(Circular) (Six-base) MAPSORT of: pFN21ASDA0098.seq Check: 3176 from: 1 to: 6352 pFN21ASDA0098 6352 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2837 2951 3660 4415 5799 6067 1632 Size: 1205 114 709 755 1384 268 1917 Fragments arranged by size: 1917 1384 1205 755 709 268 114 AflII C'TTAA_G Cuts at: 820 1017 2254 820 Size: 197 1237 4918 Fragments arranged by size: 4918 1237 197 AflIII A'CryG_T Cuts at: 1203 2777 5251 6228 1203 Size: 1574 2474 977 1327 Fragments arranged by size: 2474 1574 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 2095 5081 2095 Size: 2986 3366 AleI CACnn'nnGTG Cuts at: 1166 2345 1166 Size: 1179 5173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 4095 5667 4095 Size: 1572 4780 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 2873 3661 3887 4039 6148 1493 Size: 1380 788 226 152 2109 1697 Fragments arranged by size: 2109 1697 1380 788 226 152 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2363 3673 5128 618 Size: 325 217 627 274 302 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 302 274 217 BanII G_rGCy'C Cuts at: 1391 2546 3066 3671 1391 Size: 1155 520 605 4072 Fragments arranged by size: 4072 1155 605 520 BbeI G_GCGC'C Cuts at: 2065 2065 Size: 6352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2426 2460 4543 4577 1903 Size: 34 489 34 2083 34 3678 Fragments arranged by size: 3678 2083 489 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 2272 3201 1548 Size: 724 929 4699 Fragments arranged by size: 4699 929 724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4963 136 Size: 107 122 71 631 605 3291 1525 Fragments arranged by size: 3291 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3105 6347 3105 Size: 3242 3110 BlpI GC'TnA_GC Cuts at: 3769 3769 Size: 6352 Bme1580I G_kGCm'C Cuts at: 1790 3313 3344 4413 5569 1790 Size: 1523 31 1069 1156 2573 Fragments arranged by size: 2573 1523 1156 1069 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4083 5041 442 Size: 416 53 83 399 24 2666 958 1753 Fragments arranged by size: 2666 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 3145 3407 3651 5012 1239 Size: 1460 446 262 244 1361 2579 Fragments arranged by size: 2579 1460 1361 446 262 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 2135 4477 5342 5640 2108 Size: 27 2342 865 298 2820 Fragments arranged by size: 2820 2342 865 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3649 4143 493 Size: 711 2445 494 2702 Fragments arranged by size: 2702 2445 711 494 BsaBI GATnn'nnATC Cuts at: 2271 3023 6346 2271 Size: 752 3323 2277 Fragments arranged by size: 3323 2277 752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2062 4159 4541 Size: 53 83 186 936 494 35 2097 382 Cuts at: 4541 5176 275 Size: 635 1451 Fragments arranged by size: 2097 1451 936 635 494 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 3249 4785 5183 5457 5604 6015 3249 Size: 1536 398 274 147 411 3586 Fragments arranged by size: 3586 1536 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2337 2367 1178 Size: 30 165 30 934 30 5163 Fragments arranged by size: 5163 934 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3201 3224 3300 1186 Size: 2015 23 76 4238 Fragments arranged by size: 4238 2015 76 23 BseYI C'CCAG_C Cuts at: 2539 2991 3079 5555 6051 2539 Size: 452 88 2476 496 2840 Fragments arranged by size: 2840 2476 496 452 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3066 4413 4498 5569 3066 Size: 1347 85 1071 3849 Fragments arranged by size: 3849 1347 1071 85 BsmI GAATG_Cn' Cuts at: 2403 2599 3863 3956 2403 Size: 196 1264 93 4799 Fragments arranged by size: 4799 1264 196 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2622 2622 Size: 6352 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2546 3066 3313 3344 3671 4413 4498 Size: 399 756 520 247 31 327 742 85 Cuts at: 4498 5569 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 756 742 520 399 327 247 85 31 BspEI T'CCGG_A Cuts at: 3249 6015 3249 Size: 2766 3586 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3707 844 Size: 2863 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2623 4239 1394 Size: 429 800 1616 3507 Fragments arranged by size: 3507 1616 800 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3097 96 Size: 3001 3351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6352 BstBI TT'CG_AA Cuts at: 2007 5156 2007 Size: 3149 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2347 2293 Size: 54 6298 BstYI r'GATC_y Cuts at: 1972 3105 3682 4049 4434 4451 5903 5989 6001 Size: 1133 577 367 385 17 1452 86 12 Cuts at: 6001 6347 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1133 577 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2031 3399 513 Size: 552 957 9 1368 3466 Fragments arranged by size: 3466 1368 957 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3010 487 Size: 683 56 1784 3829 Fragments arranged by size: 3829 1784 683 56 BtsI GCAGTG_nn' Cuts at: 3155 3868 4662 4682 3155 Size: 713 794 20 4825 Fragments arranged by size: 4825 794 713 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2730 2765 529 Size: 35 2166 35 4116 Fragments arranged by size: 4116 2166 35 35 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317 Size: 54 1425 50 275 18 1969 889 1629 Cuts at: 6317 8 Size: 43 Fragments arranged by size: 1969 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2874 4282 1353 Size: 1521 1408 3423 Fragments arranged by size: 3423 1521 1408 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3055 3347 4939 5313 5459 387 Size: 1064 138 1466 292 1592 374 146 1280 Fragments arranged by size: 1592 1466 1280 1064 374 292 146 138 EcoICRI GAG'CTC Cuts at: 3064 3064 Size: 6352 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2699 2837 2951 3145 3407 3651 3660 Size: 393 1067 138 114 194 262 244 9 Cuts at: 3660 4415 5012 5799 6067 1239 Size: 755 597 787 268 1524 Fragments arranged by size: 1524 1067 787 755 597 393 268 262 244 194 138 114 9 EcoO109I rG'GnC_Cy Cuts at: 2037 2119 3002 2037 Size: 82 883 5387 Fragments arranged by size: 5387 883 82 EcoRI G'AATT_C Cuts at: 3661 4039 3661 Size: 378 5974 EcoRV GAT'ATC Cuts at: 3303 3573 3303 Size: 270 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3100 3100 Size: 6352 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 2065 5499 1678 Size: 387 3434 2531 Fragments arranged by size: 3434 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2337 2369 2813 Size: 32 135 32 509 32 224 32 444 Cuts at: 2813 2845 3027 3059 4993 5025 5067 5099 1373 Size: 32 182 32 1934 32 42 32 2626 Fragments arranged by size: 2626 1934 509 444 224 182 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2494 2557 2635 3696 3942 669 Size: 988 281 556 63 78 1061 246 3079 Fragments arranged by size: 3079 1061 988 556 281 246 78 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 6352 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KasI G'GCGC_C Cuts at: 2061 2061 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 2387 3951 2387 Size: 1564 4788 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5466 5650 1305 Size: 4161 184 2007 Fragments arranged by size: 4161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3398 4310 4669 4828 518 Size: 648 1179 1053 912 359 159 2042 Fragments arranged by size: 2042 1179 1053 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3768 4445 5593 5838 1606 Size: 2162 677 1148 245 2120 Fragments arranged by size: 2162 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2062 2062 Size: 6352 NcoI C'CATG_G Cuts at: 513 1065 2022 2031 3399 513 Size: 552 957 9 1368 3466 Fragments arranged by size: 3466 1368 957 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2781 3539 3710 5255 1132 Size: 97 1552 758 171 1545 2229 Fragments arranged by size: 2229 1552 1545 758 171 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 2777 5251 2777 Size: 2474 3878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2037 3002 2037 Size: 965 5387 PshAI GACnn'nnGTC Cuts at: 1613 2831 3449 1613 Size: 1218 618 4516 Fragments arranged by size: 4516 1218 618 PsiI TTA'TAA Cuts at: 2844 3922 2844 Size: 1078 5274 PstI C_TGCA'G Cuts at: 830 3704 830 Size: 2874 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SacI G_AGCT'C Cuts at: 3066 3066 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SanDI GG'GwC_CC Cuts at: 2037 2037 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 2151 2607 3700 4835 5516 5707 826 Size: 219 1106 456 1093 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 1093 681 456 219 191 SfoI GGC'GCC Cuts at: 2063 2063 Size: 6352 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 2123 2254 4456 5357 5619 Size: 197 926 171 9 131 2202 901 262 Cuts at: 5619 820 Size: 1553 Fragments arranged by size: 2202 1553 926 901 262 197 171 131 9 SnaBI TAC'GTA Cuts at: 493 3649 4143 493 Size: 3156 494 2702 Fragments arranged by size: 3156 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6352 StyI C'CwwG_G Cuts at: 513 1065 2022 2031 3399 3791 513 Size: 552 957 9 1368 392 3074 Fragments arranged by size: 3074 1368 957 552 392 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2412 2424 2745 2809 3461 4379 4396 Size: 1255 832 12 321 64 652 918 17 Cuts at: 4396 4549 4734 5179 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 918 832 652 445 321 185 153 64 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3097 4598 6310 Size: 274 80 33 51 1443 1120 1501 1712 Cuts at: 6310 96 Size: 138 Fragments arranged by size: 1712 1501 1443 1120 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6312 6344 6312 Size: 32 6320 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2293 2029 Size: 264 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI