(Circular) (Six-base) MAPSORT of: pFN21ASDA0158.seq Check: 242 from: 1 to: 5812 pFN21ASDA0158 5812 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 2406 3133 2406 Size: 727 5085 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 2627 2864 3875 5259 5527 1632 Size: 977 18 237 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1011 977 268 237 18 AflII C'TTAA_G Cuts at: 820 1017 2501 820 Size: 197 1484 4131 Fragments arranged by size: 4131 1484 197 AflIII A'CryG_T Cuts at: 1203 3102 4711 5688 1203 Size: 1899 1609 977 1327 Fragments arranged by size: 1899 1609 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3092 3137 1870 Size: 73 1149 45 4545 Fragments arranged by size: 4545 1149 73 45 BamHI G'GATC_C Cuts at: 2744 3142 3509 2744 Size: 398 367 5047 Fragments arranged by size: 5047 398 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2406 3096 3133 4588 618 Size: 325 217 627 619 690 37 1455 1842 Fragments arranged by size: 1842 1455 690 627 619 325 217 37 BanII G_rGCy'C Cuts at: 1391 3009 3091 3131 1391 Size: 1618 82 40 4072 Fragments arranged by size: 4072 1618 82 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4841 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2982 5807 2982 Size: 2825 2987 BlpI GC'TnA_GC Cuts at: 3003 3229 3003 Size: 226 5586 Bme1580I G_kGCm'C Cuts at: 1790 3099 3873 5029 1790 Size: 1309 774 1156 2573 Fragments arranged by size: 2573 1309 1156 774 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2724 1056 Size: 1668 4144 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2807 2807 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2250 2867 4472 1239 Size: 1011 617 1605 2579 Fragments arranged by size: 2579 1605 1011 617 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 2918 3937 4802 5100 2699 Size: 219 1019 865 298 3411 Fragments arranged by size: 3411 1019 865 298 219 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3103 3109 3603 493 Size: 711 1899 6 494 2702 Fragments arranged by size: 2702 1899 711 494 6 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4001 4636 275 Size: 53 83 186 936 2086 382 635 1451 Fragments arranged by size: 2086 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5812 BseYI C'CCAG_C Cuts at: 5015 5511 5015 Size: 496 5316 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2832 3009 3873 3958 5029 2832 Size: 177 864 85 1071 3615 Fragments arranged by size: 3615 1071 864 177 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2832 3009 3091 3099 3131 3873 3958 Size: 399 1042 177 82 8 32 742 85 Cuts at: 3958 5029 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1042 742 399 177 85 82 32 8 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2418 3167 844 Size: 1574 749 3489 Fragments arranged by size: 3489 1574 749 BsrBI CCG'CTC Cuts at: 1394 1823 2424 2602 3699 1394 Size: 429 601 178 1097 3507 Fragments arranged by size: 3507 1097 601 429 178 BsrDI GCAATG_nn' Cuts at: 65 1248 2562 4307 4481 65 Size: 1183 1314 1745 174 1396 Fragments arranged by size: 1745 1396 1314 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5812 BstBI TT'CG_AA Cuts at: 2007 4616 2007 Size: 2609 3203 BstEII G'GTnAC_C Cuts at: 1184 2890 1184 Size: 1706 4106 BstYI r'GATC_y Cuts at: 1972 2744 2878 2982 3142 3509 3894 3911 5363 Size: 772 134 104 160 367 385 17 1452 Cuts at: 5363 5449 5461 5807 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 772 385 367 346 160 134 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5260 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3093 487 Size: 683 56 1867 3206 Fragments arranged by size: 3206 1867 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 2695 3116 2695 Size: 421 5391 DraIII CAC_nnn'GTG Cuts at: 3103 3103 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777 Size: 54 1425 50 275 18 1429 889 1629 Cuts at: 5777 8 Size: 43 Fragments arranged by size: 1629 1429 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 3742 1353 Size: 930 1459 3423 Fragments arranged by size: 3423 1459 930 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2999 4399 4773 4919 387 Size: 1064 138 1410 1400 374 146 1280 Fragments arranged by size: 1410 1400 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3007 3007 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2609 2627 2864 2867 3875 4472 Size: 393 618 359 18 237 3 1008 597 Cuts at: 4472 5259 5527 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 618 597 393 359 268 237 18 3 EcoO109I rG'GnC_Cy Cuts at: 2899 2899 Size: 5812 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2657 2689 4453 4485 4527 Size: 32 135 32 1085 32 1764 32 42 Cuts at: 4527 4559 1373 Size: 32 2626 Fragments arranged by size: 2626 1764 1085 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 3402 669 Size: 988 281 1218 246 3079 Fragments arranged by size: 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 2410 3137 2410 Size: 727 5085 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2056 2722 4926 5110 1305 Size: 751 666 2204 184 2007 Fragments arranged by size: 2204 2007 751 666 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2870 3770 4129 4288 518 Size: 648 975 729 900 359 159 2042 Fragments arranged by size: 2042 975 900 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3228 3905 5053 5298 1606 Size: 1622 677 1148 245 2120 Fragments arranged by size: 2120 1622 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5260 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 2720 1052 Size: 1668 4144 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2875 3019 3170 4715 1132 Size: 97 1646 144 151 1545 2229 Fragments arranged by size: 2229 1646 1545 151 144 97 PasI CC'CwG_GG Cuts at: 1348 2799 1348 Size: 1451 4361 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PfoI T'CCnGG_A Cuts at: 2883 2883 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PmlI CAC'GTG Cuts at: 3103 3103 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2899 2899 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 2051 3382 2051 Size: 1331 4481 PstI C_TGCA'G Cuts at: 830 2357 3164 830 Size: 1527 807 3478 Fragments arranged by size: 3478 1527 807 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 SacI G_AGCT'C Cuts at: 3009 3009 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SapI GCTCTTCn'nnn_ Cuts at: 2283 2283 Size: 5812 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2353 3160 4295 4976 5167 826 Size: 219 1308 807 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 807 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 2714 2933 3916 4817 5079 Size: 197 926 558 213 219 983 901 262 Cuts at: 5079 820 Size: 1553 Fragments arranged by size: 1553 983 926 901 558 262 219 213 197 SnaBI TAC'GTA Cuts at: 493 3109 3603 493 Size: 2616 494 2702 Fragments arranged by size: 2702 2616 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3019 3170 1132 Size: 1887 151 3774 Fragments arranged by size: 3774 1887 151 SspI AAT'ATT Cuts at: 5 52 2551 3736 5 Size: 47 2499 1185 2081 Fragments arranged by size: 2499 2081 1185 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 3251 513 Size: 552 2186 3074 Fragments arranged by size: 3074 2186 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 3839 3856 4009 4194 4639 325 Size: 1255 1302 957 17 153 185 445 1498 Fragments arranged by size: 1498 1302 1255 957 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4058 5770 96 Size: 274 80 33 51 1443 2081 1712 138 Fragments arranged by size: 2081 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI