(Circular) (Six-base) MAPSORT of: pFN21ASDA0065.seq Check: 5508 from: 1 to: 7159 pFN21ASDA0065 7159 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4969 278 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4502 1937 Size: 2565 4594 Acc65I G'GTAC_C Cuts at: 4480 4480 Size: 7159 AclI AA'CG_TT Cuts at: 1563 5286 5659 1563 Size: 3723 373 3063 Fragments arranged by size: 3723 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 2960 4108 5222 6606 6874 1632 Size: 559 769 1148 1114 1384 268 1917 Fragments arranged by size: 1917 1384 1148 1114 769 559 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6962 AflIII A'CryG_T Cuts at: 1203 6058 7035 1203 Size: 4855 977 1327 Fragments arranged by size: 4855 1327 977 AgeI A'CCGG_T Cuts at: 5990 5990 Size: 7159 AhdI GACnn_n'nnGTC Cuts at: 5888 5888 Size: 7159 AleI CACnn'nnGTG Cuts at: 1166 3411 1166 Size: 2245 4914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7159 AlwNI CAG_nnn'CTG Cuts at: 2107 4902 6474 2107 Size: 2795 1572 2792 Fragments arranged by size: 2795 2792 1572 ApaLI G'TGCA_C Cuts at: 3068 5216 6372 3068 Size: 2148 1156 3855 Fragments arranged by size: 3855 2148 1156 ApoI r'AATT_y Cuts at: 1493 2249 2525 2771 2846 3486 3698 3906 4118 Size: 756 276 246 75 640 212 208 212 Cuts at: 4118 4369 4468 4694 4846 6955 1493 Size: 251 99 226 152 2109 1697 Fragments arranged by size: 2109 1697 756 640 276 251 246 226 212 212 208 152 99 75 AseI AT'TA_AT Cuts at: 160 1032 5713 160 Size: 872 4681 1606 Fragments arranged by size: 4681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 AvaI C'yCGr_G Cuts at: 1870 1943 4484 1870 Size: 73 2541 4545 Fragments arranged by size: 4545 2541 73 BamHI G'GATC_C Cuts at: 4489 4856 4489 Size: 367 6792 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4480 5935 618 Size: 325 217 627 2693 1455 1842 Fragments arranged by size: 2693 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 4162 4478 1391 Size: 737 2034 316 4072 Fragments arranged by size: 4072 2034 737 316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4333 928 Size: 971 2434 3754 Fragments arranged by size: 3754 2434 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5350 5384 1903 Size: 34 3413 34 3678 Fragments arranged by size: 3678 3413 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5051 6261 5051 Size: 1210 5949 BclI T'GATC_A Cuts at: 1548 2284 1548 Size: 736 6423 BfrBI ATG'CAT Cuts at: 3521 3521 Size: 7159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5770 136 Size: 107 122 71 631 605 4098 1525 Fragments arranged by size: 4098 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7154 7154 Size: 7159 BlpI GC'TnA_GC Cuts at: 4576 4576 Size: 7159 Bme1580I G_kGCm'C Cuts at: 1790 3072 5220 6376 1790 Size: 1282 2148 1156 2573 Fragments arranged by size: 2573 2148 1282 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4890 5848 442 Size: 416 53 83 399 24 3473 958 1753 Fragments arranged by size: 3473 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7159 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 5819 1239 Size: 929 3651 2579 Fragments arranged by size: 3651 2579 929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2565 3455 5284 6149 6447 2565 Size: 890 1829 865 298 3277 Fragments arranged by size: 3277 1829 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5822 882 Size: 617 4323 2219 Fragments arranged by size: 4323 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4456 4950 493 Size: 711 3252 494 2702 Fragments arranged by size: 3252 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7153 7153 Size: 7159 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4966 5348 5983 275 Size: 53 83 186 936 3433 382 635 1451 Fragments arranged by size: 3433 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5592 5990 6264 6411 6822 5592 Size: 398 274 147 411 5929 Fragments arranged by size: 5929 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6934 Fragments arranged by size: 6934 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2314 2645 1186 Size: 922 206 331 5700 Fragments arranged by size: 5700 922 331 206 BseYI C'CCAG_C Cuts at: 6362 6858 6362 Size: 496 6663 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6586 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4609 5370 6398 1540 Size: 275 186 2608 761 1028 2301 Fragments arranged by size: 2608 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2670 3072 5220 5305 6376 2670 Size: 402 2148 85 1071 3453 Fragments arranged by size: 3453 2148 1071 402 85 BsmI GAATG_Cn' Cuts at: 3182 4106 4670 4763 3182 Size: 924 564 93 5578 Fragments arranged by size: 5578 924 564 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2670 3072 4162 4478 5220 5305 Size: 399 338 542 402 1090 316 742 85 Cuts at: 5305 6376 1391 Size: 1071 2174 Fragments arranged by size: 2174 1090 1071 742 542 402 399 338 316 85 BspEI T'CCGG_A Cuts at: 6822 6822 Size: 7159 BspHI T'CATG_A Cuts at: 2626 3769 5048 6778 2626 Size: 1143 1279 1730 3007 Fragments arranged by size: 3007 1730 1279 1143 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4514 844 Size: 3670 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5046 1394 Size: 429 3223 3507 Fragments arranged by size: 3507 3223 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3187 4111 5654 5828 65 Size: 1183 1939 924 1543 174 1396 Fragments arranged by size: 1939 1543 1396 1183 924 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5803 5990 1700 Size: 110 3993 187 2869 Fragments arranged by size: 3993 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3979 4315 96 Size: 3883 336 2940 Fragments arranged by size: 3883 2940 336 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7159 BssSI C'ACGA_G Cuts at: 5219 6231 5219 Size: 1012 6147 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7159 BstBI TT'CG_AA Cuts at: 2007 5963 2007 Size: 3956 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7159 BstYI r'GATC_y Cuts at: 1972 4489 4856 5241 5258 6710 6796 6808 7154 Size: 2517 367 385 17 1452 86 12 346 Cuts at: 7154 1972 Size: 1977 Fragments arranged by size: 2517 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2231 513 Size: 552 1166 5441 Fragments arranged by size: 5441 1166 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6420 Fragments arranged by size: 6420 683 56 BtsI GCAGTG_nn' Cuts at: 4675 5469 5489 4675 Size: 794 20 6345 Fragments arranged by size: 6345 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3169 3204 4093 4128 529 Size: 35 2605 35 889 35 3560 Fragments arranged by size: 3560 2605 889 35 35 35 DraI TTT'AAA Cuts at: 2065 4463 2065 Size: 2398 4761 DraIII CAC_nnn'GTG Cuts at: 4418 4418 Size: 7159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6166 809 Size: 507 4850 1802 Fragments arranged by size: 4850 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4606 5495 7124 Size: 54 1425 50 275 18 2776 889 1629 Cuts at: 7124 8 Size: 43 Fragments arranged by size: 2776 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4606 1537 Size: 275 2794 4090 Fragments arranged by size: 4090 2794 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 2726 2742 5089 1353 Size: 867 506 16 2347 3423 Fragments arranged by size: 3423 2347 867 506 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5746 6120 6266 387 Size: 1064 138 4157 374 146 1280 Fragments arranged by size: 4157 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2191 2960 4108 5222 5819 6606 Size: 393 536 23 769 1148 1114 597 787 Cuts at: 6606 6874 1239 Size: 268 1524 Fragments arranged by size: 1524 1148 1114 787 769 597 536 393 268 23 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2708 Size: 7159 EcoRI G'AATT_C Cuts at: 2249 3486 3906 4468 4846 2249 Size: 1237 420 562 378 4562 Fragments arranged by size: 4562 1237 562 420 378 EcoRV GAT'ATC Cuts at: 2820 2820 Size: 7159 FspI TGC'GCA Cuts at: 5665 5665 Size: 7159 HaeII r_GCGC'y Cuts at: 1678 6306 1678 Size: 4628 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2269 2301 2783 Size: 32 135 32 470 32 195 32 482 Cuts at: 2783 2815 5800 5832 5874 5906 1373 Size: 32 2985 32 42 32 2626 Fragments arranged by size: 2985 2626 482 470 195 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3717 4503 4749 669 Size: 988 281 1779 786 246 3079 Fragments arranged by size: 3079 1779 988 786 281 246 HindIII A'AGCT_T Cuts at: 748 2263 3027 3111 3951 748 Size: 1515 764 84 840 3956 Fragments arranged by size: 3956 1515 840 764 84 HpaI GTT'AAC Cuts at: 4749 4749 Size: 7159 KpnI G_GTAC'C Cuts at: 4484 4484 Size: 7159 MfeI C'AATT_G Cuts at: 4758 4758 Size: 7159 MluI A'CGCG_T Cuts at: 7035 7035 Size: 7159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6273 6457 1305 Size: 4968 184 2007 Fragments arranged by size: 4968 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5337 Fragments arranged by size: 5337 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3411 3774 5117 5476 5635 518 Size: 648 2245 363 1343 359 159 2042 Fragments arranged by size: 2245 2042 1343 648 363 359 159 MspA1I CmG'CkG Cuts at: 1606 2282 4575 5252 6400 6645 1606 Size: 676 2293 677 1148 245 2120 Fragments arranged by size: 2293 2120 1148 677 676 245 NcoI C'CATG_G Cuts at: 513 1065 2231 513 Size: 552 1166 5441 Fragments arranged by size: 5441 1166 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7159 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7159 NotI GC'GGCC_GC Cuts at: 4606 4606 Size: 7159 NsiI A_TGCA'T Cuts at: 3523 3523 Size: 7159 NspI r_CATG'y Cuts at: 1132 1229 3525 3684 4281 4517 6062 1132 Size: 97 2296 159 597 236 1545 2229 Fragments arranged by size: 2296 2229 1545 597 236 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7159 PciI A'CATG_T Cuts at: 6058 6058 Size: 7159 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7159 PmeI GTTT'AAAC Cuts at: 4463 4463 Size: 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5228 5260 6741 6773 5228 Size: 32 1481 32 5614 Fragments arranged by size: 5614 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2115 1613 Size: 502 6657 PsiI TTA'TAA Cuts at: 2766 4729 2766 Size: 1963 5196 PstI C_TGCA'G Cuts at: 830 4511 830 Size: 3681 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7159 PvuII CAG'CTG Cuts at: 2282 2282 Size: 7159 SalI G'TCGA_C Cuts at: 1936 4501 1936 Size: 2565 4594 SapI GCTCTTCn'nnn_ Cuts at: 2220 2220 Size: 7159 SbfI CC_TGCA'GG Cuts at: 4511 4511 Size: 7159 ScaI AGT'ACT Cuts at: 5407 7119 5407 Size: 1712 5447 SfcI C'TryA_G Cuts at: 826 1045 2155 4507 5642 6323 6514 826 Size: 219 1110 2352 1135 681 191 1471 Fragments arranged by size: 2352 1471 1135 1110 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7159 SmaI CCC'GGG Cuts at: 1872 4486 1872 Size: 2614 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2580 3434 5263 6164 6426 820 Size: 197 926 637 854 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 854 637 262 197 SnaBI TAC'GTA Cuts at: 493 4456 4950 493 Size: 3963 494 2702 Fragments arranged by size: 3963 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7159 SphI G_CATG'C Cuts at: 1132 4517 1132 Size: 3385 3774 SspI AAT'ATT Cuts at: 5 52 2557 5083 5 Size: 47 2505 2526 2081 Fragments arranged by size: 2526 2505 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7159 StyI C'CwwG_G Cuts at: 513 1065 2231 4598 513 Size: 552 1166 2367 3074 Fragments arranged by size: 3074 2367 1166 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 5186 5203 5356 5541 5986 325 Size: 1255 522 3084 17 153 185 445 1498 Fragments arranged by size: 3084 1498 1255 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3391 3643 3979 Size: 274 80 33 51 1443 1414 252 336 Cuts at: 3979 4147 4315 5405 7117 96 Size: 168 168 1090 1712 138 Fragments arranged by size: 1712 1443 1414 1090 336 274 252 168 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7119 7151 7119 Size: 32 7127 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7159 XbaI T'CTAG_A Cuts at: 4495 4495 Size: 7159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7159 XmaI C'CCGG_G Cuts at: 1870 4484 1870 Size: 2614 4545 XmnI GAAnn'nnTTC Cuts at: 2945 3029 3533 3617 4121 4289 5288 2945 Size: 84 504 84 504 168 999 4816 Fragments arranged by size: 4816 999 504 504 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4967 276 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI