(Circular) (Six-base) MAPSORT of: pFN21ASDA0171.seq Check: 2487 from: 1 to: 6604 pFN21ASDA0171 6604 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4414 278 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3947 1937 Size: 2010 4594 Acc65I G'GTAC_C Cuts at: 3925 3925 Size: 6604 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2940 2952 3267 4667 6051 6319 1632 Size: 1308 12 315 1400 1384 268 1917 Fragments arranged by size: 1917 1400 1384 1308 315 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 2031 5503 6480 1203 Size: 828 3472 977 1327 Fragments arranged by size: 3472 1327 977 828 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3455 1184 Size: 2271 4333 AlwNI CAG_nnn'CTG Cuts at: 3083 3176 4347 5919 3083 Size: 93 1171 1572 3768 Fragments arranged by size: 3768 1572 1171 93 ApaLI G'TGCA_C Cuts at: 4661 5817 4661 Size: 1156 5448 ApoI r'AATT_y Cuts at: 1493 2432 2768 3277 3343 3877 3913 4139 4291 Size: 939 336 509 66 534 36 226 152 Cuts at: 4291 6400 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 939 534 509 336 226 152 66 36 AseI AT'TA_AT Cuts at: 160 1032 5158 160 Size: 872 4126 1606 Fragments arranged by size: 4126 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 3929 1870 Size: 73 1986 4545 Fragments arranged by size: 4545 1986 73 BamHI G'GATC_C Cuts at: 3934 4301 3934 Size: 367 6237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3017 3698 3925 5380 618 Size: 325 217 627 1230 681 227 1455 1842 Fragments arranged by size: 1842 1455 1230 681 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2562 3308 3923 1391 Size: 1171 746 615 4072 Fragments arranged by size: 4072 1171 746 615 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 2946 928 Size: 971 804 243 4586 Fragments arranged by size: 4586 971 804 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4795 4829 1903 Size: 34 2858 34 3678 Fragments arranged by size: 3678 2858 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4496 5706 4496 Size: 1210 5394 BclI T'GATC_A Cuts at: 1548 1548 Size: 6604 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5215 136 Size: 107 122 71 631 605 3543 1525 Fragments arranged by size: 3543 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2753 6599 2753 Size: 3846 2758 BlpI GC'TnA_GC Cuts at: 3076 4021 3076 Size: 945 5659 Bme1580I G_kGCm'C Cuts at: 1790 4665 5821 1790 Size: 2875 1156 2573 Fragments arranged by size: 2875 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4335 5293 Size: 416 53 83 399 24 2039 879 958 Cuts at: 5293 442 Size: 1753 Fragments arranged by size: 2039 1753 958 879 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 3166 5264 1239 Size: 1449 478 2098 2579 Fragments arranged by size: 2579 2098 1449 478 Bpu10I CC'TnA_GC Cuts at: 2556 2556 Size: 6604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4729 5594 5892 4729 Size: 865 298 5441 Fragments arranged by size: 5441 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2822 5267 882 Size: 617 1323 2445 2219 Fragments arranged by size: 2445 2219 1323 617 BsaAI yAC'GTr Cuts at: 493 1204 3901 4395 493 Size: 711 2697 494 2702 Fragments arranged by size: 2702 2697 711 494 BsaBI GATnn'nnATC Cuts at: 6598 6598 Size: 6604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4411 4793 5428 275 Size: 53 83 186 936 2878 382 635 1451 Fragments arranged by size: 2878 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2679 5037 5435 5709 5856 6267 2679 Size: 2358 398 274 147 411 3016 Fragments arranged by size: 3016 2358 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6379 Fragments arranged by size: 6379 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 2798 1186 Size: 1408 204 4992 Fragments arranged by size: 4992 1408 204 BseYI C'CCAG_C Cuts at: 3516 3785 5807 6303 3516 Size: 269 2022 496 3817 Fragments arranged by size: 3817 2022 496 269 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6031 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4815 5843 1540 Size: 275 186 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3362 4665 4750 5821 3362 Size: 1303 85 1071 4145 Fragments arranged by size: 4145 1303 1071 85 BsmI GAATG_Cn' Cuts at: 2935 4115 4208 2935 Size: 1180 93 5331 Fragments arranged by size: 5331 1180 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2562 3308 3362 3923 4665 4750 5821 Size: 399 772 746 54 561 742 85 1071 Cuts at: 5821 1391 Size: 2174 Fragments arranged by size: 2174 1071 772 746 742 561 399 85 54 BspEI T'CCGG_A Cuts at: 2679 6267 2679 Size: 3588 3016 BspHI T'CATG_A Cuts at: 4493 6223 4493 Size: 1730 4874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 844 Size: 3115 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4491 1394 Size: 429 2668 3507 Fragments arranged by size: 3507 2668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5099 5273 65 Size: 1183 3851 174 1396 Fragments arranged by size: 3851 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5248 5435 1700 Size: 110 3438 187 2869 Fragments arranged by size: 3438 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6604 BstBI TT'CG_AA Cuts at: 2007 5408 2007 Size: 3401 3203 BstEII G'GTnAC_C Cuts at: 1184 2993 1184 Size: 1809 4795 BstXI CCAn_nnnn'nTGG Cuts at: 3709 3709 Size: 6604 BstYI r'GATC_y Cuts at: 1972 2753 3934 4301 4686 4703 6155 6241 6253 Size: 781 1181 367 385 17 1452 86 12 Cuts at: 6253 6599 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1181 781 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3702 513 Size: 552 2637 3415 Fragments arranged by size: 3415 2637 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2739 487 Size: 683 56 1513 4352 Fragments arranged by size: 4352 1513 683 56 BtsI GCAGTG_nn' Cuts at: 3211 4120 4914 4934 3211 Size: 909 794 20 4881 Fragments arranged by size: 4881 909 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2053 2088 529 Size: 35 1489 35 5045 Fragments arranged by size: 5045 1489 35 35 DraI TTT'AAA Cuts at: 3908 3908 Size: 6604 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5611 809 Size: 507 4295 1802 Fragments arranged by size: 4295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569 Size: 54 1425 50 275 18 2221 889 1629 Cuts at: 6569 8 Size: 43 Fragments arranged by size: 2221 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 1537 Size: 275 2239 4090 Fragments arranged by size: 4090 2239 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2248 2457 2740 2775 4534 1353 Size: 895 209 283 35 1759 3423 Fragments arranged by size: 3423 1759 895 283 209 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5191 5565 5711 387 Size: 1064 138 3602 374 146 1280 Fragments arranged by size: 3602 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 2940 2952 3166 3267 4667 5264 Size: 393 1056 252 12 214 101 1400 597 Cuts at: 5264 6051 6319 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1400 1056 787 597 393 268 252 214 101 12 EcoO109I rG'GnC_Cy Cuts at: 2135 3173 3662 2135 Size: 1038 489 5077 Fragments arranged by size: 5077 1038 489 EcoRI G'AATT_C Cuts at: 2768 3913 4291 2768 Size: 1145 378 5081 Fragments arranged by size: 5081 1145 378 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 5751 1678 Size: 4073 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2333 2365 2549 Size: 32 135 32 509 32 220 32 184 Cuts at: 2549 2581 3059 3091 5245 5277 5319 5351 1373 Size: 32 478 32 2154 32 42 32 2626 Fragments arranged by size: 2626 2154 509 478 220 184 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3948 4194 669 Size: 988 281 1092 918 246 3079 Fragments arranged by size: 3079 1092 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6604 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3929 3929 Size: 6604 MfeI C'AATT_G Cuts at: 2655 4203 2655 Size: 1548 5056 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2503 5718 5902 1305 Size: 1198 3215 184 2007 Fragments arranged by size: 3215 2007 1198 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4782 Fragments arranged by size: 4782 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2337 3671 3707 3761 3773 3833 4562 Size: 648 1171 1334 36 54 12 60 729 Cuts at: 4562 4921 5080 518 Size: 359 159 2042 Fragments arranged by size: 2042 1334 1171 729 648 359 159 60 54 36 12 MspA1I CmG'CkG Cuts at: 1606 3044 3080 3221 3782 4020 4697 5845 6090 Size: 1438 36 141 561 238 677 1148 245 Cuts at: 6090 1606 Size: 2120 Fragments arranged by size: 2120 1438 1148 677 561 245 238 141 36 NcoI C'CATG_G Cuts at: 513 1065 3702 513 Size: 552 2637 3415 Fragments arranged by size: 3415 2637 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NspI r_CATG'y Cuts at: 1132 1229 2035 2212 3625 3682 3823 3962 5507 Size: 97 806 177 1413 57 141 139 1545 Cuts at: 5507 1132 Size: 2229 Fragments arranged by size: 2229 1545 1413 806 177 141 139 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6604 PciI A'CATG_T Cuts at: 2031 5503 2031 Size: 3472 3132 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6604 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 3662 2135 Size: 1527 5077 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 2262 4174 2262 Size: 1912 4692 PstI C_TGCA'G Cuts at: 830 3956 830 Size: 3126 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 3044 3080 3044 Size: 36 6568 SalI G'TCGA_C Cuts at: 1936 3946 1936 Size: 2010 4594 SanDI GG'GwC_CC Cuts at: 3662 3662 Size: 6604 SapI GCTCTTCn'nnn_ Cuts at: 2457 2457 Size: 6604 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 4852 6564 4852 Size: 1712 4892 SexAI A'CCwGG_T Cuts at: 3493 3493 Size: 6604 SfcI C'TryA_G Cuts at: 826 1045 3952 5087 5768 5959 826 Size: 219 2907 1135 681 191 1471 Fragments arranged by size: 2907 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3931 1872 Size: 2059 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4708 5609 5871 820 Size: 197 926 2765 901 262 1553 Fragments arranged by size: 2765 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3901 4395 493 Size: 3408 494 2702 Fragments arranged by size: 3408 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6604 SphI G_CATG'C Cuts at: 1132 3682 3823 3962 1132 Size: 2550 141 139 3774 Fragments arranged by size: 3774 2550 141 139 SspI AAT'ATT Cuts at: 5 52 2404 4528 5 Size: 47 2352 2124 2081 Fragments arranged by size: 2352 2124 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6604 StyI C'CwwG_G Cuts at: 513 1065 2129 2166 3702 4043 513 Size: 552 1064 37 1536 341 3074 Fragments arranged by size: 3074 1536 1064 552 341 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4631 4648 4801 4986 5431 325 Size: 1255 3051 17 153 185 445 1498 Fragments arranged by size: 3051 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4850 6562 96 Size: 274 80 33 51 1443 2873 1712 138 Fragments arranged by size: 2873 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2567 2599 2845 2877 6564 6596 2567 Size: 32 246 32 3687 32 2575 Fragments arranged by size: 3687 2575 246 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6604 XbaI T'CTAG_A Cuts at: 3940 3940 Size: 6604 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6604 XmaI C'CCGG_G Cuts at: 1870 3929 1870 Size: 2059 4545 XmnI GAAnn'nnTTC Cuts at: 2190 4733 2190 Size: 2543 4061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4412 276 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI