(Circular) (Six-base) MAPSORT of: pFN21ASDA0070.seq Check: 5832 from: 1 to: 6520 pFN21ASDA0070 6520 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2373 3575 2373 Size: 1202 5318 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 3518 3813 3863 1937 Size: 388 1193 295 50 4594 Fragments arranged by size: 4594 1193 388 295 50 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2303 3291 4583 5967 6235 1632 Size: 479 192 988 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 988 479 268 192 AfeI AGC'GCT Cuts at: 3476 3476 Size: 6520 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 5419 6396 1203 Size: 4216 977 1327 Fragments arranged by size: 4216 1327 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AlwNI CAG_nnn'CTG Cuts at: 4263 5835 4263 Size: 1572 4948 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2525 2750 3285 3829 4055 4207 6316 1493 Size: 1032 225 535 544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 544 535 226 225 152 AseI AT'TA_AT Cuts at: 160 1032 2538 5074 160 Size: 872 1506 2536 1606 Fragments arranged by size: 2536 1606 1506 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 3845 1870 Size: 73 1902 4545 Fragments arranged by size: 4545 1902 73 BamHI G'GATC_C Cuts at: 2415 3850 4217 2415 Size: 1435 367 4718 Fragments arranged by size: 4718 1435 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3447 3841 5296 618 Size: 325 217 627 1660 394 1455 1842 Fragments arranged by size: 1842 1660 1455 627 394 325 217 BanII G_rGCy'C Cuts at: 1391 2151 3434 3839 1391 Size: 760 1283 405 4072 Fragments arranged by size: 4072 1283 760 405 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2304 3756 928 Size: 971 405 1452 3692 Fragments arranged by size: 3692 1452 971 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 3412 1548 Size: 1864 4656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3159 5131 Size: 107 122 71 631 605 364 1123 1972 Cuts at: 5131 136 Size: 1525 Fragments arranged by size: 1972 1525 1123 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6515 6515 Size: 6520 BlpI GC'TnA_GC Cuts at: 2165 2609 3937 2165 Size: 444 1328 4748 Fragments arranged by size: 4748 1328 444 Bme1580I G_kGCm'C Cuts at: 1790 4581 5737 1790 Size: 2791 1156 2573 Fragments arranged by size: 2791 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4251 5209 442 Size: 416 53 83 399 24 2834 958 1753 Fragments arranged by size: 2834 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2934 2934 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2904 5180 1239 Size: 1665 2276 2579 Fragments arranged by size: 2579 2276 1665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3242 4645 5510 5808 3242 Size: 1403 865 298 3954 Fragments arranged by size: 3954 1403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5183 882 Size: 617 3684 2219 Fragments arranged by size: 3684 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3817 4311 493 Size: 711 2613 494 2702 Fragments arranged by size: 2702 2613 711 494 BsaBI GATnn'nnATC Cuts at: 2837 6514 2837 Size: 3677 2843 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 2980 4327 4709 Size: 53 83 186 936 565 882 1347 382 Cuts at: 4709 5344 275 Size: 635 1451 Fragments arranged by size: 1451 1347 936 882 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2988 4953 5351 5625 5772 6183 2988 Size: 1965 398 274 147 411 3325 Fragments arranged by size: 3325 1965 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2460 2490 1178 Size: 30 165 30 1057 30 5208 Fragments arranged by size: 5208 1057 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2481 1186 Size: 1295 5225 BseYI C'CCAG_C Cuts at: 2803 3660 5723 6219 2803 Size: 857 2063 496 3104 Fragments arranged by size: 3104 2063 857 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 1163 Size: 573 318 5629 Fragments arranged by size: 5629 573 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2043 2962 3970 4731 5759 Size: 275 186 30 12 919 1008 761 1028 Cuts at: 5759 1540 Size: 2301 Fragments arranged by size: 2301 1028 1008 919 761 275 186 30 12 BsiHKAI G_wGCw'C Cuts at: 2151 4581 4666 5737 2151 Size: 2430 85 1071 2934 Fragments arranged by size: 2934 2430 1071 85 BsmI GAATG_Cn' Cuts at: 3326 4031 4124 3326 Size: 705 93 5722 Fragments arranged by size: 5722 705 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2089 2089 Size: 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 3434 3839 4581 4666 5737 1391 Size: 399 361 1283 405 742 85 1071 2174 Fragments arranged by size: 2174 1283 1071 742 405 399 361 85 BspEI T'CCGG_A Cuts at: 2988 6183 2988 Size: 3195 3325 BspHI T'CATG_A Cuts at: 3492 4409 6139 3492 Size: 917 1730 3873 Fragments arranged by size: 3873 1730 917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 2399 3575 3875 844 Size: 1529 26 1176 300 3489 Fragments arranged by size: 3489 1529 1176 300 26 BsrBI CCG'CTC Cuts at: 1394 1823 2763 3453 4407 1394 Size: 429 940 690 954 3507 Fragments arranged by size: 3507 954 940 690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3517 5015 5189 65 Size: 1183 2269 1498 174 1396 Fragments arranged by size: 2269 1498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5164 5351 1700 Size: 110 3354 187 2869 Fragments arranged by size: 3354 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 2007 5324 2007 Size: 3317 3203 BstEII G'GTnAC_C Cuts at: 1184 3133 1184 Size: 1949 4571 BstXI CCAn_nnnn'nTGG Cuts at: 3149 3149 Size: 6520 BstYI r'GATC_y Cuts at: 1972 2415 3850 4217 4602 4619 6071 6157 6169 Size: 443 1435 367 385 17 1452 86 12 Cuts at: 6169 6515 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1435 443 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3519 3519 Size: 6520 Bsu36I CC'TnA_GG Cuts at: 2220 2220 Size: 6520 BtgI C'CryG_G Cuts at: 513 1065 2022 2856 513 Size: 552 957 834 4177 Fragments arranged by size: 4177 957 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5781 Fragments arranged by size: 5781 683 56 BtsI GCAGTG_nn' Cuts at: 2175 2633 4036 4830 4850 2175 Size: 458 1403 794 20 3845 Fragments arranged by size: 3845 1403 794 458 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2040 2493 Size: 54 1425 50 275 18 198 12 453 Cuts at: 2493 2859 3967 4856 6485 8 Size: 366 1108 889 1629 43 Fragments arranged by size: 1629 1425 1108 889 453 366 275 198 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2028 2040 3967 1537 Size: 275 216 12 1927 4090 Fragments arranged by size: 4090 1927 275 216 12 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2220 3210 4450 1353 Size: 734 133 990 1240 3423 Fragments arranged by size: 3423 1240 990 734 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5107 5481 5627 387 Size: 1064 138 3518 374 146 1280 Fragments arranged by size: 3518 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2149 2149 Size: 6520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2303 2904 3291 4583 5180 5967 Size: 393 479 192 601 387 1292 597 787 Cuts at: 5967 6235 1239 Size: 268 1524 Fragments arranged by size: 1524 1292 787 601 597 479 393 387 268 192 EcoO109I rG'GnC_Cy Cuts at: 2216 2216 Size: 6520 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 EcoRV GAT'ATC Cuts at: 3298 3298 Size: 6520 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 6520 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 3478 5667 1678 Size: 1800 2189 2531 Fragments arranged by size: 2531 2189 1800 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2809 2841 3001 3033 5161 Size: 32 135 32 1237 32 160 32 2128 Cuts at: 5161 5193 5235 5267 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2128 1237 160 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3864 4110 669 Size: 988 281 1240 686 246 3079 Fragments arranged by size: 3079 1240 988 686 281 246 HindIII A'AGCT_T Cuts at: 748 3359 748 Size: 2611 3909 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3729 5634 5818 1305 Size: 2424 1905 184 2007 Fragments arranged by size: 2424 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 2861 10 Size: 54 1425 343 663 366 3669 Fragments arranged by size: 3669 1425 663 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3147 4478 4837 4996 518 Size: 648 1981 1331 359 159 2042 Fragments arranged by size: 2042 1981 1331 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2281 3547 3656 3936 4613 5761 6006 1606 Size: 675 1266 109 280 677 1148 245 2120 Fragments arranged by size: 2120 1266 1148 677 675 280 245 109 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5011 Fragments arranged by size: 5011 957 552 NdeI CA'TA_TG Cuts at: 387 2655 387 Size: 2268 4252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 3878 5423 1132 Size: 97 2649 1545 2229 Fragments arranged by size: 2649 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2846 3232 1348 Size: 1498 386 4636 Fragments arranged by size: 4636 1498 386 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6520 PfoI T'CCnGG_A Cuts at: 3375 3375 Size: 6520 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 4589 4621 6102 6134 3587 Size: 32 970 32 1481 32 3973 Fragments arranged by size: 3973 1481 970 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2216 2216 Size: 6520 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 2511 4090 2511 Size: 1579 4941 PstI C_TGCA'G Cuts at: 830 2370 3586 3872 830 Size: 1540 1216 286 3478 Fragments arranged by size: 3478 1540 1216 286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2281 3656 2281 Size: 1375 5145 SacI G_AGCT'C Cuts at: 2151 2151 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SapI GCTCTTCn'nnn_ Cuts at: 3210 3210 Size: 6520 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 3723 4768 6480 3723 Size: 1045 1712 3763 Fragments arranged by size: 3763 1712 1045 SfcI C'TryA_G Cuts at: 826 1045 2366 3582 3868 5003 5684 5875 826 Size: 219 1321 1216 286 1135 681 191 1471 Fragments arranged by size: 1471 1321 1216 1135 681 286 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3159 3159 Size: 6520 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 3847 1872 Size: 1975 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3221 4624 5525 5787 820 Size: 197 926 1278 1403 901 262 1553 Fragments arranged by size: 1553 1403 1278 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3817 4311 493 Size: 3324 494 2702 Fragments arranged by size: 3324 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6520 StyI C'CwwG_G Cuts at: 513 1065 2022 3570 3576 3959 513 Size: 552 957 1548 6 383 3074 Fragments arranged by size: 3074 1548 957 552 383 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3159 3432 4547 4564 4717 4902 5347 Size: 1255 1579 273 1115 17 153 185 445 Cuts at: 5347 325 Size: 1498 Fragments arranged by size: 1579 1498 1255 1115 445 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 3721 4766 Size: 274 80 33 51 1443 1646 98 1045 Cuts at: 4766 6478 96 Size: 1712 138 Fragments arranged by size: 1712 1646 1443 1045 274 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2301 2333 2460 2492 6480 6512 2301 Size: 32 127 32 3988 32 2309 Fragments arranged by size: 3988 2309 127 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3781 3781 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 3845 1870 Size: 1975 4545 XmnI GAAnn'nnTTC Cuts at: 2529 3723 4649 2529 Size: 1194 926 4400 Fragments arranged by size: 4400 1194 926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI