Restriction map for
pFN21ASDA0132
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BfrI BlnI BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraI Eam1105I EarI EcoNI EcoRV
FseI FspI HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SfiI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 232 GACGTC GACGT^C
AcsI 412 GAATTC [GA]^AATT[CT]
AflIII 1724 ACACGT A^C[GA][CT]GT
AocI 70 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1604 AGCGCT AGC^GCT
ApoI 412 GAATTC [GA]^AATT[CT]
AspHI 919 GAGCTC G[AT]GC[AT]^C
AvaI 608 CCCGGG C^[CT]CG[GA]G
BbeI 960 GGCGCC GGCGC^C
BciVI 380 GTATCC GTATCC(6/5)
BclI 1496 TGATCA T^GATCA
BfaI 20 CTAG C^TAG
BglII 908 AGATCT A^GATCT
BsaWI 1058 TCCGGT [AT]^CCGG[AT]
BsiEI 266 CGGCCG CG[GA][CT]^CG
BsiHKAI 919 GAGCTC G[AT]GC[AT]^C
BsmAI 1624 GAGAC GTCTC(1/5)
BsmBI 1624 GAGACG CGTCTC(1/5)
BsoBI 608 CCCGGG C^[CT]CG[GA]G
BspDI 1261 ATCGAT AT^CGAT
BstAPI 942 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 70 CCTGAGG CC^T[ACGT]AGG
ClaI 1261 ATCGAT AT^CGAT
DraII 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 266 CGGCCG C^GGCCG
EclXI 266 CGGCCG C^GGCCG
Eco47III 1604 AGCGCT AGC^GCT
Eco52I 266 CGGCCG C^GGCCG
Eco81I 70 CCTGAGG CC^T[ACGT]AGG
EcoO109I 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 412 GAATTC G^AATTC
EcoT22I 625 ATGCAT ATGCA^T
FbaI 1496 TGATCA T^GATCA
HincII 825 GTTGAC GT[CT]^[GA]AC
HindII 825 GTTGAC GT[CT]^[GA]AC
KasI 960 GGCGCC G^GCGCC
MaeI 20 CTAG C^TAG
MseI 1451 TTAA T^TAA
NaeI 1096 GCCGGC GCC^GGC
NarI 960 GGCGCC GG^CGCC
NgoMIV 1096 GCCGGC G^CCGGC
NsiI 625 ATGCAT ATGCA^T
NspI 623 ACATGC [GA]CATG^[CT]
SacI 919 GAGCTC GAGCT^C
SbfI 1071 CCTGCAGG CCTGCA^GG
SexAI 899 ACCTGGT A^CC[AT]GGT
SfoI 960 GGCGCC GGC^GCC
SgrAI 1654 CACCGGCG C[GA]^CCGG[CT]G
SmaI 608 CCCGGG CCC^GGG
Sse8387I 1071 CCTGCAGG CCTGCA^GG
Tru9I 1451 TTAA T^TAA
XmaCI 608 CCCGGG C^CCGGG
XmaI 608 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BalI 641 TGGCCA TGG^CCA
1266 TGGCCA
BanII 919 GAGCTC G[GA]GC[CT]^C
1831 GAGCCC
BbsI 316 GTCTTC GAAGAC(2/6)
733 GTCTTC
BcgI 833 CGAACTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
545 GCAATGCCATCG
BglI 418 GCCTACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1091 GCCTGGCCGGC
BpuAI 316 GTCTTC GAAGAC(2/6)
733 GTCTTC
BseRI 652 GAGGAG GAGGAG(10/8)
916 GAGGAG
BspHI 197 TCATGA T^CATGA
464 TCATGA
BsrBI 1086 GAGCGG CCGCTC(-3/-3)
1336 GAGCGG
BsrDI 545 GCAATG GCAATG(2/0)
1504 GCAATG
BstEII 684 GGTGACC G^GT[ACGT]ACC
1779 GGTGACC
BstPI 684 GGTGACC G^GT[ACGT]ACC
1779 GGTGACC
DrdI 493 GACAGCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
895 GACTACCTGGTC
EcoO65I 684 GGTGACC G^GT[ACGT]ACC
1779 GGTGACC
KspI 989 CCGCGG CCGC^GG
1081 CCGCGG
MluNI 641 TGGCCA TGG^CCA
1266 TGGCCA
MscI 641 TGGCCA TGG^CCA
1266 TGGCCA
NcoI 437 CCATGG C^CATGG
1826 CCATGG
RcaI 197 TCATGA T^CATGA
464 TCATGA
SacII 989 CCGCGG CCGC^GG
1081 CCGCGG
StuI 16 AGGCCT AGG^CCT
185 AGGCCT
TfiI 44 GATTC G^A[AT]TC
1694 GAATC
Tsp509I 413 AATT ^AATT
1453 AATT
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BanI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraIII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII