(Circular) (Six-base) MAPSORT of: pFN21ASDA0126.seq Check: 552 from: 1 to: 7948 pFN21ASDA0126 7948 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3796 3796 Size: 7948 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5758 278 Size: 53 83 186 936 4222 2468 Fragments arranged by size: 4222 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5291 1937 Size: 3354 4594 Acc65I G'GTAC_C Cuts at: 4607 5269 4607 Size: 662 7286 AclI AA'CG_TT Cuts at: 1563 6075 6448 1563 Size: 4512 373 3063 Fragments arranged by size: 4512 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2150 3218 3392 4729 6011 7395 7663 1632 Size: 518 1068 174 1337 1282 1384 268 1917 Fragments arranged by size: 1917 1384 1337 1282 1068 518 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7751 AflIII A'CryG_T Cuts at: 1203 4162 4305 6847 7824 1203 Size: 2959 143 2542 977 1327 Fragments arranged by size: 2959 2542 1327 977 143 AgeI A'CCGG_T Cuts at: 6779 6779 Size: 7948 AhdI GACnn_n'nnGTC Cuts at: 6677 6677 Size: 7948 AleI CACnn'nnGTG Cuts at: 1166 5044 1166 Size: 3878 4070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2641 3288 1184 Size: 1457 647 5844 Fragments arranged by size: 5844 1457 647 AlwNI CAG_nnn'CTG Cuts at: 3196 5691 7263 3196 Size: 2495 1572 3881 Fragments arranged by size: 3881 2495 1572 ApaLI G'TGCA_C Cuts at: 6005 7161 6005 Size: 1156 6792 ApoI r'AATT_y Cuts at: 1493 2201 3920 4232 4366 4751 4822 5257 5483 Size: 708 1719 312 134 385 71 435 226 Cuts at: 5483 5635 7744 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1719 1697 708 435 385 312 226 152 134 71 AseI AT'TA_AT Cuts at: 160 1032 6502 160 Size: 872 5470 1606 Fragments arranged by size: 5470 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 AvaI C'yCGr_G Cuts at: 1870 1943 5273 1870 Size: 73 3330 4545 Fragments arranged by size: 4545 3330 73 BamHI G'GATC_C Cuts at: 5023 5278 5645 5023 Size: 255 367 7326 Fragments arranged by size: 7326 367 255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4607 4724 5269 6724 618 Size: 325 217 627 2820 117 545 1455 1842 Fragments arranged by size: 2820 1842 1455 627 545 325 217 117 BanII G_rGCy'C Cuts at: 1391 2743 2898 3857 5267 1391 Size: 1352 155 959 1410 4072 Fragments arranged by size: 4072 1410 1352 959 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3029 928 Size: 971 1130 5847 Fragments arranged by size: 5847 1130 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6139 6173 1903 Size: 34 4202 34 3678 Fragments arranged by size: 4202 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4150 4299 5840 7050 4150 Size: 149 1541 1210 5048 Fragments arranged by size: 5048 1541 1210 149 BclI T'GATC_A Cuts at: 1548 3622 1548 Size: 2074 5874 BfrBI ATG'CAT Cuts at: 2918 2918 Size: 7948 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6559 136 Size: 107 122 71 631 605 4887 1525 Fragments arranged by size: 4887 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2676 2760 2887 3183 7943 2676 Size: 84 127 296 4760 2681 Fragments arranged by size: 4760 2681 296 127 84 BlpI GC'TnA_GC Cuts at: 3681 4665 5365 3681 Size: 984 700 6264 Fragments arranged by size: 6264 984 700 Bme1580I G_kGCm'C Cuts at: 1790 3442 4842 6009 7165 1790 Size: 1652 1400 1167 1156 2573 Fragments arranged by size: 2573 1652 1400 1167 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5679 6637 442 Size: 416 53 83 399 24 4262 958 1753 Fragments arranged by size: 4262 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2160 1056 Size: 1104 6844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3194 3204 4025 4503 6608 1239 Size: 1955 10 821 478 2105 2579 Fragments arranged by size: 2579 2105 1955 821 478 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2038 3428 6073 6938 7236 2038 Size: 1390 2645 865 298 2750 Fragments arranged by size: 2750 2645 1390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4236 6611 882 Size: 617 2737 2375 2219 Fragments arranged by size: 2737 2375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5245 5739 493 Size: 711 4041 494 2702 Fragments arranged by size: 4041 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3740 4323 7942 3740 Size: 583 3619 3746 Fragments arranged by size: 3746 3619 583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5755 6137 6772 275 Size: 53 83 186 936 4222 382 635 1451 Fragments arranged by size: 4222 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4155 6381 6779 7053 7200 7611 4155 Size: 2226 398 274 147 411 4492 Fragments arranged by size: 4492 2226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 3524 3554 4380 Size: 30 165 30 1137 30 954 30 826 Cuts at: 4380 4410 1178 Size: 30 4716 Fragments arranged by size: 4716 1137 954 826 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3404 4832 1186 Size: 2218 1428 4302 Fragments arranged by size: 4302 2218 1428 BseYI C'CCAG_C Cuts at: 2152 2324 3031 3520 4767 7151 7647 2152 Size: 172 707 489 1247 2384 496 2453 Fragments arranged by size: 2453 2384 1247 707 496 489 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 2647 3548 4774 1163 Size: 573 906 5 901 1226 4337 Fragments arranged by size: 4337 1226 906 901 573 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5398 6159 7187 1540 Size: 275 186 3397 761 1028 2301 Fragments arranged by size: 3397 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2898 5039 6009 6094 7165 2898 Size: 2141 970 85 1071 3681 Fragments arranged by size: 3681 2141 1071 970 85 BsmI GAATG_Cn' Cuts at: 5459 5552 5459 Size: 93 7855 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2743 2898 3442 3857 4842 5039 5267 Size: 399 953 155 544 415 985 197 228 Cuts at: 5267 6009 6094 7165 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 985 953 742 544 415 399 228 197 155 85 BspEI T'CCGG_A Cuts at: 7611 7611 Size: 7948 BspHI T'CATG_A Cuts at: 2847 5837 7567 2847 Size: 2990 1730 3228 Fragments arranged by size: 3228 2990 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2613 3652 3796 4419 5303 844 Size: 1769 1039 144 623 884 3489 Fragments arranged by size: 3489 1769 1039 884 623 144 BsrBI CCG'CTC Cuts at: 1394 1823 2232 5835 1394 Size: 429 409 3603 3507 Fragments arranged by size: 3603 3507 429 409 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 3853 5097 6443 6617 65 Size: 1183 1177 1428 1244 1346 174 1396 Fragments arranged by size: 1428 1396 1346 1244 1183 1177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6592 6779 1700 Size: 110 4782 187 2869 Fragments arranged by size: 4782 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3481 96 Size: 3385 4563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7948 BssSI C'ACGA_G Cuts at: 6008 7020 6008 Size: 1012 6936 BstAPI GCAn_nnn'nTGC Cuts at: 794 3658 794 Size: 2864 5084 BstBI TT'CG_AA Cuts at: 2007 6752 2007 Size: 4745 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7948 BstYI r'GATC_y Cuts at: 1972 2292 2458 2676 2760 2887 2904 3183 3401 Size: 320 166 218 84 127 17 279 218 Cuts at: 3401 5023 5090 5128 5163 5278 5645 6030 6047 Size: 1622 67 38 35 115 367 385 17 Cuts at: 6047 7499 7585 7597 7943 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1622 1452 385 367 346 320 279 218 218 166 127 115 86 84 67 38 35 17 17 12 Bsu36I CC'TnA_GG Cuts at: 4070 4070 Size: 7948 BtgI C'CryG_G Cuts at: 513 1065 3813 5154 513 Size: 552 2748 1341 3307 Fragments arranged by size: 3307 2748 1341 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7209 Fragments arranged by size: 7209 683 56 BtsI GCAGTG_nn' Cuts at: 2625 2630 4107 5464 6258 6278 2625 Size: 5 1477 1357 794 20 4295 Fragments arranged by size: 4295 1477 1357 794 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7913 DraI TTT'AAA Cuts at: 5252 5252 Size: 7948 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6955 809 Size: 507 5639 1802 Fragments arranged by size: 5639 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3837 5168 5395 Size: 54 1425 50 275 18 2007 1331 227 Cuts at: 5395 6284 7913 8 Size: 889 1629 43 Fragments arranged by size: 2007 1629 1425 1331 889 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5395 1537 Size: 275 3583 4090 Fragments arranged by size: 4090 3583 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 2574 2787 4980 5878 1353 Size: 1088 133 213 2193 898 3423 Fragments arranged by size: 3423 2193 1088 898 213 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3950 6535 6909 7055 387 Size: 1064 138 2361 2585 374 146 1280 Fragments arranged by size: 2585 2361 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2896 2896 Size: 7948 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 3194 3204 3218 3392 4025 4503 Size: 393 518 1044 10 14 174 633 478 Cuts at: 4503 4729 6011 6608 7395 7663 1239 Size: 226 1282 597 787 268 1524 Fragments arranged by size: 1524 1282 1044 787 633 597 518 478 393 268 226 174 14 10 EcoO109I rG'GnC_Cy Cuts at: 2431 2995 2431 Size: 564 7384 EcoRI G'AATT_C Cuts at: 2201 5257 5635 2201 Size: 3056 378 4514 Fragments arranged by size: 4514 3056 378 EcoRV GAT'ATC Cuts at: 4786 4786 Size: 7948 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2423 4660 2423 Size: 2237 5711 FspI TGC'GCA Cuts at: 6454 6454 Size: 7948 HaeII r_GCGC'y Cuts at: 1678 7095 1678 Size: 5417 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3730 3762 4114 4146 4343 Size: 32 135 32 2158 32 352 32 197 Cuts at: 4343 4375 4622 4654 5097 5129 6589 6621 6663 Size: 32 247 32 443 32 1460 32 42 Cuts at: 6663 6695 1373 Size: 32 2626 Fragments arranged by size: 2626 2158 1460 443 352 247 197 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2686 5292 5538 669 Size: 988 281 748 2606 246 3079 Fragments arranged by size: 3079 2606 988 748 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7948 HpaI GTT'AAC Cuts at: 5538 5538 Size: 7948 KpnI G_GTAC'C Cuts at: 4611 5273 4611 Size: 662 7286 MfeI C'AATT_G Cuts at: 5547 5547 Size: 7948 MluI A'CGCG_T Cuts at: 7824 7824 Size: 7948 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4074 4999 5221 7062 7246 1305 Size: 2769 925 222 1841 184 2007 Fragments arranged by size: 2769 2007 1841 925 222 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3839 10 Size: 54 1425 343 2007 4119 Fragments arranged by size: 4119 2007 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3270 4679 5042 5044 5906 6265 6424 Size: 648 2104 1409 363 2 862 359 159 Cuts at: 6424 518 Size: 2042 Fragments arranged by size: 2104 2042 1409 862 648 363 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2128 3524 4567 5364 6041 7189 7434 1606 Size: 522 1396 1043 797 677 1148 245 2120 Fragments arranged by size: 2120 1396 1148 1043 797 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 3813 5154 513 Size: 552 2748 1341 3307 Fragments arranged by size: 3307 2748 1341 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7948 NheI G'CTAG_C Cuts at: 1052 2156 1052 Size: 1104 6844 NotI GC'GGCC_GC Cuts at: 5395 5395 Size: 7948 NsiI A_TGCA'T Cuts at: 2920 2920 Size: 7948 NspI r_CATG'y Cuts at: 1132 1229 3269 4013 4309 5306 6851 1132 Size: 97 2040 744 296 997 1545 2229 Fragments arranged by size: 2229 2040 1545 997 744 296 97 PacI TTA_AT'TAA Cuts at: 2478 2478 Size: 7948 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7948 PciI A'CATG_T Cuts at: 4305 6847 4305 Size: 2542 5406 PflMI CCAn_nnn'nTGG Cuts at: 1108 3291 1108 Size: 2183 5765 PfoI T'CCnGG_A Cuts at: 5019 5019 Size: 7948 PmeI GTTT'AAAC Cuts at: 5252 5252 Size: 7948 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6017 6049 7530 7562 6017 Size: 32 1481 32 6403 Fragments arranged by size: 6403 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2995 2995 Size: 7948 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7948 PsiI TTA'TAA Cuts at: 5518 5518 Size: 7948 PstI C_TGCA'G Cuts at: 830 3663 5300 830 Size: 2833 1637 3478 Fragments arranged by size: 3478 2833 1637 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7948 PvuII CAG'CTG Cuts at: 2128 3524 4567 2128 Size: 1396 1043 5509 Fragments arranged by size: 5509 1396 1043 SacI G_AGCT'C Cuts at: 2898 2898 Size: 7948 SalI G'TCGA_C Cuts at: 1936 5290 1936 Size: 3354 4594 SbfI CC_TGCA'GG Cuts at: 5300 5300 Size: 7948 ScaI AGT'ACT Cuts at: 6196 7908 6196 Size: 1712 6236 SexAI A'CCwGG_T Cuts at: 4704 4704 Size: 7948 SfcI C'TryA_G Cuts at: 826 1045 3659 3895 4550 5296 6431 7112 7303 Size: 219 2614 236 655 746 1135 681 191 Cuts at: 7303 826 Size: 1471 Fragments arranged by size: 2614 1471 1135 746 681 655 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7948 SmaI CCC'GGG Cuts at: 1872 5275 1872 Size: 3403 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 3443 6052 6953 7215 820 Size: 197 926 110 1390 2609 901 262 1553 Fragments arranged by size: 2609 1553 1390 926 901 262 197 110 SnaBI TAC'GTA Cuts at: 493 5245 5739 493 Size: 4752 494 2702 Fragments arranged by size: 4752 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7948 SphI G_CATG'C Cuts at: 1132 3269 5306 1132 Size: 2137 2037 3774 Fragments arranged by size: 3774 2137 2037 SspI AAT'ATT Cuts at: 5 52 5872 5 Size: 47 5820 2081 Fragments arranged by size: 5820 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7948 StyI C'CwwG_G Cuts at: 513 1065 2574 2991 3813 4134 4842 5154 5387 Size: 552 1509 417 822 321 708 312 233 Cuts at: 5387 513 Size: 3074 Fragments arranged by size: 3074 1509 822 708 552 417 321 312 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3347 3929 4712 5975 5992 6145 6330 Size: 1255 1767 582 783 1263 17 153 185 Cuts at: 6330 6775 325 Size: 445 1498 Fragments arranged by size: 1767 1498 1263 1255 783 582 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 3136 3481 Size: 274 80 33 51 1443 771 388 345 Cuts at: 3481 3588 3597 6194 7906 96 Size: 107 9 2597 1712 138 Fragments arranged by size: 2597 1712 1443 771 388 345 274 138 107 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3409 3441 4380 4412 7908 7940 3409 Size: 32 939 32 3496 32 3417 Fragments arranged by size: 3496 3417 939 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7948 XbaI T'CTAG_A Cuts at: 2890 5284 2890 Size: 2394 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4435 5080 4435 Size: 645 7303 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7948 XmaI C'CCGG_G Cuts at: 1870 5273 1870 Size: 3403 4545 XmnI GAAnn'nnTTC Cuts at: 2982 4691 6077 2982 Size: 1709 1386 4853 Fragments arranged by size: 4853 1709 1386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5756 276 Size: 53 83 186 936 4222 2468 Fragments arranged by size: 4222 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI