Restriction map for
pFN21ASDA0050
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspHI
AviII AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BlnI BpuAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 494 GGTACC G^GTACC
AflII 774 CTTAAG C^TTAAG
AflIII 723 ACACGT A^C[GA][CT]GT
Asp700I 1548 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 494 GGTACC G^GTACC
AspI 2059 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 774 CTTAAG C^TTAAG
BglI 445 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1448 AGATCT A^GATCT
BsaWI 1370 TCCGGT [AT]^CCGG[AT]
BsmI 1319 GCATTC GAATGC(1/-1)
BspHI 2202 TCATGA T^CATGA
BsrFI 632 GCCGGC [GA]^CCGG[CT]
BssSI 695 CACGAG CACGAG(-5/-1)
BstXI 2033 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 632 GCCGGC [GA]^CCGG[CT]
DraIII 1216 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 687 GAATTC G^AATTC
HincII 1792 GTCAAC GT[CT]^[GA]AC
HindII 1792 GTCAAC GT[CT]^[GA]AC
HindIII 771 AAGCTT A^AGCTT
KpnI 494 GGTACC GGTAC^C
NaeI 632 GCCGGC GCC^GGC
NgoMIV 632 GCCGGC G^CCGGC
PflFI 2059 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2202 TCATGA T^CATGA
SmaI 1115 CCCGGG CCC^GGG
SpeI 1404 ACTAGT A^CTAGT
StuI 1520 AGGCCT AGG^CCT
Tth111I 2059 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 291 TCTAGA T^CTAGA
XmaCI 1115 CCCGGG C^CCGGG
XmaI 1115 CCCGGG C^CCGGG
XmnI 1548 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 244 AAATTC [GA]^AATT[CT]
687 GAATTC
AfaI 495 GTAC GT^AC
900 GTAC
ApoI 244 AAATTC [GA]^AATT[CT]
687 GAATTC
BbrPI 724 CACGTG CAC^GTG
1216 CACGTG
BfaI 292 CTAG C^TAG
1405 CTAG
BlpI 627 GCTGAGC GC^T[ACGT]AGC
2187 GCTGAGC
Bpu1102I 627 GCTGAGC GC^T[ACGT]AGC
2187 GCTGAGC
BsmBI 937 CGTCTC CGTCTC(1/5)
2128 GAGACG
BsrDI 1074 CATTGC GCAATG(2/0)
1247 GCAATG
BstAPI 463 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1004 GCAAGTCCTGC
CelII 627 GCTGAGC GC^T[ACGT]AGC
2187 GCTGAGC
EcoNI 333 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1014 CCTCCTCCAGG
MaeI 292 CTAG C^TAG
1405 CTAG
MseI 775 TTAA T^TAA
839 TTAA
MslI 1473 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2034 CATCGCCATG
PaeR7I 485 CTCGAG C^TCGAG
2003 CTCGAG
PmaCI 724 CACGTG CAC^GTG
1216 CACGTG
PmlI 724 CACGTG CAC^GTG
1216 CACGTG
PshAI 330 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
931 GACCTTCGTC
RsaI 495 GTAC GT^AC
900 GTAC
TfiI 1527 GATTC G^A[AT]TC
1575 GATTC
Tru9I 775 TTAA T^TAA
839 TTAA
XhoI 485 CTCGAG C^TCGAG
2003 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII