Restriction map for
pFN21ASDA0081
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 436 GACGTC GACGT^C
AfaI 567 GTAC GT^AC
AlwI 457 GGATC GGATC(4/5)
AlwNI 263 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 247 GAGCAC G[AT]GC[AT]^C
AvaI 112 CCCGGG C^[CT]CG[GA]G
BmyI 247 GAGCAC G[AGT]GC[ACT]^C
BsaI 255 GAGACC GGTCTC(1/5)
BseRI 581 GAGGAG GAGGAG(10/8)
BsiEI 98 CGGCCG CG[GA][CT]^CG
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BsmFI 115 GGGAC GGGAC(10/14)
BsoBI 112 CCCGGG C^[CT]CG[GA]G
Bsp1286I 247 GAGCAC G[AGT]GC[ACT]^C
BspHI 341 TCATGA T^CATGA
BspMI 53 ACCTGC ACCTGC(4/8)
BsrDI 204 GCAATG GCAATG(2/0)
BssHII 19 GCGCGC G^CGCGC
DdeI 374 CTGAG C^T[ACGT]AG
DraII 88 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 514 GACTTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 29 CCGTGG C^C[GA][CT]GG
EaeI 98 CGGCCG [CT]^GGCC[GA]
EagI 98 CGGCCG C^GGCCG
EclXI 98 CGGCCG C^GGCCG
Eco52I 98 CGGCCG C^GGCCG
EcoNI 402 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 88 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 662 CCAAGG C^C[AT][AT]GG
HincII 271 GTCGAC GT[CT]^[GA]AC
HindII 271 GTCGAC GT[CT]^[GA]AC
MaeII 437 ACGT A^CGT
NlaIV 88 GGGTCC GG[ACGT]^[ACGT]CC
PflMI 79 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 88 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 341 TCATGA T^CATGA
RsaI 567 GTAC GT^AC
SalI 271 GTCGAC G^TCGAC
SfaNI 178 GATGC GCATC(5/9)
SmaI 112 CCCGGG CCC^GGG
StyI 662 CCAAGG C^C[AT][AT]GG
Tsp45I 352 GTCAC ^GT[GC]AC
Tsp509I 393 AATT ^AATT
TspRI 354 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 79 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 129 GTCTAC GT^[AC][GT]AC
271 GTCGAC
AcyI 117 GACGCC G[GA]^CG[CT]C
436 GACGTC
AluI 493 AGCT AG^CT
678 AGCT
AvaII 33 GGTCC G^G[AT]CC
89 GGTCC
BglII 237 AGATCT A^GATCT
642 AGATCT
BpmI 199 CTGGAG CTGGAG(16/14)
553 CTGGAG
BsaHI 117 GACGCC G[GA]^CG[CT]C
436 GACGTC
BsmAI 255 GAGAC GTCTC(1/5)
382 GAGAC
BstYI 237 AGATCT [GA]^GATC[CT]
642 AGATCT
HgaI 117 GACGC GACGC(5/10)
192 GCGTC
Hin1I 117 GACGCC G[GA]^CG[CT]C
436 GACGTC
HinfI 127 GAGTC G^A[ACGT]TC
336 GACTC
MaeIII 352 GTCAC ^GT[ACGT]AC
547 GTAAC
MflI 237 AGATCT [GA]^GATC[CT]
642 AGATCT
NruI 165 TCGCGA TCG^CGA
483 TCGCGA
PleI 336 GACTC GAGTC(4/5)
127 GAGTC
TaqI 272 TCGA T^CGA
675 TCGA
TthHB8I 272 TCGA T^CGA
675 TCGA
XhoII 237 AGATCT [GA]^GATC[CT]
642 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI BbsI BbvI BcnI
BpuAI BsaJI BsiYI BslI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII Sau3AI
Sau96I ScrFI TseI