(Circular) (Six-base) MAPSORT of: pFN21ASDA0167.seq Check: 4651 from: 1 to: 7237 pFN21ASDA0167 7237 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3408 3408 Size: 7237 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5047 278 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4530 4580 1937 Size: 2593 50 4594 Fragments arranged by size: 4594 2593 50 Acc65I G'GTAC_C Cuts at: 4558 4558 Size: 7237 AclI AA'CG_TT Cuts at: 1563 5364 5737 1563 Size: 3801 373 3063 Fragments arranged by size: 3801 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2434 3425 3567 3588 5300 6684 6952 1632 Size: 802 991 142 21 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 991 802 268 142 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7040 AflIII A'CryG_T Cuts at: 1203 6136 7113 1203 Size: 4933 977 1327 Fragments arranged by size: 4933 1327 977 AgeI A'CCGG_T Cuts at: 6068 6068 Size: 7237 AhdI GACnn_n'nnGTC Cuts at: 5966 5966 Size: 7237 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2774 1184 Size: 1590 5647 AlwNI CAG_nnn'CTG Cuts at: 2314 2435 2740 2906 4078 4980 6552 2314 Size: 121 305 166 1172 902 1572 2999 Fragments arranged by size: 2999 1572 1172 902 305 166 121 ApaI G_GGCC'C Cuts at: 3808 3808 Size: 7237 ApaLI G'TGCA_C Cuts at: 5294 6450 5294 Size: 1156 6081 ApoI r'AATT_y Cuts at: 1493 3418 3781 4546 4772 4924 7033 1493 Size: 1925 363 765 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 765 363 226 152 AseI AT'TA_AT Cuts at: 160 1032 5791 160 Size: 872 4759 1606 Fragments arranged by size: 4759 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 AvaI C'yCGr_G Cuts at: 1870 1943 2586 3220 3235 3426 4399 4562 1870 Size: 73 643 634 15 191 973 163 4545 Fragments arranged by size: 4545 973 643 634 191 163 73 15 BamHI G'GATC_C Cuts at: 4567 4934 4567 Size: 367 6870 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2401 3839 4087 4558 6013 Size: 325 217 627 614 1438 248 471 1455 Cuts at: 6013 618 Size: 1842 Fragments arranged by size: 1842 1455 1438 627 614 471 325 248 217 BanII G_rGCy'C Cuts at: 1391 2617 2673 2883 3282 3433 3470 3808 4257 Size: 1226 56 210 399 151 37 338 449 Cuts at: 4257 4556 1391 Size: 299 4072 Fragments arranged by size: 4072 1226 449 399 338 299 210 151 56 37 BbeI G_GCGC'C Cuts at: 3843 4091 3843 Size: 248 6989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 2440 2680 928 Size: 971 428 113 240 5485 Fragments arranged by size: 5485 971 428 240 113 BbvCI CC'TCA_GC Cuts at: 3262 3434 3262 Size: 172 7065 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2879 2913 5428 5462 1903 Size: 34 942 34 2515 34 3678 Fragments arranged by size: 3678 2515 942 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5129 6339 5129 Size: 1210 6027 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 6595 BfrBI ATG'CAT Cuts at: 2026 2026 Size: 7237 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 3758 4142 Size: 107 122 71 631 605 421 1665 384 Cuts at: 4142 4352 5848 136 Size: 210 1496 1525 Fragments arranged by size: 1665 1525 1496 631 605 421 384 210 122 107 71 BglII A'GATC_T Cuts at: 7232 7232 Size: 7237 BlpI GC'TnA_GC Cuts at: 4094 4654 4094 Size: 560 6677 Bme1580I G_kGCm'C Cuts at: 1790 2404 3808 3887 5298 6454 1790 Size: 614 1404 79 1411 1156 2573 Fragments arranged by size: 2573 1411 1404 1156 614 79 BmgBI CAC'GTC Cuts at: 1318 3615 4153 4264 4303 1318 Size: 2297 538 111 39 4252 Fragments arranged by size: 4252 2297 538 111 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 3354 4968 Size: 416 53 83 399 24 1727 210 1614 Cuts at: 4968 5926 442 Size: 958 1753 Fragments arranged by size: 1753 1727 1614 958 416 399 210 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4497 1056 Size: 3441 3796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2336 2477 3477 4370 5897 1239 Size: 890 207 141 1000 893 1527 2579 Fragments arranged by size: 2579 1527 1000 893 890 207 141 Bpu10I CC'TnA_GC Cuts at: 3262 3434 3464 3262 Size: 172 30 7035 Fragments arranged by size: 7035 172 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5362 6227 6525 5362 Size: 865 298 6074 Fragments arranged by size: 6074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3453 5900 882 Size: 617 1954 2447 2219 Fragments arranged by size: 2447 2219 1954 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 4534 5028 493 Size: 711 2615 715 494 2702 Fragments arranged by size: 2702 2615 715 711 494 BsaBI GATnn'nnATC Cuts at: 7231 7231 Size: 7237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3840 4088 4317 4512 Size: 53 83 186 936 2307 248 229 195 Cuts at: 4512 5044 5426 6061 275 Size: 532 382 635 1451 Fragments arranged by size: 2307 1451 936 635 532 382 248 229 195 186 83 53 BsaWI w'CCGG_w Cuts at: 2794 3414 3765 5670 6068 6342 6489 6900 2794 Size: 620 351 1905 398 274 147 411 3131 Fragments arranged by size: 3131 1905 620 411 398 351 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4031 4061 1178 Size: 30 165 30 2628 30 4354 Fragments arranged by size: 4354 2628 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2544 3529 3656 4052 4121 1186 Size: 1358 985 127 396 69 4302 Fragments arranged by size: 4302 1358 985 396 127 69 BseYI C'CCAG_C Cuts at: 2248 2396 2424 2762 3353 3557 4114 4430 4448 Size: 148 28 338 591 204 557 316 18 Cuts at: 4448 4466 6440 6936 2248 Size: 18 1974 496 2549 Fragments arranged by size: 2549 1974 591 557 496 338 316 204 148 28 18 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 4160 1163 Size: 573 1179 1245 4240 Fragments arranged by size: 4240 1245 1179 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4251 4687 5448 6476 1540 Size: 275 186 2250 436 761 1028 2301 Fragments arranged by size: 2301 2250 1028 761 436 275 186 BsiHKAI G_wGCw'C Cuts at: 2617 2883 2918 3299 4100 4257 5298 5383 6454 Size: 266 35 381 801 157 1041 85 1071 Cuts at: 6454 2617 Size: 3400 Fragments arranged by size: 3400 1071 1041 801 381 266 157 85 35 BsmI GAATG_Cn' Cuts at: 2175 4748 4841 2175 Size: 2573 93 4571 Fragments arranged by size: 4571 2573 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2495 2495 Size: 7237 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2404 2617 2673 2883 2918 3282 3299 Size: 399 614 213 56 210 35 364 17 Cuts at: 3299 3433 3470 3808 3887 4100 4257 4556 5298 Size: 134 37 338 79 213 157 299 742 Cuts at: 5298 5383 6454 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 399 364 338 299 213 213 210 157 134 85 79 56 37 35 17 BspEI T'CCGG_A Cuts at: 3765 6900 3765 Size: 3135 4102 BspHI T'CATG_A Cuts at: 5126 6856 5126 Size: 1730 5507 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3408 4592 844 Size: 2564 1184 3489 Fragments arranged by size: 3489 2564 1184 BsrBI CCG'CTC Cuts at: 1394 1823 2220 3646 4198 5124 1394 Size: 429 397 1426 552 926 3507 Fragments arranged by size: 3507 1426 926 552 429 397 BsrDI GCAATG_nn' Cuts at: 65 1248 3701 5732 5906 65 Size: 1183 2453 2031 174 1396 Fragments arranged by size: 2453 2031 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5881 6068 1700 Size: 110 4071 187 2869 Fragments arranged by size: 4071 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7237 BssSI C'ACGA_G Cuts at: 2604 5297 6309 2604 Size: 2693 1012 3532 Fragments arranged by size: 3532 2693 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2408 4282 794 Size: 1614 1874 3749 Fragments arranged by size: 3749 1874 1614 BstBI TT'CG_AA Cuts at: 2007 6041 2007 Size: 4034 3203 BstEII G'GTnAC_C Cuts at: 1184 2713 2870 1184 Size: 1529 157 5551 Fragments arranged by size: 5551 1529 157 BstYI r'GATC_y Cuts at: 1972 3678 4567 4934 5319 5336 6788 6874 6886 Size: 1706 889 367 385 17 1452 86 12 Cuts at: 6886 7232 1972 Size: 346 1977 Fragments arranged by size: 1977 1706 1452 889 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4531 4531 Size: 7237 Bsu36I CC'TnA_GG Cuts at: 2108 2108 Size: 7237 BtgI C'CryG_G Cuts at: 513 1065 2494 3178 3498 3747 3929 513 Size: 552 1429 684 320 249 182 3821 Fragments arranged by size: 3821 1429 684 552 320 249 182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2259 2268 487 Size: 683 56 1033 9 5456 Fragments arranged by size: 5456 1033 683 56 9 BtsI GCAGTG_nn' Cuts at: 2061 2907 4753 5547 5567 2061 Size: 846 1846 794 20 3731 Fragments arranged by size: 3731 1846 846 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7237 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3774 3809 529 Size: 35 3210 35 3957 Fragments arranged by size: 3957 3210 35 35 DraI TTT'AAA Cuts at: 4541 4541 Size: 7237 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6244 809 Size: 507 4928 1802 Fragments arranged by size: 4928 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2803 3750 Size: 54 1425 50 275 18 255 718 947 Cuts at: 3750 3926 3972 4248 4419 4437 4684 5573 7202 Size: 176 46 276 171 18 247 889 1629 Cuts at: 7202 8 Size: 43 Fragments arranged by size: 1629 1425 947 889 718 276 275 255 247 176 171 54 50 46 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4248 4684 1537 Size: 275 2436 436 4090 Fragments arranged by size: 4090 2436 436 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2845 3564 4033 5167 1353 Size: 1492 719 469 1134 3423 Fragments arranged by size: 3423 1492 1134 719 469 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5824 6198 6344 387 Size: 1064 138 4235 374 146 1280 Fragments arranged by size: 4235 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2615 2881 4255 2615 Size: 266 1374 5597 Fragments arranged by size: 5597 1374 266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2336 2434 2477 3425 3477 3567 Size: 393 497 207 98 43 948 52 90 Cuts at: 3567 3588 4370 5300 5897 6684 6952 1239 Size: 21 782 930 597 787 268 1524 Fragments arranged by size: 1524 948 930 787 782 597 497 393 268 207 98 90 52 43 21 EcoO109I rG'GnC_Cy Cuts at: 2925 3804 3805 4216 2925 Size: 879 1 411 5946 Fragments arranged by size: 5946 879 411 1 EcoRI G'AATT_C Cuts at: 3781 4546 4924 3781 Size: 765 378 6094 Fragments arranged by size: 6094 765 378 FspI TGC'GCA Cuts at: 2723 3725 5743 2723 Size: 1002 2018 4217 Fragments arranged by size: 4217 2018 1002 HaeII r_GCGC'y Cuts at: 1678 3843 4091 4337 4491 6384 1678 Size: 2165 248 246 154 1893 2531 Fragments arranged by size: 2531 2165 1893 248 246 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2052 2084 3374 3406 4031 Size: 32 135 32 480 32 1290 32 625 Cuts at: 4031 4063 5878 5910 5952 5984 1373 Size: 32 1815 32 42 32 2626 Fragments arranged by size: 2626 1815 1290 625 480 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4581 4827 669 Size: 988 281 2643 246 3079 Fragments arranged by size: 3079 2643 988 281 246 HindIII A'AGCT_T Cuts at: 748 2998 748 Size: 2250 4987 HpaI GTT'AAC Cuts at: 4827 4827 Size: 7237 KasI G'GCGC_C Cuts at: 3839 4087 3839 Size: 248 6989 KpnI G_GTAC'C Cuts at: 4562 4562 Size: 7237 MfeI C'AATT_G Cuts at: 4836 4836 Size: 7237 MluI A'CGCG_T Cuts at: 7113 7113 Size: 7237 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6351 6535 1305 Size: 5046 184 2007 Fragments arranged by size: 5046 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2087 2805 3752 3928 10 Size: 54 1425 343 255 718 947 176 3319 Fragments arranged by size: 3319 1425 947 718 343 255 176 54 MslI CAynn'nnrTG Cuts at: 518 1166 5195 5554 5713 518 Size: 648 4029 359 159 2042 Fragments arranged by size: 4029 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2503 2783 2897 4093 4114 4126 4225 4366 Size: 897 280 114 1196 21 12 99 141 Cuts at: 4366 4653 5330 6478 6723 1606 Size: 287 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 897 677 287 280 245 141 114 99 21 12 NarI GG'CG_CC Cuts at: 3840 4088 3840 Size: 248 6989 NcoI C'CATG_G Cuts at: 513 1065 2494 3498 513 Size: 552 1429 1004 4252 Fragments arranged by size: 4252 1429 1004 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7237 NheI G'CTAG_C Cuts at: 1052 4493 1052 Size: 3441 3796 NotI GC'GGCC_GC Cuts at: 4684 4684 Size: 7237 NsiI A_TGCA'T Cuts at: 2028 2028 Size: 7237 NspI r_CATG'y Cuts at: 1132 1229 2030 4595 6140 1132 Size: 97 801 2565 1545 2229 Fragments arranged by size: 2565 2229 1545 801 97 PasI CC'CwG_GG Cuts at: 1348 2921 4321 1348 Size: 1573 1400 4264 Fragments arranged by size: 4264 1573 1400 PciI A'CATG_T Cuts at: 6136 6136 Size: 7237 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 2698 2710 4153 1108 Size: 1206 384 12 1443 4192 Fragments arranged by size: 4192 1443 1206 384 12 PfoI T'CCnGG_A Cuts at: 2145 2691 2145 Size: 546 6691 PmeI GTTT'AAAC Cuts at: 4541 4541 Size: 7237 PmlI CAC'GTG Cuts at: 3819 3819 Size: 7237 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5306 5338 6819 6851 5306 Size: 32 1481 32 5692 Fragments arranged by size: 5692 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2925 4216 2925 Size: 1291 5946 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7237 PsiI TTA'TAA Cuts at: 4807 4807 Size: 7237 PspOMI G'GGCC_C Cuts at: 3804 3804 Size: 7237 PstI C_TGCA'G Cuts at: 830 2056 2642 2902 3372 4589 830 Size: 1226 586 260 470 1217 3478 Fragments arranged by size: 3478 1226 1217 586 470 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7237 PvuII CAG'CTG Cuts at: 2503 2783 2897 4126 4225 4366 2503 Size: 280 114 1229 99 141 5374 Fragments arranged by size: 5374 1229 280 141 114 99 SacI G_AGCT'C Cuts at: 2617 2883 4257 2617 Size: 266 1374 5597 Fragments arranged by size: 5597 1374 266 SalI G'TCGA_C Cuts at: 1936 4579 1936 Size: 2643 4594 SanDI GG'GwC_CC Cuts at: 2925 2925 Size: 7237 SbfI CC_TGCA'GG Cuts at: 4589 4589 Size: 7237 ScaI AGT'ACT Cuts at: 5485 7197 5485 Size: 1712 5525 SexAI A'CCwGG_T Cuts at: 2307 2307 Size: 7237 SfcI C'TryA_G Cuts at: 826 1045 2052 2638 2898 3368 4585 5720 6401 Size: 219 1007 586 260 470 1217 1135 681 Cuts at: 6401 6592 826 Size: 191 1471 Fragments arranged by size: 1471 1217 1135 1007 681 586 470 260 219 191 SfoI GGC'GCC Cuts at: 3841 4089 3841 Size: 248 6989 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7237 SmaI CCC'GGG Cuts at: 1872 3222 4401 4564 1872 Size: 1350 1179 163 4545 Fragments arranged by size: 4545 1350 1179 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 5341 6242 6504 820 Size: 197 926 3398 901 262 1553 Fragments arranged by size: 3398 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4534 5028 493 Size: 4041 494 2702 Fragments arranged by size: 4041 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7237 SphI G_CATG'C Cuts at: 1132 2030 4595 1132 Size: 898 2565 3774 Fragments arranged by size: 3774 2565 898 SspI AAT'ATT Cuts at: 5 52 5161 5 Size: 47 5109 2081 Fragments arranged by size: 5109 2081 47 StuI AGG'CCT Cuts at: 1517 2162 2535 3318 1517 Size: 645 373 783 5436 Fragments arranged by size: 5436 783 645 373 StyI C'CwwG_G Cuts at: 513 1065 2494 2541 3498 3774 4676 513 Size: 552 1429 47 957 276 902 3074 Fragments arranged by size: 3074 1429 957 902 552 276 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 3299 5264 5281 5434 5619 6064 Size: 1255 651 1068 1965 17 153 185 445 Cuts at: 6064 325 Size: 1498 Fragments arranged by size: 1965 1498 1255 1068 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 5483 7195 Size: 274 80 33 51 1443 360 3146 1712 Cuts at: 7195 96 Size: 138 Fragments arranged by size: 3146 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7197 7229 7197 Size: 32 7205 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7237 XbaI T'CTAG_A Cuts at: 4573 4573 Size: 7237 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3832 3832 Size: 7237 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7237 XmaI C'CCGG_G Cuts at: 1870 3220 4399 4562 1870 Size: 1350 1179 163 4545 Fragments arranged by size: 4545 1350 1179 163 XmnI GAAnn'nnTTC Cuts at: 2471 5366 2471 Size: 2895 4342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5045 276 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI