Restriction map for
pFN21ASDA0096
(Linear) (Six-base) MAPSORT of: KIAA0096.seq Check: 598 from: 1 to: 2295
KIAA0096.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 588 2295
Size: 588 1707
Acc65I G'GTAC_C
Cuts at: 0 1861 2295
Size: 1861 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 2295
Size: 790 1505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1912 2111 2295
Size: 1912 199 184
Fragments arranged by size:
1912 199 184
ApaI G_GGCC'C
Cuts at: 0 1718 2295
Size: 1718 577
ApoI r'AATT_y
Cuts at: 0 490 551 2295
Size: 490 61 1744
Fragments arranged by size:
1744 490 61
AvaI C'yCGr_G
Cuts at: 0 1053 1175 1833 2295
Size: 1053 122 658 462
Fragments arranged by size:
1053 658 462 122
BamHI G'GATC_C
Cuts at: 0 1004 2295
Size: 1004 1291
BanI G'GyrC_C
Cuts at: 0 1278 1691 1861 2295
Size: 1278 413 170 434
Fragments arranged by size:
1278 434 413 170
BanII G_rGCy'C
Cuts at: 0 1606 1718 1928 2295
Size: 1606 112 210 367
Fragments arranged by size:
1606 367 210 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 429 2295
Size: 344 85 1866
Fragments arranged by size:
1866 344 85
BclI T'GATC_A
Cuts at: 0 677 2295
Size: 677 1618
BfrBI ATG'CAT
Cuts at: 0 197 2295
Size: 197 2098
BglII A'GATC_T
Cuts at: 0 1042 1463 2295
Size: 1042 421 832
Fragments arranged by size:
1042 832 421
Bme1580I G_kGCm'C
Cuts at: 0 1718 2295
Size: 1718 577
BmrI ACTGGGnnnn_n'
Cuts at: 0 1065 1787 2295
Size: 1065 722 508
Fragments arranged by size:
1065 722 508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1538 1590 1809 2295
Size: 530 1008 52 219 486
Fragments arranged by size:
1008 530 486 219 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 2295
Size: 1138 1157
BsaI GGTCTCn'nnnn_
Cuts at: 0 1618 2295
Size: 1618 677
BsaAI yAC'GTr
Cuts at: 0 2051 2295
Size: 2051 244
BsaBI GATnn'nnATC
Cuts at: 0 1508 2295
Size: 1508 787
BsaHI Gr'CG_yC
Cuts at: 0 932 2295
Size: 932 1363
BsaWI w'CCGG_w
Cuts at: 0 1580 2295
Size: 1580 715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1122 1152 1575 1605 2295
Size: 1122 30 423 30 690
Fragments arranged by size:
1122 690 423 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 305 337 892 1372 2295
Size: 305 32 555 480 923
Fragments arranged by size:
923 555 480 305 32
BseYI C'CCAG_C
Cuts at: 0 1885 1918 1967 2271 2295
Size: 1885 33 49 304 24
Fragments arranged by size:
1885 304 49 33 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 2295
Size: 229 2066
BsiHKAI G_wGCw'C
Cuts at: 0 884 1928 1984 2295
Size: 884 1044 56 311
Fragments arranged by size:
1044 884 311 56
BsmI GAATG_Cn'
Cuts at: 0 2085 2295
Size: 2085 210
BsmBI CGTCTCn'nnnn_
Cuts at: 0 923 2295
Size: 923 1372
Bsp1286I G_dGCh'C
Cuts at: 0 884 1606 1718 1928 1984 2295
Size: 884 722 112 210 56 311
Fragments arranged by size:
884 722 311 210 112 56
BspHI T'CATG_A
Cuts at: 0 1946 2295
Size: 1946 349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 1520 2079 2295
Size: 588 932 559 216
Fragments arranged by size:
932 588 559 216
BsrBI CCG'CTC
Cuts at: 0 544 2295
Size: 544 1751
BsrDI GCAATG_nn'
Cuts at: 0 1767 2295
Size: 1767 528
BsrFI r'CCGG_y
Cuts at: 0 1649 1879 2295
Size: 1649 230 416
Fragments arranged by size:
1649 416 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1902 1975 2151 2295
Size: 1902 73 176 144
Fragments arranged by size:
1902 176 144 73
BstYI r'GATC_y
Cuts at: 0 526 756 1004 1042 1463 2295
Size: 526 230 248 38 421 832
Fragments arranged by size:
832 526 421 248 230 38
Bsu36I CC'TnA_GG
Cuts at: 0 2243 2295
Size: 2243 52
BtgI C'CryG_G
Cuts at: 0 83 2295
Size: 83 2212
BtsI GCAGTG_nn'
Cuts at: 0 1731 1920 2105 2295
Size: 1731 189 185 190
Fragments arranged by size:
1731 190 189 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1373 1408 1749 1784 1791 1826 2295
Size: 1373 35 341 35 7 35 469
Fragments arranged by size:
1373 469 341 35 35 35 7
EaeI y'GGCC_r
Cuts at: 0 344 1087 1133 1200 2295
Size: 344 743 46 67 1095
Fragments arranged by size:
1095 743 344 67 46
EarI CTCTTCn'nnn_
Cuts at: 0 776 869 1340 1435 2295
Size: 776 93 471 95 860
Fragments arranged by size:
860 776 471 95 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 1329 2295
Size: 217 1112 966
Fragments arranged by size:
1112 966 217
EcoICRI GAG'CTC
Cuts at: 0 1926 2295
Size: 1926 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 790 1538 1590 1809 2295
Size: 530 260 748 52 219 486
Fragments arranged by size:
748 530 486 260 219 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1113 1714 1715 2091 2295
Size: 1113 601 1 376 204
Fragments arranged by size:
1113 601 376 204 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 791 2295
Size: 791 1504
FspI TGC'GCA
Cuts at: 0 2262 2295
Size: 2262 33
FspAI rTGC'GCAy
Cuts at: 0 2262 2295
Size: 2262 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 1146 1178 1253 1285 1575 1607
Size: 777 32 337 32 75 32 290 32
Cuts at: 1607 2295
Size: 688
Fragments arranged by size:
777 688 337 290 75 32 32 32
32
HincII GTy'rAC
Cuts at: 0 467 2295
Size: 467 1828
HindIII A'AGCT_T
Cuts at: 0 1290 2295
Size: 1290 1005
KpnI G_GTAC'C
Cuts at: 0 1865 2295
Size: 1865 430
MscI TGG'CCA
Cuts at: 0 346 1089 1202 2295
Size: 346 743 113 1093
Fragments arranged by size:
1093 743 346 113
MslI CAynn'nnrTG
Cuts at: 0 622 742 2221 2295
Size: 622 120 1479 74
Fragments arranged by size:
1479 622 120 74
MspA1I CmG'CkG
Cuts at: 0 1610 1829 1874 1902 2235 2279 2295
Size: 1610 219 45 28 333 44 16
Fragments arranged by size:
1610 333 219 45 44 28 16
NaeI GCC'GGC
Cuts at: 0 1651 1881 2295
Size: 1651 230 414
Fragments arranged by size:
1651 414 230
NgoMIV G'CCGG_C
Cuts at: 0 1649 1879 2295
Size: 1649 230 416
Fragments arranged by size:
1649 416 230
NsiI A_TGCA'T
Cuts at: 0 199 2295
Size: 199 2096
NspI r_CATG'y
Cuts at: 0 106 2295
Size: 106 2189
PflMI CCAn_nnn'nTGG
Cuts at: 0 2008 2295
Size: 2008 287
PfoI T'CCnGG_A
Cuts at: 0 182 1435 2295
Size: 182 1253 860
Fragments arranged by size:
1253 860 182
PpuMI rG'GwC_Cy
Cuts at: 0 1113 2091 2295
Size: 1113 978 204
Fragments arranged by size:
1113 978 204
PspOMI G'GGCC_C
Cuts at: 0 1714 2295
Size: 1714 581
PstI C_TGCA'G
Cuts at: 0 585 1615 2100 2195 2295
Size: 585 1030 485 95 100
Fragments arranged by size:
1030 585 485 100 95
PvuII CAG'CTG
Cuts at: 0 1610 1902 2279 2295
Size: 1610 292 377 16
Fragments arranged by size:
1610 377 292 16
SacI G_AGCT'C
Cuts at: 0 1928 2295
Size: 1928 367
SfcI C'TryA_G
Cuts at: 0 581 748 1611 2096 2191 2295
Size: 581 167 863 485 95 104
Fragments arranged by size:
863 581 485 167 104 95
SmlI C'TyrA_G
Cuts at: 0 1117 2295
Size: 1117 1178
SpeI A'CTAG_T
Cuts at: 0 204 2295
Size: 204 2091
StuI AGG'CCT
Cuts at: 0 1217 2295
Size: 1217 1078
StyI C'CwwG_G
Cuts at: 0 62 799 2295
Size: 62 737 1496
Fragments arranged by size:
1496 737 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 2295
Size: 265 2030
TatI w'GTAC_w
Cuts at: 0 1988 2295
Size: 1988 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 2295
Size: 1122 32 1141
Fragments arranged by size:
1141 1122 32
Tth111I GACn'n_nGTC
Cuts at: 0 1189 2295
Size: 1189 1106
XbaI T'CTAG_A
Cuts at: 0 273 2295
Size: 273 2022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1773 2148 2295
Size: 1773 375 147
Fragments arranged by size:
1773 375 147
XmnI GAAnn'nnTTC
Cuts at: 0 771 2295
Size: 771 1524
Enzymes that do cut:
AarI Acc65I AcuI AlfI ApaI ApoI AvaI BamHI
BanI BanII BbsI BclI BfrBI BglII Bme1580I BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI FspI FspAI Hin4I HincII HindIII KpnI MscI
MslI MspA1I NaeI NgoMIV NsiI NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SfcI SmlI SpeI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI
BmtI BplI Bpu10I BsiEI BsiWI BspEI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FseI HaeII
HpaI KasI MfeI MluI MmeI NarI NcoI NdeI
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
XhoI XmaI ZraI