(Circular) (Six-base) MAPSORT of: pFN21ASDA0174.seq Check: 7843 from: 1 to: 5821 pFN21ASDA0174 5821 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2324 3164 1937 Size: 387 840 4594 Fragments arranged by size: 4594 840 387 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3078 3884 5268 5536 1632 Size: 1446 806 1384 268 1917 Fragments arranged by size: 1917 1446 1384 806 268 AfeI AGC'GCT Cuts at: 2051 2051 Size: 5821 AflII C'TTAA_G Cuts at: 820 1017 2053 820 Size: 197 1036 4588 Fragments arranged by size: 4588 1036 197 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3056 3564 5136 3056 Size: 508 1572 3741 Fragments arranged by size: 3741 1572 508 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 2062 3130 3356 3508 5617 1493 Size: 569 1068 226 152 2109 1697 Fragments arranged by size: 2109 1697 1068 569 226 152 AseI AT'TA_AT Cuts at: 160 1032 2880 4375 160 Size: 872 1848 1495 1606 Fragments arranged by size: 1848 1606 1495 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 BamHI G'GATC_C Cuts at: 2181 3151 3518 2181 Size: 970 367 4484 Fragments arranged by size: 4484 970 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 3038 3142 4597 618 Size: 325 217 627 917 334 104 1455 1842 Fragments arranged by size: 1842 1455 917 627 334 325 217 104 BanII G_rGCy'C Cuts at: 1391 2033 2179 3140 1391 Size: 642 146 961 4072 Fragments arranged by size: 4072 961 642 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 3066 928 Size: 971 315 852 3683 Fragments arranged by size: 3683 971 852 315 BbvCI CC'TCA_GC Cuts at: 3049 3049 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 2386 1548 Size: 838 4983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2602 5816 2602 Size: 3214 2607 BlpI GC'TnA_GC Cuts at: 2046 3238 2046 Size: 1192 4629 Bme1580I G_kGCm'C Cuts at: 1790 3882 5038 1790 Size: 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2336 4481 1239 Size: 1019 78 2145 2579 Fragments arranged by size: 2579 2145 1019 78 Bpu10I CC'TnA_GC Cuts at: 3049 3049 Size: 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3118 3612 493 Size: 711 1914 494 2702 Fragments arranged by size: 2702 1914 711 494 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2334 2574 2670 1186 Size: 1148 240 96 4337 Fragments arranged by size: 4337 1148 240 96 BseYI C'CCAG_C Cuts at: 2026 2159 5024 5520 2026 Size: 133 2865 496 2327 Fragments arranged by size: 2865 2327 496 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2889 1163 Size: 573 1153 4095 Fragments arranged by size: 4095 1153 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2179 2196 3882 3967 5038 2179 Size: 17 1686 85 1071 2962 Fragments arranged by size: 2962 1686 1071 85 17 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2179 2196 3140 3882 3967 5038 Size: 399 243 146 17 944 742 85 1071 Cuts at: 5038 1391 Size: 2174 Fragments arranged by size: 2174 1071 944 742 399 243 146 85 17 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2262 2971 3176 844 Size: 1418 709 205 3489 Fragments arranged by size: 3489 1418 709 205 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4465 4652 1700 Size: 110 2655 187 2869 Fragments arranged by size: 2869 2655 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 2216 3881 4893 2216 Size: 1665 1012 3144 Fragments arranged by size: 3144 1665 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 2007 4625 2007 Size: 2618 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2503 2503 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2181 2232 2602 3151 3518 3903 3920 5372 Size: 209 51 370 549 367 385 17 1452 Cuts at: 5372 5458 5470 5816 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 549 385 370 367 346 209 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2226 513 Size: 552 1161 4108 Fragments arranged by size: 4108 1161 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2759 3337 4131 4151 2759 Size: 578 794 20 4429 Fragments arranged by size: 4429 794 578 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 2041 3125 2041 Size: 1084 4737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2567 2984 4828 809 Size: 507 1251 417 1844 1802 Fragments arranged by size: 1844 1802 1251 507 417 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786 Size: 54 1425 50 275 18 1438 889 1629 Cuts at: 5786 8 Size: 43 Fragments arranged by size: 1629 1438 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2647 2905 3090 3751 1353 Size: 1294 258 185 661 3423 Fragments arranged by size: 3423 1294 661 258 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2177 2177 Size: 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2336 3078 3884 4481 5268 5536 Size: 393 626 78 742 806 597 787 268 Cuts at: 5536 1239 Size: 1524 Fragments arranged by size: 1524 806 787 742 626 597 393 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 2219 2652 2219 Size: 433 5388 EcoO109I rG'GnC_Cy Cuts at: 2645 2645 Size: 5821 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2053 4968 1678 Size: 375 2915 2531 Fragments arranged by size: 2915 2531 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 2902 2934 2987 Size: 32 135 32 594 32 704 32 53 Cuts at: 2987 3019 4462 4494 4536 4568 1373 Size: 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 704 594 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 2948 748 Size: 2200 3621 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2596 4935 5119 1305 Size: 1291 2339 184 2007 Fragments arranged by size: 2339 2007 1291 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 2726 3779 4138 4297 518 Size: 648 1554 6 1053 359 159 2042 Fragments arranged by size: 2042 1554 1053 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 3237 3914 5062 5307 1606 Size: 1631 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2226 513 Size: 552 1161 4108 Fragments arranged by size: 4108 1161 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2687 1108 Size: 1579 4242 PfoI T'CCnGG_A Cuts at: 2202 2235 2920 3083 2202 Size: 33 685 163 4940 Fragments arranged by size: 4940 685 163 33 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2353 1613 Size: 740 5081 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 2968 3173 830 Size: 2138 205 3478 Fragments arranged by size: 3478 2138 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SacI G_AGCT'C Cuts at: 2179 2179 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 3090 3090 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2868 2964 3169 4304 4985 5176 826 Size: 219 1823 96 205 1135 681 191 1471 Fragments arranged by size: 1823 1471 1135 681 219 205 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 3925 4826 5088 820 Size: 197 926 110 1872 901 262 1553 Fragments arranged by size: 1872 1553 926 901 262 197 110 SnaBI TAC'GTA Cuts at: 493 3118 3612 493 Size: 2625 494 2702 Fragments arranged by size: 2702 2625 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 2824 3745 5 Size: 47 2772 921 2081 Fragments arranged by size: 2772 2081 921 47 StuI AGG'CCT Cuts at: 1517 2813 1517 Size: 1296 4525 StyI C'CwwG_G Cuts at: 513 1065 2226 3260 513 Size: 552 1161 1034 3074 Fragments arranged by size: 3074 1161 1034 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 3848 3865 4018 4203 4648 325 Size: 1255 929 1339 17 153 185 445 1498 Fragments arranged by size: 1498 1339 1255 929 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 4067 5779 Size: 274 80 33 51 1443 267 1823 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1823 1712 1443 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 5781 5813 2484 Size: 32 3265 32 2492 Fragments arranged by size: 3265 2492 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2233 2503 2233 Size: 270 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI