Restriction map for pFN21ASDA0070
 (Linear) (Six-base) MAPSORT of: KIAA0070.seq  Check: 5950  from: 1  to: 1791



KIAA0070.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     350    1552    1791 
   Size:        350    1202     239

  Fragments arranged by size: 

                1202     350     239
 
AccI GT'mk_AC

Cuts at:      0     302    1495    1791 
   Size:        302    1193     296

  Fragments arranged by size: 

                1193     302     296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     280    1268    1791 
   Size:         88     192     988     523

  Fragments arranged by size: 

                 988     523     192      88
 
AfeI AGC'GCT

Cuts at:      0    1453    1791 
   Size:       1453     338
 
ApoI r'AATT_y

Cuts at:      0     502     727    1262    1791 
   Size:        502     225     535     529

  Fragments arranged by size: 

                 535     529     502     225
 
AseI AT'TA_AT

Cuts at:      0     515    1791 
   Size:        515    1276
 
BamHI G'GATC_C

Cuts at:      0     392    1791 
   Size:        392    1399
 
BanI G'GyrC_C

Cuts at:      0    1424    1791 
   Size:       1424     367
 
BanII G_rGCy'C

Cuts at:      0     128    1411    1791 
   Size:        128    1283     380

  Fragments arranged by size: 

                1283     380     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281    1733    1791 
   Size:        281    1452      58

  Fragments arranged by size: 

                1452     281      58
 
BclI T'GATC_A

Cuts at:      0    1389    1791 
   Size:       1389     402
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13    1136    1791 
   Size:         13    1123     655

  Fragments arranged by size: 

                1123     655      13
 
BlpI GC'TnA_GC

Cuts at:      0     142     586    1791 
   Size:        142     444    1205

  Fragments arranged by size: 

                1205     444     142
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     911    1791 
   Size:        911     880
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881    1791 
   Size:        881     910
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1219    1791 
   Size:       1219     572
 
BsaBI GATnn'nnATC

Cuts at:      0     814    1791 
   Size:        814     977
 
BsaHI Gr'CG_yC

Cuts at:      0      75     957    1791 
   Size:         75     882     834

  Fragments arranged by size: 

                 882     834      75
 
BsaWI w'CCGG_w

Cuts at:      0     965    1791 
   Size:        965     826
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     437     467    1791 
   Size:        437      30    1324

  Fragments arranged by size: 

                1324     437      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     458    1791 
   Size:        458    1333
 
BseYI C'CCAG_C

Cuts at:      0     780    1637    1791 
   Size:        780     857     154

  Fragments arranged by size: 

                 857     780     154
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    1791 
   Size:         31    1760
 
BsiEI CG_ry'CG

Cuts at:      0       8      20     939    1791 
   Size:          8      12     919     852

  Fragments arranged by size: 

                 919     852      12       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     128    1791 
   Size:        128    1663
 
BsmI GAATG_Cn'

Cuts at:      0    1303    1791 
   Size:       1303     488
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      66    1791 
   Size:         66    1725
 
Bsp1286I G_dGCh'C

Cuts at:      0     128    1411    1791 
   Size:        128    1283     380

  Fragments arranged by size: 

                1283     380     128
 
BspEI T'CCGG_A

Cuts at:      0     965    1791 
   Size:        965     826
 
BspHI T'CATG_A

Cuts at:      0    1469    1791 
   Size:       1469     322
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     350     376    1552    1791 
   Size:        350      26    1176     239

  Fragments arranged by size: 

                1176     350     239      26
 
BsrBI CCG'CTC

Cuts at:      0     740    1430    1791 
   Size:        740     690     361

  Fragments arranged by size: 

                 740     690     361
 
BsrDI GCAATG_nn'

Cuts at:      0    1494    1791 
   Size:       1494     297
 
BstEII G'GTnAC_C

Cuts at:      0    1110    1791 
   Size:       1110     681
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1126    1791 
   Size:       1126     665
 
BstYI r'GATC_y

Cuts at:      0     392    1791 
   Size:        392    1399
 
BstZ17I GTA'TAC

Cuts at:      0    1496    1791 
   Size:       1496     295
 
Bsu36I CC'TnA_GG

Cuts at:      0     197    1791 
   Size:        197    1594
 
BtgI C'CryG_G

Cuts at:      0     833    1791 
   Size:        833     958
 
BtsI GCAGTG_nn'

Cuts at:      0     152     610    1791 
   Size:        152     458    1181

  Fragments arranged by size: 

                1181     458     152
 
EaeI y'GGCC_r

Cuts at:      0       5      17     470     836    1791 
   Size:          5      12     453     366     955

  Fragments arranged by size: 

                 955     453     366      12       5
 
EagI C'GGCC_G

Cuts at:      0       5      17    1791 
   Size:          5      12    1774

  Fragments arranged by size: 

                1774      12       5
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     197    1187    1791 
   Size:         64     133     990     604

  Fragments arranged by size: 

                 990     604     133      64
 
EcoICRI GAG'CTC

Cuts at:      0     126    1791 
   Size:        126    1665
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     280     881    1268    1791 
   Size:         88     192     601     387     523

  Fragments arranged by size: 

                 601     523     387     192      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0     193    1791 
   Size:        193    1598
 
EcoRV GAT'ATC

Cuts at:      0    1275    1791 
   Size:       1275     516
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     444    1791 
   Size:        444    1347
 
HaeII r_GCGC'y

Cuts at:      0    1455    1791 
   Size:       1455     336
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     786     818     978    1010    1791 
   Size:        786      32     160      32     781

  Fragments arranged by size: 

                 786     781     160      32      32
 
HincII GTy'rAC

Cuts at:      0    1155    1791 
   Size:       1155     636
 
HindIII A'AGCT_T

Cuts at:      0    1336    1791 
   Size:       1336     455
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1706    1791 
   Size:       1706      85
 
MscI TGG'CCA

Cuts at:      0     472     838    1791 
   Size:        472     366     953

  Fragments arranged by size: 

                 953     472     366
 
MslI CAynn'nnrTG

Cuts at:      0    1124    1791 
   Size:       1124     667
 
MspA1I CmG'CkG

Cuts at:      0     258    1524    1633    1791 
   Size:        258    1266     109     158

  Fragments arranged by size: 

                1266     258     158     109
 
NdeI CA'TA_TG

Cuts at:      0     632    1791 
   Size:        632    1159
 
PasI CC'CwG_GG

Cuts at:      0     823    1209    1791 
   Size:        823     386     582

  Fragments arranged by size: 

                 823     582     386
 
PfoI T'CCnGG_A

Cuts at:      0    1352    1791 
   Size:       1352     439
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    1791 
   Size:       1564      32     195

  Fragments arranged by size: 

                1564     195      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     193    1791 
   Size:        193    1598
 
PsiI TTA'TAA

Cuts at:      0     488    1791 
   Size:        488    1303
 
PstI C_TGCA'G

Cuts at:      0     347    1563    1791 
   Size:        347    1216     228

  Fragments arranged by size: 

                1216     347     228
 
PvuII CAG'CTG

Cuts at:      0     258    1633    1791 
   Size:        258    1375     158

  Fragments arranged by size: 

                1375     258     158
 
SacI G_AGCT'C

Cuts at:      0     128    1791 
   Size:        128    1663
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1187    1791 
   Size:       1187     604
 
ScaI AGT'ACT

Cuts at:      0    1700    1791 
   Size:       1700      91
 
SfcI C'TryA_G

Cuts at:      0     343    1559    1791 
   Size:        343    1216     232

  Fragments arranged by size: 

                1216     343     232
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1136    1791 
   Size:       1136     655
 
SmlI C'TyrA_G

Cuts at:      0    1198    1791 
   Size:       1198     593
 
StyI C'CwwG_G

Cuts at:      0    1547    1553    1791 
   Size:       1547       6     238

  Fragments arranged by size: 

                1547     238       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1136    1409    1791 
   Size:       1136     273     382

  Fragments arranged by size: 

                1136     382     273
 
TatI w'GTAC_w

Cuts at:      0    1600    1698    1791 
   Size:       1600      98      93

  Fragments arranged by size: 

                1600      98      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     278     310     437     469    1791 
   Size:        278      32     127      32    1322

  Fragments arranged by size: 

                1322     278     127      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1758    1791 
   Size:       1758      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     506    1700    1791 
   Size:        506    1194      91

  Fragments arranged by size: 

                1194     506      91
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     ApoI     AseI    BamHI     BanI
    BanII     BbsI     BclI     BglI     BlpI     BplI     BpmI    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I
     NdeI     PasI     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SfiI     SmlI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsiWI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI    EcoNI    EcoRI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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