(Circular) (Six-base) MAPSORT of: pFN21ASDA0083.seq Check: 7266 from: 1 to: 7909 pFN21ASDA0083 7909 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3554 3554 Size: 7909 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5020 5719 278 Size: 53 83 186 936 3484 699 2468 Fragments arranged by size: 3484 2468 936 699 186 83 53 AccI GT'mk_AC Cuts at: 1937 4920 5252 1937 Size: 2983 332 4594 Fragments arranged by size: 4594 2983 332 Acc65I G'GTAC_C Cuts at: 2160 5230 2160 Size: 3070 4839 AclI AA'CG_TT Cuts at: 1563 6036 6409 1563 Size: 4473 373 3063 Fragments arranged by size: 4473 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3341 5972 7356 7624 1632 Size: 1709 2631 1384 268 1917 Fragments arranged by size: 2631 1917 1709 1384 268 AflII C'TTAA_G Cuts at: 820 1017 4528 820 Size: 197 3511 4201 Fragments arranged by size: 4201 3511 197 AflIII A'CryG_T Cuts at: 1203 2771 3481 3774 4247 6808 7785 1203 Size: 1568 710 293 473 2561 977 1327 Fragments arranged by size: 2561 1568 1327 977 710 473 293 AgeI A'CCGG_T Cuts at: 2157 6740 2157 Size: 4583 3326 AhdI GACnn_n'nnGTC Cuts at: 6638 6638 Size: 7909 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7909 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7909 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2247 Size: 32 7877 AlwNI CAG_nnn'CTG Cuts at: 3011 3319 5652 7224 3011 Size: 308 2333 1572 3696 Fragments arranged by size: 3696 2333 1572 308 ApaI G_GGCC'C Cuts at: 4346 4346 Size: 7909 ApaLI G'TGCA_C Cuts at: 5966 7122 5966 Size: 1156 6753 ApoI r'AATT_y Cuts at: 1493 2938 4011 4170 4284 4634 5218 5444 5596 Size: 1445 1073 159 114 350 584 226 152 Cuts at: 5596 7705 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1445 1073 584 350 226 159 152 114 AseI AT'TA_AT Cuts at: 160 1032 5135 6463 160 Size: 872 4103 1328 1606 Fragments arranged by size: 4103 1606 1328 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 AvaI C'yCGr_G Cuts at: 1870 1943 5234 1870 Size: 73 3291 4545 Fragments arranged by size: 4545 3291 73 AvrII C'CTAG_G Cuts at: 2825 2825 Size: 7909 BamHI G'GATC_C Cuts at: 5239 5606 5239 Size: 367 7542 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2160 2402 3536 5230 6685 Size: 325 217 627 373 242 1134 1694 1455 Cuts at: 6685 618 Size: 1842 Fragments arranged by size: 1842 1694 1455 1134 627 373 325 242 217 BanII G_rGCy'C Cuts at: 1391 2468 4346 4429 5228 1391 Size: 1077 1878 83 799 4072 Fragments arranged by size: 4072 1878 1077 799 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3158 928 Size: 971 1259 5679 Fragments arranged by size: 5679 1259 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6100 6134 1903 Size: 34 4163 34 3678 Fragments arranged by size: 4163 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2391 3865 5801 7011 2391 Size: 1474 1936 1210 3289 Fragments arranged by size: 3289 1936 1474 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7909 BfrBI ATG'CAT Cuts at: 2262 2698 2262 Size: 436 7473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3913 6520 136 Size: 107 122 71 631 605 2241 2607 1525 Fragments arranged by size: 2607 2241 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7904 7904 Size: 7909 BlpI GC'TnA_GC Cuts at: 5326 5326 Size: 7909 Bme1580I G_kGCm'C Cuts at: 1790 4346 5076 5970 7126 1790 Size: 2556 730 894 1156 2573 Fragments arranged by size: 2573 2556 1156 894 730 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5192 5640 6598 Size: 416 53 83 399 24 3775 448 958 Cuts at: 6598 442 Size: 1753 Fragments arranged by size: 3775 1753 958 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 3392 4481 4580 6569 1239 Size: 824 1329 1089 99 1989 2579 Fragments arranged by size: 2579 1989 1329 1089 824 99 Bpu10I CC'TnA_GC Cuts at: 4198 4198 Size: 7909 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3014 6034 6899 7197 3014 Size: 3020 865 298 3726 Fragments arranged by size: 3726 3020 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6572 882 Size: 617 5073 2219 Fragments arranged by size: 5073 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5206 5700 493 Size: 711 4002 494 2702 Fragments arranged by size: 4002 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7903 7903 Size: 7909 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5017 5716 6098 6733 Size: 53 83 186 936 3484 699 382 635 Cuts at: 6733 275 Size: 1451 Fragments arranged by size: 3484 1451 936 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2157 6342 6740 7014 7161 7572 2157 Size: 4185 398 274 147 411 2494 Fragments arranged by size: 4185 2494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7684 Fragments arranged by size: 7684 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 1186 Size: 901 7008 BseYI C'CCAG_C Cuts at: 3312 7112 7608 3312 Size: 3800 496 3613 Fragments arranged by size: 3800 3613 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2314 4517 1163 Size: 573 578 2203 4555 Fragments arranged by size: 4555 2203 578 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5359 6120 7148 1540 Size: 275 186 3358 761 1028 2301 Fragments arranged by size: 3358 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 2468 3232 4429 5970 6055 7126 2144 Size: 324 764 1197 1541 85 1071 2927 Fragments arranged by size: 2927 1541 1197 1071 764 324 85 BsmI GAATG_Cn' Cuts at: 3107 3885 5420 5513 3107 Size: 778 1535 93 5503 Fragments arranged by size: 5503 1535 778 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2468 3232 4346 4429 5076 5228 Size: 399 354 324 764 1114 83 647 152 Cuts at: 5228 5970 6055 7126 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1114 1071 764 742 647 399 354 324 152 85 83 BspEI T'CCGG_A Cuts at: 7572 7572 Size: 7909 BspHI T'CATG_A Cuts at: 5798 7528 5798 Size: 1730 6179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 5264 844 Size: 2710 1710 3489 Fragments arranged by size: 3489 2710 1710 BsrBI CCG'CTC Cuts at: 1394 1823 5796 1394 Size: 429 3973 3507 Fragments arranged by size: 3973 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4765 6404 6578 65 Size: 1183 3517 1639 174 1396 Fragments arranged by size: 3517 1639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 2157 6553 6740 1700 Size: 110 276 71 4396 187 2869 Fragments arranged by size: 4396 2869 276 187 110 71 BsrGI T'GTAC_A Cuts at: 96 2185 2970 4483 96 Size: 2089 785 1513 3522 Fragments arranged by size: 3522 2089 1513 785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7909 BssSI C'ACGA_G Cuts at: 5969 6981 5969 Size: 1012 6897 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7909 BstBI TT'CG_AA Cuts at: 2007 6713 2007 Size: 4706 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7909 BstXI CCAn_nnnn'nTGG Cuts at: 3766 3766 Size: 7909 BstYI r'GATC_y Cuts at: 1972 5239 5606 5991 6008 7460 7546 7558 7904 Size: 3267 367 385 17 1452 86 12 346 Cuts at: 7904 1972 Size: 1977 Fragments arranged by size: 3267 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2267 2267 Size: 7909 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6400 Fragments arranged by size: 6400 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3935 487 Size: 683 56 2709 4461 Fragments arranged by size: 4461 2709 683 56 BtsI GCAGTG_nn' Cuts at: 2226 4596 5425 6219 6239 2226 Size: 2370 829 794 20 3896 Fragments arranged by size: 3896 2370 829 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7909 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7874 DraI TTT'AAA Cuts at: 4099 4612 5121 5213 4099 Size: 513 509 92 6795 Fragments arranged by size: 6795 513 509 92 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6916 809 Size: 507 5600 1802 Fragments arranged by size: 5600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4581 5356 6245 Size: 54 1425 50 275 18 2751 775 889 Cuts at: 6245 7874 8 Size: 1629 43 Fragments arranged by size: 2751 1629 1425 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5356 1537 Size: 275 3544 4090 Fragments arranged by size: 4090 3544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2431 2989 3183 3437 4209 5839 1353 Size: 701 377 558 194 254 772 1630 3423 Fragments arranged by size: 3423 1630 772 701 558 377 254 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 2105 6496 6870 7016 387 Size: 1064 138 504 12 4391 374 146 1280 Fragments arranged by size: 4391 1280 1064 504 374 146 138 12 EcoICRI GAG'CTC Cuts at: 2466 4427 2466 Size: 1961 5948 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 3341 3392 4481 4580 5972 6569 Size: 393 431 1278 51 1089 99 1392 597 Cuts at: 6569 7356 7624 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1392 1278 1089 787 597 431 393 268 99 51 EcoNI CCTnn'n_nnAGG Cuts at: 3975 3975 Size: 7909 EcoO109I rG'GnC_Cy Cuts at: 4343 4343 Size: 7909 EcoRI G'AATT_C Cuts at: 4011 5218 5596 4011 Size: 1207 378 6324 Fragments arranged by size: 6324 1207 378 EcoRV GAT'ATC Cuts at: 2308 2308 Size: 7909 FspI TGC'GCA Cuts at: 6415 6415 Size: 7909 HaeII r_GCGC'y Cuts at: 1678 2218 4730 7056 1678 Size: 540 2512 2326 2531 Fragments arranged by size: 2531 2512 2326 540 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 2365 2397 2455 Size: 32 135 32 746 32 15 32 58 Cuts at: 2455 2487 3365 3397 3985 4017 4298 4330 4553 Size: 32 878 32 588 32 281 32 223 Cuts at: 4553 4585 5135 5167 6550 6582 6624 6656 1373 Size: 32 550 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 878 746 588 550 281 223 135 58 42 32 32 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3366 4044 4069 4229 4491 5253 Size: 988 281 1428 678 25 160 262 762 Cuts at: 5253 5499 669 Size: 246 3079 Fragments arranged by size: 3079 1428 988 762 678 281 262 246 160 25 HindIII A'AGCT_T Cuts at: 748 4444 748 Size: 3696 4213 HpaI GTT'AAC Cuts at: 3366 4491 5499 3366 Size: 1125 1008 5776 Fragments arranged by size: 5776 1125 1008 KpnI G_GTAC'C Cuts at: 2164 5234 2164 Size: 3070 4839 MfeI C'AATT_G Cuts at: 3223 3279 5508 3223 Size: 56 2229 5624 Fragments arranged by size: 5624 2229 56 MluI A'CGCG_T Cuts at: 7785 7785 Size: 7909 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7023 7207 1305 Size: 5718 184 2007 Fragments arranged by size: 5718 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4583 10 Size: 54 1425 343 2751 3336 Fragments arranged by size: 3336 2751 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 5867 6226 6385 518 Size: 648 981 3720 359 159 2042 Fragments arranged by size: 3720 2042 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2911 5325 6002 7150 7395 1606 Size: 426 879 2414 677 1148 245 2120 Fragments arranged by size: 2414 2120 1148 879 677 426 245 NaeI GCC'GGC Cuts at: 2088 2088 Size: 7909 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6400 Fragments arranged by size: 6400 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7909 NgoMIV G'CCGG_C Cuts at: 2086 2086 Size: 7909 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7909 NotI GC'GGCC_GC Cuts at: 5356 5356 Size: 7909 NsiI A_TGCA'T Cuts at: 2264 2700 2264 Size: 436 7473 NspI r_CATG'y Cuts at: 1132 1229 2392 2775 3485 4251 5267 6812 1132 Size: 97 1163 383 710 766 1016 1545 2229 Fragments arranged by size: 2229 1545 1163 1016 766 710 383 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7909 PciI A'CATG_T Cuts at: 2771 3481 4247 6808 2771 Size: 710 766 2561 3872 Fragments arranged by size: 3872 2561 766 710 PflMI CCAn_nnn'nTGG Cuts at: 1108 3011 5182 1108 Size: 1903 2171 3835 Fragments arranged by size: 3835 2171 1903 PmeI GTTT'AAAC Cuts at: 5213 5213 Size: 7909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5978 6010 7491 7523 5978 Size: 32 1481 32 6364 Fragments arranged by size: 6364 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7909 PsiI TTA'TAA Cuts at: 5479 5479 Size: 7909 PspOMI G'GGCC_C Cuts at: 4342 4342 Size: 7909 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3193 3225 3193 Size: 32 7877 PstI C_TGCA'G Cuts at: 830 5261 830 Size: 4431 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7909 PvuII CAG'CTG Cuts at: 2032 2032 Size: 7909 SacI G_AGCT'C Cuts at: 2468 4429 2468 Size: 1961 5948 SalI G'TCGA_C Cuts at: 1936 5251 1936 Size: 3315 4594 SapI GCTCTTCn'nnn_ Cuts at: 2431 2989 4209 2431 Size: 558 1220 6131 Fragments arranged by size: 6131 1220 558 SbfI CC_TGCA'GG Cuts at: 5261 5261 Size: 7909 ScaI AGT'ACT Cuts at: 6157 7869 6157 Size: 1712 6197 SexAI A'CCwGG_T Cuts at: 2163 2163 Size: 7909 SfcI C'TryA_G Cuts at: 826 1045 5257 6392 7073 7264 826 Size: 219 4212 1135 681 191 1471 Fragments arranged by size: 4212 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7909 SmaI CCC'GGG Cuts at: 1872 5236 1872 Size: 3364 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3029 4528 6013 6914 7176 820 Size: 197 926 1086 1499 1485 901 262 1553 Fragments arranged by size: 1553 1499 1485 1086 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5206 5700 493 Size: 4713 494 2702 Fragments arranged by size: 4713 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7909 SphI G_CATG'C Cuts at: 1132 5267 1132 Size: 4135 3774 SspI AAT'ATT Cuts at: 5 52 3206 3341 3509 4075 4272 5833 5 Size: 47 3154 135 168 566 197 1561 2081 Fragments arranged by size: 3154 2081 1561 566 197 168 135 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7909 StyI C'CwwG_G Cuts at: 513 1065 2022 2825 4661 4930 5348 513 Size: 552 957 803 1836 269 418 3074 Fragments arranged by size: 3074 1836 957 803 552 418 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3667 5174 5936 5953 6106 6291 6736 Size: 1255 2087 1507 762 17 153 185 445 Cuts at: 6736 325 Size: 1498 Fragments arranged by size: 2087 1507 1498 1255 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2185 2970 3998 Size: 274 80 33 51 1443 208 785 1028 Cuts at: 3998 4483 4500 6155 7867 96 Size: 485 17 1655 1712 138 Fragments arranged by size: 1712 1655 1443 1028 785 485 274 208 138 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7869 7901 7869 Size: 32 7877 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7909 XbaI T'CTAG_A Cuts at: 3067 3614 4207 5245 3067 Size: 547 593 1038 5731 Fragments arranged by size: 5731 1038 593 547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2492 3530 2492 Size: 1038 6871 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7909 XmaI C'CCGG_G Cuts at: 1870 5234 1870 Size: 3364 4545 XmnI GAAnn'nnTTC Cuts at: 3754 6038 3754 Size: 2284 5625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5018 5717 276 Size: 53 83 186 936 3484 699 2468 Fragments arranged by size: 3484 2468 936 699 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI