(Circular) (Six-base) MAPSORT of: pFN21ASDA0053.seq Check: 1322 from: 1 to: 6643 pFN21ASDA0053 6643 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4453 278 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3986 1937 Size: 2049 4594 Acc65I G'GTAC_C Cuts at: 3964 3964 Size: 6643 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 3172 3177 4706 6090 6358 1632 Size: 548 992 5 1529 1384 268 1917 Fragments arranged by size: 1917 1529 1384 992 548 268 5 AfeI AGC'GCT Cuts at: 2480 3845 2480 Size: 1365 5278 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 5542 6519 1203 Size: 4339 977 1327 Fragments arranged by size: 4339 1327 977 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 2681 1166 Size: 1515 5128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2115 1184 Size: 931 5712 AlwNI CAG_nnn'CTG Cuts at: 4386 5958 4386 Size: 1572 5071 ApaLI G'TGCA_C Cuts at: 2684 3552 4700 5856 2684 Size: 868 1148 1156 3471 Fragments arranged by size: 3471 1156 1148 868 ApoI r'AATT_y Cuts at: 1493 2432 2510 3168 3404 3899 3952 4178 4330 Size: 939 78 658 236 495 53 226 152 Cuts at: 4330 6439 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 939 658 495 236 226 152 78 53 AseI AT'TA_AT Cuts at: 160 1032 5197 160 Size: 872 4165 1606 Fragments arranged by size: 4165 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3849 3968 1870 Size: 73 1906 119 4545 Fragments arranged by size: 4545 1906 119 73 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2527 3368 3964 5419 618 Size: 325 217 627 740 841 596 1455 1842 Fragments arranged by size: 1842 1455 841 740 627 596 325 217 BanII G_rGCy'C Cuts at: 1391 2736 3962 1391 Size: 1345 1226 4072 Fragments arranged by size: 4072 1345 1226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 3165 928 Size: 971 1086 180 4406 Fragments arranged by size: 4406 1086 971 180 BbvCI CC'TCA_GC Cuts at: 2210 2210 Size: 6643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2966 3000 4834 4868 1903 Size: 34 1029 34 1834 34 3678 Fragments arranged by size: 3678 1834 1029 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4535 5745 4535 Size: 1210 5433 BclI T'GATC_A Cuts at: 1548 2991 3036 1548 Size: 1443 45 5155 Fragments arranged by size: 5155 1443 45 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5254 136 Size: 107 122 71 631 605 3582 1525 Fragments arranged by size: 3582 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3390 6638 3390 Size: 3248 3395 BlpI GC'TnA_GC Cuts at: 2808 4060 2808 Size: 1252 5391 Bme1580I G_kGCm'C Cuts at: 1790 2532 2688 3447 3556 4704 5860 1790 Size: 742 156 759 109 1148 1156 2573 Fragments arranged by size: 2573 1156 1148 759 742 156 109 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4374 5332 Size: 416 53 83 399 24 2207 750 958 Cuts at: 5332 442 Size: 1753 Fragments arranged by size: 2207 1753 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2677 3021 2677 Size: 344 6299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2585 2767 3105 3657 3809 5303 1239 Size: 914 432 182 338 552 152 1494 2579 Fragments arranged by size: 2579 1494 914 552 432 338 182 152 Bpu10I CC'TnA_GC Cuts at: 2210 2864 3429 2210 Size: 654 565 5424 Fragments arranged by size: 5424 654 565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3447 3755 4768 5633 5931 3447 Size: 308 1013 865 298 4159 Fragments arranged by size: 4159 1013 865 308 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2715 3036 5306 882 Size: 617 1216 321 2270 2219 Fragments arranged by size: 2270 2219 1216 617 321 BsaAI yAC'GTr Cuts at: 493 1204 3940 4434 493 Size: 711 2736 494 2702 Fragments arranged by size: 2736 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6637 6637 Size: 6643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4450 4832 5467 275 Size: 53 83 186 936 2917 382 635 1451 Fragments arranged by size: 2917 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3227 3253 3336 5076 5474 5748 5895 6306 3227 Size: 26 83 1740 398 274 147 411 3564 Fragments arranged by size: 3564 1740 411 398 274 147 83 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6418 Fragments arranged by size: 6418 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 2997 1186 Size: 1249 562 4832 Fragments arranged by size: 4832 1249 562 BseYI C'CCAG_C Cuts at: 2321 2648 3144 5846 6342 2321 Size: 327 496 2702 496 2622 Fragments arranged by size: 2702 2622 496 496 327 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2571 1163 Size: 573 835 5235 Fragments arranged by size: 5235 835 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2575 4093 4854 5882 1540 Size: 275 186 574 1518 761 1028 2301 Fragments arranged by size: 2301 1518 1028 761 574 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 2688 3556 4704 4789 5860 2571 Size: 117 868 1148 85 1071 3354 Fragments arranged by size: 3354 1148 1071 868 117 85 BsmI GAATG_Cn' Cuts at: 2794 4154 4247 2794 Size: 1360 93 5190 Fragments arranged by size: 5190 1360 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2628 2628 Size: 6643 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2532 2571 2688 2736 3447 3556 3962 Size: 399 742 39 117 48 711 109 406 Cuts at: 3962 4704 4789 5860 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 742 711 406 399 117 109 85 48 39 BspEI T'CCGG_A Cuts at: 3253 6306 3253 Size: 3053 3590 BspHI T'CATG_A Cuts at: 2994 4532 6262 2994 Size: 1538 1730 3375 Fragments arranged by size: 3375 1730 1538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2868 3998 844 Size: 2024 1130 3489 Fragments arranged by size: 3489 2024 1130 BsrBI CCG'CTC Cuts at: 1394 1823 2656 4530 1394 Size: 429 833 1874 3507 Fragments arranged by size: 3507 1874 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5138 5312 65 Size: 1183 3890 174 1396 Fragments arranged by size: 3890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5287 5474 1700 Size: 110 3477 187 2869 Fragments arranged by size: 3477 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2285 96 Size: 2189 4454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 2844 4703 5715 2844 Size: 1859 1012 3772 Fragments arranged by size: 3772 1859 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 2007 5447 2007 Size: 3440 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2149 2355 2539 3294 2131 Size: 18 206 184 755 5480 Fragments arranged by size: 5480 755 206 184 18 BstYI r'GATC_y Cuts at: 1972 3012 3390 3973 4340 4725 4742 6194 6280 Size: 1040 378 583 367 385 17 1452 86 Cuts at: 6280 6292 6638 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1040 583 385 378 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2438 2438 Size: 6643 BtgI C'CryG_G Cuts at: 513 1065 2736 513 Size: 552 1671 4420 Fragments arranged by size: 4420 1671 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3258 487 Size: 683 56 2032 3872 Fragments arranged by size: 3872 2032 683 56 BtsI GCAGTG_nn' Cuts at: 4159 4953 4973 4159 Size: 794 20 5829 Fragments arranged by size: 5829 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3397 3947 3397 Size: 550 6093 DraIII CAC_nnn'GTG Cuts at: 2462 2692 2462 Size: 230 6413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2387 3050 5650 809 Size: 507 1071 663 2600 1802 Fragments arranged by size: 2600 1802 1071 663 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2473 2572 Size: 54 1425 50 275 18 567 76 99 Cuts at: 2572 4090 4979 6608 8 Size: 1518 889 1629 43 Fragments arranged by size: 1629 1518 1425 889 567 275 99 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2572 4090 1537 Size: 275 760 1518 4090 Fragments arranged by size: 4090 1518 760 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2577 3062 3565 3636 3860 4573 1353 Size: 885 339 485 503 71 224 713 3423 Fragments arranged by size: 3423 885 713 503 485 339 224 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2426 5230 5604 5750 387 Size: 1064 138 837 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 837 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2180 2585 2767 3105 3172 3177 Size: 393 521 27 405 182 338 67 5 Cuts at: 3177 3657 3809 4706 5303 6090 6358 1239 Size: 480 152 897 597 787 268 1524 Fragments arranged by size: 1524 897 787 597 521 480 405 393 338 268 182 152 67 27 5 EcoNI CCTnn'n_nnAGG Cuts at: 3737 3737 Size: 6643 EcoO109I rG'GnC_Cy Cuts at: 3531 3531 Size: 6643 EcoRI G'AATT_C Cuts at: 3404 3952 4330 3404 Size: 548 378 5717 Fragments arranged by size: 5717 548 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 6643 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 2482 3847 5790 1678 Size: 804 1365 1943 2531 Fragments arranged by size: 2531 1943 1365 804 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2274 2306 5284 5316 5358 Size: 32 135 32 702 32 2978 32 42 Cuts at: 5358 5390 1373 Size: 32 2626 Fragments arranged by size: 2978 2626 702 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2914 3987 4233 669 Size: 988 281 976 1073 246 3079 Fragments arranged by size: 3079 1073 988 976 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6643 HpaI GTT'AAC Cuts at: 2914 4233 2914 Size: 1319 5324 KpnI G_GTAC'C Cuts at: 3968 3968 Size: 6643 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5757 5941 1305 Size: 4452 184 2007 Fragments arranged by size: 4452 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 2475 10 Size: 54 1425 343 567 76 4178 Fragments arranged by size: 4178 1425 567 343 76 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2537 2681 4601 4960 5119 518 Size: 648 981 390 144 1920 359 159 2042 Fragments arranged by size: 2042 1920 981 648 390 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2131 2629 2899 4059 4736 5884 6129 1606 Size: 525 498 270 1160 677 1148 245 2120 Fragments arranged by size: 2120 1160 1148 677 525 498 270 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6091 NdeI CA'TA_TG Cuts at: 387 387 Size: 6643 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 2272 4001 5546 1132 Size: 97 1043 1729 1545 2229 Fragments arranged by size: 2229 1729 1545 1043 97 PasI CC'CwG_GG Cuts at: 1348 2260 3276 3536 1348 Size: 912 1016 260 4455 Fragments arranged by size: 4455 1016 912 260 PciI A'CATG_T Cuts at: 5542 5542 Size: 6643 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 1108 Size: 1374 5269 PfoI T'CCnGG_A Cuts at: 2562 2562 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3445 3477 4712 4744 6225 6257 3445 Size: 32 1235 32 1481 32 3831 Fragments arranged by size: 3831 1481 1235 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3531 3531 Size: 6643 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 4213 4213 Size: 6643 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2747 2779 2747 Size: 32 6611 PstI C_TGCA'G Cuts at: 830 2879 3814 3995 830 Size: 2049 935 181 3478 Fragments arranged by size: 3478 2049 935 181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 PvuII CAG'CTG Cuts at: 2629 2899 2629 Size: 270 6373 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SanDI GG'GwC_CC Cuts at: 3531 3531 Size: 6643 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 4891 6603 4891 Size: 1712 4931 SexAI A'CCwGG_T Cuts at: 2616 2616 Size: 6643 SfcI C'TryA_G Cuts at: 826 1045 2058 2854 2875 3138 3810 3991 5126 Size: 219 1013 796 21 263 672 181 1135 Cuts at: 5126 5807 5998 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1013 796 681 672 263 219 191 181 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3851 3970 1872 Size: 1979 119 4545 Fragments arranged by size: 4545 1979 119 SmlI C'TyrA_G Cuts at: 820 1017 1943 3462 3734 4747 5648 5910 820 Size: 197 926 1519 272 1013 901 262 1553 Fragments arranged by size: 1553 1519 1013 926 901 272 262 197 SnaBI TAC'GTA Cuts at: 493 3940 4434 493 Size: 3447 494 2702 Fragments arranged by size: 3447 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 4001 1132 Size: 2869 3774 SspI AAT'ATT Cuts at: 5 52 4567 5 Size: 47 4515 2081 Fragments arranged by size: 4515 2081 47 StuI AGG'CCT Cuts at: 1517 3878 1517 Size: 2361 4282 StyI C'CwwG_G Cuts at: 513 1065 3069 3579 3879 4082 513 Size: 552 2004 510 300 203 3074 Fragments arranged by size: 3074 2004 552 510 300 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2021 2055 3944 4670 4687 4840 5025 Size: 1255 441 34 1889 726 17 153 185 Cuts at: 5025 5470 325 Size: 445 1498 Fragments arranged by size: 1889 1498 1255 726 445 441 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2285 4889 6601 Size: 274 80 33 51 1443 308 2604 1712 Cuts at: 6601 96 Size: 138 Fragments arranged by size: 2604 1712 1443 308 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6603 6635 6603 Size: 32 6611 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 3849 3968 1870 Size: 1979 119 4545 Fragments arranged by size: 4545 1979 119 XmnI GAAnn'nnTTC Cuts at: 2343 3344 4772 2343 Size: 1001 1428 4214 Fragments arranged by size: 4214 1428 1001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4451 276 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI