688 355 430 300 Cuts at: 8227 8453 8605 10714 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 998 724 688 665 434 430 355 300 234 226 156 152 147 138 131 111 87 AseI AT'TA_AT Cuts at: 160 1032 5790 9472 160 Size: 872 4758 3682 1606 Fragments arranged by size: 4758 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10918 AvaI C'yCGr_G Cuts at: 1870 1943 2325 2578 4018 4546 7708 8243 1870 Size: 73 382 253 1440 528 3162 535 4545 Fragments arranged by size: 4545 3162 1440 535 528 382 253 73 AvrII C'CTAG_G Cuts at: 3544 5807 3544 Size: 2263 8655 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7770 7803 7770 Size: 33 10885 BamHI G'GATC_C Cuts at: 2349 2674 3335 5146 6418 7886 8248 8615 2349 Size: 325 661 1811 1272 1468 362 367 4652 Fragments arranged by size: 4652 1811 1468 1272 661 367 362 325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2608 2956 3619 4099 5005 Size: 325 217 627 821 348 663 480 906 Cuts at: 5005 7786 8239 9694 618 Size: 2781 453 1455 1842 Fragments arranged by size: 2781 1842 1455 906 821 663 627 480 453 348 325 217 BanII G_rGCy'C Cuts at: 1391 2292 2694 2998 3255 3661 3872 3982 4038 Size: 901 402 304 257 406 211 110 56 Cuts at: 4038 4088 4401 4603 4676 5201 5375 6523 7614 Size: 50 313 202 73 525 174 1148 1091 Cuts at: 7614 7709 7800 8031 8237 1391 Size: 95 91 231 206 4072 Fragments arranged by size: 4072 1148 1091 901 525 406 402 313 304 257 231 211 206 202 174 110 95 91 73 56 50 BbeI G_GCGC'C Cuts at: 4103 4103 Size: 10918 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2094 6627 7263 928 Size: 971 159 36 4533 636 4583 Fragments arranged by size: 4583 4533 971 636 159 36 BbvCI CC'TCA_GC Cuts at: 3663 3839 5052 7354 7720 3663 Size: 176 1213 2302 366 6861 Fragments arranged by size: 6861 2302 1213 366 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3589 3623 6471 6505 9109 9143 1903 Size: 34 1652 34 2848 34 2604 34 3678 Fragments arranged by size: 3678 2848 2604 1652 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8810 10020 8810 Size: 1210 9708 BclI T'GATC_A Cuts at: 1548 4852 5833 1548 Size: 3304 981 6633 Fragments arranged by size: 6633 3304 981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 4396 7704 Size: 107 122 71 631 605 2457 267 3308 Cuts at: 7704 9529 136 Size: 1825 1525 Fragments arranged by size: 3308 2457 1825 1525 631 605 267 122 107 71 BglII A'GATC_T Cuts at: 4807 6556 10913 4807 Size: 1749 4357 4812 Fragments arranged by size: 4812 4357 1749 BlpI GC'TnA_GC Cuts at: 8335 8335 Size: 10918 Bme1580I G_kGCm'C Cuts at: 1790 2241 3032 3255 3872 3912 3982 4038 4088 Size: 451 791 223 617 40 70 56 50 Cuts at: 4088 4401 5201 7709 7715 8979 10135 1790 Size: 313 800 2508 6 1264 1156 2573 Fragments arranged by size: 2573 2508 1264 1156 800 791 617 451 313 223 70 56 50 40 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2929 4640 5070 Size: 416 53 83 399 24 1512 1711 430 Cuts at: 5070 6429 7445 8649 9607 442 Size: 1359 1016 1204 958 1753 Fragments arranged by size: 1753 1711 1512 1359 1204 1016 958 430 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7338 1056 Size: 6282 4636 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5428 7819 5428 Size: 2391 8527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2952 2994 3012 3077 4890 5997 6537 6558 Size: 1713 42 18 65 1813 1107 540 21 Cuts at: 6558 6735 7162 7260 8009 8140 9578 1239 Size: 177 427 98 749 131 1438 2579 Fragments arranged by size: 2579 1813 1713 1438 1107 749 540 427 177 131 98 65 42 21 18 Bpu10I CC'TnA_GC Cuts at: 3109 3663 3839 5052 7223 7354 7720 3109 Size: 554 176 1213 2171 131 366 6307 Fragments arranged by size: 6307 2171 1213 554 366 176 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3813 5535 7559 9043 9908 10206 3813 Size: 1722 2024 1484 865 298 4525 Fragments arranged by size: 4525 2024 1722 1484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9581 882 Size: 617 8082 2219 Fragments arranged by size: 8082 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 8215 8709 493 Size: 711 7011 494 2702 Fragments arranged by size: 7011 2702 711 494 BsaBI GATnn'nnATC Cuts at: 10912 10912 Size: 10918 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3125 4100 8725 9107 Size: 53 83 186 936 1592 975 4625 382 Cuts at: 9107 9742 275 Size: 635 1451 Fragments arranged by size: 4625 1592 1451 975 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9351 9749 10023 10170 10581 9351 Size: 398 274 147 411 9688 Fragments arranged by size: 9688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3075 3105 5198 5228 7258 Size: 30 165 30 1672 30 2093 30 2030 Cuts at: 7258 7288 7588 7618 1178 Size: 30 300 30 4478 Fragments arranged by size: 4478 2093 2030 1672 300 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2634 2672 2997 3216 3301 3310 3708 4974 Size: 1448 38 325 219 85 9 398 1266 Cuts at: 4974 6150 7889 1186 Size: 1176 1739 4215 Fragments arranged by size: 4215 1739 1448 1266 1176 398 325 219 85 38 9 BseYI C'CCAG_C Cuts at: 2782 2796 3767 3861 3981 4077 4326 4400 4416 Size: 14 971 94 120 96 249 74 16 Cuts at: 4416 4831 5565 5591 5711 7666 7730 7907 10121 Size: 415 734 26 120 1955 64 177 2214 Cuts at: 10121 10617 2782 Size: 496 3083 Fragments arranged by size: 3083 2214 1955 971 734 496 415 249 177 120 120 96 94 74 64 26 16 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4310 4541 7322 7584 1163 Size: 573 2574 231 2781 262 4497 Fragments arranged by size: 4497 2781 2574 573 262 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8368 9129 10157 1540 Size: 275 186 6367 761 1028 2301 Fragments arranged by size: 6367 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4603 6523 7750 7800 8979 9064 10135 4603 Size: 1920 1227 50 1179 85 1071 5386 Fragments arranged by size: 5386 1920 1227 1179 1071 85 50 BsmI GAATG_Cn' Cuts at: 3558 4368 4488 5229 5316 8429 8522 3558 Size: 810 120 741 87 3113 93 5954 Fragments arranged by size: 5954 3113 810 741 120 93 87 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2292 2694 2998 3032 3255 3661 Size: 399 451 51 402 304 34 223 406 Cuts at: 3661 3872 3912 3982 4038 4088 4401 4603 4676 Size: 211 40 70 56 50 313 202 73 Cuts at: 4676 5201 5375 6523 7614 7709 7715 7750 7800 Size: 525 174 1148 1091 95 6 35 50 Cuts at: 7800 8031 8237 8979 9064 10135 1391 Size: 231 206 742 85 1071 2174 Fragments arranged by size: 2174 1148 1091 1071 742 525 451 406 402 399 313 304 231 223 211 206 202 174 95 85 73 70 56 51 50 50 40 35 34 6 BspEI T'CCGG_A Cuts at: 10581 10581 Size: 10918 BspHI T'CATG_A Cuts at: 5133 8807 10537 5133 Size: 3674 1730 5514 Fragments arranged by size: 5514 3674 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3686 3724 4123 5284 6003 8152 8273 844 Size: 2842 38 399 1161 719 2149 121 3489 Fragments arranged by size: 3489 2842 2149 1161 719 399 121 38 BsrBI CCG'CTC Cuts at: 1394 1823 2341 8805 1394 Size: 429 518 6464 3507 Fragments arranged by size: 6464 3507 518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 9413 9587 65 Size: 1183 1428 6737 174 1396 Fragments arranged by size: 6737 1428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4926 7440 9562 9749 1700 Size: 110 3116 2514 2122 187 2869 Fragments arranged by size: 3116 2869 2514 2122 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10918 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10918 BssSI C'ACGA_G Cuts at: 7230 8978 9990 7230 Size: 1748 1012 8158 Fragments arranged by size: 8158 1748 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3907 4138 4528 6356 794 Size: 3113 231 390 1828 5356 Fragments arranged by size: 5356 3113 1828 390 231 BstBI TT'CG_AA Cuts at: 2007 9722 2007 Size: 7715 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10918 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3775 4031 4424 6163 7642 2029 Size: 1746 256 393 1739 1479 5305 Fragments arranged by size: 5305 1746 1739 1479 393 256 BstYI r'GATC_y Cuts at: 1972 2349 2674 2723 3335 4807 5146 6418 6556 Size: 377 325 49 612 1472 339 1272 138 Cuts at: 6556 6628 7886 8248 8615 9000 9017 10469 10555 Size: 72 1258 362 367 385 17 1452 86 Cuts at: 10555 10567 10913 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1472 1452 1272 1258 612 385 377 367 362 346 339 325 138 86 72 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2719 4125 6288 7341 2719 Size: 1406 2163 1053 6296 Fragments arranged by size: 6296 2163 1406 1053 BtgI C'CryG_G Cuts at: 513 1065 2022 2478 3114 3742 4567 513 Size: 552 957 456 636 628 825 6864 Fragments arranged by size: 6864 957 825 636 628 552 456 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5517 487 Size: 683 56 4291 5888 Fragments arranged by size: 5888 4291 683 56 BtsI GCAGTG_nn' Cuts at: 5251 5440 6620 7713 7958 8029 8434 9228 9248 Size: 189 1180 1093 245 71 405 794 20 Cuts at: 9248 5251 Size: 6921 Fragments arranged by size: 6921 1180 1093 794 405 245 189 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2149 2151 2184 2186 7816 7851 529 Size: 35 1585 2 33 2 5630 35 3596 Fragments arranged by size: 5630 3596 1585 35 35 33 2 2 DraI TTT'AAA Cuts at: 5820 8222 5820 Size: 2402 8516 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9925 809 Size: 507 8609 1802 Fragments arranged by size: 8609 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4388 4928 5602 Size: 54 1425 50 275 18 2558 540 674 Cuts at: 5602 6165 7492 8365 9254 10883 8 Size: 563 1327 873 889 1629 43 Fragments arranged by size: 2558 1629 1425 1327 889 873 674 563 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8365 1537 Size: 275 6553 4090 Fragments arranged by size: 6553 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3230 4839 5780 6438 6781 7377 7384 8079 Size: 1877 1609 941 658 343 596 7 695 Cuts at: 8079 8848 1353 Size: 769 3423 Fragments arranged by size: 3423 1877 1609 941 769 695 658 596 343 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5417 7795 9505 9879 10025 387 Size: 1064 138 3828 2378 1710 374 146 1280 Fragments arranged by size: 3828 2378 1710 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4601 6521 7798 4601 Size: 1920 1277 7721 Fragments arranged by size: 7721 1920 1277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2952 2994 3012 3077 4890 4955 5442 Size: 393 1320 42 18 65 1813 65 487 Cuts at: 5442 5709 5997 6405 6537 6558 6735 6831 7162 Size: 267 288 408 132 21 177 96 331 Cuts at: 7162 7260 7599 8009 8103 8133 8140 8981 9578 Size: 98 339 410 94 30 7 841 597 Cuts at: 9578 10365 10633 1239 Size: 787 268 1524 Fragments arranged by size: 1813 1524 1320 841 787 597 487 410 408 393 339 331 288 268 267 177 132 98 96 94 65 65 42 30 21 18 7 EcoNI CCTnn'n_nnAGG Cuts at: 7871 7871 Size: 10918 EcoO109I rG'GnC_Cy Cuts at: 2615 3251 3252 3262 3868 3978 4035 4084 4121 Size: 636 1 10 606 110 57 49 37 Cuts at: 4121 4334 4381 4397 4901 5197 5198 5273 6592 Size: 213 47 16 504 296 1 75 1319 Cuts at: 6592 7428 7727 2615 Size: 836 299 5806 Fragments arranged by size: 5806 1319 836 636 606 504 299 296 213 110 75 57 49 47 37 16 10 1 1 EcoRI G'AATT_C Cuts at: 2925 3649 3780 5953 6220 6307 8227 8605 2925 Size: 724 131 2173 267 87 1920 378 5238 Fragments arranged by size: 5238 2173 1920 724 378 267 131 87 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3082 6292 3082 Size: 3210 7708 FspI TGC'GCA Cuts at: 9424 9424 Size: 10918 HaeII r_GCGC'y Cuts at: 1678 4103 10065 1678 Size: 2425 5962 2531 Fragments arranged by size: 5962 2531 2425 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2341 2373 3520 Size: 32 135 32 521 32 216 32 1147 Cuts at: 3520 3552 4012 4044 4108 4140 4969 5001 5138 Size: 32 460 32 64 32 829 32 137 Cuts at: 5138 5170 6938 6970 7203 7235 7405 7437 7616 Size: 32 1768 32 233 32 170 32 179 Cuts at: 7616 7648 9559 9591 9633 9665 1373 Size: 32 1911 32 42 32 2626 Fragments arranged by size: 2626 1911 1768 1147 829 521 460 233 216 179 170 137 135 64 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 4687 6500 6900 7211 7389 Size: 988 281 1423 1326 1813 400 311 178 Cuts at: 7389 7506 8262 8508 669 Size: 117 756 246 3079 Fragments arranged by size: 3079 1813 1423 1326 988 756 400 311 281 246 178 117 HindIII A'AGCT_T Cuts at: 748 748 Size: 10918 HpaI GTT'AAC Cuts at: 3361 8508 3361 Size: 5147 5771 KasI G'GCGC_C Cuts at: 4099 4099 Size: 10918 KpnI G_GTAC'C Cuts at: 7790 8243 7790 Size: 453 10465 MfeI C'AATT_G Cuts at: 8517 8517 Size: 10918 MluI A'CGCG_T Cuts at: 10794 10794 Size: 10918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3428 4168 5876 10032 10216 1305 Size: 2123 740 1708 4156 184 2007 Fragments arranged by size: 4156 2123 2007 1708 740 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6167 7494 10 Size: 54 1425 343 4335 1327 3434 Fragments arranged by size: 4335 3434 1425 1327 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 3494 3696 6678 8876 9235 9394 Size: 648 1401 927 202 2982 2198 359 159 Cuts at: 9394 518 Size: 2042 Fragments arranged by size: 2982 2198 2042 1401 927 648 359 202 159 MspA1I CmG'CkG Cuts at: 1606 2336 2800 2983 3667 3883 4835 6959 8334 Size: 730 464 183 684 216 952 2124 1375 Cuts at: 8334 9011 10159 10404 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2124 2120 1375 1148 952 730 684 677 464 245 216 183 NarI GG'CG_CC Cuts at: 4100 4100 Size: 10918 NcoI C'CATG_G Cuts at: 513 1065 2022 2478 4567 513 Size: 552 957 456 2089 6864 Fragments arranged by size: 6864 2089 957 552 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 10918 NheI G'CTAG_C Cuts at: 1052 7334 1052 Size: 6282 4636 NotI GC'GGCC_GC Cuts at: 8365 8365 Size: 10918 NspI r_CATG'y Cuts at: 1132 1229 3695 3850 3895 4276 5003 5473 5483 Size: 97 2466 155 45 381 727 470 10 Cuts at: 5483 7518 8276 9821 1132 Size: 2035 758 1545 2229 Fragments arranged by size: 2466 2229 2035 1545 758 727 470 381 155 97 45 10 PasI CC'CwG_GG Cuts at: 1348 3016 3415 3644 3916 6053 6545 1348 Size: 1668 399 229 272 2137 492 5721 Fragments arranged by size: 5721 2137 1668 492 399 272 229 PciI A'CATG_T Cuts at: 5479 9817 5479 Size: 4338 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 4006 4117 5525 6053 1108 Size: 2898 111 1408 528 5973 Fragments arranged by size: 5973 2898 1408 528 111 PfoI T'CCnGG_A Cuts at: 2533 2533 Size: 10918 PmeI GTTT'AAAC Cuts at: 8222 8222 Size: 10918 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 8987 9019 10500 10532 5198 Size: 32 3757 32 1481 32 5584 Fragments arranged by size: 5584 3757 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3262 4381 5273 6592 7428 7727 3262 Size: 1119 892 1319 836 299 6453 Fragments arranged by size: 6453 1319 1119 892 836 299 PshAI GACnn'nnGTC Cuts at: 1613 4306 1613 Size: 2693 8225 PsiI TTA'TAA Cuts at: 8488 8488 Size: 10918 PspOMI G'GGCC_C Cuts at: 3251 3868 3978 4034 4084 4397 5197 7705 3251 Size: 617 110 56 50 313 800 2508 6464 Fragments arranged by size: 6464 2508 800 617 313 110 56 50 PstI C_TGCA'G Cuts at: 830 2634 2805 4714 6244 6627 7063 7965 8270 Size: 1804 171 1909 1530 383 436 902 305 Cuts at: 8270 830 Size: 3478 Fragments arranged by size: 3478 1909 1804 1530 902 436 383 305 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10918 PvuII CAG'CTG Cuts at: 2800 2983 3667 3883 4835 6959 2800 Size: 183 684 216 952 2124 6759 Fragments arranged by size: 6759 2124 952 684 216 183 SacI G_AGCT'C Cuts at: 4603 6523 7800 4603 Size: 1920 1277 7721 Fragments arranged by size: 7721 1920 1277 SalI G'TCGA_C Cuts at: 1936 7209 8260 1936 Size: 5273 1051 4594 Fragments arranged by size: 5273 4594 1051 SanDI GG'GwC_CC Cuts at: 3262 3262 Size: 10918 SapI GCTCTTCn'nnn_ Cuts at: 6781 8079 6781 Size: 1298 9620 SbfI CC_TGCA'GG Cuts at: 8270 8270 Size: 10918 ScaI AGT'ACT Cuts at: 5487 9166 10878 5487 Size: 3679 1712 5527 Fragments arranged by size: 5527 3679 1712 SexAI A'CCwGG_T Cuts at: 4933 4933 Size: 10918 SfcI C'TryA_G Cuts at: 826 1045 2630 2801 4440 4710 6240 6623 7059 Size: 219 1585 171 1639 270 1530 383 436 Cuts at: 7059 7961 8266 9401 10082 10273 826 Size: 902 305 1135 681 191 1471 Fragments arranged by size: 1639 1585 1530 1471 1135 902 681 436 383 305 270 219 191 171 SfiI GGCCn_nnn'nGGCC Cuts at: 4396 4396 Size: 10918 SfoI GGC'GCC Cuts at: 4101 4101 Size: 10918 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10918 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10918 SmaI CCC'GGG Cuts at: 1872 2327 2580 7710 8245 1872 Size: 455 253 5130 535 4545 Fragments arranged by size: 5130 4545 535 455 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 3828 5550 7538 9022 9923 10185 Size: 197 926 1885 1722 1988 1484 901 262 Cuts at: 10185 820 Size: 1553 Fragments arranged by size: 1988 1885 1722 1553 1484 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 8215 8709 493 Size: 7722 494 2702 Fragments arranged by size: 7722 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10918 SphI G_CATG'C Cuts at: 1132 3695 3895 8276 1132 Size: 2563 200 4381 3774 Fragments arranged by size: 4381 3774 2563 200 SrfI GCCC'GGGC Cuts at: 7710 7710 Size: 10918 SspI AAT'ATT Cuts at: 5 52 2226 5794 8842 5 Size: 47 2174 3568 3048 2081 Fragments arranged by size: 3568 3048 2174 2081 47 StuI AGG'CCT Cuts at: 1517 3543 5844 7967 1517 Size: 2026 2301 2123 4468 Fragments arranged by size: 4468 2301 2123 2026 StyI C'CwwG_G Cuts at: 513 1065 2022 2478 3046 3386 3544 4567 5192 Size: 552 957 456 568 340 158 1023 625 Cuts at: 5192 5334 5807 7509 7585 8357 513 Size: 142 473 1702 76 772 3074 Fragments arranged by size: 3074 1702 1023 957 772 625 568 552 473 456 340 158 142 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2760 4075 5710 7687 8945 8962 9115 Size: 1255 1180 1315 1635 1977 1258 17 153 Cuts at: 9115 9300 9745 325 Size: 185 445 1498 Fragments arranged by size: 1977 1635 1498 1315 1258 1255 1180 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2275 5485 9164 Size: 274 80 33 51 1443 298 3210 3679 Cuts at: 9164 10876 96 Size: 1712 138 Fragments arranged by size: 3679 3210 1712 1443 298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 3175 3207 4272 4304 5433 5465 6729 Size: 32 900 32 1065 32 1129 32 1264 Cuts at: 6729 6761 6817 6849 7588 7620 10878 10910 2243 Size: 32 56 32 739 32 3258 32 2251 Fragments arranged by size: 3258 2251 1264 1129 1065 900 739 56 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10918 XbaI T'CTAG_A Cuts at: 8254 8254 Size: 10918 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2987 3695 4408 5395 7984 2167 Size: 820 708 713 987 2589 5101 Fragments arranged by size: 5101 2589 987 820 713 708 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10918 XmaI C'CCGG_G Cuts at: 1870 2325 2578 7708 8243 1870 Size: 455 253 5130 535 4545 Fragments arranged by size: 5130 4545 535 455 253 XmnI GAAnn'nnTTC Cuts at: 5344 9047 5344 Size: 3703 7215 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8726 276 Size: 53 83 186 936 7192 2468 Fragments arranged by size: 7192 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SwaI